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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Each fraction of tryptic peptides was injected and separately analysed on an Ultimate 3000 HPLC system coupled to a QExactive Orbitrap (Thermo Fisher Scientific). The trap (5 mm × 300 µm ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of protein from each condition was subjected to in-solution digestion before labelling the resultant peptides using the TMT-10plex Isobaric Label Reagent Set (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Eluting peptides were ionized by electrospray ionization and analyzed in a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) with the following parameters ...
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bioRxiv - Molecular Biology 2023Quote: Phosphoribosylated peptides were analyzed on the EASY-nLC 1200 coupled to an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher Scientific, San Jose, CA). Peptides were separated on an Aurora UHPLC Column (25 cm × 75 μm ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Peptides in the compiled sample were fractionated (8 fractions) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat: 84868; Thermo Fisher Scientific). Prior to liquid chromatography–mass spectrometry (LC-MS ...
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: LC-MS/MS of pTyr peptides was carried out on an Agilent 1260 LC coupled to a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific). Peptides were separated using a 140-minute gradient with 70% acetonitrile in 0.2 mol/L acetic acid at flow rate of 0.2 mL/minute with approximate split flow of 20 nL/minute ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptide separation for the phosphoproteome analysis was carried out with an Acclaim™ PepMap™ 100 C18 column (Thermo Fisher Scientific) using a 155min non-linear gradient from 3 to 35% of B (0 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were analyzed by online nano liquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus, Thermo Fisher Scientific) using a 120 min gradient ...
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bioRxiv - Systems Biology 2023Quote: ... The acetonitrile was evaporated in a SpeedVac at 45°C and peptide concentration was determined by measuring absorbance at 280 nm on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2023Quote: ... The proteins were trypsinized and the resulting peptides were separated by HPLC and analyzed by LC-S/MS on Q-Exactive (Thermo Fisher Scientific). Data analysis was done as described in (Kurmanbayeva et al ...
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bioRxiv - Plant Biology 2023Quote: A data-dependent LC-MS/MS analysis was performed on the extracted peptide mixtures after digestion using a Fusion tri-hybrid orbitrap mass spectrometer (Thermo Fisher Scientific) interfaced through a nano-electrospray ion source to a RSLC 3000 HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... These peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by Speed Vac (Thermo Fisher Scientific, Bremen, Germany). Then ...
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bioRxiv - Plant Biology 2023Quote: Generated peptides were analyzed on a Dionex Ultimate 3000 RSLCnano system coupled to a Q-Exactive HF-X mass spectrometer (Thermo Fisher Scientific) in data-dependent acquisition mode ...
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bioRxiv - Plant Biology 2024Quote: ... 1 to 3 µl of each sample were subjected to reverse phase LC for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were loaded on a trapping column and eluted over a 75μm x 50cm analytical column (Thermo Fisher P/N ES-803) at 300 nL/min using a 1hr reverse phase gradient ...
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bioRxiv - Microbiology 2024Quote: ... Extracted and desalted [45] peptides were analysed on an Easy-nLC 1200 system coupled to a Q Exactive HF mass spectrometer (all Thermo Fisher Scientific) [44] ...
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bioRxiv - Cancer Biology 2023Quote: ... dried peptides were resuspended in LC buffer and subjected to DDA-MS on a QExactive H-FX mass spectrometer (Thermo Fisher Scientific) equipped with an Easy-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... An equal amount of each peptide sample was loaded into an Easy-nLC 1000 nano-LC system (Thermo Scientific, Waltham, MA, USA) coupled with a quadrupole Orbitrap mass spectrometer (Q ExactiveTM ...
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bioRxiv - Physiology 2024Quote: ... Liquid Chromatography with tandem mass spectrometry analysis of the digested labeled peptides was then carried out employing an EASY-nLC 1000 UPLC system (Thermo Fisher Scientific) incorporating a 50 cm EASY-Spray Column coupled with an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were eluted on an Acclaim PepMap 100 C18 analytical column (75 µm × 50 cm, 3 µm particle size; Thermo Fisher Scientific) at 300 nL/min using the following gradient of solvent B ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic peptides (2 µg) were loaded onto a C18 trap (75 µm × 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 µL/min of solvent A (0.1% formic acid and 3% acetonitrile in LC-mass spectrometry grade water) ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific). For this purpose ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 0.5 µg peptides per sample were automatically loaded to the online coupled ultra-high-performance liquid chromatography (UHPLC) system (Ultimate 3000, Thermo Fisher Scientific). A nano trap column was used (300-µm ID X 5 mm ...
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bioRxiv - Cell Biology 2024Quote: Processed peptides were analysed by nanoflow liquid chromatography mass spectrometry (LC-MS) using an EASY-nLC 1000 Liquid Chromatograph (Thermo Fisher Scientific) connected to a custom-made 30-cm capillary emitter column (75μm inner diameter ...
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bioRxiv - Neuroscience 2024Quote: LC-MS/MS analysis of the resultant peptides was performed on an UHPLC connected to a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific) through a nano electrospray ion source (AMR Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were desalted for 4 minutes with Thermo Scientific POROS R2 reversed-phase resin (Thermo Scientific POROS R2 reversed-phase resin 1112906) hand packed into a trap column (1 mm ID × 2 cm ...
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bioRxiv - Biochemistry 2024Quote: Whole proteome: Eluting peptides were ionized in a nanoESI source and on-line detected on a QExactive HF mass spectrometer (Thermo Fisher Scientific). The mass spectrometer was operated in a TOP10 method in positive ionization mode ...
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bioRxiv - Biochemistry 2024Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto a trap column (PepMap Acclaim C18, 5 mm × 300 μm ID, 5 μm, 100 Å, Thermo Fisher Scientific) with loading buffer (100% water ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto a trap column (PepMap Acclaim C18, 5 mm × 300 μm ID, 5 μm, 100 Å, Thermo Fisher Scientific) with loading buffer (100% water ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto a trap column (PepMap Acclaim C18, 5 mm × 300 μm ID, 5 μm, 100 Å, Thermo Fisher Scientific) with loading buffer (100% water ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto a trap column (PepMap Acclaim C18, 5 mm × 300 μm ID, 5 μm, 100 Å, Thermo Fisher Scientific) with loading buffer (100% water ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: 1 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Systems Biology 2024Quote: ∼800 ng of digested and purified peptide mixture was loaded onto an LC-MS/MS system consisting of an EASY-nLC 1000 nanoflow liquid chromatograph (Thermo Fisher Scientific) coupled with either a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were concentrated using a PepMap100 C18 pre-column (75 µm x 2 cm, with 3 µm particle size; Thermo Fisher Scientific) and subsequently resolved on an EASY-Spray column (ES903 ...
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bioRxiv - Microbiology 2024Quote: ... The peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 180 min gradient for fractions and a 480 min gradient if no fractionation was performed ...
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bioRxiv - Microbiology 2024Quote: The digested peptides were analyzed by nanoflow reverse-phase LC followed by tandem MS using a Q Exactive hybrid mass spectrometer (Thermo Fisher Scientific). The capillary reverse-phase HPLC-MS/MS system was composed of a Dionex U3000 gradient pump equipped with a VICI CHEMINERT valve ...
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bioRxiv - Biochemistry 2024Quote: ... One µg of peptide was loaded onto a PepMap 100 C18 trap column (75 µm id x 2 cm length; Thermo Fisher Scientific) at 3 µL/min for 10 min with 2 % acetonitrile (volume fraction ...
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bioRxiv - Microbiology 2020Quote: ... The digestion was stopped with 4% formic acid (FA) (#94318, Fluka) and digested peptides were purified with C18 Spin Columns Pierce™ (#89870, ThermoFisher Scientific, USA). Peptides were eluted with 2 x 80% Acetonitrile (ACN ...
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bioRxiv - Cell Biology 2020Quote: ... The eluted peptide samples were analyzed by a Q Exactive Plus™ hybrid quadrupole-Orbitrap™ mass spectrometer (Catalog No. IQLAAEGAAPFALGMAZR, ThermoFisher Scientific). The electrospray voltage was set to 2.0 kV ...
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bioRxiv - Cell Biology 2020Quote: Each TMT labeled peptide set was fractionated to improve depth of proteome coverage using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific, Part 84868). The fractionation was performed according to the manufacturer’s protocol and yielded 8 peptide fractions for analysis ...
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bioRxiv - Cell Biology 2020Quote: Nanoflow LC-MS analysis of tryptic peptides was performed using a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific 17) connected to an EASYnLC 1200 ultra-high-pressure system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The samples were subjected to tryptic digestion and the resulting peptide mixtures were analyzed by nano-ESI-LC-MS/MS (Thermo Scientific LTQ Orbitrap). Proteins were identified using Mascot (Matrix Science ...
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bioRxiv - Biochemistry 2020Quote: ... and ~200 ng of peptides were injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting peptides were desalted and analyzed by LC-MS/MS on a Q-Exactive plus mass spectrometer (Thermo-Fisher scientific, Pittsburgh, PA) fitted with a capillary HPLC (easy nLC 1000 ...
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bioRxiv - Biochemistry 2022Quote: ... Labeled peptides were first trapped on an Acclaim™ PepMap™ 100 C18 precolumn (5 μM, 0.3 mm X 5 mm, Thermo Fisher Scientific) and eluted to the analytical column nanoEase M/Z Peptide BEH C18 Column (130Å ...