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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: The expression of mRNA transcripts was determined using cDNA reverse-transcribed from total RNA and quantitative PCR (qPCR) was performed on the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA) using SYBR Green master mixes (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 104 MDCK-5KO-EKARrEV-NLS-loxP-NRG1 or MDCK-5KO-EKARrEV-NLS-loxP-NRG1-CreERT2 cells were seeded in a 6-well plate (No. 140675, Thermo Fisher Scientific, Waltham, MA) and cultured in DMEM containing 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells were seeded in 6-well plates (5×105/well) were transfected with the indicated siRNA (3 μg) using Lipofectamine® 2000 transfection reagent (Life Technologies, 11668-019) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents, Fisher Scientific) in a Fujifilm LAS-3000 imager ...
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bioRxiv - Synthetic Biology 2021Quote: ... 384-well square- or round-bottom microplates were used (Sup Table 6) and covered by an adhesive plate seal (Thermo Scientific #AB0558 or #232701) to minimize evaporation ...
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Immunology 2021Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed hearts were embedded in paraffin and sections (4-6 μm thickness) were mounted onto Superfrost® Plus microscope slides (Thermo scientific #630-0950). Immunohistochemistry was carried out using standard procedures ...
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bioRxiv - Cancer Biology 2022Quote: ... HN-6 cells were plated overnight at 40% confluence and transfected with siNC or siSMYD3 using Lipofectamine RNAimax (Thermo Fisher Scientific, Grand Island, NY) for 72h ...
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bioRxiv - Biochemistry 2022Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 g for 30 minutes, A23-6 x 100 rotor, (Thermo Fisher Scientific, Waltham, MA). The supernatant was filtrated with a Filtropur BT50 0.2 µm bottle top filter (SARSTEDT ...
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bioRxiv - Bioengineering 2022Quote: ... Rat bone marrow mononuclear cells were cultured at 4-6 million in a 10cm dish (Falcon) using differentiation media containing: DMEM (ThermoFisher Scientific, Burlington, ON, Canada), 20% HI FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, F9-6 x 1000 LEX rotor, Thermo Fisher Scientific, Waltham, MA). The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, F9-6 x 1000 LEX rotor, Thermo Fisher Scientific, Waltham, MA). Cells were washed once with water and suspended in 75 mL of ice-cold water ...
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bioRxiv - Plant Biology 2022Quote: ... respectively) were amplified by PCR from sunflower CAS-6 genomic DNA using the Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA) and they were cloned into the pMBL-T vector (CANVAX Biotech ...
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bioRxiv - Plant Biology 2022Quote: Replicate trials of soil grown grafted plants from 3-7 days post-grafting were cut at their root tips and dipped into (5 mg/mL) of 5(-and-6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen®, Waltham, MA, USA). Plants were incubated under full-spectrum LED lights (Cirrus LED Grow Lights ...
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bioRxiv - Neuroscience 2023Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin-labeled axons were histologically detected using Alexa Fluor 594-conjugated Streptavidin (Thermo Fisher, cat# S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the Fiji software ...
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bioRxiv - Physiology 2022Quote: ... maintenance medium was added to differentiated adipocytes on day 6 post-induction of differentiation (300,000 preadipocytes were plated per well of a 6-well plate before the induction of differentiation) was supplemented with 50 μM of ManAZ (Thermo Fisher Scientific, Cat #C33366) for 48h ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cDNA was run for quantitative PCR using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) kit on QuantStudio 6 and 7 Flex real-time PCR systems (Thermo Fisher, USA). Reagents mentioned above were obtained from Takara (Japan) ...
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bioRxiv - Developmental Biology 2022Quote: ... was placed into a 6-well tissue culture dish (0.8-1.0ml) and the 6-well plate fitted with Falcon™ Cell Culture Inserts with 8 um pore size (Fisher Scientific, 08-771-20). The lungs were transferred from the holding medium onto the membrane with a sterile razor blade and 5-10 ul of holding media to keep the lungs wet ...
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bioRxiv - Physiology 2022Quote: ... 125 mL of medium was centrifuged at 8000 rpm (9730 x g) for 15 min (Sorvall RC 6 Plus; Thermo Scientific, Waltham, MA, USA). The supernatant was discarded ...
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bioRxiv - Microbiology 2022Quote: THY medium was made using the premixed powders of Todd Hewitt Broth (BD Bacto Dehydrated Culture Media: Todd Hewitt Broth, Fisher Scientific, DF0492-17-6) and yeast extract (Thermo Scientific™ Yeast Extract ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Immunology 2024Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Evolutionary Biology 2024Quote: ... and 5 μL of SsoADV SYBR® Green Supermix (Bio Rad) in a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems™) and following ...
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bioRxiv - Biochemistry 2024Quote: ... 100 ng of cDNAs were amplified in 1X TaqMan™ Fast Advanced Master Mix and 1X TaqMan™ Assay in a 20-mL reaction using the QuantStudio 6 Flex real-time PCR Platform (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 6 min and 10 min parallel reaction monitoring (PRM) methods are described (UltiMate 3000 RSLCnano and PRM–QExactive Plus; Thermo Scientific, Waltham, MA, USA). Raw data were processed using TraceFinder 4.1 (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... KM-met released from CS-NP was quantified at 233 nm using a NanoDrop One microvolume UV-Vis spectrophotometer for up to 6 hours at room temperature (Thermofisher Scientific, Waltham, MA, USA). At the endpoint ...
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bioRxiv - Developmental Biology 2023Quote: Primary human limbal epithelial stem cells were isolated from healthy donors and cultivated in 6 well plates in KSFM medium (Cat. Nr. 17005042, Thermo Fisher, Gibco, Life Technologies, Paisley, UK) supplemented with 500 ng Epidermal growth factor (EGF) ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate was collected in duplicate from TCPS 6-well plates or Alvetex 12-well inserts following ten days of differentiation at 37°C by RIPA Buffer (Thermo Fisher: 50-152-132) containing 1% proteinase inhibitor (Sigma ...
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bioRxiv - Physiology 2023Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Genomics 2023Quote: ... Relative mRNA levels were determined using qPCR reactions performed on the QuantStudio™ 6 Flex Real-Time PCR System with TaqMan™ Fast Advanced Master Mix (Thermo Fisher Scientific) and taqman probes designed to Cdkn1a (Mm04205640_g1 ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were thawed and seeded at a cell density of 5*10^6 cells/ml in RPMI 1640 GlutaMAX™ culture medium (Gibco, Cat.No. 61870-010) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... were plated in 6 well plates and 24 h later each siRNA (17 nM) was transfected with Lipofectamine™ RNAiMAX (Thermo Fisher Scientific, 13778150) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time qPCR reactions were carried out in technical triplicates using SYBR Green with a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies Inc.). Ct (cycle threshold ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before being flash frozen in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before total RNA was isolated from cells by Purelink™ RNA Mini Kit (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... except for Fig 6 and Fig 7 which the recovered cells were counted by inclusion of counting beads (Accu-Check, Molecular Probes, Thermo Fisher Scientific) in the FACS sample according to manufacturer’s instructions and calculated using the equation ...
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bioRxiv - Biophysics 2023Quote: ... Transfection lasted for 6 hours at 37°C and was terminated by replacing the medium with fresh 37°C Opti-MEM® (Life Technologies, ThermoFisher Scientific) supplemented with 50 μg/mL Gentamycin (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: The inhibition of kinase activity mediated by phospholipids was determined using the following Z’-LYTE Kinase Assay Kits: Z’-LYTE Kinase Assay Kit - Ser/Thr 6 Peptide (Thermo Fisher Scientific, Cat# PV3179) used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Allele sizes were identified by fragment analysis using fluorescent primers (6-FAM, PET, NED, and VIC) and a 3730xl Genetic Analyzer with the LIZ-500 size standard (Applied Biosystems, operated by Genewiz). Alleles were called using Peak Scanner 2.0 (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: A live/dead assay on the cell-laden GelMa constructs (n=6 per condition) was performed using a LIVE/DEADTM Cell Imaging kit (Invitrogen, Waltham, Massachusetts, United States) 144 h after Gel synthesis ...
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bioRxiv - Cell Biology 2023Quote: siRNA probes targeting sfrp2 in human mesenchymal cells and frizzled-5 and -6 in AEC2 cells were obtained from Thermo Fisher (Supplemental Table 4). In brief ...
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bioRxiv - Immunology 2023Quote: ... Cells were counted using a Cellometer Auto T4 Automated Cell Counter (Nexcelom Bioscience LLC, MA, USA) and plated on 6 well-plates (Nunclon Delta Surface, Thermo Fisher Scientific; Cat #140675) precoated with 500 μg/mL poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... overnight incubation in a cold room (6-7°C) was carried and anti-mouse/rabbit secondary antibody Alexa-488 conjugated (Thermo Fisher Scientific, Waltham, MA) incubation was performed (1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of cDNAs were amplified in 1X TaqMan™ Fast Advanced Master Mix and 1X TaqMan™ Assay in a 20-µL reaction using the QuantStudio 6 Flex real-time PCR Platform (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... control siRNA or siRNA targeting ADAR1 (30 pmol per well in 6-well plate) and Lipofectamine RNAiMAX transfection reagent (9 µL; Cat. # 13778075; Thermo Fisher Scientific, Dreieich, Germany) were used to transfect the cells according to manufacturer’s instructions ...