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Citations for Thermo Fisher :
3351 - 3400 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Cells were replated at desired ratio (3.75 x 105 cells per well/of a 6-well plate) on Geltrex-coated wells in neuronal induction media: DMEM-F12 (Gibco) containing 1x N2 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time qRT-PCR was performed on the QuantStudio 6 Flex thermocycler using PowerUp SYBR green master mix (ThermoFisher Scientific). All reactions were prepared according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 10 to 20 ovaries from 3-6 day-old flies using TRizol (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... live cells were imaged within 6-well plates at 20x magnification using an EVOS FL monochrome microscope (Invitrogen, catalogue #AMF4300) running Invitrogen image acquisition software ...
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bioRxiv - Biochemistry 2024Quote: Hepa1-6 cells (mouse hepatoma; ATCC; CRL-1830) were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS) (Gibco) and maintained at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Bioengineering 2023Quote: ... in blocking buffer prior to a final incubation with 4′,6-diamidino-2-phenylindole (DAPI) or anti-phalloidin for F-actin (Invitrogen) at 25 °C ...
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bioRxiv - Neuroscience 2023Quote: One million cortical cells and one million striatal cells were suspended in 6 mL D-minimum essential medium (DMEM, GIBCO) with 10% foetal bovine serum (DMEM+) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... following manufacturer’s protocols and gene expression quantified via Applied Biosystems Quant Studio 6/7 Real-Time PCR System (Applied Biosystems), for TaqMan (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cell Biology 2023Quote: 6-well plates were coated with a solid base of 1 mL of agar (0.8% in complete DMEM) (Thermo Fisher). On top of this solid base ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected in 6-well plates with 2 µg of the indicated constructs in the presence of Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cancer cell lines with z stable Cas9 overexpression introduced by lentiviral infection were seeded in 6-cm dishes and transfected with gRNA mixes using Lipofectamine MessengerMax (Invitrogen), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The A1 mouse ES WT and Nsd1-KO lines 73 (background C57BL/6 × 129S4/ SvJae F1) were obtained from David Allis lab and maintained on gelatin-coated plates in Knockout DMEM (Gibco) supplemented with 15% ES-cell-qualified FBS (Gemini) ...
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bioRxiv - Molecular Biology 2023Quote: One day after seeding 106 U2OS cells in 6-well plate, small interfering RNAs (siRNA, 20μM) were delivered using Lipofectamine® RNAi-MAX Reagent (Invitrogen) according to the supplier’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL of this cell suspension was plated into 3 wells on a bottom layer of 0.5% select agar in RPMI+10% FBS in 6-well non-tissue culture treated dishes (Thermo Scientific). For soft agar inhibitor experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... Two hundred and fifty thousand NIH3T3 cells plated in a 6-well plate were transfected in the presence of Lipofectamine 2000 (Invitrogen) (per well 2 µg DNA/4 µl Lipofectamine 2000 in 400 µl Opti-MEM medium ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with 0.1% Triton X-100 in PBS supplemented with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml) (Invitrogen, # 62248) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and envelope (pMD2.G; 0.5 µg) plasmids using either FuGENE 6 or Lipofectamine 3000 transfection reagent (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were seeded in a 6-well plate overnight before being transfected with 2.5 μM of siRNAs using Lipofectamine RNAiMAX transfection reagent (Invitrogen, USA) according to the manufacturers instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Hepa1-6 (CRL-1830, ATCC), HEK293T (ACS-4500, ATCC), 3T3L1 (CL-173, ATCC) cells were cultured in DMEM medium (C11995500BT, Gibco) supplemented with fetal bovine serum (04-400-1A ...
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bioRxiv - Genomics 2024Quote: ... the fibroblasts were thawed and plated at a density of 2.5×105 cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Microbiology 2024Quote: Brachyspira pilosicoli strain P43/6/78 (ATCC #51139) was grown in Brain Heart Infusion (BHI) broth (Thermo Fisher Scientific, #CM1135B) containing 10% heat-inactivated fetal bovine serum and 0.2% glucose solution for four days in an anaerobic chamber (Coy Lab Products ...
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bioRxiv - Cell Biology 2023Quote: ... the NPC line used is designated MGH2046-RC1 and is derived from an individual in her 50s with FTD and carrier of the tau-P301L autosomal dominant mutation (c.C1907T, rs63751273).31,188 NPCs were cultured in 6-well (Fisher Scientific Corning) or black 96-well clear bottom (Fisher Scientific Corning ...
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bioRxiv - Cell Biology 2024Quote: ... cells were reverse transfected with 1ug plasmids in a 6-well plate using JetOptimus (Polyplus#101000025) or Lipofectamine2000 (Thermofisher#11668019) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded in coverslips treated with poly-L-lysine (10 μg/mL) and grown for 6 days in Neurobasal medium (Gibco) supplemented with 0.05% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... the synthesized cDNA was subjected to real-time PCR analysis using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Physiology 2024Quote: ... CHX-treated cells were harvested at different time points (0, 2, 4 and 6 hours) and processed for immunoblotting with anti-LSD1 (Invitrogen) and anti-RCOR1 (Abcam ...
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bioRxiv - Biophysics 2024Quote: ... DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) in phosphate buffered saline (PBS) and cells were mounted with ProLong Gold Antifade reagent (Invitrogen).
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bioRxiv - Biophysics 2024Quote: ... together with 0.5 μg psPAX2 packaging vector and 0.5 μg pMD2.G envelope vector DNA were co-transfected to 90% confluence HEK293T cells in a 6 cm petri dish using Lipofectamine 3000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Target cells were infected with lentivirus for 4-6 hours before selection in Dulbecco’s modified Eagle’s medium + 10% fetal bovine serum (Thermo Fisher Scientific) and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: NIH/3T3 or HEK293T cells were plated at 1.5×105 cells per well in 6-well dishes and transfected 24 h after plating using Lipofectamine 2000 (Invitrogen, 11668) and Opti-MEM reduced serum medium (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... After 7 days of differentiation the BMDMs were seeded at a density of 0.8×106 cells/well in 6-well plates in MGM 10/10 media containing Dulbecco’s Modified Eagle Medium (DMEM) + Glutamax (Gibco®) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Neuroscience 2024Quote: ... The dissociated cells were added at 200,000 cells/well to 6-well plates containing astrocyte-coated coverslips in filter-sterilized NBA plus [Neurobasal-A medium (Gibco) supplemented with 1X Glutamax (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... in 6-well tissue culture plates containing 1 ml prewarmed culture medium (42% MEM, 25% Basal Medium Eagle; Thermo Scientific), 25% Normal Horse Serum ...
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bioRxiv - Cell Biology 2024Quote: ... the resuspended cells from above were transferred into a new low adherent 6-well plate (Fisher Scientific. Catalog No.7200601) with ∼ 300k cells per well ...
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bioRxiv - Biophysics 2024Quote: ... This was done by placing 0.2 mg/ml sulfo-succinimidyl-6-(4-azido-2-nitrophenyl-amino) hexanoate (sulfo-SANPAH) (Thermo Fisher) in 0.5 mM pH 8.5 HEPES buffer onto the hydrogel surface and irradiating with ultraviolet (UV ...
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bioRxiv - Cancer Biology 2024Quote: ... 5E6 (5 × 10^6) GSU-Redfluc cells were suspended in 100 uL DPBS (Dulbecco’s Phosphate Buffered Saline; Gibco Inc., USA) and xenografted subcutaneously into NSG mice ...
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bioRxiv - Bioengineering 2024Quote: ... Hepatic endoderm specification was initiated on differentiation days 6 ─ 10 using RMPI supplemented with 2% B27 and insulin (ThermoFisher, #17504044), 10ng/ml FGF-2 ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was carried out in 96-well plates using gene specific primers and a VWR qPCR Master Mix (Avantor) for 40 cycles on a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher).
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bioRxiv - Microbiology 2024Quote: ... 1.0 µg of total RNA was resuspended in 2x formamide loading buffer (Thermo) and run on a precast 6% PAGE gel (Invitrogen) with the Riboruler RNA ladder (Thermo) ...
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bioRxiv - Neuroscience 2024Quote: Total protein from whole cell lysates of mPFC and NAc (6-8 µg) were loaded onto NuPAGE 4-12% Bis-Tris gels (Invitrogen) under reduced and denatured conditions (Pierce 4X LDS sample buffer ...
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bioRxiv - Neuroscience 2024Quote: ... with real time quantitative gene expression determined using using Taqman probles (Table 2) and analysed by the Quant Studio 6 Flex Real-Time PCR System (Invitrogen).
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed 6 times with PBS for 2 min and incubated with Alexa Fluor 568- (1:500 dilution, Invitrogen), Alexa Fluor 488- (1:500 dilution ...
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bioRxiv - Microbiology 2024Quote: ... The human fibrosarcoma cell line HL116 stably expressing the firefly luciferase reporter gene under the control of the immediate early IFN-I inducible 6-16 promoter (kindly provided by Dr. S. Pelligrini, Institut Pasteur, France49) was maintained under HAT selection (Gibco; 21060017 ...