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Citations for Thermo Fisher :
3801 - 3850 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... Growth medium on HEK293T cells at 90% confluency in 6 well plates was replaced with Opti-MEM reduced serum medium (Thermo Fisher) and cells in each well were transfected with 250ng each of 8 pDUAL reverse genetics plasmids ...
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bioRxiv - Systems Biology 2023Quote: The PCR products (6 µl per well) were confirmed through 96-well E-gel with SYBR (Thermo Fisher, Catalog no G720801) using 25 µl of loading buffer (Thermo Fisher ...
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bioRxiv - Systems Biology 2023Quote: The PCR products (6 µl per well) were confirmed through 96-well E-gel with SYBR (Thermo Fisher, Catalog no G720801) using 25 µl of loading buffer (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... mNat2 mRNA expression levels were determined by quantitative polymerase chain reaction (qPCR) using the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). The cDNA was amplified using primers for mNat2 variant 2 and mGAPDH (see Supplementary Table S5 for details ...
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bioRxiv - Biochemistry 2023Quote: ... Bacmid DNA containing the construct of interest was transfected into 2mL of Sf9 cells at a density of 1×106 cells/mL on a 6-well plate using ExpiFectamine Sf Transfection Reagent (Life Technologies) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... SVG-A Notch2-HaloTag cells were transfected in 6 well plate format with 2 µg of DNA per well using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR assays were performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA). The TaqMan Universal PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative reverse-transcription-PCR (qRT-PCR) was performed on a QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems A43182) in 384-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... The expression levels of selected genes were determined using qRT-PCR (QuantStudio 6, ThemoFisher Scientific) using the SYBR Green Real-time PCR master mix (Thermo Scientific). The sequence of the primers used for the gene expression analysis was given in an appendix.
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bioRxiv - Cancer Biology 2023Quote: ... The B6-LSL-Cas9-eGFP keratinocytes were transiently transfected with 1μg of a CRE-expressing plasmid in 6-wells using Lipofectamine 2000 (Invitrogen; 11668-027) to activate Cas9-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were transfected with PNKP-FLAG (WT and mutants) expressing vector (pcDNA3) in 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged every 5-6 days as small clumps by dissociation with a buffer containing 1 mg per ml Collagenase IV (ThermoFisher Scientific), 0.025% Trypsin (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed in technical quadruplets on a QuantStudio 6 Flex Real-Time 384-well PCR System (Applied Biosystems, 4485694) using PowerUp SYBR Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and then starved in 50 µl EMEM-Serum free medium (antibiotic free) in presence or absence of a protein kinase inhibitor (PKI) (6-22amide, 50 nM, GIBCO BRL) for 3 hours ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no. 150239, Thermo Scientific) and then cultured at 22°C until sexual maturity ...
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bioRxiv - Microbiology 2023Quote: Primary human macrophages were generated by differentiating blood-derived CD14+ monocytes for 6 days in Roswell Park Memorial Institute (RPMI)-1640 medium (ThermoFisher Scientific) containing 10% HyClone FBS (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... cDNA from samples was mixed with QuantiTect probe PCR master mix and gene-specific primers to run on a QuantStudio 6 Flex real-time cycler (Applied Biosystems). To generate a control for the amplification reaction ...
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bioRxiv - Bioengineering 2023Quote: ... reprogramming was continued in E8 medium and from Day 6 to Day 18 in E7 medium (E6 medium, cat. A1516401, Thermo Fisher Scientific + 100ng ml-1 bFGF ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µg of plasmid and 6 µg of PEI were diluted in 0.5 ml of Opti-MEM™ Reduced serum medium (Gibco™) per single well ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μg of DNA and 6 μg of PEI were diluted in 0.25 ml of Opti-MEM™ Reduced serum medium (Gibco™), incubated for 30 minutes at room temperature and added dropwise to plates containing cells ...
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bioRxiv - Bioengineering 2023Quote: HEK293 cells were seeded on a 6-well plate (2×105 cells per well) and were cultured in low glucose Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific) supplemented with 10% FBS and 1% sodium pyruvate ...
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bioRxiv - Bioengineering 2023Quote: ... the hydrogel is first activated with sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH, Pierce, Thermo Fisher Scientific, Waltham, MA). Here the remaining washing buffer around the gel edge is removed to ensure the coating solution can be held on top of the gel ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.6 µm particle size column mounted in a Vanquish LC coupled to an Orbitrap ID-X Tribrid mass spectrometer (ThermoFisher Scientific). The mobile phases and chromatographic gradients used are described in Supplementary Table S6 ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 ng of cDNA was used to perform the qRT-PCR reaction using SYBR green PCR master mix on an ABI Quant Studio 6 Flex Real-Time PCR System (Applied Biosystems). The housekeeping genes GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, Carlsbad, CA, USA). Cells were viewed using an Olympus IX81 or Olympus BX53 epifluorescence microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 x 106 KELLY or 5 x 105 TET21N cells were transfected on a 6-well plate using 7.5 µl Lipofectamine RNAiMAX (Thermo Fisher Scientific) and 50 pmol siRNA according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are cultured for 3 days in a 6-well plate before they are gently detached with 10-min incubation in an enzyme-free cell dissociation buffer (Gibco, 13151014). These detached cells are then replated on ECM-coated substrates and imaged after 6 hrs.
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bioRxiv - Immunology 2023Quote: Spleen cells from CD45.1+ CD45.2- male and CD45.1- CD45.2+ female C57BL/6 wild type mice were harvested as described above and stained with Cell Trace Violet cell proliferation kit (Molecular Probes) in PBS at different concentration ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed in MicroAmp optical 384-well plates using the QuantStudio 6 Flex RT-PCR system (Thermo Fisher). The default thermocycling program was used (95ºC for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: Cell sizes of mouse and rat embryonic molars were determined by staining 6 µm thick histological sections with DiI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... washed 1x with PBS and plated at 200,000 cells per well in 6-well plates coated with matrigel in iMGL media [DMEM/F12 (Thermo Fisher Scientific), ITS-G (2% v/v ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were seeded at 5·105 cells/ml in a 6-well plate in a total volume of 2 ml of Sf-900-II SFM media (10902088, ThermoFisher Scientific). Bacmids (see above ...
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bioRxiv - Biochemistry 2023Quote: ... cells from 100 mL of culture were resuspended and chemically lysed for 30 min in 6 mL of B-PER II (Thermo Fisher) diluted to 1x with TEMB buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... jaw specimen undergone silver deposition and gold enhancement was casted in 6 % low melting point agarose (TopVision, ref R0801, Thermo Scientific) in 1xPBS and sectioned to 100 µm sections with vibratome (Microm) ...
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) was completed using the SYBR green method carried out on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using LabTaq Green Hi Rox (Labtech) ...
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bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... sections were counterstained with 1 mg/mL of 4’,6-diamidino-2-phenylindole (DAPI) and 0.1 nmol units/ml of Phalloidin ATTO647N and mounted with Fluoromount-G (Thermo Fisher Scientific). The slides were acquired using a confocal (Leica SP8 ...
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bioRxiv - Molecular Biology 2023Quote: ... and size exclusion chromatography (SEC) using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was propagated in C6/36 Aedes albopictus cell line at a multiplicity of infection (MOI) of 0.01 and cultured for 6 days in Leibovitz’s L-15 medium (Thermo Fisher Scientific, 11415064) supplemented with 0.3% tryptose phosphate broth (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... After 3 washes cells were stained with a combination of Alexa Fluor secondary antibodies and 4′,6-Diamidino-2-Phenylindole (DAPI; Molecular Probes) for 1 h at room temperature in the dark ...
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bioRxiv - Bioengineering 2023Quote: ... Sigma-Aldrich 7722-84-1) and then slowly injecting 0.03 % w/v sodium dithionite (ACROS Organics 7775-14-6, in PBS) using a syringe pump (10 µL min−1 ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCR was performed using the ChamQ Universal qPCR Master Mix (Vazyme Biotech) on the QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems). The relative transcript level was calculated by the ΔΔCT comparative quantification method (50 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The 6-well plates with prepared corneas were placed on a rocker platform (Figure 40) inside a CO2 incubator (Heracell, Thermo Fisher) and incubated with 5% CO2 at 37°C.
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bioRxiv - Bioengineering 2023Quote: ... to each well we add 200 μL of 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat. No. D3571) solution in DI water ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons (100000 cells/ well of a 6-well) were plated onto poly-L-lysine-coated coverslips and grown in modified Eagle medium (MEM; Thermo Fisher) supplemented with 5% FCS and 2% B-27 (Gibco ...