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Citations for Thermo Fisher :
2951 - 3000 of 3282 citations for CEPT1 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... or siRNA targeting ADK (GE Dharmacon L-062728-00-0005) were transfected at 12.5 nM using Lipofectamine RNAiMAX (ThermoFisher Scientific #13778150) according to manufacturer’s instructions.
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jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: ... as well as a non-targeting siRNA-control used for the specificity of the knockdown (Cat nb: 4390843) (ThermoFisher Scientific, USA). These two siRNAs (as well as the siRNA-control ...
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bioRxiv - Immunology 2020Quote: ... or equimolar mix of two human APOBEC3A siRNAs (Silencer 45715 and 45810, respectively, with sense sequences 5’-GACCUACCUGUGCUACGAATT-3’ and 5’-GCAGUAUGCUCCCGAUCAATT-3’, Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Percentage of EBOV-eGFP infected Huh7 cells (% infection) and cell count with different siRNA treatment were quantified by CellInsight™ CX5 High Content Screening (HCS) Platform (ThermoFisher) using a 10x objective.
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bioRxiv - Neuroscience 2021Quote: ... or with a pool of two different USP30 siRNAs (D1: 5’-CAAAUUACCTGCCGCACAA-3’; D3, 5’-ACAGGAUGCUCACGAAUUA-3’, Dharmacon; siUSP30) by using Lipofectamine RNAiMAX (Thermo Scientific) following the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... Encapsulation efficiency was calculated by determining the unencapsulated siRNA content by measuring the fluorescence intensity (Fi) upon the addition of RiboGreen (Molecular Probes) to the LNP and comparing this value to the total fluorescence intensity (Ft ...
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bioRxiv - Cancer Biology 2020Quote: Cells were reverse transfected with 40 nM SLC7A11, TP53, MYC, CDKN1A or non-targeting control siRNA pools (siGenome SMARTpool, Dharmacon) using Lipofectamine RNAiMAX solution (Life Technologies) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells were transiently transfected with the indicated siRNA at a final concentration of 8 nM using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer’s instructions ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... PC12 cells were plated in 6 well dishes and transfected 48h prior to lysis with 45 pmol siRNA using Lipofectamine RNAiMAX (Thermo Fisher); cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... Both Cstb and scrambled siRNA were labeled for the immunofluorescence assays using the Silencer Labeling Kit with FAM dye (Invitrogen, AM1634) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... HiPerFect transfection reagent was used to transfect cells with small interfering (si) RNA oligoribonucleotides targeted to rat ATF6 (assay ID# RSS315363, Stealth siRNA, Thermo Fisher). A non-targeting sequence (cat# 12935300 ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts were differentiated on cell culture plastic for 7 days before transfection with two separate siRNAs targeting either MMP8 (105332, 112911; Life Technologies) or MMP9 (104072 ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with 50 nM final siRNA for 72 h using Lipofectamine RNAiMax according to the manufacturer’s guidelines (Thermo Fisher Scientific). siRNA Oligonucleotides were synthesised by Merck (sequences are given in the Supplementary Table S1).
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bioRxiv - Genetics 2020Quote: THP-1 cells were plated at 0.6 × 106 cells/ml and transfected with 25 nM siRNA using Lipofectamine RNAimax transfection reagent (Invitrogen, catalogue 13788), according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Expression of RASSF1A was reduced by transfection of MCF with Small interfering RNA (SiRNA) SiRASSF1A (Thermo Fisher Scientific, catalog no. 185488) located on Chr.9 ...
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bioRxiv - Microbiology 2022Quote: ... or a nontargeting siRNA (12935300) at a density of 16,700 cells/cm2 using the Lipofectamine RNAiMAX transfection reagent (Life Technologies/Thermo Fisher) per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: AML12 cells were seeded at 1×105 cells/well in a 6-well plate and transfected with siRNA mixture (f.c. 10 nM) using Lipofectamine 2000 (Invitrogen, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 5×105 cells were seeded in 6 cm plates and transfected with siRNAs using Lipofectamine™ RNAiMAX Transfection Reagent (#13778030, Invitrogen) as per manufacturer protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... or non-silencing control siRNA (Dharmacon, SMARTpool) at a final concentration of 10 nmol/L using Lipofectamine RNA iMAX transfection reagent (Invitrogen 13778150) in antibiotic-free growth medium ...
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bioRxiv - Microbiology 2022Quote: A431D cells at a density of 2×105 cells/mL in serum and antibiotic-free media were transfected with a final concentration of 20 nM of the selected siRNA in a total reaction volume of 2 mL using lipofectamine RNAiMAX (Invitrogen; 13778075). Transfected cells were seeded onto a fibronectin-coated 12-well glass-bottom plate (Cellvis ...
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bioRxiv - Microbiology 2022Quote: Transient transfection of siRNA of a semi-confluent culture of KSHV-infected lymphatic ECFCs was done using 3 µl of Lipofectamine RNAiMAX (Invitrogen, Lithuania) and 150 pmol siRNA in a 6-well plate according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with ON-TARGETplus SMARTpool XIAP siRNA (3 μl) (Dharmacon, Lafayette, Co) using Lipofectamine 3000 (7.5 μl) (Invitrogen, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Clathrin and caveolin-1 knockdown in polarized hCMEC/D3 cell monolayers was performed using siRNA kit containing RNAi mix (Invitrogen, CA) and reduced serum medium ...
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bioRxiv - Cell Biology 2022Quote: Cells were transfected with 20 nM of each siRNA against LSM14A (Dharmacon) and PRMT1 (Dharmacon) using Lipofectamine RNAiMAX Reagent (Life Technologies) following the supplier’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Silencer Select siRNAs of Rheb (s12019, s12020, s12021), Rac1 (s11711, s11713), and Cdc42 (s2765, s2766, s2767) were purchased from Thermo Fisher. Dock7 shRNAs and negative control were purchased from Sigma (shRNA1 ...
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bioRxiv - Molecular Biology 2024Quote: Approximately 800,000 U2OS cells were reverse transfected with 20 nM siRNA against the 3’ UTR of endogenous TIRR or a negative control siRNA (Dharmacon ON-TARGETplus Human NUDT16L1 siRNA J-014872-23-0005 or ON-TARGETplus Non-targeting Control) using 3 uL of RNAiMAX lipofectamine (Thermo Fisher) in a 6-well plate ...
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bioRxiv - Molecular Biology 2024Quote: VSMCs were transfected with Small interfering RNA (siRNA) against Id2 (RiboBio, Guangzhou, China) using Lipofectamine RNAiMAX Reagent (Invitrogen, Carlsbad, CA, USA). The target sequence of mouse siRNA-ID2 was 5’-GACCCAGTATTCGGTTACT -3’ ...
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bioRxiv - Cancer Biology 2024Quote: ... #D-001810-10-05) or human GPX3 siRNA (Dharmacon, #L-006485-00-0005) were delivered to the cells using Lipofectamine RNAiMAX (Invitrogen, 13778150). After 24 hours ...
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bioRxiv - Cell Biology 2024Quote: Foreskin fibroblasts at 60-80% confluency 24 hours after seeding were treated with ON Targetplus SMARTpool siRNA oligos targeting FAM114A1 and FAM114A2 separately or simultaneously or were treated with a non-targeting negative control siRNA (Horizon Discovery) using Lipofectamine RNAiMAX transfection reagent according the manufacturer’s instructions (Thermo Fisher Scientific). Cells were treated with siRNA on day 1 and 3 after seeding ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse transfected with 1 pmol/well of a combination of 3 non-overlapping 11-mer siRNAs (Ambion Silencer® Select, Thermofisher) using Lipofectamine RNAiMax transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Cells were transfected with control and gene-specific siRNAs at a final concentration of 20 nM using the Neon transfection system 100 µl kit (ThermoFisher, MPK100) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Cells were treated with 40 nM non-targeting (NT1) or target-specific siRNA oligonucleotides (Dharmacon) using Lipofectamine RNAi-MAX (Invitrogen, 13778030) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... L-008436-00) or negative control siRNA (ON-TARGETplus Non-targeting Control Pool [Horizon Discovery], D-001810-10) using Lipofectamine RNAiMAX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... L-008436-00) or negative control siRNA (ON-TARGETplus Non-targeting Control Pool [Horizon Discovery], D-001810-10) using Lipofectamine RNAiMAX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with pre-incubated siRNA with a final concentration of 10 nM siRNA (3.75 μl of lipofectamine RNAiMAX in 125 μls of Opti-MEM, Gibco 31985-070) for 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with scrambled siRNA (Silencer Select negative control, Thermo Fisher 4390843 or Silencer Negative Control 1, Thermo Fisher AM4611) and APMAP siRNA (Silencer Select siRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were then transfected with 25 nM of either ON-TARGETplus Human ABCB1 (5243) SMARTpool or non-targeting pool siRNAs (negative control, Dharmacon) using the Lipofectamine RNAiMAX reagents (Thermo Fisher). Following 24 hours of incubation ...
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bioRxiv - Biochemistry 2024Quote: ... 000 cells were seeded onto a 6-well plate and reversely transfected with 25 nM of either non-targeting control siRNA (Horizon Discovery, D-001206-13-20) or SMARTpool siRNA targeting Znf706 (Horizon Discovery, M-021025-01-0005) using Lipofectamine RNAiMAX (Invitrogen, 13778150). The transfection media was exchanged after 24 hours ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: Cells were transfected for 2 days with siRNAs (synthesized by GenePharma) at 20 nM with the use of the Lipofectamine RNAiMAX reagent (Invitrogen, 13778150) and were then suspended ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were reversed transfected with 1 pmol (96-well) or 25 pmol (6-well) siRNA using Lipofectamine RNAiMAX (Life Technologies, 13778150) and Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Each siRNA construct was transfected at 220 pmol/l precomplexed and incubated in the transfection reagent in Opti-MEM (Gibco, ThermoFisher Scientific) for 4 h ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 10 nM of siRNA sequences (6-well plates) by using the Lipofectamine RNAiMax system (Thermo Fisher Scientific). Hypoxia was performed as previously described (24) ...
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bioRxiv - Cancer Biology 2024Quote: Parental cells were seeded at 5 × 105 cells per well into a 6-well plate and transfected after 24 hours with 20 μM siRNA (2 μl per well, 40 pmol final) using lipofectamine RNAiMAX (Invitrogen, 13778150) (5 μl per well ...
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bioRxiv - Neuroscience 2023Quote: ... cells were reverse-transfected with 20 nM of non-targeting (NT1) or target-specific siRNA oligonucleotides (Dharmacon On-Target Plus or siGenome, ThermoFisher Scientific), using Lipofectamine RNAi-MAX (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with siRNA oligos at a final concentration of 9 nM by forward lipofection using Lipofectamine 2000© (#11668019, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... oligos of small interfering RNA (siRNA) against MSC (si-MSC) and negative control (NC) were transfected using Lipofectamine 3000 reagent (Invitrogen, L3000015) at a final concentration of 37.5 nM ...
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bioRxiv - Microbiology 2022Quote: ... TZMbl or HEK293T cells were reverse transfected with 20 pmol siRNA per well of a 24 well plate using Lipofectamine RNAiMAX transfection reagent (ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... or non-targeting (Dharmacon) smartPool siRNAs at a final concentration of 80nM by using Lipofectamine RNAiMax transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... L-047399-01-0005) or Scramble (Dharmacon, ON-TARGETplus Non-targeting Control siRNA, D0018100205) at 200 nM final concentration using Lipofectamine 3000 (ThermoFisher, L3000001) in Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: MA104 cells seeded at a density of 5×105 in a 60 mm tissue culture plate were reverse transfected with 10 nM of indicated siRNA and 10 µl Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific) in a final volume of 5 ml as described above ...