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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... incubated 20 min at RT and added to 500 mL 3×106/mL HEK 293S cells culture in Freestyle 293 expression medium (ThermoFisher). The cells were cultured for 5 days ...
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bioRxiv - Neuroscience 2023Quote: To quantify iPSC proliferation 3 passages per cell line were treated with 10 µM EdU (Thermo Fisher Cat. no. C10338) 3-4 days after last passage for 1 and 4 hours ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: Intracellular expression of HLA-DR in IFN-γ-stimulated HaCat cells or intracellular expression of pS6k and pAMPK in LCL cells were determined as follows: 3*105 cells were first resuspended in PBS and stained for fixable viability dye (eFluor780; Invitrogen) for 20 min at 4°C protected from light ...
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bioRxiv - Cancer Biology 2024Quote: ... apoptosis was evaluated in NCI-H1975 or HeLa cells using CellEvent Caspase-3/7 Green ReadyProbe Reagent (Invitrogen, Cat#R37111) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3×2 minutes with DPBS before a permeabilization solution (0.1% Triton-X (Fisher Scientific, 9002-93-1) in DPBS ...
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bioRxiv - Systems Biology 2024Quote: ... fresh medium was added to dilute the tryplE and cells were centrifuged at 400 x g for 3 min then washed twice with Phosphate Buffered Saline (PBS) (Gibco) with centrifugation in-between ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed with 0.01 M PBS + 0.1% triton 3 times (10-minute incubations) and nuclei were stained with NucBlue Fixed Cell Stain ReadyProbes reagent (Invitrogen, R37606) diluted in 0.01 M PBS for 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... cell vials were thawed 2-3 min in a 37°C water bath and transferred in T25 cell culture flasks (Gibco) with 5ml of hASC culture medium containing Minimum Essential Mediun (αMEM ...
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bioRxiv - Developmental Biology 2019Quote: Prab-3::GFP was generated by cutting out Prab-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Developmental Biology 2019Quote: Pelt-3::GFP was generated by cutting out Pelt-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Cell Biology 2021Quote: ... 1–2 mg of VLDL were combined with 100 μL of 3 mg/mL 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD; Invitrogen) in DMSO and then re-isolated by ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... 50 nM siRNA (IMPDH2 assay ID: s7417, sense: 5’-CCAAGAAAAUCACUCUUtt-3’; anti-sense: 5’-UUAAGAGUGAUUUUCUUGGtc-3’, Ambion by Life technologies ...
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bioRxiv - Genetics 2022Quote: ... mcherry: 5’-ATGGTGAGCAAGGGCGAGGAG-3’ and 5’-CTTACTTGTACAGCTCGTCCATG-3’) from pKHR8-foxc1aKI and separately subcloned into pJET2.1 (ThermoFisher) to give pJET-mVenus and -mCherry ...
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bioRxiv - Biophysics 2022Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and (ii) 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) were both from Invitrogen. The standard lipids (i ...
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with fresh ECGM and 3 times with ECGM/Hoechst 33342 (NucBlue™ ThermoFisher). After 10min incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...
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bioRxiv - Neuroscience 2024Quote: ... The siRNA sequence for YTHDF2 is 5′-AAGGACGTTCCCAATAGCCAA-3′ and for HIF1α is 5’-GCTGATTTGTGAACCCAT-3’ (Ambion). After 48 h from the initial transfection ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was measured by quantitative real real-time polymerase chain reaction (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with primers and probe specific for the CCHFV L gene.
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bioRxiv - Evolutionary Biology 2021Quote: ... we harvested virus from each virus line under sterile conditions by collecting up to 10 virus killed cadavers per line and transferring these to sterile 15 mL disposable tissue grinders (ThermoFisher Scientific, U.S.A.). Infected larvae were recognizable by their opaque ...
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bioRxiv - Genomics 2019Quote: ... ZHBTc4 cells were transduced with pLV-pCAGGS-Tir1-V5 and PLEX-mCherry-OCT4-AID packaged lentiviral vectors (i.e Tir1-V5 and mCherry-Oct4-AID virus, respectively) and were selected with 2 μg/ml Puromycin (Thermo Fisher Scientific #A1113803) for 10 days ...
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bioRxiv - Microbiology 2020Quote: Proteins were harvested four to five days post virus infection in RIPA Lysis and Extraction Buffer (Boston Bio) with 1X Protease Inhibitor and 1X Phosphatase Inhibitor (Thermo Fisher Scientific). Protein Concentration was determined using BCA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Purity and titer of virus were then assessed by SDS-PAGE and SYPRO ruby staining (S-12000, Life technologies, Carlsbad, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using TaqMan Fast Virus 1-Step Master Mix (Thermofisher Scientific, US) with primers and probe specific for the SARS-CoV-2 E gene following guidelines by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assayv1991527e5122341d99287a1b17c111902.pdf ...
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bioRxiv - Microbiology 2021Quote: ... HCV RNA was quantified by one-step reverse transcription-quantitative PCR using 50 ng of total intracellular RNA and TaqMan® Fast Virus 1-Step Master Mix (Applied Biosystems) with primers and probe targeting the HCV 5’ nontranslated region as described previously [82] ...
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bioRxiv - Microbiology 2022Quote: The ZEBOV RT-PCR assay was performed in a 25 µL reaction mixture containing TaqMan Fast Virus 1-step master mix (Thermo Fisher Scientific), 5 µl template RNA ...
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bioRxiv - Microbiology 2019Quote: ... total RNA was reverse transcribed using virus-specific RT primers or oligo-dT primers and the SuperScript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). Real-time quantitative PCR was performed on the Applied Biosystems 7500 system with QuantiTect SYBR Green PCR Master Mix (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: cDNA sequences from the leader through the first half of the rabies virus P gene were amplified using Platinum SuperFi Green Master Mix (Invitrogen (Thermo Fisher), USA ...
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bioRxiv - Microbiology 2020Quote: ... apical poles were gently washed with warm PBS and then infected with a 150-μL dilution of HRSV-A (Long) virus in OptiMEM medium (Gibco, ThermoFisher Scientific) at a MOI of 1 ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... One microgram of RNA was reverse transcribed into cDNA using oligo-dT and MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase, Invitrogen, Thermo Fisher Scientific). Before cDNA synthesis ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: Viral RNA for real-time PCR assay was extracted from 140 ml of virus isolate (previously tittered as described above) and serum samples using Invitrogen PureLink RNA Mini Kit (Invitrogen, Valencia, CA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
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bioRxiv - Immunology 2020Quote: ... Virus heat inactivation at 56 °C for 30 minutes and total RNA including virus RNA extraction was performed using TRIzol™ Plus RNA Purification Kit (ThermoFisher, USA). cDNA synthesis was performed using GoScript™ Reverse Transcription System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... RNA extracted from animal samples or RNA standards were then measured using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Genetics 2022Quote: ... 30 μg of the virus) was digested with 1:100 (w/w) ratios of trypsin (Pierce Trypsin Protease mass spectrometry grade; Thermo Fisher Scientific)/virus for various time points at 25°C (31 ...
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bioRxiv - Evolutionary Biology 2022Quote: Virus RNA from nasal swabs was extracted from 50 µL of supernatant using the MagMAX-96 AI/ND Virus RNA Isolation Kit (Ambion, Austin, TX) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) of the extracted RNA samples (1 µg) was performed using AMV (Avian Myeloblastosis Virus) Reverse Transcription Kit (Invitrogen, Waltham, USA) and oligo-dT18 (1 µM ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The first-strand cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), using 100 ng of purified RNA ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus supernatant was collected after 24 hrs and 48 hrs and filtered with a 0.45-mm syringe filter (Thermo Fisher, CA, USA). pLenti-shCtrl (negative silencing control ...
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bioRxiv - Neuroscience 2023Quote: ... from the Stanford Gene Vector and Virus core unless otherwise specified) and Ctb retrograde tracers (1 ug/ul; Invitrogen #C34777, #C34776, #C34775) were infused with a syringe pump (Harvard Apparatus ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... One μg DNA or 4 μL cDNA was used to PCR-amplify virus barcode (VBC) using Platinum SuperFi II DNA Polymerase (12361010, Invitrogen, Waltham, MA). Information on primer sequence including frameshifting nucleotide ...
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...