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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Eluted peptides were introduced directly via a nanospray ion source into a Q Exactive Plus hybrid quardrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). The mass spectrometer was operated in standard data dependent mode ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were separated on an EASY Spray C18 column (50 cm x 75 cm, 2 µm particle size) (ThermoFisher Scientific, Toronto) using an EASY nLC-1200 ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptide mixture concentration was estimated by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo Scientific, San Jose, CA, USA) and a standard curve made from MassPREP E ...
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bioRxiv - Biochemistry 2022Quote: ... The amount of peptide material in each resuspended sample was approximately quantified with a NanoDrop OneC microvolume UV-Vis spectrophotometer (Thermo Fisher Scientific), and typically ∼1 µg of the peptide was injected onto the LC-MS system for each run ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were first trapped on a C18 precolumn (Acclaim PepMap 100, 300 μm × 5 mm, 5 μm, 100 Å) (Thermo Fisher Scientific) and eluted peptides were subsequently separated on a μPAC 50 column (PharmaFluidics ...
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bioRxiv - Cancer Biology 2022Quote: ... acetonitrile 0.1% (v/v) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (ThermoFisher Scientific, Inc) over a 150 min organic gradient ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting peptides were analyzed with a nanoAcquity UPLC system (Waters) coupled to an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific) employing the LC-MS/MS protocol in a data-independent acquisition mode ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were passed through Oasis HLB cartridges (Waters, USA) for desalting and then dried by vacuum evaporation (Thermo Fisher Scientific, Germany).
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bioRxiv - Biochemistry 2022Quote: ... Mixed peptide samples were prepared from 9 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspended peptides were evaluated with the Impact II UHR-TOF MS system (Bruker Daltonics Ltd.) coupled with the nanoLC system (Thermo Fisher Scientific). The protein quantification in individual samples was conducted using Maxquant 1.6.6.0 and the Andromeda search engine to correlate MS/MS spectra to the Uniprot Rattus norvegicus database [21] ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting peptides were analyzed with a nanoAcquity UPLC system (Waters) coupled to an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific) employing the LC-MS/MS protocol in a data-independent acquisition mode ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were on-bead digested and tryptic peptides were analyzed by nanoflow-LC-MS/MS with a high performance nanoflow-HPLC Orbitrap based ms/ms system (Thermo Fisher Scientific). The raw data files were analyzed with MaxQuant software (version 1.5.3.30) ...
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bioRxiv - Immunology 2022Quote: ... followed by in gel tryptic digestion and the resulting peptides in the supernatant were concentrated and analysed by LC-MS/MS on a LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) as described (Morikawa et al. ...
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bioRxiv - Immunology 2022Quote: ... and citrullinated Histone 4 using biotinylated peptides (Cit3-His4 bio: SG[Cit]GKGGKGLGKGGAKRHGSGSK-biotin) on streptavidin-coated high-capacity pre-blocked plates (Thermo Fisher Scientific) and hIgG1 at 5 μg/ml in RIA buffer (1% BSA ...
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bioRxiv - Plant Biology 2022Quote: ... the peptides were analyzed by online nano flow liquid chromatography tandem mass spectrometry performed on an EASY-nanoLC 1200 system (Thermo Fisher Scientific) connected to a Q Exactive™ Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... Digested peptides were analysed by LC-MS/MS with a Dionex Ultimate 3000 RSLC nanoUPLC (Thermo Fisher Scientific Inc, Waltham, MA, USA) system and a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus, Thermo Fisher Scientific) using a 50 min gradient ...
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bioRxiv - Microbiology 2022Quote: ... Extracted proteins were subject to in-solution trypsin digestion and the resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) at the Vienna Research Platform for Metabolomics & Proteomics ...
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bioRxiv - Plant Biology 2022Quote: LC-MS/MS analyses of all peptide mixtures were done using Ultimate 3000 RSLCnano system connected to Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific). Prior to LC separation ...
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bioRxiv - Plant Biology 2022Quote: ... truncated SCPSs versions and cysteine-to-serine replacements (all without signal peptide and Stop codon) were cloned into the secretory expression plasmid pPICZalphaA (Thermo Fisher Scientific). Individual cysteine to serine replacements in SCPSs were amplified from synthetic genes (gBlocks ...
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bioRxiv - Neuroscience 2023Quote: ... C18 column-desalted peptides were resuspended with 100 mM HEPES pH 8.5 and the concentrations were measured by micro BCA kit (Fisher Scientific, Cat # PI23235). For each sample ...
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bioRxiv - Microbiology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 360-min gradient ...
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bioRxiv - Plant Biology 2023Quote: ... constructs coding for mCherry tagged versions of the peptides were generated in pK7m34GW-rolD∷EGFP destination vector using Multisite Gateway Technology (Thermo Fisher Scientific). The entry clones contained the same promoter fragments in pDONRP4-P1R which were used for complementation experiments ...
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bioRxiv - Immunology 2022Quote: We induced EAE using 8-to 12-week-old female mice that were subcutaneously immunization with a 100 μL emulsion of 100 μg of MOG35–55 peptide (MEVGWYRSPFSRVVHLYRNGK) (Thermo Fisher Scientific) in PBS with CFA containing 200 μg of Mycobacterium tuberculosis H37Ra (Difco) ...
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bioRxiv - Neuroscience 2023Quote: ... Then 2-8 µg peptide was dissolved in 20 µL 100 mM TEAB pH 8.5 and 100 µg TMTpro 16plex labels (Thermo Fisher Scientific A44522) dissolved in 5 µL anhydrous acetonitrile was added to each sample ...
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bioRxiv - Physiology 2022Quote: ... Desalted peptides were analysed using liquid chromatography mass spectrometry (LC-MS/MS) using an EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific) coupled to an Q Exactive HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... Sample aliquots containing 25 μg of peptides were dried and labeled with tandem mass isobaric tags (TMT 10-plex, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses of purified and desalted peptides were performed on a Dionex UltiMate 3000 n-RSLC system (Thermo Fisher Scientific), connected to an Orbitrap FusionTM TribridTM mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: Nano-scale liquid chromatography (LC) separation of tryptic peptides was performed on a Dionex Ultimate™ 3000 Rapid Separation LC system (Thermo Fisher). The protein digests were loaded onto a 20 μl nanoViper sample loop (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were separated on an EASY-Spray PepMap RSLC C18 column (2 µm particle size, 75 µm × 50 cm; Thermo Scientific, ES801) heated to 55 °C using a flow rate of 400 nL/min ...
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bioRxiv - Molecular Biology 2022Quote: Label-free peptide samples were subjected to LC-MS/MS analysis on an EASY-nLC 1200 (Thermo Fisher Scientific, San Jose, CA) coupled to a Q Exactive HF Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... synthesized with an added terminal cysteine residue (New England Peptide) was dissolved in ultrapure water with 5 mM EDTA and mixed with maleimide-activated OVA (Thermo Fisher Scientific) at room temperature for 2 h.
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bioRxiv - Evolutionary Biology 2022Quote: ... 2 μl of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Therefore ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of tryptic peptides were used for High pH RP-HPLC using a 25 cm ProSwift™ RP-4H capillary monolithic column (Thermo Scientific) on an Agilent 1200 series HPLC (high-pressure liquid chromatography ...
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bioRxiv - Cell Biology 2023Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 120-min gradient ...
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bioRxiv - Plant Biology 2024Quote: ... 1 to 3 µl of each sample were subjected to reverse phase LC for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm ...
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bioRxiv - Developmental Biology 2024Quote: ... Liquid chromatography-mass spectrometry (LC-MS) analyses of trypsin-digested peptides were performed on a Q Exactive HF-X hybrid Quadrupole-Orbitrap instrument (Thermo Fisher Scientific) coupled with an Easy-nLC 1200 system ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μg of each pooled sample was digested in-solution as described above and fractionated by Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to manufacturer recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were loaded on a trapping column and eluted over a 75μm x 50cm analytical column (Thermo Fisher P/N ES-803) at 300 nL/min using a 1hr reverse phase gradient ...
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bioRxiv - Microbiology 2024Quote: ... Extracted and desalted [45] peptides were analysed on an Easy-nLC 1200 system coupled to a Q Exactive HF mass spectrometer (all Thermo Fisher Scientific) [44] ...
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bioRxiv - Physiology 2024Quote: Peptides for total proteomic analysis were analyzed on a Dionex 3500 nanoHPLC coupled to an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific) via electrospray ionisation in positive mode with 1.9 kV at 275 °C and RF set to 30% ...
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bioRxiv - Developmental Biology 2024Quote: ... Aliquots containing 10 μg of peptide from each sample were labeled with tandem mass tag (TMT) 16-plex reagents (TMTpro, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Baker ™) and peptide concentrations were determined from their absorbance at 280 nm using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, USA).
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bioRxiv - Molecular Biology 2024Quote: 1.5 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were used for conjugation of fluorophore to the puromycylated peptides using the Click-iT ® Plus Alexa 647 Fluor ® Picolyl Azide tool kit (Invitrogen). The pellet of gonads was resuspended and incubated with 200 µL Click-iT ® reaction buffer containing 0.5 µL of 500 µM Alexa Fluor ® Picolyl Azide ...
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bioRxiv - Cell Biology 2024Quote: ... and peptides were analysed via a nanospray ion source into a Q Exactive Plus hybrid quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Peptides were analyzed by nano-HPLC-tandem mass spectrometry (MS/MS) using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific, Dreieich, Germany) connected to an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific ...