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Citations for Thermo Fisher :
2701 - 2750 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Microbiology 2020Quote: ... Expression and purification of recombinant proteins were performed according to manufacturer’s instructions (Invitrogen Corporation, Catalog no. K1710-01): YPD medium supplemented with 100 μg ml−1 zeocin was used for initial growth of P ...
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bioRxiv - Neuroscience 2020Quote: To label purified recombinant tau (monomeric or aggregated tau P301S) with Dylight 488 NHS ester (Thermo Fisher Scientific) or pH-sensitive form of rhodamine (pHrodo ...
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bioRxiv - Immunology 2022Quote: ... The recombinant product was transformed into MAX Efficiency™ Stbl2™ Competent Cells (Thermo Fisher Scientific, MA, USA) to generate the pNDV-HXP-S rescue plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... HMLE.M cells were induced after prolonged treatment with 2.5 ng/ml recombinant TGFβ1 (Life Technologies, Cergy-Pontoise, France). Cell proliferation and survival analyses were performed in 3 or more independent experiments ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Synthetic Biology 2019Quote: ... The soluble recombinant MDH mutants were purified by using HisPur™ Ni-NTA Spin columns (ThermoFisher Scientific, USA). The columns with loaded supernatants were washed with Wash buffer (0.1 M Potassium phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Immunology 2020Quote: ... Recombinant viruses were generated in Vero E6 cells (ATCC-CRL1586) grown in DMEM high glucose media (Gibco #11995065) supplemented with 10% Hyclone Fetal Clone II (GE #SH3006603HI) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Immunology 2022Quote: ... Recombinant baculoviruses were produced using the Bac-to-Bac baculovirus expression system according to the manufacturer’s manual (Invitrogen).The scheme for constructing the recombinant baculoviruses ...
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bioRxiv - Microbiology 2022Quote: ... Bacmids and recombinant baculoviruses were generated from the pFastBac Dual vectors using the Bac-to-Bac system (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Pathology 2023Quote: Tissue microarrays were stained using the GPER recombinant rabbit monoclonal antibody clone:20H15L21 (Thermo Fisher Scientific, catalog #703480) by DCL Pathology (Indianapolis ...
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bioRxiv - Neuroscience 2022Quote: ... Triturated tissue was then digested for 5 min using the recombinant protease TrypLE Express (ThermoFisher Scientific, cat. #12605) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB10 with DNA isolated from recombinant strains (via the Whole Blood Genomic DNA Purification Kit, Thermo Scientific) followed by selection on LB agar with the appropriate drug ...
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bioRxiv - Immunology 2023Quote: ... was made by mixing 2.5ug of IL-2 monoclonal antibody (JES6-1A12, 2B Scientific) with 7.5ug recombinant IL-2 (ThermoFisher) in 200ul of PBS for 20 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and the concentration of the recombinant plasmids was determined using the Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851). 293FT cells were transfected with 1 µg of recombinant plasmids or blank vectors with 40 ng of pRL-TK Renilla luciferase control vector using Lipofiter ...
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bioRxiv - Genomics 2023Quote: ... residual RNA contamination was removed by incubating the samples in 10µL of 1mg/mL recombinant RNase (Ambion, #AM2269). Sample aliquots of 100µL were kept at -80 °C.
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 0.134% Triton X-100 [Sigma], 0.5 U/µL recombinant RNase inhibitor [Takara, 2313B], 3.33 mM each dNTP [ThermoFisher, R1121] ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant antibodies were transiently expressed in both Expi CHO and Expi293 cells according to manufacturer’s protocol (Invitrogen). The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Evolutionary Biology 2023Quote: ... secondary antibody (goat anti-mouse IgG H + L superclonal recombinant secondary antibody with Alexa Fluor 488; Invitrogen #A28175) 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with a mixture of ketamine/xylazine and then infected via retroorbital injection of 100µl containing 1×105 genome equivalents (G.E.) of recombinant NrHV diluted in DPBS (Gibco) (N = 5/strain) ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The recombinant plasmids were transfected into 293T cells using LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA). For RT-qPCR ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies and recombinant RBD-hFc proteins were purified using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424). In general ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant baculoviruses were prepared in the Spodoptera frugiperda (sf9) cells using the Bac-to-Bac system (Life Technologies). HIF2α and BMAL1 were co-expressed in Trichoplusia ni Hive Five cells by infection of 25 ml each of baculoviruses per 1 L of High Five culture ...
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bioRxiv - Immunology 2024Quote: Recombinant rabbit mAbs and Fabs were expressed in HEK293F cells using ExpiFectamine™ 293 Transfection reagent (Gibco, A14525). Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was in vitro transcribed from the linearized recombinant infectious clone using the mMESSAGE mMACHINE Kit (Ambion) and electroporated into Vero E6 cells ...
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bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to a Q Exactive hybrid mass spectrometer (Thermo) (N-terminal FLAG-tagged datasets) or an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer (C-terminal FLAG-tagged datasets) ...
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bioRxiv - Developmental Biology 2019Quote: ... C- and N-terminal GFP-tagged Nubp1 and Nubp2 expression plasmids [32] were transfected using Lipofectamine® 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Nikon C2 Confocal microscope.
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bioRxiv - Biochemistry 2019Quote: ... Site-directed mutagenesis of plasmids to generate the C-terminal Myc tagged ECH1 catalytic mutants and the C-terminal V5 tagged UCP3 truncation mutants was carried out with Platinum pFX DNA polymerase from (Life Technologies, Grand Island, NY), following the manufacturer’s protocols for site-directed mutagenesis ...
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bioRxiv - Genomics 2022Quote: ... we performed end repair and then selectively purified biotin-tagged DNA using streptavidin coated beads (DYNAL™ MyOne™ Dynabeads™ Streptavidin C1, Invitrogen 65001). A-tailing and Illumina TruSeq adapter ligation (Illumina ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).