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Citations for Thermo Fisher :
3101 - 3150 of 3282 citations for CEPT1 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... cells were transfected with 50 – 100 nM siRNA and Atufect transfection reagent (Silence Therapeutics; Jurkat and HeLa) or Lipofectamine RNAiMAX reagent (Thermo Scientific; U2OS) and analyzed after 72 h ...
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bioRxiv - Cancer Biology 2020Quote: ... L-013571-00-0005), or siERBB2 (Dharmacon, L-003126-00-0005) SMARTpool siRNA was transfected per well using Lipofectamine™ RNAiMAX (Thermofisher, 13778150) as per the manufacturer’s instructions and incubated for 72 hours ...
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bioRxiv - Cell Biology 2021Quote: ... by diluting stock siRNA solutions to a final concentration of 50 nM and 6.3 μL Lipofectamine RNAiMAX transfection reagent (ThermoFisher Cat. No. 13778030) per well of a 6-well tissue culture dish ...
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bioRxiv - Cancer Biology 2020Quote: ... along with 20 nM siRNA (unless dose is otherwise indicated) in a 1:2 ratio with Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) for 16-24 hours at 37°C in 5% CO2.
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bioRxiv - Developmental Biology 2021Quote: ... whereas the contralateral right eye of the same animal was injected with an equal amount of negative control scrambled siRNA (si-Ctrl) (ThermoFisher, Cat# 4390844). After injection ...
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bioRxiv - Cancer Biology 2021Quote: PCa cells were transfected with siRNAs (at a final concentration of 10 nM) using Lipofectamine RNAiMAX Transfection Reagent (#13778150, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cells were typically transfected in a 6 well plate format with 20 μM siRNAs and 4 μL Lipofectamine RNAiMAX (Thermo Fisher Scientific); the depletion phenotype was inspected 72 h after transfection unless otherwise indicated in the text or the legends ...
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bioRxiv - Cancer Biology 2021Quote: ... U2OS reporter cell lines (kind gift from Prof J Stark, USA (27)) were transfected with siRNAs (40nM) using lipofectamine RNAiMAX (Thermo Fisher Scientific) and transferred into new plates on day two ...
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bioRxiv - Immunology 2020Quote: ... 5 million cells were washed twice with PBS and the cell pellet was nucleofected with 10nM Silencer Select STAT3 s745 (sequence for sense GCACCUUCCUGCUAAGAUUTT and antisense AAUCUUAGCAGGAAGGUGCCT) or Silencer Select Negative Control (sequence proprietary) siRNA (Thermo Fisher Scientific) using the Amaxa Human T Cell Nucleofector Kit (Lonza ...
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bioRxiv - Molecular Biology 2020Quote: ... approximately 400.000 cells were plated in a 6-well dish and reverse transfected with a final siRNA concentration of 50 nM using Lipofectamine RNAiMax (Thermo Fisher Scientific). 48 hrs post transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... cells (GM10253A and HybNeo3; 10%–20% confluent) were transfected with 10 nM Silencer Select Pre-designed siRNA targeting EXOSC3 (Ambion, Life Technologies) using Lipofectamine RNAi MAX (ThermoFisher ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: We transfected cells with siRNAs and/or Antisense LNA GapmeRs (Antisense oligo; ASOs) for a 20 nM final concentration using Lipofectamine RNAimax (Invitrogen™, 13778030) when seeded ...
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bioRxiv - Cell Biology 2021Quote: ... with different combinations of CRTC1 siRNA and/or miR-184 inhibitor: 100 nM negative control miRNA inhibitor (cat. 4464076-Invitrogen, Pittsburgh, US) + 50 nM of CTR siRNA (cat ...
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bioRxiv - Immunology 2022Quote: ... AC16 were stably transduced with lentivirus pISRE-sfGFP and pools were treated with 100IU/ml of recombinant IFNβ or transfected with non-targeting control or MEF2A-targeting siRNA in the presence of the indicated inhibitors prior to epifluorescent imaging using an EVOS cell imaging system (Thermo Fisher Scientific). For flow cytometric assessment of fluorescence expression ...
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bioRxiv - Microbiology 2022Quote: ... or a nontargeting siRNA (12935300) at a density of 16,700 cells/cm2 using the Lipofectamine RNAiMAX transfection reagent (Life Technologies/Thermo Fisher) per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... MDA-MB-231 cells were seeded at a density of 500,000 cells/well in a 6-well plate and transfected with siRNA targeting EED using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) for 2 days ...
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bioRxiv - Cell Biology 2022Quote: Cells were treated with 100 µM of ON-TARGETplus Mouse Rab27a (11891) or Rab27b (80718) siRNA SMARTpools (Dharmacon, Lafayette, CO, USA) complexed with Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Transient transfections with plasmids and small interfering siRNA were done using Lipofectamine 2000 reagent (Catalog number 11668027, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instruction.
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bioRxiv - Developmental Biology 2022Quote: ... RNAi Inc.) or 4 μM red fluorescent non-targeting siRNA (BLOCK-iT™ Alexa Fluor® Red Fluorescent Control; Thermo Fisher Scientific) was prepared as siRNA solution ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Microbiology 2022Quote: ... or 60 pmol of siRNA for c-Jun (Dharmacon ID L-003268-00-0010) was diluted in Opti-MEM (Invitrogen, Carlsbad, CA). Separately ...
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bioRxiv - Molecular Biology 2023Quote: ... or a commercially available on-target siRNA against mouse Ep400 (Dharmacon J-058750-12) (siEP400) using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher 13778075). Cells were maintained for 72 h before harvest.
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bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Molecular Biology 2023Quote: ... they were transfected at 40% confluency with 200 ng of the pcDNA-MATR3 vectors and 10 μM of either control or MATR3 siRNA per well using the Lipofectamine 3000 transfection reagent (ThermoFisher Scientific L3000015). ON-TARGETplus non-targeting siRNA #1 ((Dharmacon D- 001810-01-05) ...
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bioRxiv - Physiology 2024Quote: ... R-5’-3’ UGGUUCAUCAGCCUUUAACTT) and mock siRNA (General Biosystems, China) were transfected into mouse DCT cells by Lipofectamine™ 3000 (Invitrogen, L3000015) (20 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... We used siDESIGN Center (Horizon Discovery) to design siRNA (siUNG#4) targeting the 3′UTR region of UNG mRNA (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic®) and subcloned into pENTR (Life technologies®). GST- ...
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bioRxiv - Genomics 2024Quote: ... in U251 and U343 cells was performed by reverse transfection with 25 nM of control or SERBP1 SMARTpool siRNA (Dharmacon, Cat# L-020528-01-0005) using Lipofectamine RNAiMAX (Invitrogen, Cat# 13778150).
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bioRxiv - Cell Biology 2024Quote: ... Transfection was performed by combining 40 nM of either ALIX-specific or control siRNA (Table 1) and 7.5 µl Lipofectamine® 3000 (Thermo Fisher Scientific) in 250 µl serum-deprived DMEM/F12 medium and incubated for 15 min at room temperature prior to dropwise addition onto the cells ...
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bioRxiv - Cell Biology 2023Quote: ... 100 nM of each siRNA was transfected into 105 HeLa cells using Lipofectamine RNAiMAX (#13778075, Invitrogen, Carlsbad, CA, USA; Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... SH-SY5Y and SK-N-BE(2)-C neuroblastoma cells were transfected using the Neon transfection system (MPK10096) with two siRNAs toward PHF6: siPHF6-48 (s38848, Life technologies, #439242) and siPHF6-SP (SMARTPool 84295 ...
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bioRxiv - Immunology 2024Quote: BMMCs were transfected with siRNA cocktails targeting Zc3h12a and/or Zc3h12c (Horizon Discovery/Dharmacon) using the 100 µl Neon Transfection System Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with siRNA targeting the gene of interest or a scrambled control using Lipofectamine RNAiMAX according to manufacturers’ instructions (Life Technologies, 13778030). ON-TARGETplus SMARTpool siRNAs were purchased as custom cherry-pick libraries from Dharmacon and used at a final concentration of 10 nM ...
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bioRxiv - Cell Biology 2024Quote: Knockdown of RORα in human cells was performed using the siGenome SMARTpool Human RORA (#6095) siRNA from the Horizon Discovery “Dharmacon” portfolio (#M-003440-01-005) and Lipofectamine 2000 (Invitrogen, #11668-030). The siRNA stock was diluted to 20 µM in RNase-free water following the product protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Each siRNA construct was transfected at 220 pmol/l precomplexed and incubated in the transfection reagent in Opti-MEM (Gibco, ThermoFisher Scientific) for 4 h ...
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bioRxiv - Immunology 2023Quote: PPQ cells grown in 6-well plates were transfected with 100 nM control or cGAS siRNA (Dharmacon) by RNAiMax (Thermo Fisher Scientific). cGAS knockdown was confirmed by western blot three days later ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T/17 cells (ATCC, CRL-11268) grown and passaged in DMEM (5% FBS) were transfected by siRNA using RNAiMax™ (Invitrogen, 13778150) in Optimem (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 1×105 cells were seeded in 6-well plates overnight and transfected with 20-25 pmol of the indicated siRNAs using Lipofectamine 3000 (ThermoFisher Scientific, 13778030) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Reverse transfection in HEK 293 cells was performed with 3 nM final siRNA concentration with Lipofectamine™ RNAiMAX (ThermoFisher, Cat. No. 13778075) according to manufactural procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... As previously mentioned the LOX or LOXL1 siRNA or siCTRL were complexed with the transfection reagent Lipofectamine RNAiMax (Thermo Fisher Scientific, 13778075) and added to the NHLF cells ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 30 pmol of target gene or control siRNA directly after replating on the 6 cm culture plate using Lipofectamine® RNAiMAX (#13778-075, Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Knockdown of PR130 in murine and human PDAC cells was performed by transfecting 30 pmol of validated siRNA molecules targeting the PPP2R3A/Ppp2r3a mRNAs (murine: Santa Cruz, sc-108917, 10 µM stock solution; human: Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Cells were transfected with non-silencing siRNA or siFOXM1 (Horizon Discovery, #001810-10-05, #057933-01-0005 & 009762-00-0005) using Lipofectomine RNAiMAX (Thermo Fisher, #13778150). Lentiviral cloning vector pLKO.1-TRC (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: ... 5×105 cells were transfected with 15nM siRNA pools or DNA (Supplementary Table TS1) using Lipofectamine RNAiMax or Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... D-001810-10-05) or Zmiz1 siRNA (Horizon, L-007034-00-0005) for 48 hours hours using Lipofectamine 3000 (Thermo Fisher, L3000015). The final concentration of siRNA solution was 200 nM ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA transfection was carried out at a concentration of 60 pmol in Opti-MEM medium using Lipofectamine RNAi-MAX (Invitrogen Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 ml of Hela cells were seeded in 6-well plates at 37,000 cells/ml and transfected using 10nm of siRNA and 1.5 μl of the Lipofectamine™ RNAiMAX transfection Reagent (Thermo Fisher Scientific) per well ...
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bioRxiv - Cancer Biology 2023Quote: ... SMARTpool ZMIZ1 (L-007034-00)and control siRNA (D-001810-10, Dharmacon) were transfected with Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... H295R cells were seeded in 6-wells plate at a density of 0.3×106 cells/well and transfected the day after with a CTNNB1 siRNA (Thermofisher, Carlsbad, USA, #s436) or a control siRNA (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... ON-TARGETplus Mouse Dlg1 SiRNAs (SMARTpool, L-042037-00-0005 were used for Unc5B gene deletion. Transfection was performed using lipofectamine RNAi max (Invitrogen, 13778-075) according to the manufacturer’s instruction with siRNA at a final concentration of 25pmol in OptiMem for 8h ...