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Citations for Thermo Fisher :
3851 - 3900 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... was synthesized by Genscript and cloned into a modified pMT/BiP insect cell expression plasmid (Invitrogen) designated pT350 ...
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bioRxiv - Cell Biology 2024Quote: Cells were transiently transfected with required plasmids using LTX transfection reagent (Invitrogen) or immunostained with required antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... in addition to the Mir plasmids described above (Invitrogen Block it kit) were pCAGGS-GFP (gift from S ...
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bioRxiv - Biochemistry 2023Quote: ... were transfected with 200 ng psDARPin-expressing plasmids using lipofectamine 3000 (Invitrogen), following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... Each plasmid was linearised using restriction enzymes (XbaI + KpnI, Thermo Scientific FastDigest), and 10 µg of DNA was used for each transformation ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... We performed standard curves using serial dilutions of plasmids (TOPO pCR2.1 (Invitrogen)) containing the target sequence (108-103 copies per 3 μl) ...
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bioRxiv - Biochemistry 2024Quote: All plasmids were transfected in HEK-293 cells with Polyethyleneimine (PEI) (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The amplicons were further cloned in pJET1.2 cloning plasmid (ThermoFisher Scientific, USA), and the recombinant plasmids were then transformed into XL1 blue Escherichia coli competent cells ...
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bioRxiv - Microbiology 2023Quote: ... to derive 12 clones using the GeneJet plasmid purification kit (Thermo Scientific) for Sanger sequencing.
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bioRxiv - Neuroscience 2022Quote: Expression plasmids for AAV production were generated by gene synthesis (GeneArt, ThermoFisher) and subcloned into the pAAV plasmid (AAV Helper-Free System ...
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bioRxiv - Immunology 2022Quote: ... Each plasmid was transformed into One Shot Stbl3 chemically competent cells (Invitrogen) and purified by ZymoPURE plasmid Maxiprep kit (Zymo research) ...
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bioRxiv - Microbiology 2023Quote: ... Endonuclease free plasmid preparations were made using Midi Prep kit (Thermo Fisher). For lentivirus production HEK293T cells were seeded at 1×105 cells/well density in a 6-well plate and grown for 72 h until 80-90% confluence ...
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bioRxiv - Microbiology 2023Quote: ... fragments for fungal transformation were fused into pENTR4 plasmids (Thermo Fisher Scientific) by HiFi DNA assembly (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... The purified plasmid was linearized by Anza 24 MssI restriction enzyme (Invitrogen), purified again ...
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bioRxiv - Cell Biology 2023Quote: ... The final plasmid construct was generated by MultiSite Gateway® cloning (Invitrogen). The strain expressing HIS-1/H4-GFP was made using the MosSCI technique (42) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were transiently transfected with the indicated plasmids using LipofectAMINE 2000 (ThermoFisher) according to the manufacturer’s protocol and cultured on poly-lysin coated glass cover-slides ...
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bioRxiv - Genetics 2023Quote: ... Confirmed plasmids were transfected into HEK293T cells with lipofectamine 3000 (Invitrogen, L3000001) following the manufacturer’s protocol.
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bioRxiv - Biophysics 2023Quote: ... 0.5 µg of hybrid transposase plasmid and Lipofectamine 2000 transfection reagent (Invitrogen) with a 1:2.5 DNA to transfection reagent ratio.
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bioRxiv - Neuroscience 2023Quote: ... were transfected with TDP-43 or control plasmids using Lipofectamine 2000 (Invitrogen) for 48hr following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was isolated from positive clones (GeneJet Plasmid Miniprep Kit, Thermo scientific) and subjected to Sanger sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmid transfections were performed using Lipofectamine 3000 transfection reagent (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: Plasmid transfection was performed using the Neon Transfection System (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... with 3 μg of plasmid DNA in 400 μl Opti-MEM (Gibco), and added to each dish ...
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bioRxiv - Microbiology 2023Quote: ... and then transfected with 3µg of amplicon plasmid using Lipofectamine 2000 (Invitrogen). 24 hours after transfection ...
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bioRxiv - Immunology 2023Quote: ... Transfer plasmid and packaging vector were mixed with Opti-MEM (ThermoFisher Scientific). In a separate tube ...
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bioRxiv - Genetics 2023Quote: ... or PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit (Thermo Fisher Scientific), both which remove bacterial endotoxins ...
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bioRxiv - Evolutionary Biology 2023Quote: ... plated out and purified with the GeneJET Plasmid Miniprep Kit (Thermo Fisher). To validate the correct inserts ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Transfection-ready plasmids were purified from bacterial cultures by maxiprep (Invitrogen, #K20006). Expi293 HEK cells (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmids were transfected into HeLa cells using TurboFect Transfection Reagent (Invitrogen). After 48h ...
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bioRxiv - Developmental Biology 2023Quote: Rescue Plasmids were transfected into relevant cell lines using Lipofectamine 2000 (Thermofisher). Cells with insertions were selected using Puromycin (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... and the constructed transfer plasmid using the Cellfectin II (Thermo Fisher, 10362100) transfection reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... pPAX2 and VSVg plasmids using Lipofectamine® 2000 reagent (Thermo Fisher Scientific) in Opti-MEM™ (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... RPE1 cells were transfected with this plasmid by using Lipofectamine 3000 (Invitrogen). Transfected cells were subjected to puromycin selection after transfection and subsequently seeded as single cells into 96-multiwell plates in order to screen for CEP290 KO clones ...
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bioRxiv - Cell Biology 2023Quote: Bacterial expression plasmids were generated via Gateway cloning (Invitrogen, Carlsbad, CA, USA). The effector sequences of SopB ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and subcloned into pFastBac1 recombination plasmid (Thermo Fisher Scientific, Waltham, MA, US) between BamHI and HindIII restriction sites ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmid pZA31-mCherry-TnpB was designed using Vector NTI software (Life Technologies) and synthesized de novo by GENEWIZ Gene Synthesis Services (GENEWIZ ...
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bioRxiv - Neuroscience 2023Quote: ... transfection of the scFv expression plasmids was carried out using 293fectin (ThermoFisher) following vendor’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... with 1µg of Plasmid DNA in 30µl of Opti- MEM medium (ThermoFisher). The mix was added to cultured neurons for 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: T7 promoter-containing plasmid DNA was digested with FastDigest MvaI (Thermo Scientific) and used as template for in vitro transcription of tRNA using the AmpliScribe T7-Flash Transcription Kit for 4 h at 42°C (see Table S3) ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids at 4:1 ratio (by mass) using Lipofectamine 2000 (Invitrogen, 11668019) in Opti-MEM (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... Synthetic genes were ordered from GeneArt and plasmids purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products and plasmids were restricted with FastDigest BshTI (Thermo Fisher, FD1464) and NotI (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... and then transfected with 3µg of amplicon plasmid using Lipofectamine 2000 (Invitrogen). 24 hours after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and SARS-CoV-2 Spike plasmids using Lipofectamine 3000 (Thermo Fisher, L3000015). 48 to 72 hours post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng pTI-ARE-LUC reporter plasmid in 10 µL OptiMEM (Gibco) was transected into each well using Fugene HD at a dilution of 1 µg plasmid DNA:4 µL Fugene ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments were cloned into the pENTR/D-TOPO plasmid vector (Invitrogen) to obtain pYC19 (pENTR-AGB1.1 ...
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bioRxiv - Neuroscience 2024Quote: ... All electroporated plasmids were purified with an endotoxin-free maxi kit (Invitrogen) and dissolved in sterile PBS 1X containing 0.025% Fast Green (SIGMA) ...
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bioRxiv - Genetics 2024Quote: ... or PureLinkTM HiPure Plasmid filter Maxiprep Kit (Thermo Scientific, Lafayette, CO, USA). Natural transformation was performed according to the procedure described in (27).
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bioRxiv - Neuroscience 2024Quote: ... plasmid DNA (diluted in Opti-MEM, GIBCO, Thermo Fisher Scientific, Waltham, MA) was mixed with 1.5ul of PLUS™ Reagent and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... plasmid DNA (diluted in Opti-MEM, GIBCO, Thermo Fisher Scientific, Waltham, MA) was mixed with 1.5ul of PLUS™ Reagent and incubated for 5 min at room temperature (RT) ...