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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptides were re-dissolved with 0.1% FA solution for LC-MS analysis (Q-Exactive, Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Plant Biology 2022Quote: ... Five microliters of peptide mixtures were analyzed in duplicate on a Q-Exactive Plus hybrid quadripole-orbitrap mass spectrometer (Thermo Fisher) as described in Pérez-Pérez et al ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated on an Ultimate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) with a linear gradient reaching 55% MS solvent B after 85 min ...
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bioRxiv - Molecular Biology 2022Quote: ... TMT labeled peptides were analyzed by nano-ultrahigh performance (UHP)LC–MS/MS (Easy-nLC1200, Orbitrap Fusion LumosTribrid, Thermo Fisher Scientific). Proteome Discoverer 2.2 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, ThermoFisher, US) using a 180-min gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... that were spiked with 18 pmol shTMTpro-labeled AS192 triggers were fractionated using the Pierce high pH reversed phase peptide fractionation kit (Thermo Scientific). A total of eight fractions were generated ...
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bioRxiv - Plant Biology 2024Quote: ... Desalted peptides (100 μg) from each sample were labeled with one of the TMT-10plex reagents (0.8 mg, Thermo Fisher Scientific) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The two supernatants were combined and subjected to high pH fractionation using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Peptides (100 ng) were separated using Acclaim PepMap C18 columns (2 µm, 100 Å, 75 mm, 15 cm; Thermo Fisher Scientific). The peptides were loaded in buffer A (5% acetonitrile and 0.1% formic acid ...
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bioRxiv - Microbiology 2024Quote: A volume of 6 µl phosphopeptides or 500 ng of peptides were directly loaded onto reversed-phase pre-column (Acclaim PepMap 100, Thermo Scientific) and eluted in backflush mode ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated using an Acclaim PepMap RSLC analytical column (C18, 100 Å, 75 μm × 50 cm, Thermo Fisher Scientific, USA) and Acclaim PepMap trap column (75 μm × 2 cm ...
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bioRxiv - Molecular Biology 2024Quote: Fractionated peptides were resuspended in 0.05% formic acid and separated using an UltiMate 3000 RSLC nano-LC system (Thermo Fisher Scientific) equipped with an analytical column (Waters nanoEase HSS C18 T3 ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Equal amounts of peptide fragments from each experimental group were then labelled utilizing the TMTpro 16plex Isobaric Label Reagent (A44520, Thermo Fisher) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated on a capillary column (0.075 mm × 500 mm, Acclaim Pepmap 100, reverse phase C18, NanoViper, Thermo Fisher Scientific) following a gradient of 2-40% buffer B in 128 min (A = 0.1 % formic acid B = 0.1% formic acid ...
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bioRxiv - Molecular Biology 2024Quote: Peptide samples were analysed in a Data-Independent Acquisition mode (DIA) with an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray ion source and a nano-flow LC system (Easy-nLC 1200 ...
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bioRxiv - Neuroscience 2024Quote: ... the separated peptide was analyzed using DIA (data independent acquisition) mass spectrometry with a Q-Exactive HF-X mass spectrometer (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: The peptides extracted from the SCO or control samples were analyzed using a nanoLC system (Dionex UltiMate 3000, Thermo Fisher Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: LC-MS/MS analysis of peptide samples was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA) coupled to an EASY-nLC 1200 liquid chromatography (LC ...
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bioRxiv - Cell Biology 2024Quote: ... the digest peptides from DMSO and Chk2i conditions were respectively spiked with the synthetic lysine-labelled heavy phospho-peptide of (phos)TLC(Carbamidomethylation)GTPNYIAPEVLSK(+8Da) that was synthesized by Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mg of peptides were separated on a reverse phase C18 column and analyzed on QExactive HF Mass Spectrometer (Thermo-Fisher Scientific) as described previously 40 ...
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bioRxiv - Cell Biology 2023Quote: The processing of BioID raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer v2.3 (Thermo Fisher Scientific). Raw mass spectra were filtered ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Cell Biology 2023Quote: The processing of whole-cell protein quantitation raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer (PD v3.0 (Thermo Fisher Scientific) using a SPS MS3 reporter ion-based quantification workflow ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 300 µL of 0.1% trifluoroacetic acid and desalted using Pierce™ Peptide Desalting Spin Columns (ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched to 0.5% trifluoracetic acid and spiked with Pierce Retention Time Calibrant (PRTC) peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/µL ...
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bioRxiv - Developmental Biology 2024Quote: ... Peptides were extracted from brains from 5 d old male flies and analyzed on an Orbitrap Fusion mass spectrometer (Thermo Scientific) equipped with a PicoView ion source (New Objective ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatography of peptides prior to mass spectral analysis was accomplished using capillary emitter column (PepMap® C18, 3µM, 100Å, 150x0.075mm, Thermo Fisher Scientific). Protein annotations ...
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bioRxiv - Cell Biology 2024Quote: ... Online detection of peptide ions was by electrospray ionisation (ESI) mass spectrometry MS/MS with an Orbitrap Elite MS (Thermo Scientific). Ionisation of LC eluent was performed by interfacing the Sharp Singularity emitters (Fossil Ion Tech ...
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bioRxiv - Cell Biology 2024Quote: ... (Roberta Noberini et al., 2021) Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientific), 25-cm long (inner diameter 75 µm ...
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bioRxiv - Immunology 2024Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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bioRxiv - Microbiology 2024Quote: The synthetic peptides YNGVFQECCQAEDKGACLLPK (STD) and YNGVFQECCQA EDKacGACLLPK (STD*Kac) were analyzed using an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) equipped with an EASY-nLC 1200 system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: Fractionated crosslinked peptides were loaded onto a Vanquish Neo HPLC coupled to a nano-ESI source of an Orbitrap Eclipse (ThermoFisher Scientific). Samples were injected onto a 300μm x 5mm PepMap Neo Trap Cartridge peptide trap column (C18 ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto an Acclaim Pepmap C18 5 µm-particle-size trap column (0.1 × 20 mm, Thermo Scientific #164564-CMD) and separated over a nanoEase M/Z Peptide BEH C18 1.7 µm-particle-size analytical column (0.075 × 250 mm ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto an Acclaim Pepmap C18 5 µm-particle-size trap column (0.1 × 20 mm, Thermo Scientific #164564-CMD) and separated over a nanoEase M/Z Peptide BEH C18 1.7 µm-particle-size analytical column (0.075 × 250 mm ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were collected by centrifugation and labelled using tandem mass tag (TMT) 11-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Elution was performed in 30μl of wash buffer plus 5μl of 1mg/ml 3xFLAG peptide solution (Pierce™ 3x DYKDDDDK, Thermo Scientific, A36805). Samples were incubated on ice for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: 50 µg of the peptides resulting after tryptic digest as described above were subsequently labeled using TMT-10plex reagents (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The tryptic digest was separated through online reversed-phase chromatography using a peptide trap column Acclaim PepMap 100 C18 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Freshly isolated mouse splenocytes or LN cells were stimulated with an overlapping peptide pool of RBDWT (10 μg/mL) in the presence of Brefeldin A (Invitrogen, USA) for 5 h at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: Peptide digests of purified RSVF and antibodies were analyzed in DIA mode on a Thermo Scientific Lumos mass spectrometer (Thermo Fisher) equipped with a nanoelectrospray ion source and coupled to an Easy-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... internal diameter 100 μm) for desalting and a C18 analytical column (length 10 cm, internal diameter 75 μm) for peptide separation (Thermo Scientific). Peptides were eluted with a three-step 45 min gradient of 0.1% formic acid in ACN at a flow rate of 300 nl/min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2023Quote: ... The digested peptides were cleaned-up using the HiPPR detergent removal resin kit (PN 88305, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Fisher Scientific) fitted with a 100 μm × 2 cm PepMap100 C18 nano trap column and a 75 μm × 25cm ...
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bioRxiv - Cell Biology 2023Quote: The TMT-labelled peptide samples were analysed with an Easy-nLC1200 liquid chromatography system combined with Q Exactive HF mass spectrometer (Thermo Scientific) using a 136 min gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately one µg or less for each SEC fraction was injected and the peptides were separated on an Acclaim PepMap RSLC column (150 mm × 75 µm, 2 µm particle size, ThermoFisher Scientific) at a flow rate of 300 nl/min ...
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bioRxiv - Microbiology 2023Quote: ... Multiplexed TMT samples were separated into four fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). TMT labelled fractions were analysed in an Orbitrap Fusion Lumos using a resolution of 50,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides in each sample were labeled with one of the tags of TMTpro 18-plex labeling kit (A52045, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Both the digested peptides from non-enriched and enriched samples were labeled with 16-plex TMT according to the manufacturer’s instructions (Thermo Fisher Scientific). Briefly ...