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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... plasmid (EV or HLA-A2/Kb; 1 µg/µL) complex at 2:1 ratio prepared in Opti-MEM (Gibco, UK) for 18 hours in the presence or absence of 100 ng/mL type I IFNα (PBL Assay Science ...
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bioRxiv - Bioengineering 2019Quote: ... via covalent attachment to COOH groups on the particles via standard EDC chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Thermo Fisher Scientific, MA) and N-hydroxysulfosuccinimide sodium salt (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were washed in 3 times in 3% PBST (10 min) and incubated in goat anti-chicken Alexa Fluor 488 (Invitrogen, A11039, 1:1000) in blocking buffer at room temperature for 2hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16°C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3).
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Bioengineering 2023Quote: Passage 3 and passage 4 cells from 8 donors (4 male and 4 female) were expanded in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) and were cultured until passage 5 (p3-5 and p4-5 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded in microglia differentiation medium with 3 μM Fluo-4 AM and 3 μM Fura-Red AM (Molecular Probes) in the presence of Pluronic Acid F-127 (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: ... Blocks of tissue of ~3×3×4 cm (depth×width×height) were cut and prepared for sectioning using a Vibrating Blade Microtome (Thermo Fisher) at a thickness of 500μm ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified with Agencourt AMPure XP beads in 0.7:1 ratio (beads to PCR reaction volume) and quantified with the Quant-iT™ High-Sensitivity dsDNA Assay Kit (Invitrogen). The amplicons were pooled together and sequenced on the Illumina HiSeq 2500 using HiSeq Rapid SBS Kit v2 (500 cycles ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures on feeder layers were performed on Mitomycin C-treated MEF as described above with passaging every 7 days using 1 mg/mL collagenase IV in DMEM/F12 (Life technologies) the feeder-free culture was performed on Geltrex™ (Life technologies ...
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bioRxiv - Immunology 2019Quote: ... Then to stain the cells we used antibodies against LFA-1 (clone I21/7) and 4G10 with Alexa Fluor 568–conjugated phalloidin (Life Technologies) and DAPI ...
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bioRxiv - Biochemistry 2019Quote: ... The valve then switches to engage the passive split such that a majority of LC effluent is diverted to waste prior to the third dimension PC. 13 RP-SAX-RP fractions were analyzed. Either an LTQ Velos (Figs. 1, 7) or Q-Exactive HF (Thermo Scientific) machine was used to acquire MS/MS spectra in data-dependent mode as described (Zhang et al. ...
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bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
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bioRxiv - Neuroscience 2020Quote: Adrenal glands and brain extracts were collected from P0-1 Syt-7 WT and KO mice and lysed in RIPA buffer supplemented with Protease Inhibitor Cocktail (Invitrogen, 89900). The supernatants were collected and protein concentrations were estimated by using the BCA Protein Assay Kit (Pierce 23227 ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2022Quote: 90μL of paraformaldehyde fixed cells was incubated with 5 x 10-7 M DAPI and 1:100 Vybrant™ DiD Cell-Labeling Solution (Invitrogen) at 37C for 20 minutes ...
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bioRxiv - Immunology 2019Quote: ... and frozen down in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Frozen PBMCs were stored at −80°C before shipping on dry ice to Benaroya Research Institute for further processing and stimulation as described above.
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bioRxiv - Immunology 2019Quote: ... one half being used immediately and the other half frozen in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Freshly isolated PBMCs were plated in 96 deep-well plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was retrotranscribed and amplified in an QuantStudio 7 Flex thermocycler (Applied Biosystem) using Taqman RNA-to-Ct 1-Step Kit (Thermofisher, 4392653). The Taqman probes used were the following Jag1 (Mm00496902_m1) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 7 Flex). Primers used for qPCR reactions are listed in Supplementary Table S5 ...
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with solution containing 468 μl of 50 mM ammonium bicarbonate and 7 μg of Trypsin (Thermo Fisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR program comprised an initial denaturation for 30 s at 95 °C and amplification by 40 cycles of 15 s at 95 °C and 1 min at 60 °C and was ran in an ABI QuantStudio 7 Real Time PCR machine (Applied Biosystems). Expression values of DIN6 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were reverse-transfected into an arrayed 384- well plate library containing small interfering RNA (siRNA) or miRIDIAN microRNA mimic constructs (Horizon Discovery, Supplementary Tables 1-7) using Opti-MEM (Gibco 31985070) and Lipofectamine RNAiMAX transfection reagent (Invitrogen 13778150) ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 mM sodium citrate pH 5.0 buffer containing 7 M urea and 10.5 mM EDTA with 1 U RNase T1 (ThermoFisher Scientific, Waltham, MA) at 55°C for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... either Alexa Fluor568- or Alexa Fluor647-conjugated antibody in 3 % BSA/PBS (Invitrogen, 1:500). Following three PBS washes ...
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bioRxiv - Microbiology 2019Quote: ... membranes were blocked with 3% BSA and incubated with Streptavidin-HRP (1:4000; Thermo Scientific) in 0.3% BSA ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Sections were washed by PBTriton 3 times and incubated with 1:1000 phalloidin–Alexa488 (Invitrogen) and 1:4000 DAPI in PBTriton for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... AsPC-1 and BxPC-3 cells were maintained in Roswell Park Memorial Institute (RPMI, Invitrogen) supplemented with 10% FBS and 4 mM L-glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... PerCP-eFluor 710 mouse anti-human CD223 (LAG-3) (1:100; 46-2239-41, Invitrogen), and BV421 mouse anti-human CD366 (TIM-3 ...
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bioRxiv - Cell Biology 2021Quote: ... DNA in adult testes was stained with To-Pro-3 (1:1000, Life Technologies, T3605) solution in the dark for 15min ...
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bioRxiv - Biochemistry 2022Quote: Cells were either transfected with PEI (1 μg/3 μg DNA) or lipofectamine 2000 (ThermoFisher) diluted in OptiMEM (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated for 3 min with Hoechst 33342 (Thermo Fisher Scientific, dilution 1/10,000) for live-cell nuclear fluorescence labeling.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were counted every 1-3 days using CyQuant Direct Proliferation Assay kit (Life technologies). An inverted plate reader (Flexstation 3 ...
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bioRxiv - Genetics 2020Quote: ... 3-Phosphoglycerate kinase (Pgk1) protein was detected with anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) and HSP90 protein was detected with a mouse anti-HSP90 monoclonal antibody (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with a 3:1 mixture of Newborn Calf Serum (NBCS) (Gibco, Thermo Fisher Scientific) and Fetal Calf Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with a 3:1 mixture of Newborn Calf Serum (NBCS) (Gibco, Thermo Fisher Scientific) and Fetal Calf Serum (FBS ...
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bioRxiv - Pathology 2020Quote: ... It consisted of the DMEM medium and F12 in ratio 1:3 (Gibco Life Technologies), supplemented with 2.5 ml FBS ...
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bioRxiv - Pathology 2020Quote: ... It consisted of the DMEM medium and F12 in ratio 1:3 (Gibco Life Technologies), supplemented with 2.5 ml FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were washed 3 times and incubated with goat-anti-rabbit-Alexa594 (Invitrogen; 1:1000) plus goat-anti-mouse-Alexa488 (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... the B27 supplement in 3:1 media was replaced by N2 supplement (Gibco, #17502‒048) and retinoic acid was reduced to 5 µM.
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bioRxiv - Genetics 2020Quote: ... 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Zoology 2021Quote: ... and 3) an Alexa Fluor® 488-conjugated streptavidin (S11223, Thermo Fisher Scientific; 1:500) for 2 h at 28°C ...
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bioRxiv - Developmental Biology 2021Quote: ... the blastomeres were stained with 1 µM TO-PRO-3 iodide (Thermo Fisher Scientific, T3605) for 30 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... at 1:2000 and a nuclear counterstain (TO-PRO™-3 Iodide ThermoFisher Scientific T3605) at 1:2000 was performed for 2 hours before 3x 15 minute PBS-T washes and a final PBS-T wash overnight at 4°C while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were washed 3 times in PBS then incubated in 1:40 phalloidin (Thermo Fisher) in PBS for 20 minutes at room temperature in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... 10 nl of 1 mM FluoZin-3 (FZ3, 847 Da, Thermo Fisher Scientific, Cat. F24194), 100 mM CaCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were rinsed 3 times with 1 mL of 1X PBS (Gibco, 10010-023), quenched in 1 mL of 0.1% sodium borohydride/PBS solution (Sigma ...
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bioRxiv - Genetics 2022Quote: ... TO-PRO-3 Iodide carbocyanine monomer nucleic acid stain (1:1000, Thermo Fisher, cat# T3605) was used to stain DNA ...
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bioRxiv - Plant Biology 2022Quote: ... in a ratio of 3:1 (FuGENE:DNA) and 65 μl of Opti-MEM (Thermo Fisher) was added ...
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bioRxiv - Microbiology 2022Quote: ... or 1-3 µL of PageRuler Prestained Protein Ladder (10-180 kDa) (Thermo Scientific, 26617) were used as a ladder ...