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Citations for Thermo Fisher :
3001 - 3050 of 3282 citations for GPX6 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... PNKP expressing stable cell lines were transfected with 10 μM PNKP 3’-UTR specific siRNA (Horizon Discovery) in a 24-well plate using RNAiMAX (ThermoFisher Scientific) according to the manufacturer’s protocol to deplete endogenous PNKP ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of 5 µM targeting or non-targeting siRNAs were mixed with 190 µl reduced serum medium (Opti-MEM™, Gibco). In a separate tube ...
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bioRxiv - Immunology 2023Quote: ... Transfection mix was gently mixed by pipetting and then incubated at room temperature for 20 minutes.The lipid-siRNA complexes were added to 10×106 monocytes in 1.5 mL of Macrophage-serum free media (Thermo Fisher Scientific) and incubated for 4 hours at 37°C with a gently rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... L-047399-01-0005) or Scramble (Dharmacon, ON-TARGETplus Non-targeting Control siRNA, D0018100205) at 200 nM final concentration using Lipofectamine 3000 (ThermoFisher, L3000001) in Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: MA104 cells seeded at a density of 5×105 in a 60 mm tissue culture plate were reverse transfected with 10 nM of indicated siRNA and 10 µl Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific) in a final volume of 5 ml as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected with the TRIB1 targeting (L-003633-00-0010) or non-targeting control (D- 001810-10-2) ON-TARGETplus siRNA SMARTpools (Dharmacon) using RNAiMAX (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The siRNA was reverse transiently transfected into SK-MEL-28 and SK-MEL-2 cells using Lipofectamine RNAiMAX® (Life Technologies) following the manufacturer’s instructions and incubated for 72 h/37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... or an Alexa Flour non-targeting control siRNA were transfected at 25nM into the HMLE cell line using Lipofectamine RNAiMAX (Thermo Scientific). Cells were seeded at 3000 cells per well into a 96-well plate during reverse transfection ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded onto coverslips at 30-40% confluency and transfected with 50 nM of siRNA in two sequential transfections using Lipofectamine RNAiMAX (Life Technologies) in OPTI-MEM (Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Alternatively, cells were transfected with siRNAs against SCAF1 (Dharmacon, ON-TARGET plus SMARTpool; Horizon Discoveries, UK) using RNAiMax (ThermoFisher Scientific, USA). Clonogenic survival assays were seeded 48 hr post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... L-005139-00-0005) or a non-targeting control siRNA (Dharmacon, D-001810-10-05) by using Opti-MEM (Gibco, 31985062) and the TransIT-X2® Dynamic Delivery System (Mirus ...
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bioRxiv - Cell Biology 2023Quote: ... MCF-10A cells were transfected with a final concentration of 50 nm of siRNAs diluted in Opti-MEM® Reduced Serum Medium (Gibco) using LipofectamineTM RNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... KIF18A siRNA used was a 1:1 mixture of the following two Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334) CGUUAACUGCAGACGUAAAtt (Ambion ...
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bioRxiv - Developmental Biology 2023Quote: ... Transfection with SMARTPool ON TARGETplus Pou5f1/Oct4 siRNA (Horizon Discovery) and ON-TARGETplus Non-targeting Control siRNAs Control #1 (Horizon Discovery) was performed using Lipofectamine RNAi MAX (Life Technologies) at a final concentration of 100nM ...
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bioRxiv - Cancer Biology 2023Quote: NOF and CAF seeded into 6-well plates were transfected at ∼60-70% confluence by diluting siRNAs in serum free medium in the presence of Oligofectamine 2000 transfection reagent (Invitrogen, 12252011) following the manufacturer’s recommendations ...
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bioRxiv - Pathology 2023Quote: ... 25 nM) or Human YAP/TAZ-siRNA (UGUGGAUGAGAUGGAUACA) (102) by using Lipofectamine RNAiMAX reagent (13778075, Thermo Fisher Scientific, Waltham, MA, USA). Cells were harvested after 72 h.
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bioRxiv - Molecular Biology 2023Quote: ... s70523), and p62 (si-p62, s71144) and their control siRNA (Silencer select negative control No. 1) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the transfection mix was diluted 10 times (to a final concentration of siRNA of 2 nM) in MEMalpha GlutaMax medium (Gibco #32561037) supplemented with 2.5% human platelet lysate (Sigma #SCM152) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with siRNAs or microRNA mimics at 30 nM or as otherwise indicated using Lipofectamine RNAiMAX (Invitrogen 13778-150) and Opti-MEM (Gibco 31985070 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were reverse-transfected into an arrayed 384- well plate library containing small interfering RNA (siRNA) or miRIDIAN microRNA mimic constructs (Horizon Discovery, Supplementary Tables 1-7) using Opti-MEM (Gibco 31985070) and Lipofectamine RNAiMAX transfection reagent (Invitrogen 13778150) ...
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bioRxiv - Molecular Biology 2024Quote: VSMCs were transfected with Small interfering RNA (siRNA) against Id2 (RiboBio, Guangzhou, China) using Lipofectamine RNAiMAX Reagent (Invitrogen, Carlsbad, CA, USA). The target sequence of mouse siRNA-ID2 was 5’-GACCCAGTATTCGGTTACT -3’ ...
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bioRxiv - Microbiology 2023Quote: Vero-E6 cells growing in 12 well plate after siRNA transfection and/or infection with CHPV were lysed in 1ml Trizol (cat no.15596026, Invitrogen/ Thermofisher scientific), 200μl of chloroform was added and mixed vigorously for 15 sec and incubated at RT for 2-3 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... and reverse transfected with 30 nM siRNA using DharmaFECT 1 Transfection Reagent (Horizon, T-2001,03) according to manufacturer’s instructions (using Opti-MEM™ I (ThermoFisher Scientific, 11058021) and antibiotic free media) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded into 6-well plates 24 h prior to transfection, 50 nM siRNA and 8 μL INTERFERin (Polyplus, France) were mixed in Opti-MEM (Gibco, USA), incubated for 10 min at room temperature and added to the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... KIF18A siRNAs used were a 1:1 mixture of two the following Silencer or Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334), GCUUGUUCCAGAAUCGAGAtt (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: ... HeLa Kyoto and RPE1 cells were treated with 5 pmol siRNA that was preincubated for 5-10 minutes in Opti-MEM Reduced Serum Media (Gibco, 31985062) with Lipofectamine RNAiMax Transfection Reagent (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Silencer Select siRNAs of Rheb (s12019, s12020, s12021), Rac1 (s11711, s11713), and Cdc42 (s2765, s2766, s2767) were purchased from Thermo Fisher. Dock7 shRNAs and negative control were purchased from Sigma (shRNA1 ...
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bioRxiv - Microbiology 2024Quote: dHepaRG cells or PHHs seeded into a 24-well plate were transfected with 25 nM of siRNA using Lipofectamine RNAiMax (Life Technologies), following manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... transfected with 5 pmol/well of the Silencer Selected siRNAs specific to the mRNA of SLC35G1 (Thermo Fisher Scientific, Waltham, MA), using 1.5 mL/well of Lipofectamine RNAi MAX (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µL gene-specific siRNA oligomer (20 µM) was diluted in 49 µL Opti-MEM reduced serum medium plus GlutaMAX (ThermoFisher Scientific) and mixed with 3 µL of Lipofectamine RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Non-targeting siRNAs control (Scramble controls) and SOX2/USP9X/IGSF1/CHRDL1 siRNA were transfected into cells by using Lipofectamin RNAiMAX Transfection Reagent (Invitrogen, 13778075). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... or single or combination of Drp1 variant specific siRNA oligonucleotides against target sequence listed in Table 2 using Lipofectamine RNAiMAX (Invitrogen, 13778150). 48 hours post-transfection cells were seeded for experiments ...
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bioRxiv - Cell Biology 2024Quote: The siRNA transfected cells were dissociated by trypsin and counted using trypan-blue solution and an automated cell counter (Invitrogen, Countess). For each spheroid ...
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bioRxiv - Microbiology 2024Quote: ... cells or PHHs seeded into a 24-well plate were transfected with 25 nM of siRNA using Lipofectamine RNAiMax (Life Technologies), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfections were performed in fibroblasts using 5nM final concentration of siRNA and in hESCs using a 10nM final concentration of siRNA and 2.5ul/ml final concentration of Lipofectamine RNAiMAX (Thermo Fisher #31985070).
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bioRxiv - Cancer Biology 2024Quote: ... each well was transfected via master mix 3 µL of siRNAs (20 µM) via 9 µL Lipofectamine 2000 (Invitrogen, #.11668-019) in 300 µL OPTI-MEM (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... cells were plated at 15% to 20% confluence in 6-well plates together with 15 nM Silencer Select or Silencer Pre-designed siRNA and 3.5 µL of RNAi MAX (Thermo Fisher Scientific), or with 150 pmol of Stealth siRNA and 3.5 µL of RNAi MAX ...
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bioRxiv - Genomics 2024Quote: Knockdown of ERG was performed as previously described (41) using 1 nM siRNA oligonucleotides in OptiMEM (ThermoFisher Scientific, cat. no. 11058021) with Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: SH-SY5Y cells were seeded in 96-well plates and transfected with 1pmol siRNA according to Lipofectamine™ RNAiMAX transfection reagent (Invitrogen) manufacturer protocol at 48 hours post-transfection relative growth rate was measured ...
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bioRxiv - Cancer Biology 2024Quote: ... CHLA-42 cells were seeded in 6 well plates and transfected with 25pmol H19 siRNAs according to Lipofectamine ™ RNAiMAX transfection reagent (Invitrogen) manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: U2OS cells were reverse transfected with 25 nmol siRNA targeting the 3′ UTR of FANCM (sequence: AAAGACCUCUCACAAUAUUtt from (25)) using lipofectamine RNAiMAX (ThermoFisher Scientitifc), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: 1x 105 HeLa-GL cells/well were grown in 500 µL medium in 24-well plates and one day later transfected with siRNA using Lipofectamine RNAiMax Transfection Reagent (Invitrogen, #13778150) and Opti-MEM reduced serum media according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... PASMCs were transfected with 5 pmol of each siRNA using Lipofectamine RNAiMAX transfection reagent (cat # 13778-150, Life Technologies; Carlsbad, CA). After overnight transfection ...
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bioRxiv - Cell Biology 2024Quote: For gene silencing, cells were transfected with the Stealth™ RNAi (12935300, Med GC) as a negative control and siRNA (Invitrogen) to silence TMX2 or TOM70 expressions (siTMX2-1 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected at 12 and 24 hours post infection with 25 pmol/well siRNA (Silencer select, Ambion distributed by Thermo Fisher) (Table 3 ...
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bioRxiv - Cell Biology 2024Quote: ... ON-TARGET plus mouse PAK3 SMART pool siRNAs or scrambled were transfected 24 hours later to the same cells using Lipofectamine 3000 (Life Technologies). The final siRNA concentration was 70 nM/sample.
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with a final concentration of 10nm siRNA targeting the gene of interesting or a scrambled control using Lipofectamine RNAiMAX according to manufacturers’ instructions (Life Technologies, 13778030). The following siRNAs were used ...
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bioRxiv - Cell Biology 2020Quote: ... or a 1:1 mixture of two siRNA to CHMP2A (5’-CAGGCCGAGAUCAUGGACAUG-3’ and 5’-GAAGAUGAAGAGGAGAGUGAC-3’) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and fed with complete Lifeline medium with antibiotics and transfected with 100 nM 5′ tRNA-fMet1 half (#10620310, Invitrogen custom siRNA, Thermo Fisher Scientific) or 100 nM AllStars Negative Control siRNA (siNC ...
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bioRxiv - Molecular Biology 2021Quote: ... and a human IL-6 siRNA pool (Horizon Discovery, LQ-007993-00-0005) were transfected using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, 13778) according to the manufacturer’s protocol ...