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Citations for Thermo Fisher :
3201 - 3250 of 3282 citations for GPX6 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Cells were incubated in complete Williams’ E medium and transfected with siRNA (50 nM; Dharmacon) and Lipofectamine® RNAiMAX reagent (ThermoFisher Scientific #13778075) in a final concentration of 1 mL/well Opti-MEM™ ...
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bioRxiv - Molecular Biology 2021Quote: NT2/D1 or HEK293T cells were transfected with the equimolar of DNA constructs using Lipofectamine 2000 and siRNA using Lipofectamine RNAiMax (Invitrogen, Carlsbad, California, USA) as per manufacturer’s guidelines ...
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bioRxiv - Biophysics 2020Quote: ... the ears of the mouse were transfected with K1 siRNA using Lipofectamine 3000 following the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). The sequences of the mouse K1 siRNA were CUCCCAUUUGGUUUGUAGCTT and UGACUGGUCACUCUUCAGCTT (GenePharma ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 500,00 cells were seeded into a 6-well format and were reverse transfected with 20 nM of gene-specific siRNA oligos using RNAiMax (Thermo Fisher Scientific #13778075) and then transfected again after 24 hours using forward transfection with 20 nM of siRNA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with either targeting or non-targeting siRNA constructs which were diluted in OPTI-MEM medium (Life Technologies, Waltham, MA, US) to a final concentration of 20 nM for HAUS6 and HAUS8 in medium with cells ...
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bioRxiv - Cell Biology 2022Quote: ... End1s were transfected with either an ON-TARGETplus non-targeting siRNA pool (Horizon Discovery D-001810-10-05) or an ON-TARGETplus YAP1-targeting siRNA SMARTpool (Horizon Discovery L-012200-00-0005) using Lipofectamine 3000 (Thermo Fisher Scientific L3000008), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: MDA-MB-231 cells were seeded onto 24-well plates and transfected with 15nM of DRP1 siRNA (Thermo Fisher Scientific, Silencer Select, #4390771) or negative siRNA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: GFP-LC3-RFP-LC3ΔG expressing HeLa cells transfected with uchl5 or usp14 siRNA or treated with inhibitors were grown in Thermos ScientificTM NuncTM Lab-TekTM II chambered coverglass plates (Fisher scientific, cat# 16260671) and imaged after 48 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... The following siRNAs were used: control siRNA that have no homology to any sequence in the human genome were used as the controls (Thermo Fisher Scientific, #4390846); PFKFB3 siRNAs (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of the LNA ASOs/siRNA was done using Neon™ Transfection System 100 µL Kit (catalog no. MPK10096, Thermo Fisher Scientific USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfections were done with 150 pmol of siRNA in complete DMEM medium for 8-10 hours using Lipofectamine™ RNAi max (ThermoFisher Scientific, 13778150). Media was changed following transfection incubation ...
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bioRxiv - Cell Biology 2024Quote: ... RTRAF siRNAs were purchased from RiboBio (Guangzhou, China) and transfected into INS-1 cells using Lipofectamine RNAiMAX Reagent (Thermo Fisher Scientific, no. 13778150). Unless otherwise noted ...
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bioRxiv - Cell Biology 2024Quote: ... human ACTA2 (sc-43590, Santacruz, Dallas, Texas) and control siRNA (sc-37007, Santacruz, Dallas, Texas) were transfected into cells with Lipofectamine™ 3000 (Invitrogen, CA, USA) as per manufacturer protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Cell Biology 2024Quote: ... 70% confluent EnSC monolayers in 6-well plates were downregulated overnight in 2% DCC-FBS/DMEM/F12 media and transfected with 50nM Stealth siRNA against human GIPC1 or APPL1 (Fisher Scientific, Loughborough, U.K.). For depletion of receptor expression ...
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bioRxiv - Immunology 2024Quote: ... IDO1 (#MSS209789), IDO2 (#MSS205346), PU.1 (#MSS247676), and IRF4 (#MSS205501), and control siRNA (#12935-300, 12935-200) were purchased from Invitrogen (Carlsbad, CA, USA) and introduced into BMDCs by the electroporation system ...
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bioRxiv - Cell Biology 2024Quote: We transiently transfected NIH3T3 cells with Mecp2 or Nedd4 siRNA (Genema, Shanghai, China) using Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA, #L3000-015) in Opti-MEM medium ...
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bioRxiv - Cell Biology 2023Quote: h-Tert-RPE1 and h-Tert-BJ cells were transfected for 24h (siTPX2 and siCdk5Rap2) 48h (sipericentrin) and 72h (siKif2a) with 20 nM siRNAs using Opti-MEM and Lipofectamine RNAiMAX (Thermofisher; 51985091 and 13778030) in MEM medium (Thermofisher ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were transfected with 15nM siRNA against KLF5 (ONTARGETplus SMART-POOL®, Dharmacon), or with a control siRNA at the respective concentration (ONTARGETplus SMART-POOL®, Dharmacon) using Lipofectamine® RNAiMAX (Invitrogen) following the manufacturer‘s protocol ...
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bioRxiv - Cell Biology 2023Quote: End1s were transfected with either an ON-TARGETplus non-targeting siRNA pool (Horizon Discovery D-001810-10-05) or an ON-TARGETplus YAP1-targeting siRNA SMARTpool (Horizon Discovery L-012200-00-0005) using Lipofectamine 3000 (Thermo Fisher Scientific L3000008), per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 2 × 105 HCT116 cells were reverse-tranfected with 20nM of siRNA siCTRL or siSFPQ-3’UTR(Horizon discovery) in 12-well plates using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, #11668019) diluted in Opti-MEM transfection medium according to the manufacturer’s instructions and incubated for 24 hours at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Cells were transfected with siRNAs against CASC3 or DROSHA (ONTARGETplus SMART-POOL®, Dharmacon), or with a control siRNA (ONTARGETplus SMART-POOL®, Dharmacon) using Lipofectamine® RNAiMAX (Invitrogen) following manufacturers’ protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... For RNAi YAP and TAZ knock down – hMSCs were starved for 6h in DMEM without antibiotics before adding siRNA for YAP or TAZ and scramble control (Stealth RNAi Invitrogen Thermo Fisher Scientific) in OptiMEM with addition of Lipofectamine 3000 (both Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: iSLK-RGB-BAC16 cells seeded in 6-well plates at 50% confluency for 24 h were transfected with siRNAs with lipofectamine RNAiMAX (Thermo Fisher Scientific, 13778150). The knockdown efficiency was confirmed by reverse transcription real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2023Quote: ... cruzi-infected SH-SY5Y cells were transfected again with Lipofectamine RNAiMAX:siST8Sia2 Mix (0.6 µL Lipofectamine:100 nM siRNAs) in Opti-MEM (Thermo Fisher Scientific, Waltham, MA, USA) antibiotic-free medium for an additional 24 h ...
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bioRxiv - Cell Biology 2023Quote: HUVECs on 6-well plates were starved for 24 hours or for siRNA transfection for 48 hours and dissociated by TrypLE Express (Gibco, Grand Island, NY) to obtain cell suspensions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the respective high-complexity siRNA pool (siTOOLs Biotech, Germany) to a final concentration of 3 nM using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with either targeting or non-targeting siRNA constructs which were diluted in OPTI-MEM medium (Life Technologies, Waltham, MA, USA) to a final concentration of 100 nM in the medium with cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty-four hours after plating cells were transfected with two different KCNH2-siRNAs (44858 anti-hERG1 siRNA1 and 44762 anti-hERG1 siRNA2; Ambion; Austin, TX, USA); β1 integrin siRNA (109878 ITGB1 ...
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bioRxiv - Genomics 2024Quote: ... and 50 nM ON-TARGET plus Non-targeting Control siRNAs (Dharmacon, D-001810-04-05) diluted in Opti-MEM™ I Reduced Serum Medium (ThermoFisher Scientific, 11058021) and transfected with Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with non-targeting siRNA (Dharmacon, cat# D-001810-10-05) or siRNA targeting Cdkn1a (Dharmacon, cat# L-058636-00-0005) using Lipofectamine RNAiMAX (Thermo Scientific, cat# 13778-075) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: We transfected 200,000 K562 CRISPRi cells (from the same population of cells that was used in the CRISPRi-FlowFISH screens) with siRNAs (from Ambion, Thermo Fisher Scientific, Table S4) using the Amaxa Nucleofector 96-well Shuttle (Lonza ...
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bioRxiv - Neuroscience 2020Quote: ... because we were not able to identify a specific shRNA target sequence using web-based design tools (InvivoGen siRNA Wizard, and Invitrogen Block-iT RNAi Designer). For calcium imaging ...
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bioRxiv - Molecular Biology 2022Quote: INS-1E or INS-1 832/13 cells were seeded in a 12-well plate and transfected with siRNA using RNAiMAX (Thermo Fisher Scientific, Cat# 13778150). ON-TARGET plus SMARTpool siRNA was used for knocking down rat Wfs1 (Dharmacon ...
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bioRxiv - Cell Biology 2022Quote: ... In mock experiments cells were transfected with equal amount of ON-TARGETplus Control Pool Non-Targeting pool (D-001810-10-05, Dharmacon, CO, USA) or Silencer Select Negative Control #1 siRNA (4390843, Ambion, Thermo Fisher Scientific, MA, USA). All plasmid transfections were performed using Nucleofactor Kit R with the Nucleofactor 2b Device (Lonza ...
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bioRxiv - Molecular Biology 2020Quote: ... or the non-targeting control siRNA pool (Horizon, #D-001810-10-05) 24 hours after seeding the cells using DharmaFECT 2 (Thermo Scientific, #T-2002-03) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... were purchased from Dharmacon (Thermo Scientific, Hemel Hempstead, UK. Cells were transfected with 25 nmol/L siRNA using Lipofectamine RNAiMax transfection reagent (Invitrogen, Life Technologies, Paisley, UK), following the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells were seeded in 6-well plates (5×105/well) were transfected with the indicated siRNA (3 μg) using Lipofectamine® 2000 transfection reagent (Life Technologies, 11668-019) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... HUVEC at passage 3 were transfected twice at 24 h-intervals with 30 nM siRNA and Lipofectamine RNAi max (Life Technologies, ref. 13778-150) according to the manufacturer’s instructions in 37°C in a regular 5% CO2 in a humidified chamber ...
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bioRxiv - Cell Biology 2020Quote: ... The siRNA-resistant form of RAB10 was generated by introducing 3 silent mutations into the target sequence of the seed region of the RAB10-siRNA (Ambion, Thermo Fisher, ID s21391), altering the original sequence by site-directed mutagenesis (QuickChange ...
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bioRxiv - Immunology 2020Quote: ... 1 × 105 cells were transfected with the appropriate plasmid combinations or plasmid/siRNA combinations using the Neon Transfection System (Thermo Fisher, Waltham, MA, USA) and a 10 µL Neon tip at 1000 V ...
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bioRxiv - Cancer Biology 2020Quote: siRNA transfections for CXCR1 and CXCR2 oligonucleotides (Dharmacon, Lafayette, CO, USA) were carried out using Lipofectamine® RNAiMAX Transfection Reagent (Life Technologies, Paisley, UK) when cells reached 60-70% confluence ...
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bioRxiv - Cell Biology 2022Quote: siRNA transfections were carried out using 20 pmol of siRNA (SI Table 1) targeting LTBP3 (siLtbp3-1-4) and lipofectamine RNAiMAX (Invitrogen, Waltham, MA; 13778-075) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... HAUS6 and HAUS8 siRNA transfections were performed 48 hours prior to imaging and cotransfection with 100 nM Mad2 siRNA was additionally performed 24 hours prior to imaging using Lipofectamine RNAiMAX Reagent (Life Technologies, Waltham, MA, US) according to the instructions provided by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.3 to 1.75 x 105 cells/per well were seeded in 12-well plates adding a mixture that was pre-incubated at room temperature for 20 minutes and contained 2 µl of lipofectamine and 1 µl of 20 µM of siRNA in 200 µl of Opti-MEM™ (Gibco, Catalog no. 31985062). The mixture was added to 800 µl of complete medium and cells.
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bioRxiv - Cell Biology 2022Quote: ... confluent pre-adipocyte (day -2) 3T3-L1s grown in 12-well plates were transfected with a pool of two anti-Mfn1 siRNAs (ThermoFisher Scientific, CatIDs: s85002 & s85004), each at 40nM to achieve a final concentration of 80nM ...
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bioRxiv - Cell Biology 2024Quote: ... 10% HIFBS) and reverse transfected with 1 pmol/well of a combination of 3 non-overlapping 11-mer siRNAs (Ambion Silencer® Select, Thermofisher) using Lipofectamine RNAiMax transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: A total of 5 × 104 MCF7 cells were seeded on coverslips and transfected with siRNAs (10 nM) using Lipofectamine RNAiMAX (Thermo Fisher Scientific, MA, USA) by reverse transfection according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... AHR- or OVOL1-specific siRNA (generated by Huzhou Hippo Biotechnology Co., Ltd) was transfected into NHEKs using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific, Cat# 13778075) per manufacturer’s protocol.
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... siOTULIN#3, s40308 (20 nM each, 60 nM in total) and a Silencer™ Select negative control siRNA (Thermo Fisher Scientific, #4390843, 60 nM). For reverse transfection ...