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Citations for Thermo Fisher :
3051 - 3100 of 3282 citations for CEPT1 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 2×105 cells/well (6-well plate) HCECs were transfected with 30pmol siRNA using lipofectamine RNAiMax (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 200,000 cells were transfected in 12 well plate with 25 pmol siRNA (siTFEB : ON-TARGETplus Human TFEB, L-009798-00-0005, Dharmacon™) using Lipofectamine RNAiMAX Transfection Reagent (1:200; Life Technologies). Cells were incubated 72 h at 37°C prior to further manipulation or drug treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells and HepG2 cells were reverse-transfected with small interfering RNAs (siRNA) in a final concentration of 33 nM using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific). The siRNA targeted to human RHBDL4 (s38698 and s38699) ...
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bioRxiv - Cell Biology 2022Quote: ... 96-well plate) stably expressing GLuc-ASARTDL or GLuc-Untagged were reverse transfected with 10 nM siRNA using Lipofectamine RNAiMax (Thermo Fisher Scientific). A full media exchange was performed 48 h after transfection and after a further 24 h incubation cells were placed in hyperthermic or normothermic conditions for 24 h ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 3 different siRNAs for each PUM gene (their sequences are in Table S9) or control siRNA-A (sc-37007) at the final 40 nM concentration using the NEON transfection/electroporation system (Thermo Fisher Scientific). Transfections were performed in Buffer R using 10 μl NEON tips ...
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bioRxiv - Cancer Biology 2020Quote: ... HAP1 cells were seeded to a density of 5000 cells per well of a 96-well plate and 5nM siRNA was transfected with Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Medium (31985070 ...
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bioRxiv - Cell Biology 2019Quote: ... RNA interference was done by transfecting 100 nM siRNA (TPD54 1: GUCCUACCUGUUACGCAAU, TPD54 2:CUCACGUUUGUAGAUGAAA, TPD54 3:CAUGUUAGCCCAUCAGAAU) with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... transfected the next day with siRNA or LNA gapmers and fixed 48 hours post-transfection in ice-cold methanol (Acros Organics, 167830025) for 10 min at −20°C ...
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bioRxiv - Biochemistry 2020Quote: ... J774.1/JA-4 cells were seeded at 5 x 105 cells/well in 12-well plates and transfected with each siRNA (20 nM) using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Silencing RNA for NFIB and EZH2 (Silencer™ Pre-Designed siRNA) and scrambled control (Silencer™ Negative Control) were obtained from ThermoFisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... Cells in each setting were transfected with either 10nM scramble (control) or 10nM mouse SDHC siRNA-SMARTpool (Dharmacon, L048315-01-0005) using Lipofectamine 3000 (Life Technologies, L3000) and following the manufacturers recommended protocols ...
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bioRxiv - Cell Biology 2021Quote: ... Human onTARGETplus siRNA were diluted to 20 µM stock in RNase-free water and cells were transfected with 10 nM siRNA for 3 days with Lipofectamine RNAiMAX transfection reagent (Thermo Fisher, 13778075) in 6 well format ...
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bioRxiv - Plant Biology 2021Quote: ... 21 nt GAPDH-dsRNA (provided in the kit) was labeled with Cy™ 3 utilizing the Silencer™ siRNA Labeling kit (ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2021Quote: ... HAP1 cells were seeded to a density of 5000 cells per well of a 96-well plate and 5nM siRNA was transfected with Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Medium (31985070 ...
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bioRxiv - Molecular Biology 2020Quote: EndoC-βH1 cells were treated with DIMT1 siRNA and lysates were isolated using a proprietary lysis buffer (Pierce, Thermo Scientific, (Rockford, IL). Co-IP was performed at 4°C unless otherwise indicated ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transferred to medium containing CSS and transfected with 35-100 nM siRNA using the Lipofectamine RNAiMAX reagent (Invitrogen, Rockville IL) according to the manufacturer’s directions for a duration of 48-72 hours.
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bioRxiv - Cell Biology 2020Quote: Cells were either forward-or reverse-transfected with siRNAs using Lipofectamine RNAiMAX transfection reagent according to the manufacturer’s instructions (Thermo Fisher Scientific, 13778150). The concentration of single siRNA duplex used for transfection was 20 nM ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 × 106 CRC cells were transiently transfected with 400 pmol siRNAs via electroporation using the Neon Transfection System (Invitrogen, Carlsbad, CA, USA) with 3 pulses of 10 msec at 1,600 V according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... C2C12 cells (40% confluence) were transfected with siRNAs (100nM) using Invitrogen™ Lipofectamine™ RNAiMAX transfection reagent (ThermoFisher Scientific, Waltham, MA, USA) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... LS174T and LOVO cells were reverse transfected using 20 nM siRNAs and Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen™, Catalog no. 13778075) with slight modifications from the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: BJ cells were transfected with 60nM of human siRNA for different timings (indicated in the main text for each experiment) using Lipofectamine RNAiMax reagent (Invitrogen, 13778-075) and Opti-MEM medium (Gibco ...
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bioRxiv - Immunology 2020Quote: HBEC 5637 cells were grown to 60% confluency before transfection with either esr1-targeting siRNAs (ID-s4825, Cat. no. 4392420, Ambion, Waltham, MA) or non-specific (negative control or NC ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells in 150mM plates were transfected with 20 μL of siRNA (against DDX3 or a non-targeting control) using Lipofectamine 2000 (Thermo Fisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Downregulation of the 40 candidate proteins (Figure 1B and Figure 1 – figure supplement 1A) was conducted by using two different sets of pre-designed ON-TARGETplus SMARTpool custom siRNA libraries (Thermo Fisher Scientific). p97 was used as both positive and loading control ...
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bioRxiv - Neuroscience 2021Quote: ... 100 ng of the wt/mut-3’U R-pmirGLO plasmid and 1 pmol of PUM1 or NT siRNAs (Thermo Fisher Scientific) were co-transfected in quadruplicates for each condition using 1% Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM of siRNA non-targeting control (siRNA NTC, D-001810-10-05) or siRNA SOX11 (L-017377-01-0005, Dharmacon) were transiently transfected using DharmaFect 2 (Thermo Fisher Scientific) according to the manufacturer's guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... as directed by the manufacturer with 10 pmol of siRNA (Control or On Target plus Smart Pool mouse FMR1; Thermo Fisher Scientific) in a final volume of 600 μL ...
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bioRxiv - Cancer Biology 2021Quote: ... Silencer™ Select)and FAM134B (siRNA ID: s29012, s29013; Silencer™ Select) together with the transfection reagent Lipofectamine 3000 (L3000-008; Invitrogen) for 48 h according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Transient transfections of SMARTPool SGK3 siRNA (L-004162-00-0005, Horizon Discovery) were performed with Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... 3 μl of 10 μM stock siRNA was diluted with 100 μl of Opti-MEM® Reduced-Serum Medium (Thermo Fisher Scientific) and mixed with 1.5 μl of Lipofectamine® RNAiMAX Transfection Reagent (Thermo Fisher Scientific) ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 293T cells were seeded one day prior to transfection with clathrin siRNA (Horizon Discovery, Dharmacon™) using Invitrogen Lipofectamine RNAiMax (ThermoFisher Scientific). One day later ...
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bioRxiv - Genetics 2022Quote: ... HEK293FT cells were seeded in 12-well plates at 20% confluence and the following day transfected with 20 nM of the siRNA pool using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Hek293-Klotho cells were seeded at density of 200 000 cells/well on 12-well tissue culture plates and transfected with ARHGDIA Ambion™ Silencer™ Select Pre-Designed siRNA (4427038 Ambion, distributed through Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Cells were transfected with the indicated siRNAs (15 pmol of total siRNA per 35-mm dish) (Baggs et al., 2009) using the Lipofectamine 3000 transfection reagent (Thermo Fisher # L3000) and according to the manufacturer’s instructions for 6-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... myotubes (differentiated for either 2 or 4 days) were transfected twice for 48 h using 200 nM siRNA and Lipofectamine RNAiMax transfection reagent (Life Technologies, France) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... H9c2 cells or CFs were transfected with 1µg/ml of MYOCD siRNA (Table S1 in supplementary data) which targets both the cardiac isoforms (Silencer® Select pre-designed MYOCD siRNA; ID s140959; Ambion, USA). Cells transfected using scrambled sequence anti-miR (Ambion ...
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... cells were transfected with ON-TARGETplus SMARTpool siRNA duplexes (Dharmacon, Lafayette, CO, US) using Lipofectamine RNAiMAX reagent according to the manufacturer’s instructions (Invitrogen, Waltham, MA, US). For WRN knock-down ...
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bioRxiv - Microbiology 2019Quote: ... 7,500 cells per well) were reverse transfected with 10 nM siRNA (each condition in quadruplo) using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific). One day later ...
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bioRxiv - Cancer Biology 2019Quote: ... HT-144 cells were transfected with siRNAs at a final concentration of 50 nM using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) at a density of 2 × 105 cells/well in a 6-well plate ...
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bioRxiv - Immunology 2019Quote: Ptpn2 was knocked down transiently in HER-2 CAR T cells using nuclease resistant Ptpn2 siRNA duplexes (Ptpn2; GCCCAUAUGAUCACAGUCG) (siSTABLE™; Dharmacon Thermo Scientific); siRNA duplexes (siSTABLE™ ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transiently transfected for 48 hours with control and siRNA targeting XBP1s (Eurofins Genomics, Les Ulis, France, 5’ AGAAGGCUCGAAUGAGUG 3’) using Lipofectamine iRNA max (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... siRNA libraries against 6,881 gene targets were prepared in the 96-well format at the concentration of 3 µM (diluted in Thermo Scientific siRNA buffer) at the Division of Infection and Pathway Medicine ...
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bioRxiv - Microbiology 2019Quote: ... 96-well plates in triplicate and transfected with siRNA oligonucleotides 24 h later by using the RNAiMAX transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells stably expressing ss-HRP were transfected in 96-well plate pre-arrayed with the indicated smart pools siRNA (Dharmacon) using Lipofectamine®2000 transfection reagent (ThermoFisher, #12566014). Briefly ...
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bioRxiv - Biochemistry 2019Quote: We knocked down endogenous A3B in Hs578T cells using 10 nM siRNA (Dharmacon, J-017322-08-0005) and Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were transfected with 2.5 µM ON-TARGET plus human SAMHD1 siRNA SMART-pool obtained from Dharmacon (Munich, Germany, L-013950-01-0050) in resuspension electroporation buffer R (Invitrogen, Dreieich, Germany) using the Neon transfection system (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse secondary antibody Alexa 488 labeled (cat no: A11008; used at 1:500 dilution for immunocytochemistry) and α-SNAP (NAPA) siRNA were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2021Quote: ... cells were seeded in 6-well plates or 6 cm dishes and reverse transfected with 5 nM Silencer Select siRNAs (Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... with transfection reagents containing the siRNAs (final concentration: 6 nM in culture medium) and Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific; #13778030) in Opti-MEMI following the manufacturer’s instructions ...