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Citations for Thermo Fisher :
2701 - 2750 of 3282 citations for CEPT1 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: HeLa mito-mKeima cells were seeded at 200,000/well in a 6-well plate 12-16h prior to transient transfection with the indicated siRNAs (20 nM) with Lipofectamine RNAiMAX (ThermoFisher). The liposome/siRNA mixture was added to culture media for 24h ...
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bioRxiv - Cell Biology 2022Quote: ... each siRNA was transfected at a concentration of 220 pmol/l with transfection reagent in Opti-MEM Medium (Life Technologies). Cells were incubated with the siRNA complexes for 4 h ...
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bioRxiv - Cell Biology 2022Quote: ... Hela cells were plated on cover slips and then transfected with a total of 600 pg of either a scrambled control siRNA oligo medium GC content (Invitrogen) or a mix of three siRNA oligos HSS121071 (Epsin-1) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected 24 h after seeding with 50-100 nM SMARTpool siRNAs (Dharmacon) for gene silencing and Lipofectamine 2000 (1000186, Invitrogen), with two rounds of knockdown for 5 days ...
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bioRxiv - Cell Biology 2022Quote: ... The siRNAs used to test the role of spatacsin interactors identified by the two-hybrid screen were Silencer Select siRNAs from ThermoFisher and are listed in Supplementary Table 3.
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bioRxiv - Microbiology 2020Quote: For siRNA transfection 50 000 cells were plated in a 24-well plate and immediately mixed with Lipofectamine RNAiMAX (Invitrogen) following the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2020Quote: siRNAs targeting HLX (catalog no. 4427037, ID: s6639) and MYC (catalog no. 4427037, ID: s9129) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... or a 96-well plate (viability) and then were reverse transfected in duplicate with 30 nM of siRNA using the Lipofectamine RNAiMAX reagent (Invitrogen) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... cells reached 90-95% confluency and were transfected with siRNA twice (4 hours apart between first and second transfections) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... the cells in each well were transfected with 20 pmol siRNA targeting to human XPO1 (GenePharma, Shanghai, China) using lipofectamine 2000 (Invitrogen). At 6 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were re-transfected with 52 pmol of siRNA and 800 ng of the indicated plasmids using Lipofectamine 2000 (Invitrogen). Protein and total RNA were isolated after two additional days ...
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bioRxiv - Molecular Biology 2020Quote: ... Two days later cells were co-transfected with a plasmid expressing I-SceI (pCBA-I-SceI) together with indicated siRNAs using Lipofectamine 2000 (Invitrogen). Cells were harvested after three days post transfection and subjected to flow cytometric analysis to identify and quantify GFP-positive cells (BD FACS Calibur ...
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bioRxiv - Immunology 2020Quote: ... M-014142-00-0005) or non-targeting siRNA Pool #1 (Dharmacon, Horizon Discovery, D-001206-13-05) were transfected using Lipofectamine RNAiMax (Invitrogen), and experiments were performed at 48 hours post-transfection.
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bioRxiv - Cell Biology 2020Quote: ... siRNA transfections were carried out in 60 mm tissue culture plates using the Lipofectamine RNAiMax transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Control cells were transfected with 50nM scrambled siRNA (ON-TARGETplus, non-targeting pool, Dharmacon) using Lipofectamine RNAiMax (Thermo Fisher scientific). Cells were analysed 48 hr post-transfection for all assays ...
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bioRxiv - Immunology 2020Quote: ... A549 and BEAS-2B cells (at approximately 50% confluence) were transiently transfected with 40 pM si-control and siRNAs for other genes using Lipofectamine 2000 (Invitrogen) according to manufacturer instruction ...
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bioRxiv - Biochemistry 2021Quote: ... Media was changed to fresh antibiotic-free media just prior to transfection with 20 picomoles siRNA and Lipofectamine RNAiMAX transfection reagent (Invitrogen). 48 hours after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 μg of pcDNA3.1+ empty vector were transfected into cells at least 6 hours post-siRNA transfection using lipofectamine LTX with Plus reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... ARPE-19 cells were transfected by reverse transfection in 24-well tissue culture plates as follows: A total of 6 pmols of siRNA was diluted in 100 μl of OptiMem (Gibco) per well ...
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bioRxiv - Microbiology 2021Quote: Huh-7 cells seeded in 6 well plates were transfected with human WWOX-specific or random siRNAs (DHARMACON) at a final concentration of 50nM per well using Lipofectamine 2000 reagent (INVITROGEN). At 24 hours post siRNA transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 20 nM pooled control or P2Y2-targeting siRNAs from a siGENOME SMARTpool (Dharmacon, GE Heathcare) with Lipofectamine 3000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... siRNA reverse transfection was performed by mixing 1.2 µl siRNA 5 µM with 1 µl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 µl with Opti-MEM® (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 pmol of siRNA was mixed with 4 µl of Lipofectamine™ RNAiMAX Transfection Reagent (Cat. No. 13778030, Thermofisher scientific) in Opti-MEM™ I Reduced Serum Medium and incubated for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then transfected with 10nM final concentration of control or target siRNAs using Lipofectamine™ RNAiMAX (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... hiPSC-CMs were then transfected with siRNAs directed against PAX9 and EGR2 (ON-TARGETplus Human) using lipofectamine RNAi Max (ThermoFisher). Each siRNA was tested individually in 8-plicates ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded in six-well plates at 30% confluence and transfected with 12.5-25 nM final siRNA concentration using Lipofectamine RNAiMax (Life Technologies) according to the manufacturers’ instructions and cells were then used for experiments 48 or 72 h after knockdown ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with scrambled small interfering RNA or small interfering RNA (siRNA) to silence TMX4 expression (5′-GCAUGGUGUUCUUACGUUUtt-3′, 100 nM per dish, Silencer Select Pre-designed, Ambion). Cells were processed for immunofluorescence or for biochemical analyses after 48h of transfection.
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bioRxiv - Microbiology 2019Quote: ... The complete protocol can be found in Virus Host Interactions Methods and Protocols (30). Low passage NHDF cell suspension (approx. passage 11) were reverse-transfected with siRNA and Lipofectamine RNAiMAX (Invitrogen) using MultiDrop 384 ...
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bioRxiv - Cell Biology 2019Quote: ... 3000 HeLa-ss-HRP cells in 80 μl of complete medium lacking penicillin/streptomycin were plated into 96-well plate containing 20 μl of 250 nM siRNA and 0.5% (v/v) lipofectamine 2000 in OptiMEM (ThermoFisher, #319885062). Medium was replaced after 24h ...
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bioRxiv - Cell Biology 2019Quote: RNAi in HeLa cells was performed in a 24-well format with 2.5 pmol of small interfering RNAs final concentration (siRNAs) using Lipofectamine® RNAiMAX Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: STHdhQ111/Q7 cells were reverse transfected with pooled small interfering RNAs (siRNAs) using Lipofectamine 2000 (Life Technologies, cat. no. 11668). Cells were treated with a pool of four small siRNAs per gene with the following sequences (Qiagen 1027280):
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bioRxiv - Developmental Biology 2020Quote: ... Cells were transfected with 24 – 72 nM siRNA in transfection medium supplemented with 2% charcoal-stripped FBS (catalog no. 12676-029, Gibco) for 24-48 hours before replacing with fresh growth medium ...
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bioRxiv - Immunology 2020Quote: Stimulated T cells were transfected with 150 pmol siRNA at 1600 V for 10 ms with three pulses using the NEON transfection system (ThermoFisher). 24 hours later ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were transfected with siRNA at a final concentration of 20 nM in 384-well plates using 0.1 µL RNAiMAX (Life Technologies) per well in 100 µL cell culture medium ...
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bioRxiv - Cell Biology 2020Quote: Cells were transfected with 40-50 nM of siRNAs at the time of plating and again 24 h later with either Lipofecatamine RNAiMax or Lipofectamine 2000 (Invitrogen) using manufacturer’s protocols.
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bioRxiv - Cell Biology 2021Quote: ... expressing CD4-SIV Env chimeras were seeded and transfected the same day with siRNA oligonucleotides (145 nM) using Oligofectamine (Invitrogen). Oligonucleotides targeting the μ2 subunit of adaptor protein complex 2 (AP2 ...
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bioRxiv - Neuroscience 2019Quote: ... small interfering RNA (siRNA) oligos against JPH4 were transfected into the DRG with Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific). DRG neurons were dissociated as previously described ...
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bioRxiv - Microbiology 2020Quote: ... 2×104 MDCK cells suspended in serum free media were reverse-transfected with siRNAs at 20 nM final concentration using 0.25 µL lipofectamine RNAiMAX (Invitrogen 13778075). The transfection mix was replaced by full media 8 h later ...
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bioRxiv - Biochemistry 2019Quote: Undifferentiated pre-adipocytes were transfected with scramble RNA or MPC1 siRNA using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Roskilde, Denmark) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Clonal selected U2OS cells stably expressing MYCT58A under the control of the retroviral LTR were transfected with 50nM siRNA in a 96-well format using Dharmafect1 (Dharmacon) or Lipofectamine RNAiMAX (Invitrogen). 24 hours after transfection ...
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bioRxiv - Biochemistry 2019Quote: ... knock-down was performed using a single HELLS siRNA oligonucleotide 24h prior to transfection of pMyc-HELLS plasmid DNA using Lipofectamine 3000 (Invitrogen). Media was changed 6 h after plasmid transfection and cells incubated for 24h prior to irradiation ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA-seq libraries for the STAT3 siRNA and Dexamethasone treatment were prepared from 250 ng total RNA via polyA-selection (Dynabead mRNA Purification Kit, Invitrogen) followed by transposase-mediated non-stranded library construction(73) ...
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bioRxiv - Genetics 2020Quote: ... we reverse transfected approximately 3.0 x 105 - 5.0 x 105 293T/TLR cells with 75 nM of siRNA per well of a 6-well plate using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA transfections for FLAG-ASK3-HEK293A cells were carried out by forward transfection using Lipofectamine RNAiMAX (Invitrogen, Cat. #133778-500) and 10 nM siRNAs once the cells reached 40–80% confluency ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were reverse-transfected with 20nM of siRNA using Lipofectamine™ RNAiMAX Transfection Reagent (Thermo Fisher Scientific, cat. no. 13778150) diluted in Opti-MEM™ I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... medium was exchanged to DMEM with 10% PDS and cells were transfected with 40 nM siRNA using lipofectamine 3000 (Invitrogen). On day six ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 nM siRNA was incubated in a cell culture plate for 20 min with RNAimax in OptiMEM medium (Life Technologies) onto which the cells were seeded ...
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bioRxiv - Biophysics 2020Quote: ... The bromelain treated KG1a cells were then transfected using 250 pmol of CD34 siRNA (Silencer Select, Life technologies; 4392420-s2644) or 250 pmol of a scrambled control siRNA (Silencer Select ...
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bioRxiv - Cell Biology 2021Quote: Silencing of the indicated genes was performed using forward transfection: 20nM of the specific siRNA was mixed with Lipofectamine RNAiMax (Invitrogen), added on top of seeded cells and left at 37°C in a CO2 incubator for 72h ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated on collagen-coated plastic (50µg/mL) and transfected 24h later at 70-80% confluence using siRNA (25nM) and Lipofectamine 2000 reagent (Life Technologies), according to the manufacturers’ instructions ...