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Citations for Thermo Fisher :
4001 - 4050 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 5x105 cells of the primary hepatocyte were seeded in a 6-well plate (matrigel coated) with 2mL Advanced DMEM/F-12 (Thermo Fisher, 12634010) supplemented with 10% fetal calf serum ...
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bioRxiv - Neuroscience 2022Quote: ... 2.8×106 neural progenitor cells were grown on glass coverslips in 6-well plate and loaded with 2 µM Fura-2/AM and Pluronic F127 in OptiMEM (Thermo Fisher Scientific) for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... D13+14 PT-enhanced organoids (triplicate wells per condition) were incubated in 4’,6-diamindino-2-phenylindole substrate (DAPI; 1:1000 [Thermo Fisher Scientific]) with 1:500 DRAQ7 dead cell label (Thermo Fisher Scientific] ...
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bioRxiv - Neuroscience 2022Quote: ... prior to washing (6×60 min PBST) and secondary incubation with species specific Alexafluor IgG secondary antibodies (60 min RT, Invitrogen; 1:500). Tissues were washed again in 0.1% PBST (6 × 60 min) ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were supplemented with 150 fmol of internal standard (yeast ADH1, Waters) and subjected to tandem mass tag (TMT)-labeling using TMT 6-plex reagents (Thermo Fisher Scientific). TMT 126-128 were used for the reference (Vehicle or WT ...
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bioRxiv - Genomics 2022Quote: ... To evaluate the effect of SOCS2 editing on inflammatory gene response quantitative polymerase chain reaction (qPCR) was performed on a QuantStudio™ 6 Flex machine (Applied Biosystems) with TaqMan™ Universal Master Mix and TaqMan Gene Expression Assays for human CCL2 (Hs00234140_m1) ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves discs were allowed to recover overnight in water and assayed in 20 µg/mL HRP and 6 µM L-012 in a 96-well luminometer plate (Thermo Fisher Scientific), with or without 100 nm flg22 ...
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bioRxiv - Developmental Biology 2022Quote: Mice were anesthetized using isoflurane and injected with 1% Biocytin-5(and-6-)- Tetramethylrhodamine (Biocytin-TMR: ThermoFisher Scientific; Waltham, MA, Cat# T12921). After 30 minutes in circulation ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Virus mutant libraries were rescued in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence in a T75 flask (75 cm2) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were washed three times and ProLong® Gold Antifade mounting reagent containing DAPI (4’,6-diamidino-2-phenylindole) (Thermo Scientific, #P10144) was applied to mount the coverslips.
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bioRxiv - Cancer Biology 2022Quote: ... 200’000 cells of selected monocolonies of either cell line were seeded in 6-well plates and transfected on next day with CRISPR plasmids using Lipofectamine 2000 (Invitrogen by Life Technologies #11668019) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: The olfactory epithelia were dissected from 6-week-old mice and stored the tissue at -80°C in RNAlater Solution (Invitrogen, Cat#: AM7020) until use ...
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bioRxiv - Bioengineering 2022Quote: ... IL USA) and solution was rapidly dispensed between two the syringes 6 times to mix before injection into scintillation vials (Thermo Fisher Scientific) or 3D printed (Taz 4 ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Biophysics 2022Quote: ... passage p<=6) were obtained from the Radboud Biobank and cultured in Minimum Essential Medium Eagle (α-MEM) (Invitrogen, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... quasi-stable HeLa cells were seeded in three separate wells of a 6-well plate (Fisher Scientific, product number 50-202-137) for each L1 construct using complete puromycin media with 1 μg/mL doxycycline ...
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bioRxiv - Neuroscience 2021Quote: ... The specificity of this antibody has been previously validated by us and others by Western Blot, IF and IHC analyses (5, 6).The Anti-NeuN mouse monoclonal antibody (ThermoFisher MA5-33103) was used for IF ...
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bioRxiv - Neuroscience 2020Quote: ... and added with 6 mL of ice-cold Hibernate E solution supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktails (# PI78443 Fisher scientific). The samples were gently homogenized (20 strokes ...
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bioRxiv - Immunology 2020Quote: Drug treated or siRNA transfected HBEC 5637 cells were collected from 6-well plates (containing 300,000 cells/well) using cell dissociation buffer (Life Technologies, Waltham, MA). The cells were lysed with RIPA lysis buffer supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermofisher ...
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bioRxiv - Neuroscience 2020Quote: ... All reactions from fixation to clearing were carried out in a well of 6 x 10 microwell minitray (Thermo Fisher Scientific #439225). Brains were washed in PBST (0.3% TritonX-100 in PBS ...
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bioRxiv - Molecular Biology 2020Quote: sg-mEX3 or sg-mEX5 and SpCas9-2A-GFP plasmids at 1:1 molar ratio were co-transfected into ~80% confluence OC-k1 cells in 6-well plate using Lipofectamine 3000 (Thermo Fisher Scientific). The cells were incubated for 48 hours and then submitted for fluorescence-activated cell sorting ...
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bioRxiv - Immunology 2020Quote: ... PSG3 5 plasmid was co-transfected into 293T cells along with various HIV envelope plasmids 6 using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific, #11668019) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Immunology 2020Quote: ... were mixed by adding 3 μL of each (150 pmol) and 6 μL (180 pmol) of TruCut Cas9 Protein v2 (Thermo Fisher Scientific) and incubated for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1ml of bone marrow was incubated in one well of 6-well plate with 3 ml StemPro™ MSC serum-free medium (Gibco, Thermo Scientific). Media was changed after every two days until it reached to confluency ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured in a 6-well plate in 1mL of media (DMEM, Thermofisher Cat# 12430054; 10% FBS, FisherSci Cat# 10437028; 1x Anti-Anti, Thermofisher Cat# 15240062) with 8ug of polybrene and 100uL of 10x pLVX-cOVA-Puro (Addgene Cat#135073 ...
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bioRxiv - Bioengineering 2021Quote: ... a gene fragment consisting of VH/CH1-(G4S)6 Linker-VH/CH1-CH2-CH3-(G4S)6 Linker-VH/CH1 DNA sequences was synthesized using GeneArt® gene synthesis services (ThermoFisher Scientific) and cloned into TMV viral backbone (Icon Genetics).
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bioRxiv - Microbiology 2021Quote: ... individual LC-MS experiments were conducted on 6 μL of each sample through 85 minutes of data acquisition on an Orbitrap Fusion (Thermo Fisher Scientific) mass spectrometer with an in-line Easy-nLC 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: VeroE6 cells (ATCC CRL-1586) were seeded at a density of 6*104/well onto cell culture 96-well plates (Nunc, Cat.#167008). Two days later ...
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bioRxiv - Microbiology 2021Quote: Vero E6 cells (ATCC CDL-1586) were grown to 80% confluence in 6-well tissue culture plates in DMEM (ThermoFisher Scientific/Gibco) containing 5% FBS (ThermoFisher Scientific/Gibco ...
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bioRxiv - Microbiology 2021Quote: ... then 50 μl of the EV preparations were mixed with 250 μl PBS staining buffer containing 2.5 µg/ml DAPI (4’, 6-diamidino-2-phenylindole; Thermo Fisher Scientific, USA)) and kept at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... 1:6 and 1:9 or 1:10 and 1:100) of ISF samples were prepared using OPTIMEM+ Glutamax-1 (Gibco #51985-026) and kept on ice until lipofection ...
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bioRxiv - Neuroscience 2022Quote: We dissected the olfactory epithelium from 6-week-old mice and stored the tissue at -80°C in RNAlater Solution (Invitrogen, Cat#: AM7020). We extracted RNA with the QIAGEN miRNeasy mini kit (Cat# ...
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bioRxiv - Developmental Biology 2022Quote: ... for 5 minutes at RT and then split with 1:8 ratio into 6 well plates pre-coated with 1:200 diluted geltrex in DMEM/F12 medium (Thermo Scientific A1413201) for 30 minutes at 37C ...
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bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected after 6 days and passed through a gravity chromatography column containing 1 ml of PierceTM Protein G Agarose resin (ThermoFisher Scientific 20397). The column was washed with 20 ml of wash buffer (150mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... cells grown to 80% confluency in 6-well or 10 cm plates were washed once in cell culture-grade PBS (Gibco, 10010-015), scrapped in ice-cold cell culture-grade PBS and collected at 300 g and 4°Cfor 5 min in a 1.5 ml microfuge tube ...
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bioRxiv - Developmental Biology 2022Quote: ... and the delta-delta-ct method was used for quantification (CFX maestro software, Bio-Rad or QuantStudio 6 Flex Real-Time PCR System software, Thermo Fisher).
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bioRxiv - Zoology 2022Quote: ... or 5,6,11-trideoxyTTX (10−5 M) together with a dextran conjugated with Alexa Fluor 555 (M.W. 10,000, 10−6 M; D34679, Thermo Fisher Scientific, MA, USA) for 5 min ...
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bioRxiv - Epidemiology 2020Quote: ... Real-time PCRs were performed using 6-carboxyfluorescein (FAM) and black hole quencher (BHQ1)-labeled TaqMan probes with TaqMan Gene Expression Master Mix (Applied Biosystems, USA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Measurements of MAO-B enzyme activity in tissue of 6-OHDA model was assessed using a commercially available kit (A12214, Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Seedlings were germinated on 0.5x Murashige & Skoog plates (0.5x MS, MSP01, Caisson Laboratories; 6% w/v Bioworld Phytoagar, 401000721, Fisher Scientific, pH 5.7) in a 150 µmol m−2 s−1 16 h light / 8 h dark regime at 24°C for three days.
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bioRxiv - Plant Biology 2019Quote: ... PCRs were carried out using the standard setting in a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific, Waltham, MA, U.S.A.). The mean cycle threshold (Ct ...
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bioRxiv - Developmental Biology 2019Quote: ... aliquots of 5 × 104 293FT cells were seeded onto poly-L-lysine (PLL)-coated 6-cm dishes and co-transfected with 2 μg of each pCAG-based expression vector using Lipofectamine 3000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Genetics 2020Quote: MEFs were fixed on 6-well glass bottom dishes with 4% PFA (diluted from 16% stock in PBS; ThermoFisher Scientific, Massachusetts, US), washed with PBS ...
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bioRxiv - Immunology 2020Quote: ... or aged (26-28 mo) female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco, 11995-073) supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Spag17PcdoTaqMan® Sample-to-SNP™ assays were performed according to manufacture instruction and run using QuantStudio 6 Real Time PCR machine (Applied Biosystems).
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bioRxiv - Zoology 2020Quote: ... each immunoblot lane represents a cell lysate produced from an entire well of a 6-well cell-culture dish (Thermo Scientific, Nunc). Lyates were diluted with SDS loading dye (final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated on either NuPAGE Novex 10% polyacrylamide Bis– Tris gels with 1XNuPAGE MOPS SDS buffer or 6% Tris-glycine gels with 1XTris-glycine running buffer (all Thermo Fisher Scientific), and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Synthetic Biology 2020Quote: Cell growth and multiplication was assessed by Fluorescence Assorted Cell Sorting and fluorescence microscopy with 6-Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE, Life Technologies, USA) as a probe ...