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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined using BCA protein assay kit (Thermo Fisher). 30-40 µg of protein with SDS loading dye (4x ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were quantified using Pierce BCA Protein Assay Kit (ThermoFisher) on an Infinite M200 plate reader (Tecan) ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was quantified using a BCA protein assay (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Proteins were quantified with the Qubit Protein Assay Kit (Invitrogen, Q33212), dilute to 1 µg/µl followed by denaturation using 5 X SDS loading buffer (CWBIO ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein amount was analyzed by BCA protein assay (ThermoFisher Scientific). Protein was denatured by adding NuPAGE LDS buffer and DTT reducing buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris Protein gels (Invitrogen) and then horizontally transferred to the Immobilon-FL PVDF membrane (#IPFL00010 ...
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bioRxiv - Cancer Biology 2023Quote: ... After protein quantification (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific), 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels ...
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bioRxiv - Cell Biology 2023Quote: ... Protein complex sizes were determined with NativeMark Unstained Protein Standard (ThermoFisher). After resolving the protein complexes under native conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μl Dynabeads Protein A and Protein G (Thermo Fisher Scientific) were pre-incubated overnight with rotation in the presence of 5 mg/ml BSA and 10 μg antibody (MYCN (B8.4.B) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were quantified using BCA Protein Assay Kit (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined with the Qubit protein assay (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Protein concentration was measured using BCA Protein Assay (Fisher Scientific, PI23227). 20 µg of protein was removed for total protein measurements ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was assessed using BCA protein assay (Thermo Fisher Scientific). Protein electrophoresis was performed using 4–15% Mini-PROTEAN TGX Precast acrylamide gel (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was measured by BCA protein assay (Thermo Fisher Scientific). Fifteen to thirty micrograms of lysates were separated on 4-15% precast Mini-PROTEAN® TGX™ gels (Bio Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Protein estimation was carried out using BCA protein estimation kit (Invitrogen).
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration then determined using a Qubit protein assay kit (Invitrogen). The MUG fluorescent assay was performed in duplicates with 20 µl protein extract in MUG assay buffer (50mM phosphate buffer (pH 7.0) ...
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bioRxiv - Cancer Biology 2021Quote: Total proteins were isolated from GC cell and the concentration was measured with BCA Kit (ThermoFisher, USA). Proteins were separated by 10% SDS-PAGE gel and transferred to 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Biophysics 2021Quote: ... Cell walls were removed by Zymolyase [38] and total cellular proteins were extracted with RIPA reagent (Invitrogen) that contains Protease Inhibitor Cocktail (Bimake) ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates containing all proteins were loaded on an SDS-PAGE precast Tris-Bis NuPAGE gel (Invitrogen), using MOPS solution (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with siRNA duplexes targeting the corresponding protein or scrambled mixes using Lipofectamine RNAiMax (Invitrogen) reagents as per manufactures recommendations ...
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bioRxiv - Immunology 2021Quote: ... Total cell protein was extracted by adding 1 mL RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific), 10 μL Halt™ Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... plasma and cell lysates was quantified using a micro BCA™ protein assay kit (23235, ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration of cell lysates was determined with the BCA assay kit (Thermo Fisher Scientific, Schwerte, Germany).
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bioRxiv - Cell Biology 2019Quote: ... equal amounts of cell lysates were incubated with the indicated antibodies conjugated to protein G beads (Invitrogen) or anti-HA beads (15µl per IP ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of cell lysates were incubated with the indicated antibodies conjugated to protein G beads (Invitrogen) respectively from 4h to overnight at 4° C ...
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bioRxiv - Immunology 2021Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S ...
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bioRxiv - Genomics 2020Quote: ... Human PDCD1 or PDL1 cell surface protein staining was analyzed using anti-PD-1 (ThermoFisher clone MIH4) or anti-PD-L1 (ThermoFisher clone MIH1 ...
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bioRxiv - Cancer Biology 2020Quote: Cells were harvested and protein was extracted using mPER lysis buffer (Thermo Fisher Scientific, Waltham, MA, USA) with protease and phosphatase inhibitors (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were transiently transfected with the corresponding fusion protein cDNA using Lipofectamine 3000 (Invitrogen, Paisley, Scotland, UK).
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration in the cell lysate and media was measured by BCA kit (ThermoFisher, MA, USA)as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Biotinylated cell-surface proteins were pulled down with streptavidin-coupled magnetic beads (#11205D; Thermo Fisher Scientific, US). The beads were isolated ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed with RIPA buffer and biotinylated proteins were precipitated with streptavidin-agarose beads (Thermo Fisher). Elution was carried out with Laemmli buffer (2% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... EGFR-KD and RON-K1114M proteins were produced using the Expi293 cell Expression System (Thermo Fisher Scientific) according to the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed 48 hours post-transfection with 1% SDS and quantified via Pierce Protein Assay (Thermofisher) and a spectrophotometer (Biotek ...
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bioRxiv - Cell Biology 2020Quote: The protein concentrations of total cell lysates were determined utilizing Pierce BCA Assay (Cat. 23225, Thermo Fisher). The protein concentrations of the EVs were determined utilizing Pierce Micro BCA Assay (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were blocked with 10% Pierce™ Protein-Free (PBS) Blocking Buffer (#37572; Thermo Fisher Scientific) in PBS for 1 h at room temperature ...
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bioRxiv - Genetics 2021Quote: Synaptic protein was extracted by manually dissociating cultured cells in 1x Syn-PER Reagent (Thermo Fisher Scientific) with added MS-SAFE Protease and Phosphatase Inhibitor (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... Whole cell lysate was extracted in M-PER™ Mammalian Protein Extraction Reagent (ThermoFisher Scientific, Cat# 78501) with gentle sonication and core histone proteins were extracted with the EpiQuik Total Histone Extraction Kit (Epigentek ...
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bioRxiv - Biochemistry 2020Quote: ... The cell pellet was resuspended and lysed in B-PER Bacterial Protein Extraction Reagent (Thermo Scientific #78248) supplemented with lysozyme (EMD #5950) ...
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bioRxiv - Cell Biology 2022Quote: ... we covalently labeled cell-surface proteins using a membrane-impermeable biotinylation reagent (NHS-SS-biotin; Thermo Fisher) as described previously (Yamada et al. ...
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bioRxiv - Microbiology 2019Quote: ... Gag was immunoprecipitated by incubating the cell lysates with HIV-Ig bound to DynabeadsTM Protein G (Invitrogen) for 1 hour on a rotating shaker at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells were transfected with the pCS2-FLAG empty and protein coding constructs using Turbofect (ThermoFisher Scientific). After 48 hrs the cells were crosslinked with 1% formaldehyde (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants containing fly and cell lysates were quantified with Pierce™ BCA Protein Assay Kit (Thermo Scientific) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Transfection of the immune cells for transiently expressed proteins was accomplished with lipofectamin 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell fractions were prepared using the Subcellular Protein Fractionation Kit according to manufacturer instructions (Thermo Fisher Scientific). Immunoblots were probed with different antibodies ...
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bioRxiv - Microbiology 2020Quote: Uninfected cells were lysed in 1 mL M-PER Mammalian Protein Extraction Reagent (Thermo Fisher, Waltham, Massachusetts) directly in culture wells and boiled for 10 minutes ...
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bioRxiv - Genetics 2021Quote: Plasmids expressing GST- and His-tagged proteins were transformed into BL21(DE3) competent cells (Thermo Scientific, EC0114) and grown in LB media at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The cell pellet was resuspended in 200 μL M-PER mammalian protein extraction reagent (ThermoFisher Scientific, #78501) at 50,000 cells per μL lysis solution plus 1x HALT protease inhibitor mix (ThermoFisher Scientific ...