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Citations for Thermo Fisher :
3451 - 3500 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 1.5 million cardiomyocytes were re-plated per well of a 6-well plate on Day 20 in Cardiomyocyte Maintenance Media [500 mL DMEM without glucose (A14430-01, ThermoFisher Scientific), 50 mL FBS (S1200-500 ...
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bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...
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bioRxiv - Immunology 2021Quote: ... was added to each sample for the PCR reaction and run on the QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific). The value of STAT3 Exons 20/21 and Introns 20/21 was calculated as 2ΔCt(GAPDH) ...
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bioRxiv - Immunology 2021Quote: ... the barcoded samples were combined and diluted to 6 x 106 cells / 800 μl CyFACS per well in Nunc 96 DeepWellTM polystyrene plates (Thermo Fisher). Cells were blocked with mouse (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting suspension with all nuclei was stained with the fluorescent DNA intercalant marker DAPI (4’-6-diamidino-2-phenylindole dihydrochloride, Invitrogen, USA) diluted 1:20 from a stock solution of 20 mg/L and filled with PBS 0.1M to known volume ...
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bioRxiv - Zoology 2020Quote: ... Tissues were cut into small pieces with a scalpel and washed twice in ADPBS containing antibiotics prior to placing 4-6 tissue pieces into a 25 cm2 plug-seal tissue culture treated flask (BioLite, Thermo Scientific) containing 1.5 mL establishment medium to cover the tissue pieces ...
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bioRxiv - Molecular Biology 2019Quote: ... All RT products were used in total of 300 ul (50 uL × 6) PCR reaction with Phusion polymerase (Thermo Fisher Scientific) and gene specific primers with Fluidigm mmPCR adaptor sequences51 ...
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Genomics 2020Quote: ... and approximately 1.6 × 106 C57BL/6 ESCs were transfected with 15 μg of linearized vector using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... Differentiation experiments were carried out for 10 days on gelatin-coated 6-well plates by transitioning cells in Dulbecco modified Eagle medium (DMEM, ThermoFisher Scientific) with 10% of fetal bovine serum ...
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bioRxiv - Developmental Biology 2019Quote: ... blastocysts were collected from uterine horns and put on culture for 3-6 days in ES derivation medium composed of GlutaMAX/DMEM (Gibco, 31966), 15% FBS (Biowest) ...
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bioRxiv - Neuroscience 2019Quote: ... The medium was changed every day and the cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Developmental Biology 2019Quote: ... then transferred into agarose-coated 6-well plates containing explant medium - comprised of Dulbecco’s modified eagle medium with nutrient mixture F-12 (Gibco DMEM/F12) containing 2.5mM L- Glutamine ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Physiology 2019Quote: ... 5 muscle samples 100 μg each corresponding to 5 different age groups and one separately prepared master reference sample were labeled with 6-plex tandem mass spectrometry tags using a standard TMT labeling protocol (Thermo Fisher). 200 femtomole of bacterial beta-galactosidase digest (SCIEX ...
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Differentiation to ECs were initiated by treating the hiPSCs with 6 μM CHIR99021 in RPMI-B27 without insulin media (Life Technologies) for 2 days ...
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bioRxiv - Bioengineering 2019Quote: B-cells were isolated from two C57BL/6 mice via MACS depletion (CD43 microbeads, 130-049-801 MACS) and labelled with Cell Trace Violet (ThermoFisher, C34557) or Cell Trace Far Red (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... they were incubated with donkey anti-chicken rhodamine and donkey anti-rabbit Alexa 488 secondary antibodies for 1.5 hours and then with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen Life Technologies) (1:20 ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293F cells were cultured at 37 °C with 6% CO2 and 70% humidity in Free Style 293 medium (Thermo Fisher Scientific) supplemented with 1% fetal bovine serum (FBS).
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bioRxiv - Immunology 2019Quote: ... DRGs inside vertebral column and the depilated hairy skin from PFA-perfused animals (6-12 weeks) were postfixed with 4% PFA for 1 hr or Zamboni fixative (Fisher Scientific) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time PCR (qPCR) was performed on a QuantStudio 6 Flex (Thermo Fischer Scientific) with Fast SYBR Green Master Mix (Life Technologies). Thermocycling parameters were defined as 95°C for 20 sec followed by 40 cycles of 95°C for 1 sec and 60°C for 20 sec ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Neuroscience 2019Quote: ... To preserve a more intact hair cell intracellular milieu we also performed perforated-patch recordings using an internal solution with gramicidin (40-50 μg/ml; Fig. 5A and 6) plus a fluorescent dye (Alexa Fluor 488 Hydrazide; ThermoFisher Scientific). Hair cells containing dye in their cytoplasm were excluded from analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.3 million cells were platted in a 6 well dish and cell proliferation was measured using trypan blue (Invitrogen, Carlsbad, CA) staining.
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bioRxiv - Biochemistry 2021Quote: ... The grids were blotted for 6∼7 s at 100 % humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher). The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the following thermal cycling conditions ...
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bioRxiv - Cell Biology 2021Quote: ... BEAS-2B cells (2.5 × 105/well in 6-well plate) were transfected with CRISPR-Cas9 plasmids by Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 16 EBs/well were plated in a 6-well tissue culture polystyrene plated coated with Matrigel™ in hematopoietic myeloid medium (HIM) consisting of 2mM GlutaMax (ThermoFisher), 55 μM beta-mercaptoethanol ...
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bioRxiv - Genomics 2021Quote: ... Cells (100000 cells/well in 6-well plates) were reverse-transfected in antibiotic-free medium by 25 nM miRNA mimic (ThermoFisher, 4464066) or control mimics (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: RBDwt was conjugated to CuMVTT using the cross-linker succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, MA, USA) at 7.5 molar excess to CuMVTT for 30 min at 25 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make) with the QuantStudio 6 Flex Real-Time PCR system (Life Technologies). The mRNA levels of ß-actin were used as an internal control to normalize the mRNA levels of Hsp70s ...
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bioRxiv - Biophysics 2021Quote: ... cells were transiently transfected with 4 μg mouse Piezo1-pIRES-EGFP in pcDNA3.1(+) or 4 μg YFP 40-48 hours before recording in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: Each gel electrophoresis reaction used 6 µL of unbound B56 protein-antibody complex and 2 µL of 4x SDS-PAGE sample buffer (Thermo Fisher). After heating the samples to 95 C for 10 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol-free DMEM + 10% FBS + 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR reactions were performed in triplicate on QuantStudio 6 Flex Real-Time PCR Systems (Cat#4485694, Applied Biosystems, Waltham, USA) using the TaqMan Fast Advanced Master Mix (Cat#4444963 ...
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bioRxiv - Bioengineering 2020Quote: ... and subjected to heat-induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, Fisher Scientific International, Inc., Pittsburgh, MA) at >80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Eluates were heated to 65°C for at least 6 h to reverse the formaldehyde cross-linking and then treated with proteinase K (Life Technologies). DNA fragments were isolated using a DNA purification kit (Qiagen) ...
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bioRxiv - Biophysics 2021Quote: ... accessed 6/18/2019) and the pepsin amino acid sequence using Proteome Discoverer software (version 1.3, SEQUEST algorithm, Thermo Fisher Scientific) or Byonic software (Protein Metrics ...
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bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
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bioRxiv - Neuroscience 2021Quote: ... and placed in the QuantStudio 6 Pro Real-Time PCR system (all reagents for RT-qPCR were purchased from ThermoFisher Scientific). The PCR cycle protocol was ...
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bioRxiv - Neuroscience 2020Quote: Spinal cord tissue from stage HH 25-26 (Day 5-6) chick embryos (Venkateshwara hatcheries Ltd.) were dissected out in L-15 medium (Gibco, HiMedia) with added 1x penstrep (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: Neuro-2a cells seeded in 6-well plates (6×105/well) were transfected with 100 nM circTulp4-specific or negative-control siRNA by using Lipofectamine® RNAiMAX (Invitrogen). At 24 h post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... with specific primer pairs (table 1) were used for all qPCR assays and analyzed via QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Technical and experimental triplicates were performed for all samples ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed 3 x 5 min with PBS-TX and once with 0.1 M phosphate buffer pH 7.4 (0.08 M sodium phosphate dibasic and 0.02 M sodium phosphate monobasic) prior to coverslipping with ProLong Gold antifade reagent with 4’,6-diamidino-2-phenylindole [DAPI] (Invitrogen, P36931).