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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were measured using Pierce BCA protein assay kit (Thermo Fisher). Samples were denatured and proteins were separated by SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... We performed two washes with 1% BSA (bovine albumin serum) PBS (phosphate-buffered saline) containing 50 U/ml of SUPERase-In (Thermo Fisher, cat# AM2696) and 50 U/ml of RNasin (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... Cholera toxin subunit B coupled to Alexa 555 (CTB555, ThermoFisher, c34776) was injected medially was injected medially (Ctl - AP +1.0 ...
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bioRxiv - Neuroscience 2024Quote: ... and 100 µg/ml streptomycin (# 15140148, Thermo Fisher Scientific), and if required 1% non-essential amino acids (# 11140050 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA levels were normalized to mouse Gapdh or human RPL22 cDNA and relative transcription levels were analyzed using the comparative ΔΔCt method (7500 Software V2.0.5, Applied Biosystems, Life Technologies). The Lgmn mRNA level of the microglia-enriched fraction was obtained from a NanoString dataset (Omnibus GSE129709 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE129709)10.
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bioRxiv - Neuroscience 2024Quote: ... Isolated cells were washed twice with D-PBS (# 14040-133, Thermo Fisher Scientific) to remove BSA ...
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bioRxiv - Neuroscience 2024Quote: ... carbogen-bubbled ACSF with the addition of 15uL of 5mg/mL Di-4-ANNEPS (Thermofisher #D1199) in high purity ethyl alcohol (EtOh) ...
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bioRxiv - Neuroscience 2024Quote: ... The female iPSC line A18944 was purchased from Thermo Fisher (# A18945), grown in Essential 8 Flex Medium (# A2858501 ...
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bioRxiv - Neuroscience 2024Quote: ... on VTN-coated (# A14700, Thermo Fisher Scientific) cell culture plates with 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The hippocampi were isolated and dissected in ice-cold dissection buffer (HBSS, 1 mM sodium pyruvate, 10 mM HEPES pH 7.2, all from Thermo Fisher Scientific). Single cell suspension was obtained by pelleting the tissue (300 x g ...
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bioRxiv - Neuroscience 2024Quote: ... and if required 1% non-essential amino acids (# 11140050, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using SuperScript IV (Invitrogen) and gene-specific primers (Table S4) ...
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bioRxiv - Neuroscience 2024Quote: ... Dharmacon) and mock reverse transfections were carried out with LipofectamineTM RNAiMAX (# 13778030, Thermo Fisher Scientific). For each 10 cm dish 3 µl siRNA (20 µM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Staining with DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, 701629) at 1:500 dilution was performed in blocking solution at 4℃ for 14 hours ...
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bioRxiv - Synthetic Biology 2024Quote: ... Compensation was carried out using UltraComp eBeads™ Compensation Beads (Invitrogen; Cat. No. 01-2222-42).
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 % Pen/Strep (Thermofisher Scientific; Cat. No. 15140122) or Antibiotic-Antimycotic (Thermofisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 10% goat serum (Thermo Fisher Scientific, 16210072) for 40 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Total protein concentration was quantitated using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). All lysates were diluted to a concentration of 1250 ng/μL ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (Invitrogen). Plasmids pGLDV_59-63 were created by replacing the sfGFP dropout part in the corresponding plasmids pGLDV_19 ...
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bioRxiv - Synthetic Biology 2024Quote: SKBR3-GFP cell line was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% FBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... barcoded sample was measured using Qubit™ Flex Fluorometer (Thermo Fisher Scientific), and the DNA fragment size of the amplicon was confirmed to have the expected size of 216 bp (ORI-marker ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1% penicillin-streptomycin (Gibco) and 50 μg/mL Normocin (Invivogen).
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bioRxiv - Synthetic Biology 2024Quote: ... Pierce iminobiotin agarose (Thermo Fisher Scientific) was equilibrated in falcon tubes and used to pack a PD-10 column up to a bed height of approximately 1 cm ...
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bioRxiv - Synthetic Biology 2024Quote: ... coupled to a quadrupole-orbitrap mass spectrometer (Q-Exactive, Thermo Fisher). The injection volume was 10 μL ...
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bioRxiv - Synthetic Biology 2024Quote: ... Data processing and ion annotation based on accurate mass were performed in Xcalibur 4.0 (Thermo Fisher) and Compound Discoverer 2.0 (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2024Quote: ... products were transformed into One Shot™ ccdB Survival™ 2 T1R Competent Cells (Invitrogen, A10460).
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bioRxiv - Synthetic Biology 2024Quote: ... T cells were activated using Human T-Activator CD3/CD28 Dynabeads (Thermo Fisher) at a 1:1 cell:bead ratio in T cell growth medium ...
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bioRxiv - Synthetic Biology 2024Quote: Ultraviolet photoelectron spectroscopy (UPS, ThermoFisher Nexsa) was used to detect the work function and valence band of CDs ...
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bioRxiv - Neuroscience 2024Quote: For transfection of HeLa and HEK293T cells Lipofectamine 2000 (# 11668030, Thermo Fisher Scientific) was used according to the manufacture’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... were diluted in 600 µl Opti-MEMTM (# 31985-062, Thermo Fisher Scientific) before adding 30 µl LipofectamineTM RNAiMAX and then for complex formation incubated for 15 min at RT.
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bioRxiv - Neuroscience 2024Quote: ... tissue was washed in DMEM with GlutaMAXTM-I and pelleted again (300 x g, 3 min, RT) before trituration in culture media (neurobasal media, # 21103-049, Thermo Fisher Scientific) with 100 U/ml penicillin ...
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bioRxiv - Neuroscience 2024Quote: ... the media was exchanged and after 36 h the media was replaced with 4 ml Opti-MEM® with GlutaMAXTM-I media (# 51985-026, Thermo Fisher Scientific). Media and cells were collected 48 h after transfection.
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bioRxiv - Neuroscience 2024Quote: ... and 1x B-27 supplement (# 17504-044, Thermo Fisher Scientific) with 25 U/ml benzonase and subsequent centrifugation (300 x g ...
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bioRxiv - Neuroscience 2024Quote: ... and coverslipped with Cytoseal mounting medium (Thermo Scientific, 8310). After immunohistochemical staining ...
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bioRxiv - Neuroscience 2024Quote: ... and 100 µg/ml streptomycin (# 15140148, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... The sections were then counterstained with Lerner 1 hematoxylin (Fisher Scientific, CS400-1D) and coverslipped with Cytoseal mounting medium (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA levels were normalized to mouse Gapdh or human RPL22 cDNA and relative transcription levels were analyzed using the comparative ΔΔCt method (7500 Software V2.0.5, Applied Biosystems, Life Technologies). The Lgmn mRNA level of the microglia-enriched fraction was obtained from a NanoString dataset (Omnibus GSE129709 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE129709)10.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for one hour in I-BlockTM (# T2015, Thermo Fisher Scientific). Proteins of interest were detected by the indicated primary antibodies followed by horseradish peroxidase-conjugated secondary antibodies detected by ECL Plus (# 32132×3 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins of interest were detected by the indicated primary antibodies followed by horseradish peroxidase-conjugated secondary antibodies detected by ECL Plus (# 32132×3, PierceTM ECL Plus Western Blotting Substrate, Thermo Fisher Scientific). For the quantitatively analysis ...
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bioRxiv - Neuroscience 2024Quote: ... and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAXTM-I (# 10566016, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... RT) and incubating it for 15 min at 37°C with 0.125% trypsin (# 25200-072, Thermo Fisher Scientific) and 25 U/ml benzonase (# E1014-25KU ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the soluble fraction was determined using the BCA protein assay (Pierce, Thermo Fisher Scientific) and equal amount of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-P ...
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bioRxiv - Neuroscience 2024Quote: ... and 5% normal goat serum (Invitrogen, 16210072), sections were incubated with all five clones ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using the BCA protein assay (Pierce, Thermo Scientific) and indicated amounts of protein in 50 µl LGMN-lysis buffer were pre-incubated in black 96-well plates (FluoroNunc ...
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bioRxiv - Neuroscience 2024Quote: ... HEPES (# 11594426, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... SuperBlock (# 37515, Thermo Fisher Scientific) was used to block the coverslips for 1-2 h at RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA concentrations were measured using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), DS-11 FX spectrophotometer (DeNovix ...
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bioRxiv - Synthetic Biology 2024Quote: ... Assemblies were then transformed into MegaX DH10β T1R Electrocomp™ Cells (Thermo Fisher Scientific, Waltham, MA), in 1 mm sterile cuvettes (VWR ...
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bioRxiv - Synthetic Biology 2024Quote: ... China) was equipped with a TG-WaxMS capillary column (60 m × 0.25 mm × 0.25 µm, Thermo scientific) and a flame ionization detector (FID) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL of 10X Buffer G (ThermoFisher), and nuclease-free water to make up to 10 μL ...