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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Mixed peptides were re-dissolved in 300 µl 0.1% trifluoroacetic acid and fractionated according to the instructions of Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher, Cat# 84868). Samples were eluted by gradient acetonitrile of 10.0% ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). For this ...
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bioRxiv - Cancer Biology 2023Quote: Digested peptides were run on a Q-Exactive HF mass spectrometer coupled to an EASY-nLC II 1200 chromatography system (Thermo Scientific). Samples were loaded on a 50 cm fused silica emitter that was packed in-house with ReproSIL-Pur C18-AQ ...
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bioRxiv - Cell Biology 2023Quote: ... and about 1 µg peptide was loaded onto a 2-cm trap column and separated by a 50-cm EASY-Spray column (Thermo Scientific) heated to 55°C ...
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bioRxiv - Biochemistry 2023Quote: ... Interaction of His-fused proteins with other purified proteins (e.g. GST-PKAC) or synthetic peptides was studied by pulldown using Ni-NTA affinity resin (HisPur, Thermo scientific # 88221). GST-fused proteins were pulled down on glutathione-Sepharose beads (Amersham Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Cancer Biology 2023Quote: Peptides were resuspended in 200 mM HEPES (pH 8.5) and a 100µg aliquot of peptides was mixed with tandem mass tags (TMT, ThermoFisher Pierce, Rockford, IL) at a label to protein ratio of 2:1 ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was then added to a new tube and the peptides were extracted and desalted using C18 desalting columns (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pooled and desalted sample was fractionated using high pH reverse-phase chromatography on an XBridge peptide BEH column (130 Å, 3.5 μm, 2.1 × 150 mm, Waters) on an Ultimate 3000 HPLC system (Thermo Scientific/Dionex). Buffers A (10 mM ammonium formate in water ...
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bioRxiv - Cell Biology 2023Quote: ... samples were loaded offline onto 3-phase chromatography columns and peptides were eluted using 10 MudPIT steps into an Orbitrap Elite mass spectrometer in positive ion mode (Thermo Scientific) using an Infinity 1260 quaternary pump (Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... Trypsinized peptides were then analyzed on an EASY-nLC II system connected to an Orbitrap Elite ETD hybrid mass spectrometer (Thermo Fisher) using Thermo Scientific™ Xcalibur™ Software ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mg of peptides were separated on a reverse phase C18 column and analyzed on QExactive HF Mass Spectrometer (Thermo-Fisher Scientific) as described previously 40 ...
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bioRxiv - Biochemistry 2024Quote: ... The tryptic digest was separated through online reversed-phase chromatography using a peptide trap column Acclaim PepMap 100 C18 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Desalted peptides (100 μg) from each sample were labeled with one of the TMT-10plex reagents (0.8 mg, Thermo Fisher Scientific) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of plasma exosome peptides (pooled from 10 different plasma exosome samples) were injected onto Dionex Ultimate 3000 LC (Thermo Scientific) coupled to ACQUITY UPLC CSH1.7 mm C18 column (2.1 × 150 mm ...
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bioRxiv - Biochemistry 2024Quote: ... was used as a reference protein database and peptide spectra matches were performed using the SEQUEST algorithm within Proteome Discoverer v.2.1 (Thermo Fisher Scientific) with a fragment tolerance of 0.6 Da and parent tolerance of 10 ppm ...
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bioRxiv - Immunology 2024Quote: ... were immunized subcutaneously at two sites on the back flank area with 200 μg of MOG35–55 (MEVGWYRSPFSRVVHLYRNGK) peptide (Thermo Fisher) emulsified in 200 μl incomplete Freund’s adjuvant (BD bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... LC-MS analyses of peptide samples were performed on a hybrid ion trap- Orbitrap mass spectrometer (LTQ-Orbitrap Velos, Thermo Scientific) coupled with nanoflow reversed- phase liquid chromatography (EASY-nLC 1200 ...
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bioRxiv - Microbiology 2024Quote: ... For LC-MS/MS measurements 800 ng of peptides were analyzed on an LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLCTM 1000 UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... peptide samples were then dried in a speedvac and resuspended in a solution of 3% acetonitrile and 0.1% TFA and desalted using Pierce Peptide Desalting Spin Columns (Thermo Scientific # 89851). Eluants were dried in a speedvac and resusupended in a solution of 3% acetonitrile and 0.1% formic acid to a final concentration of 0.5 µg/µL ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
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bioRxiv - Cancer Biology 2024Quote: 50 µg of the of the resulting peptides after tryptic digest as described above were subsequently labeled using TMT-10plex reagents (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (Thermo Scientific), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Cancer Biology 2024Quote: ... About 5 µg digested peptides were aliquoted for global proteomics analysis by stage tip desalting and dried using Speed-Vac (Thermo Scientific). 1 µg peptides were loaded on Evotip (Evosep Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Separated peptides were eluted directly from the column and sprayed into an Orbitrap Velos Mass Spectrometer (ThermoFisher Scientific, Hemel Hempstead, UK) using an electrospray capillary voltage of 2.7 kV ...
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bioRxiv - Cell Biology 2024Quote: LC-MS/SM analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were eluted for 35 minutes onto a 0.075 mm × 500 mm Acclaim™ PepMap™ RSLC resolving column (Thermo Scientific) using a liner gradient ranging from 5-28% of buffer B (80% Acetonitrile/0.1% Formic Acid/19.9% Water ...
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bioRxiv - Neuroscience 2024Quote: ... Oligomers of Aβ42 were prepared by diluting the peptide solution in phenol red-free F12 medium (Thermo Fisher, Waltham MA, USA) to a final concentration of 100 μM and incubated for 24 h at 4°C followed by immediate use or storage at -80°C ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µl of this peptide mix was injected onto an Acclaim PepMap RSLC HPLC column (75 µm × 15 cm, Thermo Scientific) connected to the EASY-nLC 1000 system (Thermo Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... A one microgram equivalent of peptide was subjected to LCMS/MS analysis on Orbitrap Q-Exactive Plus mass spectrometer (Thermo Scientific) in conjunction with Dionex UltiMate3000 Ultra High-Performance Liquid Chromatography (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... and were subjected to separation on an XBridge Peptide BEH C18 column (Waters) connected to an Accela high-performance liquid chromatography (HPLC) system (Thermo Scientific). A 50-min gradient of buffer B (90% ACN ...
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bioRxiv - Biochemistry 2024Quote: ... The peptide samples were loaded onto an Acclaim PepMapTM 100 C18 trap column (100 µm x 2 cm, 100 A; Thermo Scientific) and resolved on a PepMap RSLC C18 analytical column (2µm ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide concentration was calculated based on the peak area of 100 ng of Pierce HeLa protein digestion standard (Thermo Fisher Scientific). Afterwards the peptide solution was frozen at −70°C before further processing.
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bioRxiv - Bioengineering 2024Quote: ... to peptides detected using DECODE was performed as follows: 12.5µL per well of 4 mM peptide solutions were coated on 384 well ELISA plates™ (Thermo Fisher Scientific) by shaking at r.t ...
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bioRxiv - Biochemistry 2024Quote: ... cell lysates were spiked with a known concentration of synthetic stable isotope-labeled peptides (Gerber et al, 2003) (AQUA) obtained from Life Technologies: ATPGHTG[C]LSPG[C]PDQPA(R ...
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bioRxiv - Developmental Biology 2024Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ Quantitative Colorimetric Peptide Assay (23275) and Pierce™ Rapid Gold BCA Protein Assay Kit (A53227) were ordered from ThermoFisher Scientific (Waltham ...
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bioRxiv - Microbiology 2024Quote: ... Eluting peptides were directly analyzed on a Q-Exactive HF mass spectrometer in data-dependent acquisition (DDA) mode (Thermo Fisher Scientific)15 ...
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bioRxiv - Cell Biology 2024Quote: The resulting peptides were analyzed by LC-MS using Nano LC-MS/MS (Dionex Ultimate 3000 RLSCnano System, Thermo Fisher Scientific) interfaced with Eclipse (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... sonicated and the peptide concentration was determined through absorbance at 280 nm measurement using a Nanodrop instrument (ThermoFisher, Nanodrop One C).
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bioRxiv - Microbiology 2024Quote: ... Isobaric-labelled peptides from total proteome and phosphoproteomes were then fractionated into eight fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Peptides were analysed through Ultimate 3000 RSLC nano-UPLC system connected with an Orbitrap Elite hybrid mass spectrometer (Thermo Fisher Scientific). Roughly 600 ng of peptides were loaded with automated injections onto a trap column (Acclaim PepMapTM 100 ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μL of peptide digests were loaded onto a C18 PepMap trap column (300 μm inner diameter x 5 mm, Thermo Fisher Scientific) at a flow rate of 30 μL/min ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulted peptides were separated by a LC gradient of two hours and analyzed on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). The MS data were searched against the mouse database using the MaxQuant software ...
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bioRxiv - Cancer Biology 2021Quote: ... An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18, 5 μm particles, 20 mm length, Thermo Fisher Scientific) at 8 μL/minute ...
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bioRxiv - Cell Biology 2020Quote: ... for 120min at 300nl/min was used to elute peptides from the capillary reverse-phase column (0.075 x 250mm, Pepmap®, Fisher Scientific, 164941). Data were acquired using the Xcalibur software (version 4.0) ...
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bioRxiv - Genetics 2021Quote: The coding sequence for the pH insensitive Citrine fluorescent protein was inserted in the coding sequence of the BK2L3 protein (Zm00001d034049) immediately after the N-terminal secretory peptide domain and synthesized in the pDONOR221 vector (Life Technologies, USA) (Tian et al. ...
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bioRxiv - Genetics 2021Quote: ... peptides were analyzed by liquid chromatography tandem mass spectrometry (LC MS/MS) with an Easy-nLC 1000 (Thermo Fisher, San Jose, CA) coupled to an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... A volume corresponding to 400ng of peptides was used for the analysis using an LC 1200 ultra-high-pressure system (Thermo Fisher Scientific) coupled via a nano-electrospray ion source (Thermo Fisher Scientific ...