Labshake search
Citations for Thermo Fisher :
3551 - 3600 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: 106 HEK cells were plated in 6-well plates and transfected with the indicated plasmids with Lipofectamine 2000™ reagent (Invitrogen). 24 hours after transfection ...
-
bioRxiv - Biophysics 2022Quote: ... gels were functionalized with 0.5 mg/ml solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Thermo Fisher Scientific) prepared in 50 mM HEPES buffer (Santa Cruz Biotechnologies) ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293T/17 cells (ATCC) were seeded at a density of 6×104/cm2 in Eagle’s Minimum Essential Medium (EMEM, Thermofisher, Waltham, MA), containing 10% FBS ...
-
bioRxiv - Developmental Biology 2022Quote: Total protein extracts were obtained by homogenization of pools of 20 6-dpf larvae in ice cold RIPA buffer (ThermoFisher, 89900) and Complete EDTA-free protease inhibitor cocktail (Sigma ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and the second one carrying the mutation to be introduced prepared by PCR amplification of a 6 kb region using genomic DNA of evolved clones as template and high fidelity Phusion polymerase (ThermoFisher Scientific), were co-transformed into naturally competents cells of Ralstonia evolved clones ...
-
bioRxiv - Cell Biology 2022Quote: ... 250.000 HEK293T cells were seeded per p35 plate (pre-coated Corning BioCoat Poly-D-Lysine 35 mm #354467 or uncoated Nunclon multidish 6 well plates, Thermo Scientific #140685 coated with 50 µg/ml Poly-D-Lysine solution (Gibco A38904-01 ...
-
bioRxiv - Cell Biology 2022Quote: ... The transcripts of lysosome biogenesis genes and their transcription factors were amplified from the cDNA in QuantStudio 6 Flex real-time PCR system (Applied Biosystems). As a control 18S rRNA or β-Actin were amplified along with the lysosomal genes ...
-
bioRxiv - Microbiology 2020Quote: ... cells were extensively washed and incubated with secondary antibodies in the presence of 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes) for 45 min at RT ...
-
bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
-
bioRxiv - Developmental Biology 2021Quote: ... A target set of 20 short Alexa647 labelled oligo mouse Dux ViewRNA ISH probe (VB6-3223670-VC, type-6; ThermoFisher Scientific) was designed and used to hybridize to Dux RNA sequence ...
-
bioRxiv - Microbiology 2020Quote: hiPSC-derived macrophages wild type or HPS4-deficient were plated on non-tissue culture treated 6-well plates (Thermo Fisher Scientific) and infected with S ...
-
bioRxiv - Neuroscience 2021Quote: Non-neuronal cell cultures were prepared from postnatal day 0 (P0) C57BL/6 mouse cortices and cultured in Dulbecco’s MEM growth medium (Invitrogen Cat#11960044) supplemented with 10% FBS (Millipore Sigma Cat#F4135) ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed and mounted in media (0.5% N propyl gallate, 70% glycerol, 1M tris pH8.0) containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. No. D1306).
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
-
bioRxiv - Immunology 2020Quote: PBMC (1-2*10^6 cells) and pDC (1-4*10^4) were maintained for 16 h in RPMI1640+10% FBS+ 1% (PS) (Gibco Laboratories) in 96 well round bottom plates ...
-
bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
-
bioRxiv - Bioengineering 2021Quote: Ovaries from 6 to 8 day-old CBA × C57BL/6 (F1) mice were collected and transferred to Leibovitz L-15 media (P/N 11415, Gibco, USA). The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM ...
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
-
bioRxiv - Microbiology 2022Quote: Adenovirus containing the MCMV IE1 gene was generated by transfecting purified pAd5-MCMV_IE1 into 293A cells in 1 well of a 6 well plate using Lipofectamine 3000 (ThermoFisher, Waltham, MA) according to manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
-
bioRxiv - Microbiology 2022Quote: ... L2 fibroblasts were seeded at 1.5 x 106 cells per well in 6-well tissue culture-treated plates containing collagen-coated glass coverslips (Thermo Fisher Scientific) and inoculated with L ...
-
bioRxiv - Molecular Biology 2022Quote: ... 500ng circRNAs per well for 24-well plates (2 μg for 6-well plates) were transfected with lipofectamine MessengerMax (Invitrogen, LMRNA003) on the next day when the cell culture must have >90% viability and be 70% confluent ...
-
bioRxiv - Pathology 2022Quote: ... The iPSCs were continuously cultured and expanded on Geltrex coated 6-well plates with Essential 8 medium (ThermoFisher Scientific, Cat#A1517001). Human iPSC cell lines used in this manuscript ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were centrifuged at 135 x g for 1 minute and resuspended in 6 ml of hepatocyte media (William’s E media (Gibco, 22551-022) with 3% fetal bovine serum (Gibco ...
-
bioRxiv - Cell Biology 2022Quote: ... 6) The enriched fraction was pre-cleared twice by adding 60 μL equilibrated DEPC-treated Protein A Dynabeads (ThermoFisher Scientific 10001D) and nutating at 4°C for 30 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... After extensive washing slides were air-dried and mounted in Gold Antifade containing 4’,6-diamidino-2-phenylindole (DAPI) (P3693; Life Technologies) for imaging ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... All slides were then rinsed three times with PBS-T and twice with 1× PBS and coated with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images were acquired using an Olympus BX63 automated fluorescence microscope equipped with an Olympus DP74 digital camera and evaluated with cellSens Dimension software (Olympus).
-
bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS-T and mounted under Prolong gold fluorescence media with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, P36931).
-
bioRxiv - Neuroscience 2019Quote: ... The slices were incubated separately in wells with PBS and 5 ng/ml DAPI (4′,6-diamidino-2-phenylindole) for 20 min before they were rinsed in PBS and mounted in SlowFade Gold (Life Technologies). Superficial cortical areas of anterior and midcingulate cortex were imaged on a Leica SP8 inverted confocal microscope with a 63x oil immersion objective (numerical aperture = 1.4 ...
-
bioRxiv - Cancer Biology 2019Quote: ... VM-CUB1 and RT-112 cells were seeded at 1 × 105 cells per well in 6-well plates along with siRNA duplexes or miRNAs using Lipofectamine RNAiMAX Reagent (Life Technologies). At 72 hours after transfection ...
-
bioRxiv - Microbiology 2019Quote: Cells were cultured in 6-well plate to 90% confluency and total RNA was extracted by Trizol (Life Technologies, Carlsbad, CA) and RT-qPCR was performed following the instruction of the manufacturer ...
-
bioRxiv - Pathology 2019Quote: ... EB’s were returned to the ultra-low 6 well plate and transferred to Stage II media (DMEM, 15% KnockOut Serum Replacement (KOSR; Thermo Fisher), 1% NEAA ...
-
bioRxiv - Neuroscience 2019Quote: ... Residual genomic DNA was removed from each of 6 biological replicates using the TURBO DNA-free™ Kit (Life Technologies, AM1907) per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
-
bioRxiv - Cell Biology 2019Quote: ... RNA was isolated from whole eyes of 6-month old cep290 mutant and sibling control animals (n = 9 per group) with Trizol (ThermoFisher 15596026). Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 2 µL of 1.25 µM 6-FAM-labeled ADO-22 primer and 1 µl of 100 mM ddTTP (USB-Affymetrix, Cleveland, OH). Fluorescently-labeled cDNA fragments were analyzed by capillary electrophoresis (CE) ...
-
bioRxiv - Neuroscience 2020Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
-
bioRxiv - Neuroscience 2020Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
-
bioRxiv - Immunology 2019Quote: Fabs from rabbits were expressed in 293F cells for 6 days and then affinity purified using a CaptureSelect™ CH1-XL Pre-packed 1 ml Column (ThermoFisher).
-
bioRxiv - Zoology 2020Quote: ... each immunoblot lane represents a cell lysate produced from an entire well of a 6-well cell-culture dish (Thermo Scientific, Nunc). Lyates were diluted with SDS loading dye (final concentration ...
-
bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
-
bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
-
bioRxiv - Molecular Biology 2019Quote: ... cells were seeded on a 6-well plate and transfected with 10 pmol of siControl (negative control #4390843, Ambion Silencer Select), siMCM2 (#s8536 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alexa Fluor 488–conjugated phalloidin (#A12379) and 4’,6-diamidino-2-phenylindole (DAPI) (#1306) were obtained from Invitrogen (Carlsbad, CA, USA). IRDye800CW-labeled anti-rabbit secondary antibody (#926-32211 ...
-
bioRxiv - Neuroscience 2020Quote: ... slides were washed and stained with 4′,6-Diamidin-2-phenylindol (DAPI, 5μg/ml) before mounting coverslips with ProlongTM Gold Antifade reagent (Thermo Fisher Scientific). Images were acquired using a LSM 710 confocal microscope (Zeiss ...
-
bioRxiv - Physiology 2020Quote: ... The cells were transiently transfected for 16-24 hours with either human or mouse orthologs of TRPA1 using Fugene 6 transfection reagents and Opti-MEM (Thermofisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... hiPSCs at ~ 70% confluence (1.5×106 cells / well of a 6-well plate) were incubated in EDTA (Life Technologies #15575-020) for 4 min at room temperature (RT) ...
-
bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
-
bioRxiv - Microbiology 2021Quote: ... samples were unfrozen and aliquots of 6 ml per sample were fixed with Electron Microscopy Sciences 4% Paraformaldehyde (5025999, Fisher Scientific), stained with SYBR™ Gold Nucleic Acid Gel Stain (S11494 ...