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Citations for Thermo Fisher :
2651 - 2700 of 3282 citations for CEPT1 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Cells were transfected following the provider’s instructions for 32 hours (72 hours in case of Kif2a) with 20 nM siRNAs using Opti-MEM (Thermofisher, Switzerland) and Lipofectamine RNAiMAX (Thermofisher ...
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bioRxiv - Cancer Biology 2019Quote: Cells at 30–40% confluency were cultured in Opti-MEM Reduced Serum Medium and transfected with 50 nM siRNA duplexes using Oligofectamine (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and transiently transfected with either siRNA resistant pCMV(Δ5)-LEM2-mCherry (PL13) or siRNA resistant pCMV(Δ5)-LEM2Δ415-485-mCherry (PL17) using Lipofectamine LTX Plus (Thermo Fisher) for 24 h ...
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bioRxiv - Microbiology 2019Quote: Huh7 cells seeded at 5×105 in a 6 cm cell culture dish were transfected 24 hours later with the indicated siRNA duplex using Lipofectamine 3000 (Invitrogen) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and a negative control siRNA (catalog 4390843) were purchased and transfected into HepG2 cells using Lipofectamine® RNAiMAX (Life Technologies) or TransIT-siQuest® reagent (Mirus Bio ...
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bioRxiv - Physiology 2019Quote: ... MEFs were transfected with 30 nM control siRNA or a smartpool on-target plus siRNA for mouse CHOP (Dharmacon - L-062068- 00-0005) using Lipofectamine RNAi MAX (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... HepG2 cells were transfected with 25 nM SMARTpool siRNA for NCoR1 using Dharmafect 1 (Thermo Fisher Scientific, Waltham, MA, USA). ATG7-knockout HepG2 cells (Saito et al. ...
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bioRxiv - Genomics 2020Quote: ... 1×106 cells/well were seeded in 6-well plates prior to reverse transfection basically as before (O’Neill et al., 2018) using 100nM ON-TARGETplus SMARTpool siRNA (Suppl. Table 2) or scrambled control (all ThermoFisher Scientific). Posttransfection ...
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bioRxiv - Cancer Biology 2020Quote: siRNA sequences were either designed manually using commercially available soft wares or purchased as an already designed sequences (Horizon-ThermoFisher). The siRNA was re-suspended in 1X siRNA buffer (Dharmacon ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were transfected 24 hours after cell seeding with siRNA targeting Lamin A/C (siLMNA) or a control sequence (siCntl) using RNAiMax from Invitrogen. Adipogenic media and LIV treatment followed previously published protocol ...
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bioRxiv - Microbiology 2021Quote: ... and each well was transfected with 1.5 μL siRNA (20 nM) and 2 μL Lipofectamine 2000 (Thermo Fisher Scientific, USA), and Nc-siRNA was used as a transfection control ...
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bioRxiv - Cancer Biology 2021Quote: 70%-confluent cells were transfected using Lipofectamine RNAimax (siRNA) or Lipofectamine 2000 (plasmid) (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... A final concentration of 10-40 nM was used for the siRNAs and gapmer ASOs and PLUS Reagent (Life Technologies) added for transfections of gapmers.
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bioRxiv - Cancer Biology 2019Quote: ... human GRPR shRNA in Lentiviral Vector (Genomics Online; Limerick, PA, # ABIN3479889) and mouse Grpr siRNAs (Life Technologies, #157912 and #157914) were used for all knockdown experiments ...
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bioRxiv - Biochemistry 2020Quote: ... 4390771 ID: s69588 and s69586) or ERK3 siRNA (5 pmol; catalog number: 4390771 ID: s78451) and Lipofectamine 2000 (2 µl) (Invitrogen) and cells incubated for 19 h ...
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bioRxiv - Cell Biology 2021Quote: ... and then seeded at 500 cells/cm2 and transfected for 24 hours with 20 nM of siRNAs resuspended in Opti-MEM (Gibco), DharmaFECT siRNA transfection reagent (Fisher scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with 20 mM siGenome Smart Pool or On-Targetplus LAP1 siRNA oligonucleotides using optimem and lipofectamine RNAimax (Invitrogen). All siRNA oligonucleotides were from Dharmacon and are listed in Supplementary Table 16 ...
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bioRxiv - Cell Biology 2021Quote: ... For knockdown experiments MIN6 cells were resuspended with 25 nM antiGABA-B1 receptor or control siRNA (Dharmacon, siGENOME) pre-mixed with 1.6 μl/ml Lipofectamine RNAiMAX (Life technologies) in Opti-MEM I medium ...
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bioRxiv - Cell Biology 2021Quote: ... the Opti-MEM I medium was replaced by DMEM growth medium containing 25 nM siRNA and 1.6 μl/ml Lipofectamine 3000 (Life technologies) and incubated overnight.
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bioRxiv - Cell Biology 2021Quote: ... the transfect mixture (40 pmol of 3 independent siRNA targeting each gene/a non-targeting siRNA (negative control, NC) and 2 μl of Lipo 2000 which was diluted in 200 μl Opti-MEM medium (Gibco)) was added into each well and incubated for 6 hr at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 50 nM (Hec1) or 20 nM or 100 nM (Nuf2) siRNA for 48 h using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... a total of 100 nM siRNA oligos or inhibitor was transfected into the prepared protoplast of Foc using lipofectamine 2000 (Invitrogen). After two days culture ...
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bioRxiv - Neuroscience 2020Quote: ... HT22 cells were transfected with 15 nM cofilin1 siRNA for 48 h using Lipofectamine RNAiMAX (Thermo Fisher Scientific, Darmstadt, Germany) according to the manufacturer ...
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bioRxiv - Physiology 2021Quote: Silencing of the indicated genes was performed using forward transfection: 20 nM of the indicated SmartPool siRNAs listed in Dataset EV4 were mixed with Lipofectamine RNAiMax (Invitrogen), added on top of cells seeded in 6-well dishes (300,000 cells/well ...
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bioRxiv - Microbiology 2021Quote: ... siRNA reverse transfection was performed by mixing 1.2 μl siRNA 5 μM with 1 μl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 μl with Opti-MEM® (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5×105 HeLa were added to wells containing 15 pmol of siRNA mixed with 3 µL Lipofectamine RNAiMAX in 500 µL OptiMEM (Gibco) and incubated for 48 hours prior to further treatment or infection.
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bioRxiv - Cancer Biology 2020Quote: ... or 100 pmol (for each well in 6-well plate) of each siRNA was transfected into abl or DU145 cells using Lipofectamine RNAiMAX reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: The cells were transfected with K1 siRNA using Lipofectamine 3000 following the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). The sequences of the mouse keratin siRNA were CUCCCAUUUGGUUUGUAGCTT and UGACUGGUCACUCUUCAGCTT (GenePharma ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells in 6-well plates were transiently transfected with 4 µg of siRNA using Lipofectamine 2000 (Invitrogen). At 48h post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Ctrl or DDX5 siRNAs (40 nM each) were co-transfected with Renilla and Firefly luciferase vectors using RNAiMax (Life Technologies). Plasmids used are listed in Supporting Table S1 ...
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bioRxiv - Cancer Biology 2020Quote: ... The scramble control and PKD-1 siRNA (IDT) was then respectively transfected into the cells in Opti-MEM I reduced-serum medium (Gibco) by TransIT-X2 transfection reagent (Mirus ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: HUVECs were plated in 6-well tissue culture plates and transfected with siRNA at a 50 nM concentration with RNAiMAX (ThermoFisher) using the protocol provider by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... scrambled control and SMARTpool siRNA targeting p50 (Dharmacon, Lafayette, CO, USA) were delivered into H322M cells using Neon transfection system (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 75nM ON-TARGET plus siRNA SMARTpool against hC9ORF78 (020231-02) was transfected in 60% confluent 24 well plate using Lipofectamine 3000 (Invitrogen). Scrambled siRNA was used as a control ...
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bioRxiv - Cell Biology 2021Quote: ... 60 picomoles siRNA and 11 μl of RNAiMAX were diluted separately in 250 μl of Opti-MEM (Gibco#11058-021). These diluted solutions were mixed together ...
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bioRxiv - Cell Biology 2021Quote: ... or control siRNAs in suspension at a final concentration of 15 nM using Lipofectamine 2000 and Opti-MEM (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected 24 hours after cell seeding with siRNA targeting Sun1 and Sun2 (siSun) or a control sequence (siCntl) using RNAiMax from Invitrogen. Adipogenic media was placed on siRNA treated cells twenty four hours after the transfection ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were seeded in 13 mm coverslips (Agar Scientific) or in 10 cm dishes transfected with 200 nM siRNA using Oligofectamine (Invitrogen) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with the indicated siRNAs at a final concentration of 50 nM using Lipofectamine (13778075, Thermo Fisher Scientific) following standard protocols for cell lines ...
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bioRxiv - Cancer Biology 2022Quote: 500.000 MDA-MB-468 cells were transfected with 40 pmol siRNA upon seeding in 6 well-plates using Lipofectamine 3000 (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and transfected 2x at 0h and 24h with 20nM of siRNA (mixes or single) with GenMute transfection reagent (SignaGen) in cancer cell lines or Lipofectamine RNAiMAX (Invitrogen) in fibroblasts as in the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: H-1299 and H-23 cells were transfected with plasmids or co-transfected with plasmids and siRNAs using Lipofectamine 2000 (Invitrogen) as in the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... or non-targeting siRNA (50 nM) were transfected into HUVECs for 24 h and the proteasome inhibitor MG132 (0.2 µM, Fisher Scientific) was added to the HUVECs for 48 h ...
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bioRxiv - Biochemistry 2022Quote: ... or 14-3-3ζ in 3T3-L1 cells was achieved by transfecting preadipocytes one day prior to the induction of differentiation with 10 nM Silencer Select siRNAs (ThermoFisher) targeting Ctnnb1(s63417 ...
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bioRxiv - Cell Biology 2022Quote: ... IIA1.6 cells were transfected 60-70 h before live experiment with 40 pmol siRNA per 106 cells using the 10 μL Neon Transfection system (Thermofisher) and ON-TARGETplus Control n=Non-Targeting Pool (Dharmacon ...
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bioRxiv - Cancer Biology 2022Quote: The transfection of siRNA into MOLM-14 and MV4-11 cells was performed using the Neon transfection system (Life Technologies) according to the manufacturer’s recommendations (1700V ...
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bioRxiv - Cell Biology 2022Quote: ... SiRNA-mediated depletion of endogenous ESCO2 was done with 20 nM siRNA (Dharmacon, J-025788-09, target: CGAGUGAUCUAUAAGCCAA) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: hTERT RPE-1 cells were transfected with 20 nM (final concentration) of the respective siRNA for 48 h using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...