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Citations for Thermo Fisher :
2851 - 2900 of 3282 citations for CEPT1 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then transfected with the siRNA for knockdown or plasmid DNA for overexpression using lipofectamine 3000 (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: For the mini-screen the cells were plated in 96-well plates and transfected with 20 nM of pre-designed siRNAs (purchased from Horizon Discovery – Dharmacon, listed in Supplementary Table 4) with the use of Lipofectamine RNAiMAX (Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Wt MEF cells were transfected with the respective ON-TARGETplus siRNAs (Horizon; SCR control: D-001810-10; Bcl2L13: J-054573-08) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... then transfected with siRNAs of target genes or scramble controls for 3 days using Lipofectamine RNAiMAX (Invitrogen, cat# 13778-150). The culture medium was replaced with 150µl of differentiation medium DM-2 (Zenbio ...
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bioRxiv - Cancer Biology 2023Quote: ... 300,000 cells were transfected with 50nm ON-TARGETplus Human SMARCA4 siRNA (Dharmacon L-010431-00-0005) or the corresponding siControl (D0012061305) by lipofectamine RNAiMAX (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 1 × 105 MDM differentiated in MCSF for 4 days were transfected with 20 pmol of siRNA (LD013950D01, Dharmacon) using Lipofectamine RNAiMAX Transfection Reagent (Thermofisher). Transfection medium was replaced after 18 h with RPMI 1640 medium supplemented with 10% human serum and cells cultured for additional 3 days before pseudotyped virus infection.
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bioRxiv - Cell Biology 2023Quote: ... HeLa and U2OS cells were grown on 12 mm round coverslips (Marienfeld) and transfected with 10 nM of each siRNA using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: One day after seeding 106 U2OS cells in 6-well plate, small interfering RNAs (siRNA, 20μM) were delivered using Lipofectamine® RNAi-MAX Reagent (Invitrogen) according to the supplier’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Sac2 knockdown was performed with siRNA targeting mouse gene sequence Inpp5f: 5’-GGAAUGCGGUAUAAACGAATT-3’ and was from Ambion (Life Technologies). OSBP knockdown was performed using ON-TARGET plus SMART pool Mouse OSBP siRNA from Dharmacon ...
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bioRxiv - Microbiology 2023Quote: ... or the same concentration of scrambled siRNA (sense, 5’- UUCUCCGAACGUGUCACGUTT -3’; antisense, 5’- ACGUGACACGUUCGGAGAATT -3’) purchased from Tsingke Biotechnology (Beijing, China) with Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2023Quote: HT1080 cells were transfected with TRF2 siRNA oligonucleotides (synthesized from Eurogenetics Pvt. Limited)(Shalu Sharma et al., 2021) using lipofectamine 2000 (Invitrogen) transfection reagent according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were seeded in a 6-well plate overnight before being transfected with 2.5 μM of siRNAs using Lipofectamine RNAiMAX transfection reagent (Invitrogen, USA) according to the manufacturers instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 40 picomoles siRNA (2x 40 picomoles for dKD) and of 4μl Lipofectamine RNAiMAX (8μl for kD) (Invitrogen/Thermo Fischer Scientific) were added to each well ...
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bioRxiv - Microbiology 2024Quote: ... (Gene HMOX1, AM16708, Assay ID 11056, ThermoFischer) or with a non-targeting siRNA control (siCTRL, Dharmacon) using Lipofectamine RNAi MAX (Invitrogen) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: Pooled siRNA oligonucleotides (25pmol) targeting SMC5 (Horizon SMARTpool) were transfected into 1×106 cells using the RNAiMAX transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Briefly 40nl of Lipofectamine RNAiMax was added to 40nl 20 μM (stock concentration) siRNA in 10 μl Optimem (Gibco 31985062) and then added to cells ...
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bioRxiv - Neuroscience 2024Quote: ... L cells were (1 × 106) were transfected with 40 nM siGENOME SMARTpool siRNAs (Dharmacon) using LipofectamineTM RNAiMAX reagent (Invitrogen, 13778075) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: Cells were transfected with gene-specific siRNA oligos or a mixture of non-targeting control oligos using Lipofectamine RNAiMAX (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were plated at a confluence of approximately 40%-60% in basal culture medium and transfected with siRNA duplex using Lipofectamine TM 2000 reagent (ThermoFisher Scientific) according to the manufacturer’s instructions for 6 h ...
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bioRxiv - Microbiology 2020Quote: Cells were trypsinized to reverse transfect with 25nM of CTCF-specific or scrambled TARGETplus Smartpool siRNAs (Horizon, USA) using DharmaFECT4 (20% of amount recommended by the manufacturer’s protocol; Fisher Scientific, Dharmacon). Cells with no siRNA (un-treated ...
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bioRxiv - Cell Biology 2020Quote: ... 50 nM of the indicated siRNA pool and 6.5 μl of RNAiMAX reagent were added in 1 ml of OptiMEM (Thermo Fisher Scientific) to each well of a 6-well plate and incubated for 20 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Cells at 30% confluency were transfected with 20 nM final siRNA concentration using Lipofectamine RNAiMax transfection reagent (Life Technologies, 13,778–150) according to the manufacturer’s instructions and used for experiments after 48 hours for VPS35 or 72 hours for SNX4 ...
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bioRxiv - Cell Biology 2020Quote: ... pAVICs maintained at 60%– 70% confluency was transfected with 30nM USP9X or Scr siRNA using Lipofectamine 3000 Transfection Reagent (L3000015, ThermoFisher Scientific). Cell lysates were collected and examined for USP9X protein expression 72 hours post-transfection.
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bioRxiv - Immunology 2021Quote: ... siRNA was transfected using Dharmafect transfection reagent (Horizon Discovery T-2001-03) in a mixture of 200 µl OptiMEM (Gibco 31985), 3 µl Dharmafect and 0.03 nmol siRNA per 200,000 cells ...
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bioRxiv - Developmental Biology 2020Quote: ... 10,000 freshly sorted EpCAM+ DC were transfected with 10pmol of a pool of 4 ON-Targetplus siRNA (Dharmacon) (see Supplementary Table 5) for each candidate gene using Lipofectamine RNAimax (Life Technologies) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: TeloHAECs were transfected with a pool of Atp6ap2 (Dharmacon, L-013647-01-0005) and non-targeting control-siRNAs (Dharmacon, D-001810-10-05) using Lipofectamine 3000 (ThermoFisher, L3000015) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: All cells were transfected with 20 nM siRNAs or Antisense LNA GapmeR (Antisense oligo; ASO) using Lipofectamine RNAimax (Invitrogen™, 13778030) when seeded ...
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bioRxiv - Molecular Biology 2020Quote: ... myotubes were transfected with siRNA targeted against Phgdh using the liposome-mediated method (Lipofectamine RNAiMAX, Invitrogen, Cat# 13778100, Carlsbad, CA, USA). As a negative control ...
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bioRxiv - Cell Biology 2022Quote: ... Cells with 60~70% confluent were transfected with the indicated expression construct or siRNA using Lipofectamine™ 2000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... for NIH-3T3 cells and P19 cells or 5nM siRNA/8 µl INTERFERin (PolyPlus) for MDA-MB-231s and 200 µl Opti-MEM (ThermoFisher Scientific) in fresh media ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μg of plasmid and siRNA to a final concentration of 2.5 nM were added to 100 μl of OPTI-MEM Medium (Thermo Fisher Scientific). The mixture was incubated at room temperature for 30 minutes prior to the addition to the coverslips.
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bioRxiv - Cell Biology 2020Quote: ... 30 pmol of siRNA oligos was transfected onto the cells in each well with Lipofectamine RNAiMAX transfection reagents (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were transfected with siRNA oligonucleotides at the final indicated concentrations (Nup93 - 20nM, Nup188 - 20nM, Nup205 - 50nM, CTCF-50nM) using RNAiMax (Invitrogen, 13778) in reduced serum Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... Inc and utilized for transfection of HNSCC cell lines with a final concentration of 30 nmol/L siRNA with Lipofectamine RNAiMAX (Life Technologies). ROBO1 or EPHA2 expression vectors were constructed by fusing PCR fragments containing full length ROBO1 or EPHA2 upstream to eGFP ...
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bioRxiv - Neuroscience 2019Quote: ... and 6 μL of the mixture were added per well to the assay plates containing the siRNAs using an automated dispenser (Multidrop Combi, Thermo Fisher). Plates were briefly centrifuged and incubated at room temperature (RT ...
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bioRxiv - Immunology 2019Quote: DNA plasmids (Caspase-1-GFP and NOD1-mOrange) 59 or specific silencer and negative control siRNA (40 μmol of each; ThermoFisher Scientific) were diluted in Opti-MEM medium (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... of a pool of 2 siRNAs (Dharmacon ON□TARGET plus) directed against LUZP1 using the Lipofectamine® RNAiMAX transfection reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... of a pool of 2 siRNAs targeting either gene obtained from Dharmacon (ON□TARGET plus) using the Lipofectamine® RNAiMAX transfection reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The Quant-iT RiboGreen RNA Assay was used to determine siRNA encapsulation and concentration in LNPs according to manufacturer's protocols (ThermoFisher, Waltham, MA). LNP morphology was visualized by cryogenic transmission electron microscopy (cryo-TEM ...
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bioRxiv - Cell Biology 2019Quote: ... A gene-specific siRNA was added to transfect fibroblast cells for overnight using the RNAi Max reagent (Cat.13778150, Thermo Fisher). Cells then recovered in the complete medium for 24h ...
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bioRxiv - Biochemistry 2020Quote: ... we depleted endogenous PTP1B by transfecting the cells with 25 nM of a PTP1B siRNA silencer (AM16794, Thermo Fisher Scientific, Inc.), 12.5 μl Dharmafect ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transiently transfected with genes of interest or siRNA using a standard Ca2+ phosphate method as described previously63 or using manufacturers’ instructions for Lipofectamine 3000 (Thermofisher 18324010). Mnf2KO MEFs were obtained from the Laboratory of Prof ...
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bioRxiv - Microbiology 2021Quote: ... The pro-siRNAs were purified using Ni-NTA magnetic beads in a high-throughput manner using a KingFisher Flex Purification System (ThermoFisher Scientific). After another round of DEAE purification ...
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bioRxiv - Cancer Biology 2020Quote: ... After 24 h cells were transfected for 4 h at 37°C with 30 nM of siRNA using Lipofectamine 2000 in OPTI-MEM I according to the manufacturer’s instructions (Invitrogen, Paisley, UK).
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bioRxiv - Bioengineering 2020Quote: ... The efficiency of siRNA encapsulation N+:P− ratios was evaluated using a Quant-iT Ribogreen assay kit (ThermoFisher Scientific, Waltham, MA). siNP size was evaluated using dynamic light scattering (DLS ...
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bioRxiv - Biochemistry 2021Quote: Knockdown efficiency was assessed for siRNAs screened for use in this study by real time PCR using a StepOne Plus Real-Time PCR System (Applied Biosystems). Total cell RNA was harvested using Trizol™ (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... targeting the TLR3 gene (5’-CCUGAUGAUCUUCCCUCUAACAUAA-3’) and Stealth RNAi siRNA negative control med GC Duplex #2 were obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Neuro-2a cells seeded in 6-well plates (6×105/well) were transfected with 100 nM circTulp4-specific or negative-control siRNA by using Lipofectamine® RNAiMAX (Invitrogen). At 24 h post-transfection ...
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bioRxiv - Cell Biology 2021Quote: Human PCNT siRNA (Smart Pool) (M-012172-01-0005; Dharmacon) was transfected into cells with Lipofectamine RNAi MAX (13778100; ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Cell lines were transfected simultaneously with 400 ng TOPFlash firefly luciferase reporter and 100 ng Renilla luciferase constructs alongside either siRNA or expression constructs listed in Supplemental Table 2 using Lipofectamine 2000 (Life Technologies). Transfected cells were harvested after 72h and processed with Dual-Luciferase Reporter Assay kit (Promega) ...