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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Expi293F™ cells were seeded to a final density of 2.5-3 × 106 viable cells/ml and grown overnight at 37 °C in Expi293™ Expression Medium (Gibco). The following day ...
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bioRxiv - Molecular Biology 2021Quote: ... For cell collection and/amplification ESCs were trypsinized for 2 to 3 minutes with trypsin-EDTA (Invitrogen, Cat#25200-072) and the digestion was stopped by the addition of pre-warmed 15% FCS ...
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bioRxiv - Immunology 2021Quote: ... NKp46+ and stained as indicated by manufacturer’s protocol with Cell Event Caspase 3/7 Green Flow Cytometry Assay Kit (Molecular Probes). All samples were analyzed using BD Canto FACS instrument ((BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed 3 times with PBS and stained with MitoTracker® red (200 nM, Thermo Fisher Scientific, Waltham, MA) for 30 minutes at 37 °C at 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours before transfection 1.5 × 105 cells were seeded in a 6-wells plate with 3 mL of antibiotic-free medium (DMEM (Gibco™) high glucose ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times with PBS* and mounted using Prolong Gold that included DAPI for nuclear staining (Thermo Fisher). Images were taken with a Zeiss LSM780 confocal microscope equipped with a 63x oil (NA = 1.4 ...
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bioRxiv - Genetics 2022Quote: ... The cells were passaged every 3-4 days after they achieved confluency of 80-90% using Trypsin 0.25% EDTA (Life Technologies).
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... the cell cultures were washed 3 times with 37 °C DPBS+ and fixed using 4% w/v paraformaldehyde (PFA; Affymetrix) in PBS for 2.5 h ...
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bioRxiv - Physiology 2023Quote: ... MEFs were seeded at density of 3 × 105 cells/ml in a T182 flask and cultured in DMEM containing 10% EV-depleted FBS (Gibco) for 48h prior to collection of conditioned media ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were co-cultured with HLA-B/CMV-restricted CD8+ T-cells for five hours (E:T ratio: 3:1) in RPMI 1640 medium (Life Technologies)(supplemented with 10 % (v/v ...
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bioRxiv - Molecular Biology 2022Quote: 20~60μg lysates from culture cells or mouse tissues were separated on 10% Bis-Tris or 3~8% Tris-Acetate Novex NuPAGE gels (Invitrogen) and transferred to nitrocellulose membrane following standard procedures ...
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bioRxiv - Cell Biology 2022Quote: ... mutant of ERK3 were reintroduced into shERK3 (3’UTR) background by lentiviral transduction and cells were double-selected with zeocin (100 µg/ml) (Cat# R25001, Invitrogen) and puromycin.
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bioRxiv - Cell Biology 2022Quote: ... each cell well was transfected with 3 μg of plasmid DNA complexed with TurboFect transfection reagent (ThermoFisher, Grand Island, NY) for HEK293T cells ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 3 times with PBS and mounted onto glass slides with Prolong gold antifade mountant (Invitrogen; catalog #P36930). Imaging was performed using a Zeiss LSM880 confocal microscope.
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bioRxiv - Microbiology 2024Quote: ... Tenfold dilutions were used to infect confluent Vero E6 cells in a 96-well plate in MEM (5% FBS, 1% penicillin-streptomycin, 1% kanamycin, 3% amphotericin B (Gibco)) at 37°C and 5% CO2 ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were seeded at the coated chamber slides with a density 200000 cells per well and the next day four hours before imaging cells were stained with 1 µg/ml Hoechst 33342 for 30 min at 37°C and washed 3 times for 10 min each with Live Cell Imaging Solution (Invitrogen). When applicable ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed and fixed for 15 min at room temperature (RT) using 3% methanol-free formaldehyde solution (Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured on a feeder layer of mitomycin treated J2 Clone of Swiss 3T3 Cells (J2-3T3s) in FAD media (3 parts Dulbecco’s Modified Eagle medium (Thermofisher, USA), 1 part Ham’s F12 media mixture (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA-treated cells were washed twice with 37°C M1 and replaced with M1 containing 1 μM TO-PRO-3 iodide (ThermoFisher) or washed once with 37°C M2 containing 5 mM ethylene glycol-bis(2-aminoethylether)-N ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 µL of 1 mM TO-PRO-3 Iodide in DMSO (Thermo) was added to stain dead cells and 1uL of DRAQ5 (Invitrogen) was added to stain viable cells ...
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bioRxiv - Developmental Biology 2024Quote: Monolayers and EBs from day 3 to day 8 were dissociated to single cells with TypLE 1X (Thermo Fisher, 12563029) for 5 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed 3 times with 1X PBS and treated with 1 μM C11-Bodipy 581/591 dye (Invitrogen) suspended in 1X PBS for 30 min in the dark at 37oC ...
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bioRxiv - Cell Biology 2024Quote: TRIM28 and TRIM24 knockdowns were performed similarly with the following modifications: 3×106 TRExRTA BCBL-1 cells in 100 μL of resuspension buffer T (Invitrogen) were nucleofected once with 2 uL of 100 μM of either Dharmacon human TRIM28 Accell SmartPool siRNA or OnTarget Plus human TRIM24 SmartPool ...
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bioRxiv - Cell Biology 2024Quote: ... On the day of electroporation organoids were dissociated into small clumps of 3-5 cells using mechanical pipetting and TrypLE incubation and resuspended in Opti-MEM Reduced Serum Medium (Gibco). 5 μg of the cloned REG1A sgRNA-plasmid ...
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bioRxiv - Neuroscience 2023Quote: To quantify iPSC proliferation 3 passages per cell line were treated with 10 µM EdU (Thermo Fisher Cat. no. C10338) 3-4 days after last passage for 1 and 4 hours ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3 times with PBS and incubated with secondary goat anti-rabbit Alexa Fluor 488 antibody (#A11008, Invitrogen) at 1:500 dilution in 1% BSA for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... and OVCAR-3 cells were maintained in RPMI-1640 media (HiMedia; AL162A) along with 20% fetal bovine serum (Gibco; 10270) in a 5% carbon dioxide ...
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bioRxiv - Cancer Biology 2023Quote: The SKOV-3 cells (5000 in number) suspended in Mcoy’s 5A media (HiMedia; AL057A) along with 10 % fetal bovine serum (Gibco; 10270) were added to the decellularized senescent and non-senescent matrices and allowed to adhere on the matrix for 25 mins ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell bodies were removed by centrifugation (1,150 g, 3 min, 4°C; Sorvall Legend XTR, Thermo Fisher Scientific). A sucrose cushion (10 ML of 25% sucrose in HMS ...
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bioRxiv - Molecular Biology 2023Quote: siRNA purchased from Eurofins MWG Operon or Sigma-Aldrich (Supplementary Table 3) were transiently transfected into cells using Lipofectamine RNAiMax (Invitrogen) at a final concentration of 25-60 nM in the culture medium following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... mouse embryonic fibroblasts 3T3-J2 cells (kind donation from Howard Green, Harvard Medical School, Boston52) with keratinocyte culture medium cFAD (3:1 DMEM (Gibco) to Ham F-12 Nut Mix (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... the COS-7 cells were transfected with plasmids encoding tubulin (3×mEmerald-ensconsin) and ER (mCherry-KDEL) by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol17 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then labeled with the Zn2+ indicator FluoZin-3 (2.5 μM) in HBSS following manufacturer instructions (Thermo Fisher Scientific). The cells were then washed once with HBSS and then placed back into HBSS before images were quickly acquired on an inverted Olympus IX70 fluorescence microscope with a 10X phase contrast objective (Olympus Neoplan 10/0.25 Ph ...
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bioRxiv - Cancer Biology 2023Quote: 293FT cells were transiently transfected with 3 μg of each of the HA-Clover KEAP1 constructs diluted into Opti-MEM (Gibco) and supplemented with lipofectamine 2000 transfection reagent (Invitrogen) ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Immunology 2023Quote: ... 2.5x106 TCR-deficient Jurkat or SKW-3 cells were electroporated with plasmid DNA using the Neon Transfection system (Thermo Fisher). Single-cell clones were expanded under selection (8 or 10 µg mL-1 blasticidin ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Neuroscience 2023Quote: ... The isolated cells were expanded in tissue culture flasks for 2-3 days in proliferation medium containing DMEM/F-12 media (Gibco), penicillin-streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... pcDNA-uAb (500 ng) plasmids were transfected into cells as triplicates (3×105/well in a 24-well plate) with Lipofectamine 2000 (Invitrogen) in Opti-MEM (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... cells in each well were firstly washed with flow cytometry staining buffer: 3% FCS with 2 mM EDTA (15575020; Invitrogen) in PBS− ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... The cells were resuspended at a density of 3 million cell per mL in RPMI1640 medium supplemented with 10% low endotoxin FBS (Gibco), 50LJμM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...