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Citations for Thermo Fisher :
3751 - 3800 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and these values were re-calibrated based on measurements of NIST-certified polystyrene beads (2 µm and 6 µm in diameter, Duke Standards, Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... BA disks were prepared by transferring 10 μL of preheated 500 mg/mL BA in dimethyl sulfoxide (DMSO) stock to blank antimicrobial disks (6 mm, Fisher Scientific). MH plates were incubated at 37 °C for 48 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and purity (260/280 absorbance ratio of approximately 1.6–2.0 and 260/230 absorbance ratio of approximately 1.8–2.4) confirmed with a NanoDrop spectrophotometer (Thermo Fisher Scientific, MA, USA). Sufficient strand lengths (80% > 40 Kbp length ...
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bioRxiv - Genetics 2024Quote: ... 25 μg of peptides per condition were labelled with isobaric tags by incubation for 1 hour at RT (TMT 6-plex and 10-plex, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... to a final volume of 200 μL and then incubated 90 min at 37°C with 6 μL of RNase cocktail (Ambion, AM2286), followed by 150 minutes at 55°C with Proteinase K (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... 10 min at 95 °C followed by 40 cycles of 10 sec at 95 °C and 40 sec at 60 °C using the Quant 6 real-time PCR instrument (Applied Biosystems). Samples were considered positive for Giardia spp ...
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bioRxiv - Immunology 2024Quote: ... Raji (from DSMZ) and NALM-6 cells (kindly provided by Adolfo Cavalié, Saarland University) were cultured in RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal calf serum (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... non-sun-exposed skin as previously described(13) and used at passages 2-6 and grown in Epilife medium (Gibco, #MEPI500CA) with added human keratinocyte growth supplement (10ul/ml medium) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.12 x 106 COS-7 cells were seeded into each well of a 6 well plate and transfected with 1 μg total DNA using Lipofectamine p3000 (ThermoFisher Scientific). Cells were rinsed in PBS 5 hours post transfection ...
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bioRxiv - Immunology 2023Quote: ... All samples were assayed using the 7300HT or QuantStudio 6 Fast Real-Time PCR System and analyzed using Fast System Software (Applied Biosystems). All real-time PCR data was normalized to the level of GAPDH mRNA ...
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bioRxiv - Bioengineering 2024Quote: A volume of 4 μl of Cy5-labeled DO structures at 50 nM concentration were mixed with 6 μl R Buffer solution (Neon kits-MPK1096 Thermo Fisher). DO structures in R buffer were then immediately subjected to electroporation using the Neon Transfection system (MPK5000 ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs with the initial passage number of 24 were cultured in a 6-well plate coated with 0.5 mg Matrigel™ (354230, Fisher Scientific) in complete mTeSR plus medium (100-0276 ...
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bioRxiv - Cell Biology 2024Quote: ... E0771 cells were harvested by trypsinization and cultured at a density of 106 cells/well in 6-well NunclonTM SpheraTM low-adhesion plates (ThermoFisher, 174932) to prevent substrate attachment ...
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bioRxiv - Biochemistry 2024Quote: ... ExpiCHO-S cells were grown to a density of 6 x 106 cells/mL and transfected using the ExpiFectamine CHO Transfection Kit (ThermoFisher Scientific) and expression carried out for 7-10 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Human embryonic stem cell line H9 was cultured on the 6-well plate coated with the Vitronectin (5 μg/ml) and in the Essential 8 Flex medium (both Thermo Fisher). Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissue specimens were stained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min and mounted in VECTASHIELD Vibrance Antifade Mounting Medium (ThermoFisher Scientific). Akoya Biosciences’ PhenoImager HT (formerly known as Vectra Polaris Automated Quantitative Pathology Imaging System ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were reversed transfected with 1 pmol (96-well) or 25 pmol (6-well) siRNA using Lipofectamine RNAiMAX (Life Technologies, 13778150) and Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 10 nM of siRNA sequences (6-well plates) by using the Lipofectamine RNAiMax system (Thermo Fisher Scientific). Hypoxia was performed as previously described (24) ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR assays were performed in 20 μL reaction volumes using a QuantStudio 6 flex Real-time PCR system (Applied Biosystems). The primers used are listed in Supplemental Table S1.
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bioRxiv - Immunology 2023Quote: ... Invitrogen E-Gel EX Agarose Gels 4%, and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, D1306) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were detected and cover slips were attached using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) containing Prolong Diamond Anti-Fade (Life Technologies). Slides were imaged on a Keyence BX-X810 microscope.
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bioRxiv - Neuroscience 2023Quote: ... HBMECs were seeded in 6-well plates and at 75% confluency were stained for 20 min at RT using a crystal violet (Fisher Scientific) fixing (37% formaldehyde ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated from cultured mouse neurons treated with xcTauOs (250 nM total tau) or vehicle for 6 hours using the mirVana isolation kit (Invitrogen, AM1560). RNA integrity was evaluated by capillary electrophoresis using an Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2023Quote: ... A final wash with PBS was performed before mounting the cells in ProLong® Gold Antifade Mounting medium with DAPI (4’,6-diamidine-2-phenylindole)(Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... and stained the same day of the experiment with the fluorescent dye 5(6)-TAMRA/SE™ (Thermo Fisher Scientific, C1171) at a final concentration of 1 μM (as in Wehrl et al ...
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bioRxiv - Physiology 2023Quote: ... 36 hours upon transfection DMEM-F12 medium in 6-well plates was replaced with fresh DMEM/F-12 medium without phenol red (Gibco #21041025). 48 hours post transfection ...
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bioRxiv - Cell Biology 2023Quote: N2A cells were seeded at 300,000 cells per well in a 6 well plate 24 hours before transfection using Lipofectamine 3000 (Thermo Fisher Scientific). Transfections were performed in accordance with the Lipofectamine 3000 protocol ...
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bioRxiv - Plant Biology 2023Quote: Heat-stress (HS)/LaCl3 experiments: 6-day old cells (20 mL in 100 mL flask) were treated with LaCl3 (Thermo Scientific) (750 µM ...
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bioRxiv - Genetics 2023Quote: Whole cell extracts of 2D cultures from one well of a 6-well plate were prepared using RIPA Lysis and Extraction Buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Synthetic cDNA encoding a self-cleaving polyprotein Bcl-6.t2A.Blc-xL to express the germinal center B cell-associated transcription factors Bcl-6 and Bcl-xL was synthesized (B6L; Integrated DNA Technologies) and cloned in pENTR/D-TOPO (Thermo Fisher), generating pENTR.B6L (35 ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Immunology 2023Quote: ... a total of 6 – 9 consecutive 10-µm coronal sections were taken from of 4 – 6 equally distanced regions along the length of the spinal cord using a Shandon Finesse 325 microtome (Thermo Scientific). Sections were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The grids were blotted with filter paper for 6 s and immediately plunged into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) at 4 °C and 95% humidity ...
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bioRxiv - Neuroscience 2023Quote: ... 500,000 cells were plated in 6-well dishes and 1 μg of DNA was transfected when cells reached 75-85% confluency with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... PBS was replaced with a 1:6 (weight: volume) solution of 0.5 M acetic acid (984303; Thermo Fisher, Waltham, MA, USA) with 1 mg/ml pepsin (20343 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg plasmid DNA per well was transfected into cells for 16 h at a confluency of 60% in 6-well plates using Lipofectamine 3000 (Invitrogen, L3000001) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... anti-mouse IL-1β biotin conjugate (13-7112-81), and anti-mouse IL-6 (MP5-20F3, 14-7061-85) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by medium-speed sedimentation at 10,000xg for 15 min using a fixed angle FIBERlite F14-6 x 500 y rotor (Thermo Fisher Scientific). The supernatant fraction was then centrifuged at 32,000 RPM for 1.25 h in a SW32 rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... reactions were performed using HOT FIREPOL EvaGreen Supermix (Solis BioDyne) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Every sample was analyzed in triplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 6 Flex). For testing of U7 snRNA level in U7 KD cells ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Immunology 2023Quote: ... and 1 × 10^6 were processed using Click-iT EdU Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific Cat. #C10418). The other 1 × 10^6 were subjected to anti-CD3 crosslinking and pERK staining as described below ...
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed using the PTFE filters and resuspended in 1x PBS or further biotinylated with 6 mg/ml EZ-link™ Sulfo-NHS-SS-biotin (#21331; ThermoFisher). Streptavidin conjugated to either AlexaFluor-488 or -568 was then added for 1h RT incubation ...
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bioRxiv - Immunology 2023Quote: ... Monocyte-derived macrophages (MDM) were generated by seeding three million CD14+ cells into one 6-well plate (NuncTM Thermo Fisher Scientific) in IMDM (Thermo Fisher Scientific ...