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Citations for Thermo Fisher :
3651 - 3700 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the cells were incubated with Alexa-488 goat anti-rat antibodies at 1:200 (Thermo Fisher Scientific, Cat#A11006) diluted in DPBS with 2% BSA for 1 h ...
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bioRxiv - Immunology 2023Quote: Mice were intraperitoneally (i.p.) injected with 200 μg of rat anti-mouse CD4 monoclonal antibody (Clone GK1.5; ThermoFisher Scientific) 1 day prior to RICs immunization ...
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bioRxiv - Immunology 2023Quote: ... rat anti-mouse CD45.2 (conjugated with APC, clone#104, cat#17-0454-82, Thermo Fisher Scientific, Inc, Waltham, MA) and rat anti-mouse CD90.2 (conjugated with FITC ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:200 in 1% BSA in PBS for 1.5 hours at room temperature and secondary antibody anti-rat Alexa Fluor 647 (Invitrogen) at a dilution of 1:1000 in 1% BSA in PBS for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... and pieces incubated in secondary antibodies [anti-rat secondary antibody (H+L) 1:400 (Thermo Scientific, Alexa Fluor 488), anti-rabbit 568 secondary antibody (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Ki67 (1:500, rabbit polyclonal, ab15580 Abcam or 1:500, rat monoclonal, 14-5698-82 Thermo Fisher Scientific); anti-RFP (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Stretched rat bladders were pinned out on rubber mats and treated with 2.5mg/ml of Dispase (Gibco-ThermoFisher Scientific) dissolved in Minimal Essential Medium (Gibco-ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Stretched rat bladders were pinned out on rubber mats and treated with 2.5mg/ml of Dispase (Gibco-ThermoFisher Scientific) dissolved in Minimal Essential Medium (Gibco-ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS before adding Donkey anti-Rat Alexa Fluor 488 secondary antibody (1:1000; Invitrogen; A21208) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... then with anti-rabbit IgG-Alexa Fluor 488 or anti-rat IgG-Alexa Fluor 594 (1:1000, Invitrogen, USA) for 2 h at room temperature (RT) ...
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bioRxiv - Systems Biology 2024Quote: Cortices of adult rats (Sprague–Dawley) were dissected and a synaptosome preparation was generated using Syn-PER (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-Mouse 568, Donkey anti-Rabbit 657, Donkey anti-Rat 488, Donkey anti-Goat 568; all at 1:1,000; Invitrogen) for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2020Quote: Plasmids were extracted from bacterial cultures with a Midi Prep kit (Invitrogen). One ug/ul of each plasmid was introduced into Arabidopsis protoplasts derived from four-weeks old plants by polyethyene glycol-mediated transfection and incubated overnight at room temperature (Fujikura ...
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bioRxiv - Cancer Biology 2021Quote: Plasmid transfection was carried out using Lipofectamine™ 3000 (Invitrogen™ 11668019) or ViaFect™ (Promega™ E4981 ...
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bioRxiv - Cancer Biology 2021Quote: Cell transfection was performed with plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: All plasmids were transfected with Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific) following manufacturer’s instructions ...
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Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotencybioRxiv - Developmental Biology 2021Quote: ... the plasmid underwent in vitro transcription using mMESSEAGE mMACHINE T7 kit (Invitrogen) and were purified by LiCl precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... 1-2 μg of Plasmid DNA along with transfection agents Lipofectamine (Invitrogen) or Plyetheleneimoine ...
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bioRxiv - Cell Biology 2019Quote: ... cloning the PCR amplicons in the pCR2.1 TOPO plasmid (Thermo Fisher Scientific) and transforming 10-beta competent E ...
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bioRxiv - Cell Biology 2020Quote: Transfection of plasmids was performed using Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 3.75 µg of each plasmid and 3.75 µl PLUS reagent (Invitrogen 15338100) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... coli and extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The construction was verified using enzymatic digestion with KpnI and PvuI.
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bioRxiv - Evolutionary Biology 2021Quote: ... coli cultures using PureLink HiPure Plasmid Midiprep Kit (Invitrogen, Cat. No. K210005). To facilitate DNA delivery to ESCs through nucleofection ...
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bioRxiv - Plant Biology 2022Quote: ... the linearized plasmid was digested with 20 U DNase I (Thermo Scientific) for 10 min at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmid assembly was performed by ligation with T4 DNA ligase (Thermo Scientific) or by the Gibson reaction using the NEBuilder HiFi assembly master mix (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... and 2.5 μg pVSV-G plasmid with 10 μL Lipofectamine 2000 (Invitrogen) diluted in 4 mL Opti-MEM ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... The plasmids were transfected with Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA) and siRNAs transfected with RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Positive clones were confirmed by plasmid sequencing (3130 Genetic Analyzer, Applied Biosystems). Resulting sequences were analyzed using Sequence Scanner v 2.0 (Applied Biosystems) ...
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Capacity to erase gene occlusion is a defining feature distinguishing naive from primed pluripotencybioRxiv - Molecular Biology 2021Quote: ... PCR products were cloned into plasmid using TOPO TA Cloning Kit (Invitrogen) and individual clones were picked for Sanger sequencing of the inserts.
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bioRxiv - Molecular Biology 2020Quote: ... plasmid DNA was mixed with calf thymus carrier DNA (Thermo Fisher Scientific) to give a total of 10 μg DNA ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were pre-complexed with 3 µg lipofectamine 2000 (Life Technologies 11668019) in 200 µl Opti-MEM (Life Technologies 51985091 ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... The entire construct was cloned into a pJet1.2 plasmid backbone (Thermo Fisher) using Gibson assembly master mix (NEB)79 ...
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bioRxiv - Synthetic Biology 2020Quote: ... cultures were prepared using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Part plasmids were verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Cancer Biology 2019Quote: ... The plasmid DNA/siRNA and lipofectamine were individually diluted in Optimem (Gibco) and incubated for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... and GeneJet gel or plasmid purification kits were purchased from Thermo Scientific. Endotoxin-free plasmid DNA isolation kits were purchased from Qiagen (cat ...
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bioRxiv - Molecular Biology 2019Quote: The Eco72IM sequence was PCR-amplified from the Eco72IRM plasmid (Thermo Fisher) and recombined with the pDONR 221 (Gateway ...
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bioRxiv - Developmental Biology 2020Quote: ... HEK293T cells were transfected with indicated plasmids using Lipofectamine 2000 (Life Technologies). After 48h ...
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bioRxiv - Molecular Biology 2019Quote: ... plated and extracted using GeneJET Plasmid Midiprep Kit (Thermo Fisher Scientific, USA). Number of colonies was estimated to be around 10000 individual clones.
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bioRxiv - Genetics 2019Quote: ... Plasmids were transfected into podocytes or HEK293 cells using Lipofectamine 2000 (Invitrogen). HEK293 cells stably expressing p3xFLAG-ADCK4 or BAP were selected and maintained with 1 mg/mL G418.
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bioRxiv - Molecular Biology 2019Quote: Three incisions-bearing plasmid (∼10 μg) was column purified (Invitrogen, cat#K220001) to check for digestion ...
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bioRxiv - Genomics 2020Quote: ... G8P plasmid (1 μg) were transfected into cells using Lipofectamine 3000 (Invitrogen). At 24 h post G8P transfection ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmid transfections and siRNA knock-downs were done using Lipofectamine 3000 (Invitrogen) in antibiotic-free media ...
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bioRxiv - Molecular Biology 2019Quote: ... and the template plasmid was digested with Turbo DNase (Thermo Fisher #AM2239). Transcribed mRNAs were purified LiCl precipitation and were used for injection ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids containing PSHCP were transformed into BL21 (DE3) star cells (Invitrogen, C601003). PSHCP mutants were generated using Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... HaCaT cells were transfected with plasmid minigene and selected using G418 (Gibco). Expression and splicing of the minigene was assessed by RT-qPCR using primers presented in the reagent table.
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bioRxiv - Immunology 2021Quote: ... all transfections of plasmids were performed using Lipofectamine 2000 reagent (Life technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... cells were transfected with expression plasmids using Lipofectamine 3000 (Thermo Fisher; L3000015) following the manufacturer’s instructions and 3 μL of lipofectamine per μg of plasmid DNA ...
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bioRxiv - Biochemistry 2021Quote: ... E14 cells were transfected with plasmids carrying sgRNAs with Lipofectamine (Life Technologies). After two days ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid purification was performed from E.Coli One Shot TOP10/P3 (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were transformed in chemically competent bacteria strains Top10 (Thermo Fisher, C303003) and Stbl3 (Thermo Fisher ...