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Citations for Thermo Fisher :
3151 - 3200 of 3282 citations for GPX6 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... H295R cells were seeded in 6-wells plate at a density of 0.3×106 cells/well and transfected the day after with a CTNNB1 siRNA (Thermofisher, Carlsbad, USA, #s436) or a control siRNA (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... ON-TARGETplus Mouse Dlg1 SiRNAs (SMARTpool, L-042037-00-0005 were used for Unc5B gene deletion. Transfection was performed using lipofectamine RNAi max (Invitrogen, 13778-075) according to the manufacturer’s instruction with siRNA at a final concentration of 25pmol in OptiMem for 8h ...
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bioRxiv - Developmental Biology 2023Quote: ... For RNAi YAP and TAZ knock down – hMSCs were starved for 6h in DMEM without antibiotics before adding siRNA for YAP or TAZ and scramble control (Stealth RNAi Invitrogen Thermo Fisher Scientific) in OptiMEM with addition of Lipofectamine 3000 (both Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... or siRNA explants were incubated with 10,000 molecular weight (MW) neutral Dextran Texas Red™ (25 μg/mL; D1828; Invitrogen, Eugene OR) for 25 minutes in IMDM+0.1% BSA and washed with cold PBS before fixation with 4% paraformaldehyde for 2hrs on ice.
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bioRxiv - Molecular Biology 2023Quote: ... they were transfected at 40% confluency with 200 ng of the pcDNA-MATR3 vectors and 10 μM of either control or MATR3 siRNA per well using the Lipofectamine 3000 transfection reagent (ThermoFisher Scientific L3000015). ON-TARGETplus non-targeting siRNA #1 ((Dharmacon D- 001810-01-05) ...
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bioRxiv - Physiology 2024Quote: ... R-5’-3’ UGGUUCAUCAGCCUUUAACTT) and mock siRNA (General Biosystems, China) were transfected into mouse DCT cells by Lipofectamine™ 3000 (Invitrogen, L3000015) (20 nM ...
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bioRxiv - Microbiology 2023Quote: Vero-E6 cells growing in 12 well plate after siRNA transfection and/or infection with CHPV were lysed in 1ml Trizol (cat no.15596026, Invitrogen/ Thermofisher scientific), 200μl of chloroform was added and mixed vigorously for 15 sec and incubated at RT for 2-3 mins ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: ... and CIC siRNA (#L-015185-01-0005) were obtained from GE Dharmacon and transfection was performed with Lipofectamine RNAiMax transfection reagent (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... SKOV-3 cells were transiently transfected with either 100nM siRNA or 2μg plasmid DNA by electroporation using the Neon transfection system (Thermo Fisher Scientific, UK) using the following parameters ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were co-transfected with pcDNA3.1_VSVG-EYFP_ts045 (Yonemura et al., 2016) and the indicated plasmid / siRNA using Lipofectamine™ 3000 (Thermo Fisher Scientific, cat.no. L3000015) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... while in a separate tube the appropriate amount of each siRNA was added to appropriate volume of OptiMEM (Gibco, cat no. 31985070) and mixed ...
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bioRxiv - Cell Biology 2023Quote: ... and siRNA (corresponding to the shRNA sequences detailed below) were then introduced to the cells following the manufacture protocol (Thermo Fisher Scientific). The cells were left to incubate for 48 hours.
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bioRxiv - Cancer Biology 2023Quote: siRNA screening was performed in MCF-7 TEAD reporter cells using two different siRNA libraries (Dharmacon On-Target Plus and Ambion Silencer Select) targeting ubiquitin-related genes ...
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bioRxiv - Cell Biology 2024Quote: ... The siRNAs were delivered to HEK293FT cells by bPEI or HeLa and MDA-MB-231 cells by RNAiMAX reagent (#13778150, Thermo Fisher Scientific) KD efficiency was determined by WB ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of HeLa and HEK293 cells with siRNAs (100 pmol each) was performed using Lipofectamine RNAi max (Invitrogen-Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of HeLa and HEK293 cells with siRNAs (100 pmol each) was performed using Lipofectamine RNAi max (Invitrogen-Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cells with 60%-70% confluency were seeded 16 hours prior to the experiment and 1.5 μM siRNA were mixed with 9 μl Lipofectamine RNAiMax (Thermo Fisher Scientific, USA) in OptiMEM according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or 10 nM siRNA (for ELOB and ELOC knockdown) in two consecutive days using the Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: SKMEL-23 and SKMEL-1128 cells were transfected with 25 nM and 50 nM siRNA respectively using lipofectamine RNAiMAX (Thermo Fisher Scientific). Silencer select negative control siRNA#1 (4390843 ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Cancer Biology 2024Quote: SNU1967 cells were transfected with ON-TARGETplus Human siRNA SMARTpool targeting SOX9 (L-021507-00-0005) using Lipofectamine RNAiMAX (Thermo Fisher Scientific). Post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected at 12 and 24 hours post infection with 25 pmol/well siRNA (Silencer select, Ambion distributed by Thermo Fisher) (Table 3 ...
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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bioRxiv - Molecular Biology 2024Quote: ... with 100 nM of either ON-TARGETplus Non-targeting Control siRNA Pool (Dharmacon #D-001810-10-05) or On-TARGETplus Smartpool siRNA targeting rat ACLY protein (Dharmacon #L-089773-02-0005) using lipofectamine RNAimax (Thermofisher Scientific #13778075), as per manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... LAK cells were transfected with 60 pmol of siRNA oligonucleotides specific to NMNAT1 and NMNAT3 using Lipofectamine RNAi/MAX (Invitrogen, Carlsbad, CA, USA) without antibiotics according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2019Quote: ... oligonucleotide duplexes were designed against TG2 (sense, 5’-AAGGGCGAACCACCTGAACAA-3’ and antisense, 5’-TTGTTCAGGTGGTTCGCCCTT-3’) and TOPOIIα siRNA (purchased from Thermo Fisher, Catalog # AM16708). Plasmids and miRNA were transfected with Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... The transfection solution was 1µg/µl siRNA, 10% polyethylene glycol (PEG) (Carbowax 6000, Union Carbide) and 20% TurbofectTM (Thermo Fisher Scientific, Catalog # R0541). For 2.8µl of siRNA preparation ...
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bioRxiv - Biochemistry 2021Quote: ... A673 cells (5.0 × 105) in 6-well dishes were reverse transfected with siRNA (50 nM) using Lipofectamine™ RNAiMAX (Invitrogen, cat. no.13778075) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: Cells were plated at 40% confluency in a single well of a 24-well plate with 30 μM total siRNA (ThermoFisher Silencer Select #4390771) and 3 μl RNAiMAX (Invitrogen) ...
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bioRxiv - Pathology 2021Quote: ... or LGALS1 siRNA (Eurofins Genomics; Ebersberg, Germany) with the sense sequence 5’-[UUGCUGUUGCACACGAUG-GUGUUGG]-3’ the following day using Lipofectamine® RNAiMAX (Thermo Fisher Inc) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... White hMADS were transfected at day 12 of differentiation with 30 nmol of siRNA or ASO at day 10 using Lipofectamine™ RNAiMAX transfection reagent (Thermo Scientific, #13778075) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: A549 cells were transfected for 24h with RP-targeting or non-targeting (control) siRNAs at a final concentration of 40nM using Lipofectamine RNAiMAX reagent (Invitrogen, catalog no.13778150) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: The whole genome siRNA screen was conducted as described (35) using HeLa S3 cells and the Dharmacon ON-TARGETplus® SMARTpool® human siRNA library (Thermo Scientific) and strain T3SA+.
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bioRxiv - Cell Biology 2021Quote: ... Dharmacon L-006009-00-0010) or control non-targeting siRNA (25 or 50 nM; Dharmacon; Cat. No. D-001810-10-20) using Oligofectamine (Invitrogen, Cat. No. 12252011) as we described56.
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNAs targeting human SNX13 (5’- CAGAAAGGCUCAACAGAAAUU-3’) or SNX14 (5’-GGAUGAAAGUAUUGACAAAUU-3’) using Lipofectamine RNAiMax (Invitrogen, Cat#13778-075) according to the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... siRNA oligonucleotides were obtained as pre-designed siRNAs as follows: MFF-sense strand: 5’-CGCUGACCUGGAACAAGGAdTdT-3’ for exon 2 30 (Ambion, Austin, TX, USA); DLP1-sense strand ...
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bioRxiv - Cell Biology 2022Quote: ... 21-nucleotide small interfering RNA (siRNA) duplexes were transfected into MEFs MAVS-PEX and MEFs MAVS-MITO cells using Lipofectamine RNAiMAX (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Transfections with either pCMV or pTRIP TRE Bi plasmids and siRNAs were performed through electroporation using the Neon Transfection System (Thermo Fisher Scientific MPK5000). For pCMVs ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... cells were transfected with either targeting or non-targeting siRNA constructs which were diluted in OPTI-MEM medium (Life Technologies, Waltham, MA, USA) to a final concentration of 100 nM in the medium with cells ...
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bioRxiv - Microbiology 2021Quote: ... Each siRNA including negative control siRNA at an amount of 20 pmol was transfected into cells with 6 µL Lipofectamine RNAiMAX Reagent (Life technologies, 13778-150). 24 h later ...
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bioRxiv - Cell Biology 2022Quote: ... mDia2, FMNL2, mDia1, DAAM1, VASP, fascin, and myosin-X (Dharmacon, ON-TARGET plus SMART pool siRNA) using Lipofectamine RNAiMAX (ThermoFisher, catalog number: LMRNA015) on day 1 and day 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.3 million cells were harvested and reverse transfected with a specific siRNA duplex at 25 nM using Lipofectamine RNAiMAX Reagent (Invitrogen, Cat. No.13778075) according to the manufacturer’s instructions for 48 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (n = 500 000 cells/well) were transfected with 25 nM of each siRNA individually using Lipofectamine® 3000 Transfection Reagent (ThermoFisher Scientific, # L3000075) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... As a control, siGENOME Non-Targeting Pool #1 (Dharmacon, D-001206-13-05) and a second independent predesigned PURA siRNA (Thermo Fisher Scientific, 289567) was used as described for the PURA siRNA pool ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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Endometrial Gap Junction Expression - Early Indicators of Endometriosis and Integral to InvasivenessbioRxiv - Cell Biology 2021Quote: ... we subsequently shifted to 24 hour pre-treatment of the LP9 monolayer with a combination of two siRNAs to Cx43 (10 pmoles/well or 5nM final concentration) - Ambion™ Silencer™ Select) in Opti MEM (Gibco, NY) with RNAi MAX (1/100 dilution ...
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bioRxiv - Cell Biology 2020Quote: For Western blot analysis after esi/siRNA or chemical treatment cells were washed with PBS and directly lysed in 1x NuPAGE LDS buffer (Thermo Fisher Scientific, NP0008). For immunoprecipitation (IP ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were transfected with siLgr6 or siCon siRNAs (100nM or as noted) using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher, Waltham, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The siRNA-resistant form of RAB10 was generated by introducing 3 silent mutations into the target sequence of the seed region of the RAB10-siRNA (Ambion, Thermo Fisher, ID s21391), altering the original sequence by site-directed mutagenesis (QuickChange ...
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bioRxiv - Genetics 2021Quote: ... 250 000 cells in 2 ml of medium in a six-well plate were transfected using 12.5 pmol of Silencer Select siRNA against human ADCY9 (Ambion cat # 4390826 ID 1039), Silencer Select siRNA against CETP (Ambion cat 4392420 ID 2933 ...