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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... IRDye 800LT goat anti-rabbit (Li-Cor, 925-32210) or Alexa Fluor 680 donkey anti-sheep IgG (H+L; Invitrogen), and visualized using the Li-Cor Odyssey Imaging System ...
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bioRxiv - Genomics 2019Quote: ... 1988) coupled to 10ul magnetic beads (Dynabeads™ M-280 Sheep Anti-Mouse IgG) and resuspened in 500ul TRIzol (Invitrogen) and stored at -80°C.
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bioRxiv - Genomics 2019Quote: ... and incubated with rotation overnight with antibody coupled to species-specific secondary beads (10 μg primary antibody as indicated coupled to 125 uL of Sheep anti-Rabbit or anti-Mouse Dynabeads; ThermoFisher). After incubation ...
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bioRxiv - Bioengineering 2020Quote: ... R&D systems sheep anti-human CD31/PECAM-1 antibody (AF806) and Hoechst 33324 (H3570) were obtained from Thermo Fisher Scientific (Whaltam ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ml of Dynal sheep-anti mouse IgG paramagnetic beads were non-covalently coupled with 60 µg of anti-His mAb (Invitrogen) in PBS plus 0.1% immunoglobulin-free BSA (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then incubated with the sheep anti-goat Alexa Fluor 594 (A11058, Life Technologies, Grand Island, NY, USA) and DAPI (D1306 ...
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bioRxiv - Neuroscience 2021Quote: ... Then slides were washed 3×5’ in TBS and incubated in donkey-α-sheep-488 (1:500, Life Technologies, A11015) in TBS for 90’ ...
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APOE ε4 gene dose effect on imaging and blood biomarkers of glial reactivity and β-amyloid pathologybioRxiv - Neuroscience 2022Quote: ... coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads and a KingFisher Flex instrument (Thermo Fisher Scientific), and further analyzed by liquid chromatography-tandem mass spectrometry (LC–MS/MS) ...
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bioRxiv - Cancer Biology 2022Quote: ... or normal IgG (1μg) was incubated with 50 μl Dynabeads M-280 sheep antimouse IgG magnetic beads (Thermo Fisher, 11202D) in 200ul wash buffer (50 mM pH 7.5 Tris-HCl ...
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bioRxiv - Pathology 2019Quote: ... Aliquot concentration was determined by enumerating colony growth on agar plates with 5% sheep blood (Remel, Thermo Fisher, Lenexa, KS) at the time of freezing ...
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bioRxiv - Microbiology 2019Quote: ... Any typical colony was streaked to a trypticase soy agar plate supplemented with 5% defibrinated sheep’s blood (Thermo Scientific-Remel) to confirm isolate purity ...
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bioRxiv - Physiology 2020Quote: ... brain sections were washed and incubated with anti-sheep Alexa Fluor® 488 (diluted 1:1,000 in 0.1M PB, A11015, Life technologies), and anti-rabbit Alexa Fluor® 488 (diluted 1:1,000 in 0.1M PB ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and incubated with anti-rat Ab magnetic beads at 1 bead/target cell for 40 min at 4C (Dynabeads sheep anti-rat IgG, Invitrogen). Cell suspensions were stained with Abs specific for congenic markers ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and incubated with anti-rat Ab magnetic beads at 1 bead/target cell for 40 min at 4C (Dynabeads sheep anti-rat IgG, Invitrogen). Cells were sorted into 3mL of complete media (RPMI 1640 ...
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies used were mouse anti-rabbit (1:10’000, Jackson, 211-032-171) and rabbit anti-sheep (1:10’000, Invitrogen, 81-8620).
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bioRxiv - Microbiology 2022Quote: ... Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922) were passaged twice on tryptic soy agar with 5% sheep’s blood (Thermo Scientific) at 37° C in 5% CO2 for 18-24 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... was grown overnight on blood agar plates (with 5% sheep blood) or in brain heart infusion broth (SA, ThermoFisher Scientific) until OD600=0.4 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS three times and incubated in a PBST solution containing the secondary antibody (Alexa Fluor 647-conjugated donkey anti-sheep, 1:1000, A21448, Invitrogen) and nuclear dye ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated with 1:200 donkey-anti-sheep (A11015, MoBiTec) and 1:1000 donkey-anti-rabbit (A21206; Invitrogen) secondary antibodies for 1 h at RT and washed 3 times ...
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bioRxiv - Microbiology 2024Quote: Additional homogenised tumour tissues from four patients (PT09, PT14, PT15, PT17) were also cultured on sheep blood agar plates (Thermofisher) in an anaerobic hood at 37°C condition ...
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bioRxiv - Developmental Biology 2024Quote: ... blocking with 5% heat-inactivated sheep serum and incubation with an anti-digoxigenin antibody conjugated to alkaline phosphatase (Invitrogen, AM1326). NBT/BCIP (4-nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolylphosphate ...
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bioRxiv - Genetics 2023Quote: ... Samples were incubated for 2 hours at 4°C with rotation before adding 50 µL sheep anti-mouse IgG dynabeads washed in IPP buffer (Invitrogen). The unbound fraction was retained ...
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bioRxiv - Microbiology 2023Quote: ... serial dilutions of the cryopreserved homogenates were plated on eight different media: CBA (Columbia Blood Agar supplemented with 5 % defibrinated sheep blood (Thermofisher)) ...
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bioRxiv - Microbiology 2023Quote: ... Afterwards the blots were incubated (1:5000 dilution) with a sheep anti-mouse horseradish peroxidase-conjugated antibody (Life Technologies, France). The proteins were revealed using an ECL Western Blotting Substrate (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were placed in the secondary antibody solution containing 1xPBS/0.1% Triton X-100/3% donkey serum and a 1:1000 dilution of donkey anti-sheep 647 (A-21448, Thermofisher) for 10 days.
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies were used at 1 μg mL-1 for goat anti-rabbit and sheep anti-mouse linked horseradish peroxidase (Invitrogen). Blots were developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce).
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bioRxiv - Microbiology 2023Quote: ... for 16 h at 4°C and with a secondary fluorescent antibody (Alexa-488 anti-sheep diluted 1:50,000; Thermo Fisher) for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The depletion of endogenous microbiota was monitored by conducting quantitative fecal cultures on Sheep Blood agar plates (Thermo Scientific, #R01200). This allowed the assessing of the reduction in microbial colony-forming units (CFUs ...
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bioRxiv - Microbiology 2023Quote: ... The culturing for all the samples was done onto tryptic soy agar with 5% sheep’s blood (Thermo Fisher Scientific, USA). The plates were incubated at +35±2°C in 5% CO2 and read at 24-hour intervals for four days ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was sheared using a Bioruptor sonicator for four sets of 15 × 30s at amplitude of 50% with 30s shut off intervals and immunoprecipitated using sheep anti- mouse conjugated Dynabeads (Invitrogen) coupled with 1:10 mouse anti MYC antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... primary antibodies targeting SARS-CoV-2 proteins were used at indicated dilutions / concentrations (Table 1, MRC PPU Dundee) before staining with Donkey anti-Sheep AF488 Secondary Antibody (Invitrogen) at 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then washed and incubated in secondary antibody (donkey anti-rabbit Alexafluor 488, Invitrogen, Carlsbad, CA A-21206 1:500; donkey anti-sheep Alexafluor 647, Invitrogen, A-21448 1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-MRC2 (as above) as a primary antibody with a secondary of Donkey anti-sheep conjugated to a fluorophore (Invitrogen). Cells were then analyzed and/or sorted on one of the above devices ...
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bioRxiv - Immunology 2023Quote: ... and either goat anti-rabbit IgG-AlexaFluor 647 (A-21245) or donkey anti-sheep IgG-AlexaFluor 647 (A-21448, all from ThermoFisher). Cells were washed twice with 0.1% Tween20 in PBS and once with PBS containing DAPI to visualize the nucleus ...
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bioRxiv - Microbiology 2023Quote: ... Cells stained with anti-ACE2 and anti-GD3 were further incubated with secondary antibodies Alexa Fluor 488-labeled sheep anti-goat and goat anti-mouse (Invitrogen) for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... and immediate collection of bead bound RNA and transcription complexes in Trizol following immunoprecipitation by Rpb1 CTD (4H8) mouse mAB conjugated to Dynabeads M-280 sheep anti-mouse IgG (ThermoFisher). mNET-seq experiments were completed in biological replicate ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg/µL of anti-Rpb3 or anti-Rpb4 antibody was incubated with 25 µL of magnetic beads (Dynabeads M-280 Sheep anti-Mouse IgG, 11202D, Invitrogen) for 1 h at 4°C in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cultures were plated on THM-blood-agar consisting of 5% of sheep blood (Remel Lenexa, KS) in THM with 1.5% agar (Fisher Scientific). The bacteria were routinely cultured in an anaerobic chamber in an atmosphere consisting of 80% N2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This step was followed by incubation with 50μl of Dynabeads® M-280 sheep anti-mouse IgG (11201D, Thermo Scientific) for 3 hours ...
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bioRxiv - Immunology 2024Quote: ... Cells were then isolated as CD4 or CD8 respectively with immunomagnetic beads (Dynabeads Mouse CD4 +, Invitrogen, catalog: 11445D; or Dynabeads Sheep anti-Rat IgG, Invitrogen, catalog ...
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bioRxiv - Molecular Biology 2024Quote: ... We followed the kit protocol except for conjugating 5µg of anti-total RNAPII to sheep anti-mouse dynabeads (Life Technologies). Sequencing libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina.
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bioRxiv - Molecular Biology 2024Quote: ... we prepared for each sample and IgG control: 120□μl of Dynabeads M-280 Sheep anti-mouse IgG (Thermo Fisher) or 40□μl of Dynabeads protein G (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... Cryosections were incubated for 1h in secondary antibody (1:1000 donkey anti-sheep conjugated with AlexaFluor-488; ThermoFisher Scientific (Invitrogen)) ...
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bioRxiv - Genomics 2024Quote: ... Cryosections were incubated for 1h in secondary antibody (1:1000 donkey anti-sheep conjugated with AlexaFluor-488; ThermoFisher Scientific (Invitrogen)) ...
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bioRxiv - Cell Biology 2020Quote: S1-modETV2 protocol (4 days) – h-iPSCs were dissociated into single cells with TrypLE select (ThermoFisher, Cat No. 12563-029) and plated on Matrigel at a density of 50,000 cells/cm2 in mTeSR1 medium with 10 μM Y27632 ...
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bioRxiv - Developmental Biology 2020Quote: ... Both constructs were amplified with primers (Table S1) containing attB for Gateway cloning into pDONR221 using BP Clonase II (Invitrogen). pDONR221 clones were verified by sanger sequencing (eurofins) ...
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bioRxiv - Developmental Biology 2020Quote: ... The gene sequence of TTL3 gDNA was amplified (primers TTL3-gDNA-LP and TTL3-gDNA-BP, Table S1) and subcloned into the pDONR221 (Invitrogen). GFP CDS was cloned into the pEN-R2-F-L3.0 vector (Karimi et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... was amplified (primers pTTL3-LP and pTTL3-BP, Table S1) and subcloned into the pENTR5’-TOPO TA Cloning vector (Invitrogen). The gene sequence of TTL3 gDNA was amplified (primers TTL3-gDNA-LP and TTL3-gDNA-BP ...
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bioRxiv - Genomics 2020Quote: ... the DNA from above was incubated with 1 μl (5 U/μl) of single-strand specific S1 nuclease (ThermoFisher Scientific) in 1x reaction buffer (40 mM sodium acetate ...