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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: 1.5 μg of peptide was injected on an UltiMate 3000 nano LC system online coupled to a Q-Exactive HF-X instrument (Thermo Scientific). Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were ionized by nano-electrospray at 2 kV and analyzed on an Orbitrap Exploris 480TM (Thermo Fisher Scientific, Bremen, Germany) MS ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting peptides were resolved on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 1% acetic acid/acetonitrile gradient at 300 nl/min and directly introduced into Q Exactive HF mass spectrometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: Peptides were constructed on a CEM Liberty 12-Channel Automated Microwave Peptide Synthesizer using Fmoc-PAL-PEG-PSTM (Rink resin; loading capacity 0.21 mmol/g) (ThermoFisher, Cat.#: GEN913383) and Fmoc-protected amino acids (GL Biochem (Shanghai)) ...
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bioRxiv - Neuroscience 2022Quote: ... acetonitrile 0.1% (v/v) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Neuroscience 2022Quote: Chemicals and reagents specifically used for proteomic analysis include: Pierce™ high pH reversed-phase peptide fractionation kit (Cat. No.: 84868; ThermoFisher), High-Select™ Fe-NTA phosphopeptide enrichment kit (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated on an Ultimate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) with a linear gradient reaching 55% MS solvent B after 145 min and 99% MS solvent B at 150 min ...
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bioRxiv - Microbiology 2022Quote: ... 25 μg of peptides from each sample were labeled with 0.25 mg of the TMTpro TMT-labeling reagent (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Peptide LC-MS analysis was performed with a Dionex 3000 nano-HPLC coupled to an Orbitrap XL mass spectrometer (Thermo Fisher). Peptide samples were pressure-loaded onto a home-made C18 reverse-phase column (75 µm diameter ...
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bioRxiv - Cancer Biology 2022Quote: ... was determined by measuring the phosphorylation of a FRET peptide substrate (Ser/Thr19) labeled with Coumarin and Fluorescein using Z’-LYTE™ assay (Invitrogen). The 10 µL assay mixtures contained 50 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2019Quote: ... pull-down and sample preparation was performed as described (Wendrich et al., 2017).c Peptides were applied to online nano LCMS/MS (Thermo Scientific) using a 60 minute acetonitrile gradient from 5-50% ...
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bioRxiv - Molecular Biology 2019Quote: The purified digested peptides were separated with reverse phase liquid chromatography using a Dionex Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) and analyzed by tandem mass spectrometry using Orbitrap Fusion mass spectrometry (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... MpPHOT proteins were detected with rabbit antibody raised against the custom-made synthetic peptide (C+VDERAPPSKGSAKE) (Eurofins Genomics) and horseradish peroxidase-conjugated anti-rabbit IgG (Thermo Scientific). Both antibodies were used at dilution of 1:2000 ...
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bioRxiv - Cell Biology 2019Quote: LC-MS/MS analyses of peptide mixture were done using RSLCnano system connected to Orbitrap Elite hybrid spectrometer (Thermo Fisher Scientific) with ABIRD (Active Background Ion Reduction Device ...
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bioRxiv - Biochemistry 2020Quote: ... Injected peptide samples were on-line de-salted/concentrated using an Acclaim PepMap C18 trap column (75 μm × 2 cm, Thermo Scientific) for 2 min at 5uL/min using 2% MeCN + 0.2% FA ...
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bioRxiv - Biophysics 2019Quote: ... Peptide amides were cleaved from the resin with 95% (v/v) aqueous trifluoroacetic acid solution (TFA, Fisher Scientific, Loughborough, United Kingdom) containing 5% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: Peptide fractions were analyzed by nanoLC-MS/MS on a Thermo Orbitrap Fusion hybrid mass spectrometer (Q-OT-qIT, Thermo Scientific) equipped with an EASY-NLC 1000 system (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... 4 × 105 mouse splenocytes/well were plated with either with 5 μg/ml YFV-17D NS3 ATLTYRML peptide antigen (57) or with 50 μg/ml of total Vero E6 cellular antigen in RPMI 1640 medium (Gibco, Belgium) supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2019Quote: ... The peptide samples were loaded on a nano viper trap column (C18, 5u, 100Å, 100um X 2 cm; PN: 164562, Thermo Scientific) and resolved on a 50 cm analytical column (2 um ...
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bioRxiv - Biochemistry 2019Quote: ... The site of phosphorylation of 32P-labeled peptides was determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer of the peptide coupled to 80 Sequelon-AA membrane (Applied Biosystems).
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bioRxiv - Plant Biology 2019Quote: ... Digested peptides were extracted from the gel by peptide extraction buffer (50% acetonitrile, 0.1% formic acid) and dried in a vacuum concentrator (Thermo Fisher Scientific). Peptides were resuspended in 0.1% formic acid and desalted using C18 ZipTips (Millipore ...
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bioRxiv - Microbiology 2020Quote: Peptide samples were analyzed on an Orbitrap Fusion Tribrid mass spectrometer interfaced with Easy-nLC1200 liquid chromatography system (Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm ...
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bioRxiv - Molecular Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Cell Biology 2019Quote: LC-MS/MS analyses of peptide mixture were done using RSLCnano system connected to Orbitrap Elite hybrid spectrometer (Thermo Fisher Scientific) with ABIRD (Active Background Ion Reduction Device ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were measured on a QExactive HF Mass Spectrometer coupled to a Dionex UltiMate 3000 UHPLC system (both Thermo Fisher Scientific) equipped with an in house-packed C18 column (ReproSil-Pur 120 C18-AQ ...
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bioRxiv - Genomics 2019Quote: ... Peptides in solution were dried by speed vacuum and reconstituted in 50 mM NH4OH and quantified by BCA (Thermo Fisher Scientific). Basic reverse phase fractionation was carried out on 8 μg of tryptic peptides using an XBridge BEH C18 column (130 Å ...
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bioRxiv - Microbiology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min acetonitrile gradient ...
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bioRxiv - Immunology 2021Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37◦C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
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bioRxiv - Immunology 2020Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37°C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Dried peptide mix was resuspended in 2% TFA and fractionated using Pierce High pH Reversed-Phase Fractionmation kit (84868, ThermoFisher Scientific). Each fraction was vacuum-dried and resuspended in 25 μl 0.1% TFA ...
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bioRxiv - Microbiology 2020Quote: Both WCP and vRIC peptides were analysed using a Dionex Ultimate 3000 RSLC nanoUPLC (Thermo Fisher Scientific Inc, Waltham, MA, USA) system online with an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific Inc ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were trapped on a C18 PepMap100 pre-column (300 μm inner diameter × 5 mm, 100 Å pore size, Thermo Fisher) using solvent A [0.1% (v/v ...
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bioRxiv - Pathology 2021Quote: Peptide samples were analysed by nano-LC-MSMS (Dionex RSLCnano 3000) coupled to a Q-Exactive Orbitrap mass spectrometer (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2019Quote: ... Tryptic peptides were separated by nanoscale C18 reverse phase chromatography coupled on line to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) via a nanoelectrospray ion source (Nanospray Flex Ion Source ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were separated on an EASY-Spray HPLC column (25 cm × 75 µm ID, PepMap RSLC C18, 2 µm, Thermo Scientific) using a gradient of 5 - 35% acetonitrile in 0.1% formic acid and a flow rate of 300 nL/min for 45 minutes delivered by an EASY-nLC 1000 nUPLC (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Electrospray MS was performed on the tryptic peptides using an LTQ XL linear quadrapole ion-trap mass spectrometer equipped with in-line liquid chromatography (ThermoFisher Scientific) using a C8 capillary column (300 x150 mm ...
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bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were loaded onto a C18-reversed phase column (75-μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific), separated and MS data acquired using Xcalibur software ...
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bioRxiv - Biochemistry 2019Quote: ... The application of a 2.0 kV distal voltage electrosprayed the eluting peptides directly into the Thermo Fusion Tribrid mass spectrometer equipped with an EASY-Spray source (Thermo Scientific). Mass spectrometer-scanning functions and HPLC gradients were controlled by the Xcalibur data system (Thermo Scientific) ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... the rhodopsin was eluted in 2mL elution buffer containing 1.25 mg (265 μM) Pierce™ 3x DYKDDDDK Peptide (Thermo Scientific, USA). The eluate was concentrated using an Amicon Ultra-2 Centrifugal Filter Unit with Ultracel-10 membrane (Millipore ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Re-dissolved samples were loaded on a ReprosilPur C18 reverse phase column and peptides were analyzed using a nano-HPLC Dionex Ultimate 3000 system (Thermo scientific) coupled to an Orbitrap Velos mass spectrometer (Thermo scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Custom PP2A-Aα antibody was generated by immunizing rabbits with the peptide sequence of MAAADGDDSLY conjugated to matriculture Keyhole Limpet Hemocyanin (Thermo Scientific). Antigens were injected into New Zealand White rabbits using an institutionally approved protocol and animal-care facility (Pocono Rabbit Farm and Laboratory ...
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bioRxiv - Cell Biology 2019Quote: ... antisera were diluted with 0.1 vol 10X PBS and incubated overnight with immunizing peptide covalently coupled to sulfo-link resin (Thermo Fisher Scientific). After washing with PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of the peptide was determined using the BCA method and passed through a vacuum centrifugal concentrator (Thermo Fisher, USA), and the dried peptides are sealed and stored at −80°C ...
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bioRxiv - Neuroscience 2019Quote: ... The reconstituted peptides were separated by two-dimensional nano reverse-phase liquid chromatography (Dionex NCS-3500 UltiMate RSLCnano UPLC) EasySpray NanoSource (Thermo Scientific), ionized using an EasySpray NanoSource (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... About 1.5 micrograms of peptides from each sample were used for DIAMS measurement on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific) platform coupled to a nanoelectrospray ion source ...
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bioRxiv - Systems Biology 2020Quote: ... Single U-937 monocyte and macrophage cells were isolated and distributed into 1μl of pure water with MassPREP peptide mixture (25fmol/μl final concentration, Waters 186002337) in 384-well PCR plates (ThermoFisher AB1384) using a BD FACSAria I cell sorter ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were analyzed by LC-MS/MS using a data-independent acquisition method as described previously.69 Extracted ion chromatograms for fragment ions derived from tryptic peptides were plotted using Xcalibur Qual Browser (Thermo Scientific). The area under the curve for each peak was used for quantification.
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bioRxiv - Physiology 2020Quote: ... 50 μg of peptides from each sample were labelled with 5 μl of Tandem Mass Tag 10-plex (TMT, Thermo Scientific) reagent (0.02mg/μl ...
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bioRxiv - Systems Biology 2020Quote: ... peptides were fractionated at pH 10 with a ‘spider fractionator’ coupled to an EASY-nLC 1000 chromatography system (Thermo Fisher Scientific) as described previously31 ...