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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Hela cell tryptic peptides were obtained from the commercially prepared Pierce HeLa Protein Digest Standard (Thermo Fisher). Each vial was reconstituted in 2% acetonitrile (ACN)/1% formic acid (FA ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 S protein-expressing plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen, USA). 24 h after transfection ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ACE-2 protein (hsACE-2, residues S19 to D615) was transiently transfected into HEK293E cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Immunology 2021Quote: ... Expression of gp120 proteins occurred as previously described in 293F cells by transient transfection with 293Fectin (Invitrogen) and purification by metal affinity and Size Exclusion Chromatography (Alam et al. ...
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bioRxiv - Immunology 2021Quote: ... or B.1.351 Spike protein (Table 2) were produced using HEK293T cells transfected with Lipofectamine 3000 (ThermoFisher) using IgE-SARS-CoV-2 S plasmid variants (Genscript ...
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bioRxiv - Microbiology 2022Quote: ... cells were first lysed using B-PER Complete Bacterial Protein Extraction Reagent (Thermo Fisher Scientific, Waltham, MA). To do this ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...
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bioRxiv - Microbiology 2023Quote: DB1 antibody was purified by binding clarified cell supernatant to Protein A Agarose beads (Thermo Fisher Scientific), with elution into 100 mM glycine pH 3.0 and was quickly neutralised with 1 M Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-50 μg of cell protein samples were resolved on Novex 4-20% Tris-Glycine Gels (Invitrogen) and transferred onto polyvinylidene fluoride membranes (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... whole cell lysates or immunoprecipitated samples were separated on NuPAGE 4-12% Bis-Tris protein gels (Invitrogen) in MOPS buffer and then transferred to PVDF membrane (Millipore Sigma) ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The cell lysate containing proteins were pre-treated with 0.1 mg/ml RNase A (Thermo Scientific, EN0531) for 1 h at 37°C to degrade all RNA–DNA hybrids ...
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bioRxiv - Microbiology 2023Quote: ... Passage 1 of viruses was used to express the proteins in High Five™ Cells (Thermo Fisher). The cells harvested 48 hours post inoculation were lysed in 50 mM Tris.HCl pH 8 ...
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bioRxiv - Immunology 2024Quote: ... the cell lysates were incubated with the appropriate IgG and Protein G magnetic beads (Thermo Scientific, 88847) for 2 hours at 4 °C for pre-clearing ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells transfected with GFP proteins were grown in a 96-well optical plate (Thermo Fisher Scientific) or 24-well lidded plates ...
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bioRxiv - Biochemistry 2024Quote: Whole-cell protein was harvested using the Pierce Co-Immunoprecipitation (Co-IP) Kit (ThermoFisher Scientific, Waltham, MA) and subcellular protein fractionation was performed on 1.0 x 107 cells using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Fc fusion proteins were expressed in ExpiCHO cells using the ExpiCHO expression system kit (Thermo Scientific, A29133) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were lysed in M-PER™ Mammalian Protein Extraction Reagent (Thermo-Fisher Scientific, CA, USA) supplemented with cOmplete protease inhibitor cocktail (Roche) ...
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bioRxiv - Bioengineering 2023Quote: ... HEK293 6E cells were used for transient production of these proteins following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection by centrifugation at 6,000 rpm for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were measured for protein concentration using Bradford analysis (Thermo Fisher #23209, Bio-Rad #500-0006). Totals of 10-30 µg of protein lysate and 10 µL of dual color protein standard ladder (Bio-Rad #161-0374 ...
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bioRxiv - Molecular Biology 2022Quote: ... AHA incorporated in proteins was detected using dedicated Click-iT Cell Reaction Buffer Kit (Thermo Fisher Scientific) and Alexa Fluor 594 Alkyne (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: 10 μg of total cell lysate protein was prepared in 1X (final) LDS Bolt™ buffer (Invitrogen) supplemented with 10% b-mercaptoethanol and heated for 5 minutes at 98ºC ...
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bioRxiv - Microbiology 2023Quote: ... Biofilm cells were resuspended in 200 µl of B-PER II Bacterial Protein Extraction Reagent (Thermo Scientific) and incubated for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Protein extraction was performed by pelleting cells and resuspending in cold RIPA buffer (Thermo Fisher Scientific, #89901) supplemented with phosphatase/protease inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... HEK 293T cells were transiently transfected with cDNA constructs encoding proteins of interest using Lipofectamine 2000 (Invitrogen) 16-24 hours before the start of the experiment ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were biotinylated with 0.5mg/ml EZ-link-Sulfo-NHS-SS-Biotin (Thermo Fisher, A39258) in a PBS buffer with 2.5mM CaCl2 and 1mM MgCl2 on ice for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Large-scale protein expression was performed by infection of High Five cells (Trichoplusia ni, Thermo Fisher, B85502) in Insect-Xpress medium (Lonza ...
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bioRxiv - Cancer Biology 2023Quote: Protein extraction was performed by pelleting cells and resuspending in cold RIPA buffer (Thermo Fisher Scientific, #89901) supplemented with phosphatase/protease inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: Nuclear proteins were extracted from THP1 cells with NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher, 78833). Biotin-labeled double-stranded oligonucleotide probes corresponding to the DNA sequences containing and surrounding the rs17514846 site (5’-AGTTGCGCCTGA[C/A]GCCTGCTTTCTT-3’) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the biotinylated cell surface proteins were affinity-purified on streptavidin magnetic beads (Thermo Scientific, cat#88816). After stripping off the nonspecifically bound proteins by several rounds of washing with the lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Subcellular fractionation was performed using the Subcellular Protein Fractionation Kit for Cultured Cells (Cat# 78840, Thermo Scientific) following the manufacturer’s protocols ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Recombinant PLD3 and PLD4 proteins were expressed in Expi293F cell line according to the manufacturer’s instructions (ThermoFisher). In brief ...
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bioRxiv - Cancer Biology 2024Quote: The BG4 protein was purified from the cell lysate using HisPur cobalt resin (2.5 mL, Thermo Scientific). The resin was washed with Milli-Q water (12.5 mL ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to quantify protein from the cell lysate by using a multi-plate reader (Thermo Scientific). Equal amount of proteins from each sample was resolved by SDS-PAGE and further probed with specific antibodies against β-catenin (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: Total cellular protein was extracted from frozen cell pellets using the Pierce™ RIPA buffer (ThermoFisher 88901) with protease inhibitor cocktail added (Sigma P8340) ...
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bioRxiv - Biochemistry 2024Quote: ... The obtained supernatants (cell lysates) were incubated at 4°C with Dynabeads Protein G (Thermo Fisher Scientific) pre-bound with antibodies ...
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bioRxiv - Genomics 2024Quote: ... Total protein from the cultured cells was isolated by RIPA Lysis and Extraction Buffer (ThermoFisher Scientific, 89900) with Halt™ Protease Inhibitor Cocktail (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2020Quote: GFP-Vimentin expressing HeLa cells were cultured on sterile glass slide (10 x 10 mm) and soft collagen hydrogel (3 mg/ml, Gibco) in 24 well plate as described previously84 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
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bioRxiv - Cell Biology 2020Quote: ... A Lsm12-KO cell line of HEK293 cells was generated with Synthego’s chemically modified sgRNA 5’-CCAGAAUGUCCCUCUUCCAG-3’ and GeneArt Platinium Cas9 nuclease (ThermoFisher Scientific) that were transfected together into cells using Lipofectamine CRISPRMAX Cas9 transfection reagent (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... A Neuropixels probe (option 3 phase A) with 374 recording sites was briefly (∼2 minutes) dipped into the diI cell-labeling solution (ThermoFisher) to visualize probe tracks ...
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bioRxiv - Bioengineering 2022Quote: ... SKOV-3 cells were cultured in RPMI 1640 medium supplemented with 10% FBS and penicillin-streptomycin all purchased from Gibco/ThermoFisher Scientific.
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed 3 times with MEM containing 10% fetal bovine serum and 1X penicillin/streptomycin (Thermo Fisher Scientific) to terminate the enzymatic digestion ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ul of each sample and 3% input of each cell lysate was loaded on a 4-12% Bis-Tris pre-cast SDS-page gel (Invitrogen) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were centrifuged at 700xg for 3 minutes before subculture into fresh medium containing 1X RevitaCell Supplement (Life Technologies, #A2644501).
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bioRxiv - Immunology 2019Quote: Mucosal curettage samples and epithelial cell cultures (incubated with or without S. pneumoniae for 3 hours) were collected in RNALater (ThermoFisher) and stored at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed 3 times with PBS (5 min, RT) and mounted using ProLong® Gold antifade mountant (ThermoFisher, P36930). STED microscopy was performed using a 100× objective on the STEDYCON 2-colour STED imaging system (Abberior Instruments) ...
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bioRxiv - Cell Biology 2019Quote: ... hearts from Col1a1-3.6GFP mice were digested and Col GFP-cells were grown for 3 days in high (4.5g/L) glucose DMEM (Thermo Fisher), supplemented with 10% v/v fetal bovine serum (FBS) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Wells were quickly washed once with 0.25% trypsin (by pipetting up and down 3 times) and twice with PBS to remove 3T3 cells before collecting keratinocytes into 1 ml Trizol (Invitrogen). Details of the samples used for polyI:C challenge across the 24 wolf keratinocyte cultures are provided in Supplementary Table S1.
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies ...