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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... from the Stanford Gene Vector and Virus core unless otherwise specified) and Ctb retrograde tracers (1 ug/ul; Invitrogen #C34777, #C34776, #C34775) were infused with a syringe pump (Harvard Apparatus ...
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bioRxiv - Immunology 2022Quote: ... and subjected to the one-step quantitative real-time reverse transcription-PCR assay (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat# 4444432) as described previously58 ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA synthesis was performed on 1 µg of total RNA with the RevertAid H Minus Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific) using random primers p(dN)6 (Roche) ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... One μg DNA or 4 μL cDNA was used to PCR-amplify virus barcode (VBC) using Platinum SuperFi II DNA Polymerase (12361010, Invitrogen, Waltham, MA). Information on primer sequence including frameshifting nucleotide ...
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bioRxiv - Plant Biology 2024Quote: ... 1.0 μg DNase-treated RNA was reverse transcribed in a reaction volume of 20 μl containing oligo-(dT)19 primer and Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). RT-qPCR analysis was then performed with 1.0 μl of 5-fold diluted cDNA using a SYBR premixed Ex Taq kit (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... levels quantitative real-time PCR (RT-qPCR) was performed as previously described22 using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, #4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein quantification of the glass bead protein extracts was conducted using the BCA Protein Assay kit (Thermo Fisher), and samples with equal amount of proteins were applied on SDS-PAGE gels ...
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bioRxiv - Neuroscience 2020Quote: ... and pre-cleaned with protein A/G beads (Dynabeads™ Protein A, 10002D, Dynabeads™ Protein G, 10004D, Invitrogen). Immunoprecipitation was conducted with a mixture of Aβ antibodies (6E10 ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Microbiology 2021Quote: Purified ThsA protein was freshly prepared and protein concentration was quantified using Qubit Protein Assay Kit (ThermoFisher cat # Q33212). Protein was diluted in wash buffer to a concentration of 147 ng/μl ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Neuroscience 2019Quote: Membrane proteins and cytosolic proteins were isolated using Mem-PER™ Plus membrane protein extraction kit (Thermo fisher scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... and total protein concentration was quantified using a BCA protein assay (Pierce BCA Protein Assay kit, 23225, ThermoFisher Scientific). Samples were diluted to 0.8 ug/uL with a 1X cell lysis buffer provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were measured using the Bio-Rad Protein Assay Kit or BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts (25 μg or 10 μg ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was quantified using the “Protein Quantification BCA Assay” kit (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the protein extract was then measured with a BCA Protein Assay Kit (Thermo Fisher Scientific). A volume of each protein extract corresponding to 14 mg protein was transferred into a new 5-mL tube containing 150 µL streptavidin beads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 500 ng ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 2 μg/μL with the IP lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations were determined using the BCA protein assay (Micro BCATM protein assay kit; Thermo Fisher Scientific, MA, USA) and CLARIOstar microplate reader (BMG labtech Durham ...
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bioRxiv - Neuroscience 2023Quote: The brains of P9-11 C57 mice were immersed and fixed for 3 days in cold 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide HCl (EDC, Thermo Fisher Scientific, in 0.1 M PB, pH 7.4) at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: Samples of salmonid CF and non-salmonid OF (2 µg of proteins for each sample) were subjected to a short migration on a NuPAGE system (Invitrogen, 3 minutes at 200 V-400 mA - 23 W) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Proteins were isolated using RIPA buffer and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Total RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were measured using a protein assay kit (Pierce™ BCA Protein Assay Kit, #23225, ThermoFisher Scientific, Cheshire, UK). Protein samples were resolved through 10% SDS-polyacrylamide gel and transferred to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing proteins was collected and the protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Scientific). Immunoblotting analysis was carried out as previously described.(8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... following which protein-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader.
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was measured through the Bicinchoninic Acid protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™ 23225). Equal amounts of proteins extracts (20-30 μg for mitochondrial samples or 40 μg for total kidney homogenates ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein in brain tissue was determined using a protein assay kit (Pierce™ BCA Protein Assay Kit; ThermoFisher, 23225) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Protein extraction was performed as described previously37 and protein concentration was determined using Pierce BCA protein assay kit (Thermo scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant containing the protein extract was collected and the total protein concentration was determined (BCA Protein Assay Kit, Thermofisher). Samples were reduced with DTT (15 mM ...
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bioRxiv - Biochemistry 2023Quote: ... Dried proteins were reconstituted in 0.2 % SLS and the amount of proteins was determined by bicinchoninic acid protein assay (Thermo Scientific). For tryptic digestion 50 μg protein was incubated in 0.5% SLS and 1 μg of trypsin (Serva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Protein purity and concentration were determined using Coomassie Imperial Protein Stain and Pierce bicinchoninic acid protein assay (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Total protein quantification was determined using Pierce™ BCA Protein Assay Kit (Thermo Scientific Pierce™ BCA Protein Assay Kit) and samples were stored at -80°C until use.
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bioRxiv - Pathology 2023Quote: ... aortic protein lysates were prepared in RIPA lysis buffer and protein concentration was measured using the BCA Gold protein assay (ThermoFisher). Equal protein amounts (25μg ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were sonicated and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, cat.no. 23225) for subsequent analyses.
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bioRxiv - Microbiology 2024Quote: ... Dried proteins were reconstituted in 0.2% SLS and the amount of proteins was determined by bicinchoninic acid protein assay (Thermo Scientific). For tryptic digestion 50 µg protein was incubated in 0.5% SLS and 1 µg of trypsin (Serva ...
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bioRxiv - Cell Biology 2020Quote: ... 5 micromols sodium sulfo-NHS and 5 micromols 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Thermo Fisher #22980) in 10 μL of DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split every 2-3 days through 9 minutes incubation with EDTA 50mM pH=7.4 and 3 minutes of 0.25% trypsin (Gibco).
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bioRxiv - Neuroscience 2021Quote: ... was performed using the Applied Biosystems QuantStudio 3 Real-Time PCR system and analyzed with the QuantStudio 3 Design and Analysis software (v1.5.1,ThermoFisher). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: 3′-desthiobiotin labeling was carried following the manufacturer’s’ guidelines of Pierce™ RNA 3′ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The pbf1 gene of johansen Standard was amplified with the primer pair psbNRO_F 5’-AGCATTGGGAGGCTCATTAC-3’ and psbNRO_R 5’-GGAAACAGCAACCCTAGTCG-3’ and cloned into to pCRTM2.1-TOPO® (Invitrogen). The vector was linearized with HindIII and in vitro transcription was performed according to the suppliers’ protocol ...
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bioRxiv - Microbiology 2020Quote: ... RdRp_rev 5’-CAAATGTTAAAAACACTATTAGCATA-3’ RdRp_probe 5’-FAM-CAGGTGGAACCTCATCAGGAGATGC-TAMRA-3’) and/or TaqMan gene expression assays (Applied Biosystems) for ACTB (Hs99999903_m1) ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 3 µL RT reaction mix consisting of 3 µL of 50 µM random hexamers (Thermo Fisher, cat#48190011), 0.8 µL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Genomics 2021Quote: 3’-desthiobiotin labeling was carried following the manufacturers’ guidelines of Pierce™ RNA 3’ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Immunology 2020Quote: ... and 50 µL of 50 mg/mL 1-ethyl-3-[3-dimethyl-aminopropyl]-carbodiimidehydrochloride (Thermo Fisher Scientific, 22981) were simultaneously added to the reaction tubes ...