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Citations for Thermo Fisher :
3701 - 3750 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cloning of the plasmids was carried out using restriction enzymes (Thermo Fisher), PfuI polymerase (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... were transfected with pMSCV and pCl-Eco plasmids using Lipofectamine 3000 (Invitrogen) and cultured for 3 days ...
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bioRxiv - Cancer Biology 2020Quote: Cells were first transfected with plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1200ng of Fibcd1 plasmid was mixed with 150µl Opti-Mem (Gibco #31985062), 700ng pGag/pol ...
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bioRxiv - Neuroscience 2019Quote: ... A LoxP-flanked EF1α-Puro-Tk cassette in a pENTR plasmid (Invitrogen) was inserted between the two homology arms ∼100bp upstream of the repeat region and in between the two Nsi1 sites ...
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bioRxiv - Cell Biology 2021Quote: ... with 0.2 μg plasmid (total) and 0.5 μl lipofectamine 2000 (Life technologies) and seeded in the centre of a 25-mm poly-L-lysine-coated coverslip ...
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bioRxiv - Microbiology 2020Quote: The plasmids were transiently transfected into the cells using Lipofectamine 3000 (Invitrogen) or Polyethylenimine ‘Max’ (Polysciences ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant plasmid was linearized using the Ncol restriction enzyme (ThermoFisher Scientific) and the MEGAscript® T7 Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and then cloned into the adenoviral plasmid pAd/CMV/V5-DEST (Invitrogen) using LR ClonaseTM II Enzyme Mix (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were transfected in a 1:2 ratio with Lipofectamine 2000 (ThermoFisher).
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bioRxiv - Physiology 2021Quote: ... template plasmid DNA was removed by FastDigest DpnI digestion (Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Microbiology 2019Quote: ... to make the plasmid pRG-PBG98-B-GFP11 (GeneArt, ThermoFisher Scientific, UK). DF-1 cells were co-transfected with this plasmid and pRG-PBG98-A to generate the recombinant virus PBG98-VP1-GFP11 ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with the indicated plasmids using Lipofectamine 2000 (Invitrogen) as per the manufacturer's instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was transfected in ExpiCHO-S cells (Gibco-Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was transfected in ExpiCHO-S cells (Gibco-Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... solution 1 was prepared by diluting plasmid into Opti-MEM (ThermoFisher Scientific). Solution 2 was prepared by diluting PEI (1 µg/µl ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection-grade plasmid DNA were isolated using a PureLink Maxiprep Kit (ThermoFisher).
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bioRxiv - Biophysics 2020Quote: ORF1 expression plasmids were transformed into Escherichia coli BL21STAR(DE3) cells (Invitrogen). One-liter cultures supplemented with 50 μg/mL kanamycin were inoculated with 20 mL of an over-night culture and grown at 37°C to an OD600 between 0.5 and 0.8 ...
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bioRxiv - Biophysics 2020Quote: ... and/or D3cpV plasmid DNA was transiently transfected using Lipofectamine 2000 (Invitrogen) mixed with OPTI-Mem (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid DNA was transfected using Lipofectamine LTX with plus reagent (ThermoFisher) or PEI (Polyetheleneimine ...
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bioRxiv - Cell Biology 2020Quote: ... or PureLink™ HiPure Plasmid Miniprep Kit (Art. No. K210011, Invitrogen™), eluted in sterile water and confirmed by enzymatic digestion and sequencing.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HEK293 cells using either Lipofectamine 2000 reagent (Invitrogen) or calcium phosphate transfection.
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bioRxiv - Biochemistry 2022Quote: ... These entry plasmids were transposed to the destination vector (pDEST8, Invitrogen, USA) with Gateway LR reaction according to the manufacture’s instruction for construct transfer plasmids ...
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bioRxiv - Neuroscience 2022Quote: ... Products were purified using the GeneJet Plasmid Mini Prep Kit (Thermo scientific).
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA was transformed into TOP10 chemically competent cells (Invitrogen, c4040-10) by the heat-shock method ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids were co-transfected into cells using Lipofectamine 3000 reagent (Thermo Fisher). The cell medium was replaced with fresh DMEM medium (containing 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... by cutting the plasmids containing the ATS variant with PmeI (ThermoFisher Scientific), which introduces two cuts and exposes homology regions to the MET15 locus ...
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bioRxiv - Biochemistry 2022Quote: ... The PLP/DM20 plasmid was transformed into DH10Bac strain (Thermo Fisher Scientific) for bacmid production ...
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bioRxiv - Molecular Biology 2022Quote: ... coli DH5α cells using a GeneJET Plasmid Miniprep kit (ThermoFisher Scientific K0503). Plasmids were generated by standard restriction digests ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2.5 µg pVSV-G plasmid with 10 µL Lipofectamine 2000 (Invitrogen) diluted in 4 mL Opti-MEM ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid concentrations were determined using a Quant-iT PicoGreen dsDNA kit (Invitrogen), and 200 ng of each plasmid was electroporated into 2 ODU of L ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were cloned into pJET1.2/blunt plasmid (Thermo Fisher Scientific) and transformed into NEB 5-alpha Competent Escherichia coli (High Efficiency ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μg plasmid was mixed with 1.5 μl Lipofectamine 3000 (Life Technologies) and 2 μl P3000 reagent (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing the CrPV or the minigenomes were linearized with Ecl136ii (Thermofisher) or Eco53KI (NEB) ...
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bioRxiv - Immunology 2022Quote: ... and the plasmid was recirculated by T4 DNA ligase (Thermo Fisher Scientific). The CAR constructs were transfected to Plat-A packaging cell line via Lipofectamine 3000 reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Five micrograms of linearised plasmid template were pre-treated with RNAsecure (ThermoFisher) and used in a 50μl transcription reaction containing 5 x transcription buffer (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmids were transformed into Escherichia coli BL21(DE3)pLysS cells (Invitrogen), and cells harboring the plasmids were grown at 37°C until the OD600 reached 0.7-1.0 in Luria Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: ... coli by the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA) and modified or used for full-length genome assembly.
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bioRxiv - Microbiology 2022Quote: ... coli using the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA). Table S3 lists the primers used for the construction of the variant S DNA fragments.
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were cloned and propagated in Mach I cells (Thermo Fisher Scientific) with the exception of pHelper ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The plasmids were purified by a PureLink miniprep kit (Thermo Fisher Scientific) and outsourced for Sanger sequencing (HyLabs ...
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bioRxiv - Plant Biology 2022Quote: ... The bait and prey plasmids were transformed into yeast strain MaV203 (Invitrogen): MATα leu2-3,112 trp1-901 his3Δ200 ...
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bioRxiv - Cancer Biology 2022Quote: ... Co-transfection of the plasmids was then performed using Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: PX459-hRABL3 plasmid was transfected into RPE1 cells using Lipofectamine 2000 (Invitrogen). After 24 hrs ...
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bioRxiv - Microbiology 2021Quote: ... 10 μg of each CHIKV plasmid was linearized overnight using NotI (Invitrogen), purified by phenol:chloroform extraction and used as a template for in vitro transcription using the SP6 mMessage mMachine kit (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmids were transformed into Agrobacterium tumefaciens GV3201 (Thermo Scientific, Waltham, MA, US) and selected using 50 mg/L spectinomycin and 50 mg/L gentamycin to maintain the pMP90 plasmid (MP Biomedicals Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... The ATRX plasmids were propagated using ElectroMAXTM Stbl4TM Competent cells (Life Technologies). To generate Flag-tagged DAXX expression plasmid (pCDH-Flag-DAXX-Puro) ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA transfection was performed with the LT-X transfection reagent (Invitrogen). Transfected cells were plated on poly-D-lysine (Sigma-Aldrich)−coated borosilicate cover glass (Paul Marienfeld ...
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bioRxiv - Genetics 2021Quote: ... The plasmids were used to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...