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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... in electroporation buffer R (ThermoFisher) with 2% glycerol62 ...
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bioRxiv - Biophysics 2024Quote: ... Beads had previously been conjugated with 5 μL anti-GFP antibody (Invitrogen, A6455) for 2 hours at RT ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific™, Cat# 78501) supplemented with Protease Inhibitor Cocktail and Halt Phosphatase Cocktail (Thermo Scientific™ ...
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bioRxiv - Biophysics 2024Quote: ... using PowerUp SYBR green master mix (Thermo Fisher, A25778). All quantifications of mRNA levels were first normalized to an endogenous housekeeping control (Tbp) ...
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bioRxiv - Cancer Biology 2024Quote: ... Tris-buffered saline (TBS: 20 mM Tris-HCl, pH 7.5, containing 0.15 M NaCl) with 0.3% Tween 20 (Fisher Scientific, Cat# BP337-100)(TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... Each cell line was tested in 96 well plates (Thermo Scientific™, Cat# 167425) at 5 K cells/well in the culture media ...
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bioRxiv - Biophysics 2024Quote: ... samples were analyzed using an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific). The MS/MS spectra obtained were searched against the UniProt (Xenopodinae ...
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bioRxiv - Biophysics 2024Quote: ... Quantitative real-time PCR (qPCR) was performed in triplicates in a QuantStudio 6flex (Thermo Fisher) using PowerUp SYBR green master mix (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... or streptavidin (1:5000, Thermofisher, S911). Membranes were then incubated for 3 min with Amersham ECL TM Western Blotting detection reagents (Sigma ...
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bioRxiv - Biophysics 2024Quote: Quantitative determination of intracellular pH (pHi) was performed using the cell-permeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid AM (Thermo Fisher) in live imaged N2a cells at 48 hours of differentiation ...
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bioRxiv - Biophysics 2024Quote: ... GFP (1:2000, Invitrogen, rabbit polyclonal, A6455), myc (1:1000 ...
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bioRxiv - Biophysics 2024Quote: ... in vivo pHi calibration was performed by fixing the pHi between 5.5 and 7.5 with the commercially available Intracellular pH Calibration Buffer Kit (Thermo Fisher). Valinomycin and nigericin were used to equilibrate the intracellular pH ...
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bioRxiv - Biophysics 2024Quote: ... and extracts were incubated with MyOne Dynabeads Streptavidin C1 (Invitrogen). The beads were then washed with a subsequent sequence of wash buffers ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were normalized using the Pierce 660 nm protein assay (Thermo Scientific; #22662) with the ionic detergent compatibility kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... as well as 5 μl DNase I (Thermo Fisher #90083). 15 ml RPMI with 10% FBS was then added to stop the dissociation process ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... Anti-Mouse AlexaFluor-647 (Thermo Fisher Scientific ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were incubated for two hours at room temperature at 1:500 dilution [Anti-Rabbit AlexaFluor-488 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were resolved by SDS-PAGE and proteins were transferred onto nitrocellulose or PVDF membranes using the iBlot2 Dry Blotting System (Thermo Fisher, IB21001, IB23001).
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bioRxiv - Neuroscience 2024Quote: ... 5 μL 10× DNAse I Incubation Buffer (Life Technologies, Carlsbad, CA, United States), 5 μL DNase I (2 U/μL) ...
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bioRxiv - Cancer Biology 2024Quote: ... all at 1:1000 in PBS with 0.1% Tween and 1 ng/ml DAPI: goat anti-rabbit AF647 (Invitrogen; A-21245) and goat anti-rat AF647 (Invitrogen ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... and separated on a BOLTTM 4-12% Bis-Tris 1mm gel (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... lysed using LDS sample buffer (Invitrogen; NP0007) with 2% beta-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:50 of goat anti-mouse AF546 (Invitrogen A11030) in PBST ...
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bioRxiv - Neuroscience 2024Quote: ... the concentration and purity of nucleic acids were assessed using a NanoDrop One spectrophotometer (Thermo Fisher Scientific). RNA integrity was evaluated using an Agilent 6000 NanoKit on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... after which secondary antibody detection was performed with 1:50 of goat anti-rat AF488 (Invitrogen A11006) and 1:50 of goat anti-mouse AF546 (Invitrogen A11030 ...
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bioRxiv - Cancer Biology 2024Quote: ... all at 1:1000 in PBS with 0.1% Tween and 1 ng/ml DAPI: goat anti-rabbit AF647 (Invitrogen; A-21245), then washed 3 times with PBS and mounted using ProLong Gold (Invitrogen) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: CytoView 48-well MEA plates (Axion Biosystems, M768-tMEA-48B) were coated with 80ug/mL Matrigel (Thermo Fisher Scientific, Cat#: 08-774-552) and seeded with human fetal astrocytes (ScienCell ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were dissociated by shaking for 30 minutes in a 37°C water bath in 5 ml of M199 media (Thermo Fisher 11150067) containing 1 mg/ml each of Collagenase Type 1 (Worthington Biochemical LS004216) ...
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bioRxiv - Developmental Biology 2024Quote: ... were employed in the evaluation of AMATERAS-2w (Figs. 1C-D) and those with a 0.5 µm diameter (FluoSphere F8813, Invitrogen, ThermoFisher Scientific, Waltham, MA) were employed in the evaluation of AMATERAS-2c (Figs ...
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bioRxiv - Biochemistry 2024Quote: ... or with the Orbitrap Exploris 480 mass-spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a FAIMS (high Field Asymmetric Ion Mobility Spectrometry ...
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bioRxiv - Biochemistry 2024Quote: ... 5 ng/ml epidermal growth factor (Gibco BRL), 0.2 μMg/ml dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... and 40 μMg/ml gentamicin (Gibco/Life Technology). The C25 myoblast cell line obtained from Institut de Myologie (Paris ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1% sodium deoxycholate] with a protease inhibitor cocktail (78444, Thermo Fisher Scientific), and protein amounts were quantified using a BCA protein assay kit (23227 ...
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bioRxiv - Biochemistry 2024Quote: ... with Proteome Discoverer software (Thermo Fisher Scientific). Peptides and proteins were filtered with a false discovery rate (FDR ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were identified by database searching using SequestHT (Thermo Fisher Scientific) with Proteome Discoverer software (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: Jurkat E6.1 cells (ECACC #88042803, RRID: CVCL_0367) were grown in RPMI (Gibco) medium completed with 10% FBS (Gibco BRL ...
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bioRxiv - Cancer Biology 2024Quote: ... with the ionic detergent compatibility kit (Thermo Scientific; #22663) using BSA as a standard ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed 3 times with PBS and mounted using ProLong Gold (Invitrogen). For quantitative image-based cytometry ...
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bioRxiv - Cancer Biology 2024Quote: ... via cytocentrifugation on a Shandon Cytospin 3 Cytocentrifuge (Thermo Fisher Scientific) at 500g for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 µl of saturated cell culture (2 million cells/ml) was mounted on a frosted microscope slide (Fisher Scientific #1255015) via cytocentrifugation on a Shandon Cytospin 3 Cytocentrifuge (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then counted using Countess 3 Cell Counters (ThermoFisher). CellTiter-Glo (#G9242 ...
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bioRxiv - Cancer Biology 2024Quote: ... the purified PCR product was cloned into the pCR2.1-TOPO TA vector (TOPO TA cloning kit; Life Technologies) and sequenced by Sanger sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA ligase 4 (ThermoFisher Scientific #PA5-40826), PARP1 (Santa Cruz Biotechnology #sc-74470) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 1 hr incubation with secondary antibodies anti-mouse Alexa Fluor 594 (1:500, #A11062, Life Technologies) and anti-rat Alexa Fluor 488 (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 U/mL penicillin-100 µg/mL streptomycin (Gibco, 15140122), 10 mM HEPES (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Between 10 to 30 µg of protein were prepared in Laemmli buffer boiled for 5 min and proteins size separated by SDS-PAGE using NuPAGE 10% Bis-Tris 1.5 mm gels (Life Technologies). Proteins were then transferred onto nitrocellulose membranes (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and complementary DNA was synthesized using SuperScript First Strand Synthesis SuperMix (Thermo Fisher Scientific), according to the respective manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM sodium pyruvate (Gibco, 11360070), 100 U/mL penicillin-100 µg/mL streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... washed in PBS and stained with green fixable viability dye (Thermo Fisher Scientific). Cells were fixed for 1 h using the eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% FCS (Gibco), 1 mM L-glutamine (Gibco ...