Labshake search
Citations for Thermo Fisher :
2751 - 2800 of 3282 citations for GPX6 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... 60 pmol of siRNA was mixed with 1.5 μl Lipo3000 in 50 μl of serum-free OPTIMEM (Gibco Life Technologies) for 15 min at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... 60 pmol of siRNA was mixed with 1.5 μl Lipo3000 in 50 μl of serum-free OPTIMEM (Gibco Life Technologies) for 15 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... 0.1 and 1 μM MARCH8-specific siRNA or non-targeting control (NTC) (Smart pool, Dharmacon) using Lipofectamine RNAiMAX (ThermoFisher Scientific) following the manufactuer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... 25 pmol of siRNA and 3 μL of RNAiMAX transfection reagent was incubated in 500 μL Opti-MEM (Thermo Scientific) and then added to cells in 2 ml media without antibiotics ...
-
bioRxiv - Molecular Biology 2020Quote: ... and ON-TARGETplus SMART pool siRNA oligonucleotides and ON-TARGETplus NON-targeting pool (D-001810) (Dharmacon) were transfected with 25 or 50 nM siRNA by Lipofectamine RNAimax (Invitrogen) for 48 to 72 h following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... HeLa or 293T cells were transfected with a 30 nM final concentration of siRNA using the Lipofectamine RNAiMax (Life Technologies). 48 hrs post-transfection ...
-
Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Human lung fibroblasts were transfected with the indicated siRNA at a final concentration of 35 nM using Lipofectamine RNAiMAX reagent (Invitrogen).
-
bioRxiv - Cell Biology 2021Quote: ... the Opti-MEM I medium was replaced by DMEM growth medium containing 25 nM siRNA and 1.6 μl/ml Lipofectamine 3000 (Life technologies) and incubated overnight ...
-
bioRxiv - Cell Biology 2021Quote: MIN6 cells were resuspended with 25 nM antiTMEM24 siRNA smartpool (Dharmacon, siGENOME) pre-mixed with 1.6 μl/ml Lipofectamine RNAiMAX (Life technologies) in Opti-MEM I medium ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were transfected with siHMOX2 (Dharmacon L-009630-00-0005) or non-targeting pool (Dharmacon D-001810-10-05) siRNA using Lipofectamine RNAi Max (Thermofisher). Briefly ...
-
bioRxiv - Genomics 2021Quote: 1 μM concentration of siRNA targeting ESR1 gene and non-targeting control (NTC) were mixed with Opti-MEM (Life Technologies) and 1 μl of Lipofectamine RNAiMax to a total volume of 100 μl in a 24-well plate ...
-
bioRxiv - Genomics 2021Quote: ... the cells were transfected with 50 nM of siRNA against Exosc10, Rad21, or Egfp using DharmaFECT 1 (T-2001-03, Dharmacon) and Opti-MEM (Gibco) according to the manufacturer’s protocols ...
-
bioRxiv - Cell Biology 2021Quote: HUVECs were plated in 6-well tissue culture plates and transfected with siRNA at a 50 nM concentration with RNAiMAX (ThermoFisher) using the protocol provider by the manufacturer ...
-
bioRxiv - Cancer Biology 2021Quote: ... Silencing of Cdkn1a in Hepa cells was achieved by siRNA (Supplementary Table S6) transfection using with Lipofectamine RNAiMAX from Invitrogen. Total glutathione (GSH ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with either non-targeting control (Dharmacon, D-001810-10-05) or Mage-b4/10 siRNA oligonucleotides by Lipofectamine 3000 (Invitrogen) as follows ...
-
bioRxiv - Cancer Biology 2022Quote: ... and expression/empty vector or 0.55 ul of 20µM corresponding siRNA in 24-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... was obtained from Addgene (a gift from Harald Stenmark). Silencer Select siRNA targeting IFITM3 (s195035) and a non-targeting control (No. 1) was obtained from Ambion. Cells were transfected with 20 nM siRNA using Opti-MEM (Gibco ...
-
bioRxiv - Microbiology 2022Quote: ... and eight plates were transfected with pooled siRNAs against NTC (Dharmacon) according to the manufacturing protocol for RNAiMAX (ThermoFisher Scientific). At 48 hours post-transfection ...
-
bioRxiv - Cancer Biology 2022Quote: ... individual ON-TARGET PLUS siRNAs were used at a final RNAi concentration of 40nM and was added to 5μL of RNAiMAX (ThermoFisher, 13778150) and 500μL Hank Buffer Salt Solution without sodium bicarb ...
-
bioRxiv - Cancer Biology 2022Quote: RICTOR knockdown experiments were performed using On-TARGETplus Smartpool Human RICTOR siRNA or On-TARGETplus Non-targeting Control Pool (Dharmacon) were transfected into U87MG cells using LipofectamineTM 3000 Transfection Reagent (ThermoFisher) in Opti-MEM media (Gibco) ...
-
bioRxiv - Biochemistry 2022Quote: ... targeting the transcript of interest (see Supplementary Table S1) or non-targeting siRNA as control by using Lipofectamine RNAiMax (Invitrogen) as transfection reagent ...
-
bioRxiv - Cancer Biology 2022Quote: ... 500,000 RPE1-ST-1 cells previously infected with the TaqMan-TS-sgTS lentivirus were reverse-transfected with Silencer Select siRNAs (ThermoFisher) in using Lipofectamine RNAiMAX (ThermoFisher) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and CAL-51 cell lines were transiently transfected with siRNAs and sense/antisense oligonucleotides (S6 Table) using Lipofectamine 2000 (Invitrogen). After 4 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... 5′-CCUGGAUAAUGAUGAAGGA-3′), OSBP (predesigned, cat. no. 4392420) and nontargeting control siRNA (predesigned, cat. no. 4390844) were purchased from Ambion. ON-TARGET plus Human PI4K2A siRNA (predesigned ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells at 50% confluency were transfected with 20–40 nM final siRNA concentration using Lipofectamine RNAiMax transfection reagent (Life Technologies) according to the manufacturer’s instructions and harvested after 48 h.
-
bioRxiv - Cell Biology 2022Quote: ... Mouse pancreatic islet cells suspended in 1 × 105 cells/280 μl were transfected with 100 nmol/L siRNA using Lipofectamine RNAiMAX reagent (Invitrogen). After plated on a 24-well plate or glass base dish for 48 h ...
-
bioRxiv - Cell Biology 2022Quote: Silencing interventions in THP-1 and WI-38 cells were performed by transfecting 50 nM of siRNAs using Lipofectamine (RNAiMax, Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... MDM were seeded at 1×105 cells/well and transfected with 60 nM total Silencer Select NHLRC2 (20 nM each of s51529, s51530 and s51531) siRNAs (Ambion) according to the method developed by Fisch et al ...
-
Long Noncoding RNA MALAT1 is Dynamically Regulated in Leader Cells during Collective Cancer InvasionbioRxiv - Cancer Biology 2022Quote: ... 20 nM siRNAs were transfected into the cells by Lipofectamine RNAiMAX according to the manufacturer’s protocol (Fisher Scientific, Hampton, NH) for 48 hours ...
-
bioRxiv - Physiology 2022Quote: ... R:3’-UUGAACGUCACUAUAUUAACUGUUGUA-5’) dicer-substrate siRNA (DsiRNA) (20 nM, Integrated DNA Technologies, Coralville, IA) using Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Mix 1 was prepared by adding 30 pmol of siRNA to 150 µl non-supplemented Opti-MEM medium (Thermo Fisher) and then gently mixing ...
-
bioRxiv - Physiology 2022Quote: ... LLC-AQP2 cells were cultured to 50% confluency and transfected with 20 nM siRNA targeting RSK1 (Invitrogen, Charlestown, MA, USA) 5’-GCCACGAAAGCAUGGUUUAUCUAAA-3’ (RSK siRNA1 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were transfected in the presence of 10 nM of either siRNA or control RNA with Lipofectamine RNAiMAX reagent (Invitrogen) in a final volume of 0.5 mL of OPTI-MEM ...
-
bioRxiv - Molecular Biology 2022Quote: ... U2OS cells were seeded and EME1 knockdown was performed using siRNA of EME1 with Lipofectamine RNAiMAX transfection reagent (LMRNA015, Invitrogen) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2×105 cells were transfected with 20 pmol of ON- TARGETplus Human HMG20A (10363) siRNA-SMART pool (Dharmacon) using Oligofectamine™ according to the manufacturer’s instructions (Invitrogen). Cells were cultured for 3 days before being harvested for follow-up experiments.
-
bioRxiv - Molecular Biology 2022Quote: ... all siRNAs were transfected at a concentration of 25 nM in a forward transfection for 48 hours using RNAimax (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... oligonucleotides with an siRNA sequence were cloned into the BamHI and HindIII sites of pSilencer 2.1-U6 neo Vector (ThermoFisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Physiology 2022Quote: ... BM-derived DCs were transfected with ON-TARGET SMART pool of 4 siRNA to mouse Tlr2 (50 nM, Dharmacon, Horizon Discovery, USA) using lipofectamine RNAiMAX (Invitrogen), and were further differentiated into OCLs for 5-6 days either in OCL differentiation medium in the presence of the indicated concentration of agonists ...
-
bioRxiv - Molecular Biology 2024Quote: The transfection of a mixture of specific siRNA against c-Myc protein (exon 2 and exon 3, IDs s9129 and s9130, respectively; Ambion) and DOT1L protein (IDs s39011 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were grown in 60 mm cell culture dishes in medium supplemented with 2% FBS overnight and transfected with 62.5 pmol of the indicated siRNAs applying Lipofectamine RNAiMAX (Invitrogen, #13778150). After 48-72 h ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were transiently transfected at 50-60% confluency with plasmid expressing protein of interest (Table 1) and/or target specific siRNA using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: The siRNA (GenePharma) used to knock down the expression of USP8 was transfected into cells by using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Rab8b-specific SMARTpool siRNAs (L-012116; L-004374; L-026032; L-019045; L-003905; L-008744; Horizon Discovery) using Lipofectamine RNAiMAX reagent (Invitrogen). Media was refreshed 24 h post-transfection ...
-
Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... Cells were treated with one of three different commercially validated AREG targeting siRNA (Ambion, see oligo sequences on Table S5) or a negative control siRNA (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... THP-1 were seeded in twenty-four-well plates for 24 h in the presence of 200 nM of PMA then transfected with 50 nM siRNA using Lipofectamine RNAIMAX (Invitrogen) according to the manufacturer’s instructions and incubated for a further 3 days ...
-
bioRxiv - Cell Biology 2023Quote: ... To deplete LaminA from RPE-1 cells were transfected with small interfering RNAs (siRNAs) using Lipofectamin RNAi Max (Life Technologies). Specifically ...
-
bioRxiv - Cell Biology 2023Quote: ... siRNA-mediated depletion of endogenous ESCO2 was done with 20nM siRNA (Dharmacon, J-025788-11, target nucleotides: GUUGAUACCCUCAGGAAUU) in serum-free Opti-MEM (Gibco) 24 hours after induction ...
-
bioRxiv - Cell Biology 2023Quote: ... After 24h cells were transfected with 50nM SMARTpool siRNA in Nutristem medium without antibiotics together with RNAiMAX (Thermo Fisher Scientific), according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: 293T IFNB12X-GLuc cells plated in 96-well plates were transfected with 1 pmol/well siRNA (Silencer Select, Thermo Fisher) using Lipofectamine RNAiMAX (Thermo Fisher ...
-
bioRxiv - Cell Biology 2023Quote: ... The expression of TLNRD1 was suppressed in HUVECs using 50 nM siRNA and Lipofectamine™ RNAiMAX (Thermo Fisher Scientific, 13778075) following the manufacturer’s instructions ...