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Citations for Thermo Fisher :
3401 - 3450 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Bacterial suspensions were further centrifuged at 3,000 rpm during 30 minutes at 6 °C and resuspended in the same volume of RPMI 1640 broth (Gibco, USA) for intranasal piglet infection ...
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bioRxiv - Microbiology 2024Quote: The protein concentration of gradient fractions 6 and 17 was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher). Ten micrograms of the purified fractions 6 (exosome fraction ...
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bioRxiv - Neuroscience 2024Quote: ... 4 EBs/well were transferred to an Ultra-low attachment 6-well plate (cat#: 07-200-601; Thermo Fisher Scientific) and maintained in Maturation Medium (STEMdiff™ Cerebral Organoid Basal Medium 2 + Supplement E + 100 µg/mL Primocin ...
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bioRxiv - Neuroscience 2024Quote: ... before incubation with DAPI (4’,6-diamidino-2-phenylindole, dilactate, 0.1 µg ml−1 final concentration in PB, Invitrogen D3571) in 0.1 M PB for 10–20 mins at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed 6 times with PBS for 2 min and incubated with Alexa Fluor 568- (1:500 dilution, Invitrogen), Alexa Fluor 488- (1:500 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... For 10 ml of expression tests 107 cells are harvested by centrifugation (1400 rpm for 6 min) and resuspended in RPMI 1640 medium (Invitrogen) containing 0.1 % pluronic at a concentration of 20 x 106 cells /ml ...
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bioRxiv - Molecular Biology 2024Quote: ... 500,000 cells per well were seeded in a 6-well plate and were transfected with Lipofectamine 3000 according to the manufacturer’s protocol (Invitrogen L3000015). For the transfections ...
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bioRxiv - Cell Biology 2024Quote: ... cells were reverse transfected with 1ug plasmids in a 6-well plate using JetOptimus (Polyplus#101000025) or Lipofectamine2000 (Thermofisher#11668019) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded in coverslips treated with poly-L-lysine (10 μg/mL) and grown for 6 days in Neurobasal medium (Gibco) supplemented with 0.05% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... the synthesized cDNA was subjected to real-time PCR analysis using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Synthetic Biology 2024Quote: ... HEK 293T cells were cultured in 6-well plate and transfected at approximately 80% confluency in opti-MEM reduced serum medium (Gibco). Per each well ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear extracts were quantified with Bradford and 2mg of lysate was mixed with 6 µg of antibody and 50 µL of Protein G magnetic beads (Dynabeads, Invitrogen) in 500 µl of buffer C (300 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 100μm lung slices were washed twice in 200 mM HEPES buffer then incubated for 20 min in a staining solution containing: 0.715 μM DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific D1306), 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 μL purified GST-FDL1 protein were incubated at room temperature for 20 min and loaded on a 6% DNA retardation gel (Life Technologies) before transfer to a nylon membrane ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed with specific primers (Table S1) using the QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Microbiology 2020Quote: Total RNA from SARS-CoV-2 USA-WA1/2020 or rSARS-CoV-2 infected (MOI=0.01) Vero E6 cells (106 cells/well, 6-well plate format) was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: HEK293F cells grown in a 6-well plate were transiently transfected with CFTR constructs labelled with C-terminal eGFP tag using Lipofectamine 3000 (Thermo Fisher) in Opti-MEM (GIBCO ...
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bioRxiv - Cancer Biology 2021Quote: ... Experiments were conducted by plating cells in a 6 well plate for next day transfection when cells where 70-80% confluent using Lipofectamine RNAiMax (Invitrogen; 13778100). A final concentration of (20 pmoL ...
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bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
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bioRxiv - Cancer Biology 2021Quote: Feeder-free iPS cell culture was maintained on matrigel coated 6-well plates and routinely passaged with 1 ml accutase or 0.5 mM EDTA (Thermo Fisher Scientific). To enhance single cell survival ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Cell Biology 2021Quote: ... at 1000xg for 15 min followed by 10,000xg for 15 min in 500 ml vessels using a fixed angle FIBERlite F14-6×500 y rotor (Thermo Fisher Scientific). The supernatant was centrifuged at 100,000xg (28,000 RPM ...
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bioRxiv - Microbiology 2020Quote: ... or by incubating at 37°C for 48h and subsequently adding 6 µM Hoechst 33342 nucleic acid stain (Thermo Fisher Scientific) as a background stain for high content imaging analysis ...
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bioRxiv - Cell Biology 2020Quote: ... 0.3×106 control and senescent AML12 cells were seeded in each well of 6-well plate for cell replication analysis and counted (using Countess™ II Automated Cell Counter, ThermoFisher) at every other day for three days as shown in figure 1c.
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bioRxiv - Cell Biology 2019Quote: ... Transient expression was obtained by electroporation performed 24 hours before the experiment using the Neon electroporation system (Thermo Fisher 6 Scientific). For each transfection ...
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bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
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bioRxiv - Cell Biology 2020Quote: ... A gradient consisting of 0% to 40% B in A (A: 0.1% formic acid [Fisher Scientific, A117], 6% acetonitrile [Fisher Scientific, A955] ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 were measured according to the manufacturer’s instructions in plasma and peritoneal lavage supernatant using Novex ELISA Kits (Thermofisher Scientific Ltd.).
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bioRxiv - Developmental Biology 2021Quote: ... and gene primers (Supplementary table 1) were used to perform qPCR on QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher) and normalized to housekeeping gene Tbp ...
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bioRxiv - Cell Biology 2020Quote: Quantitative PCR was done on the QuantStudio 6 Flex with the QuantStudio Real-Time PCR software using SYBR green PCR master mix (Applied BioSystems). Primers (see Appendix S2 for primer sequences ...
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bioRxiv - Microbiology 2020Quote: ... Excess protein was blotted away for 6 seconds before being plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Scientific). Frozen grids were imaged in a Titan Krios (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Primary neurons were plated onto poly-L-lysine-coated coverslips for 6-well plates and cultured in MEM medium (Thermo Fisher) containing 2% B27 and 5% FCS ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2×105 cells were plated per well of a 6-well plate and 18 hours later cells in 1750μl of Opti-MEM (31985070, Thermo Fisher Scientific) were transfected with 50nM of siRNA targeting HAND2 (s18133 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A 3’ Venus tag was added by recombining the Nvsix3/6 coding sequence into pSPE3-R-Venus destination vector using the Gateway LR cloning reaction (ThermoFisher Scientific). mRNA synthesized and injected at 300ng/µL using previously described methods 61 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher Scientific) in OptiMEM (400 μL ...
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bioRxiv - Cancer Biology 2022Quote: ... Using a 6 to 30% gradient over 84 mins peptides were separated and analyzed using Thermo Fusion Lumos mass spectrometer (Thermo Scientific). Following parameters were used- MS1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Over-expression vectors were prepared by cloning the PLAGL2 coding sequence into BII-ChBtW and IEC6 cells transfected in 6 well plates with 1600 ng of this plasmid and 400 ng of pCMV-hyPBase using Lipofectamine 2000 (ThermoFisher Inc.). Cells were select 48 hours after transfection with medium containing 4 µg/ml Blasticidin and selected for 5 days ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 and 6 were directly cloned into pCR(tm)2.1-TOPO® using the TOPO® TA Cloning® Kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: DR-U2OS and -HCT116 cells and EJ-U2OS and -HCT116 reporter cells were plated in 6 well plates and transfected with 5 μL Lipofectamine RNAiMAX transfection reagent (Thermo Fisher) and siRNAs (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Approximately 4 x 105 HEK293T cells in a 6-well plate were transfected with 250 ng/well of pMT107-octa-6 His-ubiquitin or 200 ng/well of pcDNA3-2x-6His-ubiquitin constructs using 50 µl/well Opti-MEM (Gibco; 31985062) and 2 µl/well of FuGENE 6 Transfection reagent (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction mixtures containing SYBR Green were composed following the manufacturer’s protocol and then CT values were obtained using a qPCR platform (QuantStudio 6 Flex Real-Time PCR System, ThermoFisher Scientific). Real-time PCR data was monitored using QuantStudio 6 Design and Analysis Software Version 2.3 ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Each cDNA sample was diluted 1:25 and real-time quantitative PCR (qPCR) was performed in duplicate using miRNA-specific primers/probe on a Quant 6 studio Real Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 60 WT and MafAS64F/+ islets from at least 6 female and male mice were analyzed with the ratiometric calcium indicator fura-2-acetoxymethylester (Fura-2 AM) (Life Technologies). Islets were maintained in 5 mM glucose for 30 min prior to measuring 11 mM glucose-induced calcium oscillations and depolarization-activated calcium influx with 30 mM KCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Aggregation was initiated separately at a concentration of 6 μM for each construct in vitro by addition of AcTEV™ protease (Invitrogen), as previously described for mEx1-Q51 (Shahmoradian et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 × 105 of the sorted cell types and the unsorted mixture were seeded and cultured in 6-well plates coated with fibronectin bovine (LIFE Technologies). Medium composition was equivalent to the 3D differentiation.
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Cell Biology 2022Quote: ... the tryptic peptides from top and bottom of Stentor cell were labeled isobarically with the 6-plexed tandem mass tag (TMT) reagents (ThermoFisher Scientific): TMT126 (top) ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were seeded into 6-well plates (one million per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 25 mM unlabeled glucose ...
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bioRxiv - Genomics 2020Quote: ... 10 ng of the library were transfected into 250 000 HEK293 cells in one well of a 6-well plate using Lipofectamine 2000 (11668027, ThermoFisher Scientific) and OPTIMEM I Reduced Serum Medium (31985-047 ...