Labshake search
Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Human lung adenocarcinoma epithelial Calu-3 cells (ATCC) were maintained in a high-glucose DMEM containing sodium pyruvate and GlutaMAX (Gibco) with 10% FBS and 1% penicillin/streptomycin at 37°C with 5% CO2 ...
-
bioRxiv - Bioengineering 2021Quote: ... decellularized apple scaffolds (prior to MC3T3-E1 cell seeding) (n=3) were thoroughly washed with phosphate buffered saline (PBS; ThermoFisher) and incubated in 1 mL of 10% (v/v ...
-
bioRxiv - Bioengineering 2020Quote: ... hPSCs were washed 3 times with PBS and dissociated into single cells by incubation in 1 mL of TrypLE Express (GIBCO) at 37°C for 3 min ...
-
bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from HTM cells ± DEX at 7 d (N = 3 per group and donor) using PureLink RNA Mini Kit (Invitrogen). RNA concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were washed 3 times for 10 minutes and then mounted with DAPi containing mounting media (Invitrogen, Carlsbad, CA, USA).
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with PBS prior to loading with 3 μM of the Ca2+ indicator Fura-2 AM (Invitrogen) diluted in a modified Krebs-Ringer buffer [KRBH ...
-
bioRxiv - Neuroscience 2020Quote: ... a selected cell terminal was puffed for 5 s with a solution containing 3-5 μM FM1-43 (Molecular Probes) and (in mM) ...
-
bioRxiv - Microbiology 2021Quote: ... Calu-3 bronchial adenocarcinoma cells (ATCC ® HTB-55™) (11) were cultured in DMEM-F12 (1X) (Thermo-Fisher Scientific) supplemented with 10% FBS and 1% penicillin-streptomycin ...
-
bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... 50μg of the plasmid was then transfected into Expi293F cells at a density of 3×106 cells/mL in a 250mL shaking flask using the ExpiFectamine 293 Transfection Kit (Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... to label the glycocalyx (GX) and for 3 min with CellMask Green plasma membrane live cell imaging dye (Life Technologies) prepared in endothelial growth medium to label ECs ...
-
bioRxiv - Molecular Biology 2020Quote: ... reinhardtii cells were grown at room temperature for 3-4 days in the light in 20 mL of TAP media (ThermoFisher). The algae were collected by centrifugation at 10,000 x g for 1 min ...
-
bioRxiv - Developmental Biology 2021Quote: Donor embryos were injected at the 1-cell stage with 3-5nL of 1% Dextran-FITC 10,000 MW (Molecular Probes) and 0.05% Phenol Red (to see the solution ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cell death was assessed by measuring caspase 3 cleavage using a CellEvent Caspase3/7 Green Flow Cytometry kit (Thermo Fisher), according to the manufacturer’s descriptions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
-
bioRxiv - Cell Biology 2020Quote: ... with the exception of Calu-3 cells that were grown in Eagle’s minimal essential medium (MEM+GlutaMAX; Gibco™, ThermoFischer) supplemented with 10% FCS 0.1mM non-essential amino acids (NEAA) ...
-
bioRxiv - Microbiology 2021Quote: ... at a density of 3×103 cells/well in phenol-red free Dulbecco’s modified Eagle medium (DMEM; Gibco #31053-028) supplemented with 2 mM L-glutamine (Gibco #25030024) ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were loaded with 3 μM Fura-2-AM and 0.01% (v/v) Pluronic F-129 (both from Molecular Probes) in DMEM medium at 37 °C for 20 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were then passaged 1:3 as clumps using 15 min 37°C incubation with 1mg/ml collagenase IV (Invitrogen) onto plates coated with 1:50 GFR-Matrigel (Corning ...
-
bioRxiv - Microbiology 2022Quote: ... macrophages were seeded with a density of 3 x 105 cells/well on coverslips and stained overnight with 60 μg/ml tetramethylrhodamine dextran (3000 MW, Invitrogen). At the next day ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1µM PRMT5 inhibitor or negative control treated (3 or 6 day) OCI-AML-20 cells were incubated with 10 µM EdU (Invitrogen) for 2 h at 37 °C ...
-
bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
-
bioRxiv - Cell Biology 2022Quote: ... organoid fragments collected from 3 wells of a 24 well plate were incubated with TrypLE express cell dissociation enzyme (ThermoFisher) supplemented with Y-27632 (10 µM ...
-
bioRxiv - Microbiology 2022Quote: A total of 1.5 µg/well of VA1 IVT RNA was transfected into BHK-21 cells in 12-well plates using 3 µL/well of Lipofectamine MessengerMax (Invitrogen) and following the manufacturer’s protocol ...
-
bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Epithelial cell culture medium containing Y-27632 consisted of DMEM/F12 in a 3:1 ratio containing 1x penicillin–streptomycin (Gibco), 5% fetal bovine serum (Gibco ...
-
bioRxiv - Microbiology 2020Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
-
bioRxiv - Cell Biology 2021Quote: INS-1 832/3 cells (a gift from Professor Christopher Newgard, Duke University, USA) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
-
bioRxiv - Microbiology 2021Quote: ... total RNA extracted from reovirus-infected cells was denatured at 95°C for 3 mins and subjected to reverse transcription using SuperScriptTM III Reverse Transcriptase (Invitrogen). A primer (S4 forward ...
-
bioRxiv - Neuroscience 2020Quote: ... 3 x 104 cells were replated in 30 μl microdrops of N2B27 medium on 13 mm glass coverslips (Fisher Scientific) pre-treated with poly-L-lysine (PLL ...
-
bioRxiv - Microbiology 2021Quote: ... Human lung adenocarcinoma epithelial Calu-3 cells (ATCC, Manassas, VA, USA) were maintained in a high-glucose DMEM containing sodium pyruvate and GlutaMAX (Gibco) with 10% FBS and 1% penicillin/streptomycin at 37°C with 5% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... 4 million splenocytes cells were stimulated ex vivo for 3 hours at 5% CO2 at 37°C in Minimum Essential Media (Gibco) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were then triturated by gentle pipetting and washed 3 times with DRG growth medium (Neurobasal medium (Gibco 21103049) containing 2% B27 (Invitrogen 17504044) ...
-
bioRxiv - Immunology 2022Quote: ... Expi293F cells grown to a density of 3 × 106/ml were transiently transfected with 640 µl of Expifectamine (Thermo Fisher) and 200 µg of the corresponding expression plasmid ...
-
bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...
-
bioRxiv - Bioengineering 2022Quote: ... The medium was replaced every 2-3 days and cells were split once they reached 75-80% confluence using TrypLE Express (Gibco). LNCaP and U87 cells were gifted by Professor Lisa Butler and Professor Stuart Pitson ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were transfected according to manufacturer’s instructions using 1 µg of total DNA complexed with 3 µg Lipofectamine2000 (ThermoFisher 11668019) in 200 µL Opti-MEM (ThermoFisher 51985091) ...
-
bioRxiv - Paleontology 2019Quote: ... and 3µl (chimpanzee 3 and 4) of each cloning reaction product were used for transformation of DH5α Competent Cells (Invitrogen, 18265017). Colonies were grown overnight on LB plates containing 100μg/ml ampicillin ...
-
bioRxiv - Immunology 2020Quote: ... centrifuged at 300g for 5 min and resuspended in 3 mL of 1X ebioscience Red Blood Cell Lysis Buffer (ThermoFisher) for 5 min at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... TIB-47) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
-
bioRxiv - Cell Biology 2019Quote: ... SKOV-3 and HeLa cells were purchased from the American Type Culture Collection (ATCC) and cultured in DMEM (Thermo Fisher) supplemented with 10% FBS as previously described [40] ...
-
bioRxiv - Microbiology 2020Quote: ... Footpad lesions was excised and then homogenized with a cell strainer in 3 ml of Schneider’s Drosophila medium (Gibco, US) supplemented with 20% heat-inactivated fetal calf serum and Penicillin-Streptomycin (0.1%).
-
bioRxiv - Cell Biology 2021Quote: ... and 143B cybrid cells were passaged every 3-4 days at 80% confluence and trypsinised using 0.25% trypsin-EDTA (Gibco #25200056). Similarly ...
-
bioRxiv - Neuroscience 2021Quote: ... Then cell suspensions were triturated by gentle pipetting and washed 3 times with the DRG growth medium (Neurobasal medium (Gibco) containing 2% B27 (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed 3 times and stained with a goat anti-hamster IgG (H+L) Alexa Fluor 488 (ThermoFisher Scientific). Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII analytic cytometer (BD Bioscience).
-
bioRxiv - Neuroscience 2021Quote: ... Cells were washed 3 times in PBT and mounted on slides with DAPI (Prolong Gold Antifade with DAPI (Life Technologies). Imaging was performed on an inverted Zeiss LSM700 Laser Scanning Confocal and processed on Fiji (ImageJ).
-
bioRxiv - Cancer Biology 2020Quote: ... HGrC1 cells (15 samples including 3 replicates for 5 conditions) were lysed using TRIzol reagent (catalog #15596026, Thermo Fisher Scientific) and RNA was extracted with Direct-zol RNA MiniPrep kit (#R2052 ...
-
bioRxiv - Immunology 2021Quote: ... 4 million isolated splenocytes or inguinal lymph node cells were stimulated ex vivo for 3 hours at 5% CO2 at 37°C in Minimum Essential Media (Gibco) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2021Quote: ... Red blood cells were then lysed by adding 3 ml of ammonium-chloride-potassium (ACK) lysis buffer (Invitrogen, Carlsbad, CA) for 4 min at room temperature ...