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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... An aliquot of each peptide preparation was injected onto a PepMap RSLC C18 analytical column (2 µm, 100 Å, 75 µm x 25 cm, Thermo Scientific) and subject to a 90 min ...
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bioRxiv - Developmental Biology 2020Quote: ... peptides were first loaded onto a trapping cartridge (Acclaim PepMap300 C18, 5 μm particle size, 300 Å pore size, Thermo Fisher Scientific) and washed for 3 min with 0.1 % TFA ...
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bioRxiv - Developmental Biology 2020Quote: ... Peptides were ionized by the application of a 2.5 kV positive voltage applied distally to the peptides eluting into a Q-Exactive Plus (QE+) mass spectrometer (Thermo Scientific, Waltham, MA). Full MS spectra were recorded on the eluting peptides over a 350 to 1700 m/z range at 70,000 resolving power ...
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bioRxiv - Biochemistry 2020Quote: ... and washed for 3 min at 30 μL/min before peptides were eluted onto a C18 AcclaimTM PepMapTM column (100 Å, 5 μm × 0.75 mm × 150 mm, Thermo Fisher Scientific) at flow rate of 0.3 μL/min ...
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bioRxiv - Biochemistry 2022Quote: ... approximately 0.15 μg of peptides were run by online reversed phase chromatography on an Agilent 1290 UHPLC or Dionex UltiMate 3000 (Thermo Fisher Scientific) coupled to a Thermo Scientific Orbitrap Fusion mass spectrometer ...
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bioRxiv - Molecular Biology 2021Quote: ... peptides were analyzed in triplicate using an Ultimate 3000 nano coupled in line with an Orbitrap ELITE (Thermo Scientific, San Jose California). Briefly ...
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bioRxiv - Immunology 2021Quote: ... The peptides from the Hemocyte samples collected at four different time points were labelled with four 8-plex iTRAQ reagents (Applied Biosystems, USA).
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bioRxiv - Systems Biology 2020Quote: ... Up to 1mg peptide was fractionated by reverse-phase high pressure liquid chromatography (HPLC) on an Dionex UltiMate 3000 HPLC system (Thermo Scientific, USA) equipped with an XBridge® BEH C18 Sentry Guard Cartridge pre-column (130Å ...
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bioRxiv - Cell Biology 2019Quote: LC/MS data of the trypsin-Partially-digested peptides were collected on an Orbitrap Fusion mass spectrometer (Thermo Scientific, San Jose, CA) with a Nanoacquity M Class UPLC (Waters Corporation ...
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bioRxiv - Microbiology 2019Quote: ... The peptides obtained after trypsin digestion were analyzed by Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) analyzer (Applied Biosystems, USA). The tandem mass spectrometry (TOF/TOF ...
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bioRxiv - Cell Biology 2019Quote: Liquid chromatography mass spectrometry analysis (LC-MS / MS) of peptide extracts was done using 3000 Nano LC nanochromatographic system (Thermo Scientific, USA) and Maxis Impact mass spectrometer with an electrospray ionization source Captive Spray (Bruker ...
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bioRxiv - Cell Biology 2020Quote: ... The dried peptides were dissolved with 100 mM triethylammonium bicarbonate and then labeled with 6-plex Tandem Mass Tags (TMT) reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... at 37°C with 5% CO2 and transfected by different expression constructs for human IgG-Fc with various signal peptide coding regions using LipofectAmine (Invitrogen, Calsbad, CA) as the transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Eluted peptides were introduced directly via a nanospray ion source into a Q Exactive Plus hybrid quardrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). The mass spectrometer was operated in standard data dependent mode ...
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bioRxiv - Molecular Biology 2019Quote: ... Peptides were separated on reversed phase C18 columns (precolumn: Acclaim PepMap 100, 300 μm × 5 mm, 5μm, 100 Å (Thermo Fisher Scientific); separation column ...
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bioRxiv - Biochemistry 2019Quote: ... The peptides were loaded on a reversed-phase trap column (75 μm × 2 cm, 3 μm, C18, 100 Å, Thermo Scientific, Germany), and then the eluent was transferred to a reversed-phase analytical column (50 μm × 150 mm ...
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bioRxiv - Immunology 2021Quote: TMT-labelled tryptic peptides were subjected to pH reversed-phase (HpRP) fractionation using an Ultimate 3000 RSLC UHPLC system (Thermo Fisher Scientific) equipped with a 2.1 mm internal diameter (ID ...
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bioRxiv - Immunology 2021Quote: ... peptide separation using acetonitrile gradient was run for 120 min and (ii) data was collected on an Orbitrap Fusion (Thermo Fisher Scientific) operated at 120,000 resolution using HCD (higher-energy collisional dissociation ...
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bioRxiv - Neuroscience 2020Quote: ... At this stage the samples included in each 10plex were merged and were fractionated by basic Reverse Phase (bRP) using Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific), generating eight fractions for each of the two 10plexes.
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bioRxiv - Biochemistry 2020Quote: ... Peptides were loaded into a PepMap C18 (5 mm × 300 μm ID, 5 μm particles, 100 Å pore size; Thermo Fisher Scientific) trap column on an UltiMate 3000 RSLC nano HPLC system (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were digested with the filter-aided sample preparation (FASP) method23 and peptides were labeled using TMT 11-plex isobaric reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Peptides were analyzed using a Dionex Ultimate 3000 UHPLC coupled to an Exploris 480 tandem mass spectrometer (Thermo Scientific, San Jose, CA) with sample order randomized ...
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bioRxiv - Biochemistry 2021Quote: ... The FL-NP construct included a C-tag peptide (EPEA) at the C-terminus (27) for affinity chromatography purification using a C-tag affinity resin (Thermo Fisher Scientific), eluting with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... Separation of peptides was achieved by use of an Acclaim Pepmap RSLC analytical column (C18, 100 Å, 75 μm. × 50 cm (Thermo Scientific)) ...
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bioRxiv - Neuroscience 2021Quote: ... The eluted peptides were ionized online by electrospray ionization (ESI) and transferred into an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific) which was operated in the positive mode to measure full scan MS spectra (300–1700 m/z in the Orbitrap analyzer at resolution R = 30,000 ...
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bioRxiv - Neuroscience 2021Quote: ... the peptide extracts were reduced in volume by vacuum centrifugation and a small portion of the extract was used for fluorometric peptide quantification (Thermo scientific Pierce). Samples were analyzed for LC-MS/MS analysis by UC Davis Proteomics core ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were trapped onto a C18 PepMap 100 pre-column (inner diameter 300 mm × 5 mm, 100 Ǻ; Thermo Fisher Scientific) using solvent A (0.1% formic acid in water ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were separated by nano-flow reversed-phase liquid chromatography coupled to a Q Exactive Hybrid orbitrap mass spectrometer (Thermo Fisher Scientific). The peptides were trapped onto a C18 PepMap 100 pre-column (inner diameter 300 mm × 5 mm ...
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bioRxiv - Neuroscience 2019Quote: Each tryptic peptide mixture obtained from 150 μg of protein extract was isotopically labeled with the corresponding TMT6plex reagent (Thermo Fisher Sci.) based on the standard procedure ...
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bioRxiv - Cell Biology 2021Quote: ... The peptide extracts are reduced in volume by vacuum centrifugation and a small portion of the extract is used for fluorometric peptide quantification (Thermo scientific Pierce). One microgram of sample based on the fluorometric peptide assay was loaded for each LC-MS analysis.
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bioRxiv - Plant Biology 2021Quote: The digested peptide mixtures were injected into a liquid chromatography-tandem MS system using an Ultimate 3000 RSLCnano LC (Thermo Fisher Scientific) in-line connected to a Q Exactive (SAMBA callus versus GIF control group and SAMBA leaf ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm ...
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bioRxiv - Microbiology 2020Quote: ... The eluted peptides were sprayed into a Q Exactive hybrid quadrupole-Orbitrap mass spectrometer using a Nanospray Flex™ Ion Sources (Thermo Scientific). Survey scans were taken in the Orbi trap (35,000 resolution determined at m/z 200 ...
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bioRxiv - Systems Biology 2021Quote: ... Peptide sample was loaded onto the pre-analytical column (100 µm x 2 cm, nanoViper C18, 5 µm, 100A; Thermo Fisher Scientific) at a flow rate of 5 µl/min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Tryptic peptides were analyzed using an Easy-nLC1000 liquid chromatography system coupled to a high-resolution Q-Exactive mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... Samples were sequenced by Edman Degradation using a Perkin Elmer 494 Procise Protein/Peptide Sequencer with an on-line 140C PTH Amino Acid Analyzer (Applied Biosystems, Inc).
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded onto an Acclaim PepMap100 C18 trap column (300 µm ID × 5 mm, 5 µm, Thermo Fisher Scientific; Cat. # 160454) and then separated on a PepMap RSLC C18 analytical column (75 µm ID×15 cm ...
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bioRxiv - Systems Biology 2021Quote: ... coupled with a customized LC system as previously described.15 The peptide fractions from high pH reversed-phase LC fractionation were analyzed an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) coupled with a similar customized LC system ...
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bioRxiv - Microbiology 2021Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Neuroscience 2020Quote: High pH reversed-phase peptide fractionation kit was used to perform desalting and fractionation as per manufacturer’s protocol (ThermoFisher, Cat. No. 84868). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Eluted peptides were ionized with a μPAC Flex iON connect (PharmaFluidics, Ghent, Belgium) attached to a Nanospray Flex Ion Source (Thermo Fisher Scientific) equipped with a nanoESI emitter (FOSSILIONTECH ...
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bioRxiv - Plant Biology 2021Quote: ... The OmAGAL2 coding sequence without the expected signal peptide (SP) sequence (ΔSP-OmAGAL, amino acids [AA] 47–412) was cloned into the pENTR/D-TOPO vector (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Six μl of resuspended peptides for the glycoform identification and two μl two-fold diluted peptides for the glycoform quantification were run on an Orbitrap Fusion Tribrid (ThermoFisher Scientific, Bremen) mass spectrometer coupled to nanospray UHPLC system Agilent 1290 (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The resulted peptides were cleaned up in a HyperSep filter plate with bed volume of 40 μL (Thermo Fisher Scientific, Rockford, IL). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were analyzed using a nanoLC-MS/MS system connected to a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific, Germany) equipped with a nano-electrospray ion source ...
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bioRxiv - Cell Biology 2021Quote: 400 ng tryptic peptides per sample were analyzed using an Ultimate 3000 RSLCnano HPLC (Dionex) coupled to a Q Exactive HF Orbitrap (Thermo Fisher Scientific). Samples were pre-concentrated on a C18 trap column (Acclaim PepMap 100 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resultant peptides were analysed on an Advance UHPLC system (AMR/Michrom Bioscience) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific), and the raw mass spectrum was processed using Xcalibur (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus, Thermo Fisher Scientific) using a 140-min gradient ...
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bioRxiv - Biochemistry 2022Quote: ... Tryptic peptides were analysed by nano-scale capillary LC-MS/MS with an Ultimate U3000 HPLC (ThermoFisher Scientific Dionex, San Jose, USA) set to a flowrate of 300 nL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... The peptides were separated on a 75 μm × 15 cm RP-HPLC analytical column packed with 2 μM C18 particles (Thermo Fisher Scientific) using an increasing gradient of 0.1% FA in ACN (8-35% over 100 min ...