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Citations for Thermo Fisher :
2601 - 2650 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Molecular Biology 2020Quote: ... All of the UMI-tagged template molecules were added to the reaction containing 2 U Platinum SuperFi DNA Polymerase High Fidelity (Thermo Fisher Scientific, USA), and a final concentration of 1X SuperFi buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488- or Alexa-Fluor-568-tagged donkey anti-mouse- or anti-rabbit-immunoglobulin antibodies (A32766, A32790, A10042, A10037, Molecular Probes, 1:200) and Alexa-Fluor-633 goat anti mouse (A-21050 ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Physiology 2022Quote: ... HPAECs were transfected with either control plasmids (empty vector) or 1μg of HA-ubiquitin plasmids (HA-tagged Ubi) using Lipofectamine 3000 (Thermo fisher Scientific, PA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed three times with 1X PBS and incubated with suitable fluorophore tagged secondary antibodies (1: 2000, Invitrogen, San Diego, CA, USA). Cells were washed with 1X PBS before mounting ...
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bioRxiv - Microbiology 2024Quote: ... Detection of the murine-human chimera 72A1 detection was performed using goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor™ 488 tagged (Invitrogen #A-11001).
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bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
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bioRxiv - Molecular Biology 2024Quote: T7 double stranded DNAs (T7-dsDNAs) were amplified by T7-tagged primer pairs (Table 2) by Phire Hot Start II PCR Master Mix kit (Thermo Fisher, Cat # F125L) using proper plasmids as templates ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice with phosphate-buffered saline for 5 minutes and incubated with Alexa Fluor 555-tagged donkey-anti-goat (Molecular Probes, 1:1000) and Alexa Fluor 647-tagged donkey-anti-rabbit (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... 1 x 107 pfu of mRFP-VP26 tagged virions or 1 x 108 of Yellow-Green fluorescent tagged nanoparticles (160 nm, FluoSpheres™ Carboxylate-Modified Microspheres Catalog #F8811, Thermo Fisher Scientific) were added to the dish ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed with 0.1% PBS-TX three times and treated with Alexa Fluor 488-tagged goat-anti-rabbit IgG antibodies (Thermo Fisher, Cat # A-11008), diluted 1:800 in 0.1% PBS-TX for 6–12 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: HEK293T or HeLa Kyoto cells expressing the indicated tagged proteins were washed twice with ice-cold PBS and lysed in M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) containing protease and phosphatase inhibitors ...
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bioRxiv - Microbiology 2020Quote: 3 ⨯106 cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Immunology 2019Quote: ... and human bronchial carcinoma Calu3 epithelial cells (ATCC_HTB-55) were grown in 10% FCS in alpha MEM media (Gibco). Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185 ...
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bioRxiv - Immunology 2019Quote: ... Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185) were grown in 10% FCS with 1% L-glutamine in Hams/F-12 media (Gibco).
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bioRxiv - Biochemistry 2020Quote: ... The absorbance of each well at 450 nm was measured using a Multiskan FC microplate photometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... The culture medium was removed and replaced with RPMI 1640 medium with 10% CS-FCS (Gibco® Life Technologies) and cells were incubated with or without 50 nM T3 for another 3 days ...
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bioRxiv - Cancer Biology 2019Quote: ... The culture medium was removed and replaced with RPMI 1640 medium with 10% CS-FCS (Gibco® Life Technologies) and cells were incubated with or without 50 nM T3 for another 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... The amount of ingested blue dye in the supernatant was measured spectrophotometrically at 620 nm (Fisher Scientific Multiskan FC). Sample readings were normalized to the number of flies.
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bioRxiv - Microbiology 2020Quote: rS1-Fc was expressed in Chinese hamster ovary cells (Free Style CHO-S Cells by Thermo Fisher Scientific, # R80007) in CHO-S-SFM media with hypoxanthine and thymidine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... EVs were resuspended in D-MEM (0 % FCS) + fluorescent Bodipy Phosphocholine analogue at 2 μg/mL (D3793, Thermofisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: Cryosections were permeabilized and blocked for 1 h at RT in 0.1% PBTx and 10% fetal calf serum (FCS; Invitrogen), and incubated O/N at 4 °C with the primary antibodies in 0.1% PBTx plus 1% FCS ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (P/S, 10.000 U/ml, Gibco). Jurkat ...
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bioRxiv - Microbiology 2021Quote: ... or 20% (THP-1) (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (10.000 U/ml, Gibco). THP-1 monocytes were treated with 100 nM 12-O-tetradecanoylphorbol-13-acetate (TPA ...
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bioRxiv - Cell Biology 2021Quote: Dulbecco’s modified Eagle’s medium (DMEM) and DMEM-F12 containing 10% heat-inactivated fetal calf serum (FCS) (Thermo Fisher Scientific) were used to culture human HEK293T ...
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bioRxiv - Immunology 2022Quote: ... Extracellular antibodies were diluted in FACS buffer (2% FBS and 5mM EDTA in PBS) with Fc Block (Life Technologies) and incubated for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... cell pellet was re-suspended in ice-cold proliferation growth medium (PGM) containing DMEM supplemented with 10% FCS (Invitrogen), 10% horse serum (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... and grown for 4 days on untreated tissue culture plates in a humidified incubator with 5% CO2 at 37°C with culture medium (RPMI, 10% heat-inactivated FCS, 50 units/mL penicillin; 50μg/ml streptomycin -GIBCO) supplemented with 50 ng/ml rhM-CSF (Sino Biologicals) ...
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bioRxiv - Microbiology 2022Quote: ... with 7⍰.5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000μg.mL−1 respectively; Life Technologies) and supplemented with 1⍰% non-essential amino acids (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (Dulbecco’s phosphate-buffered saline [Gibco] supplemented with 2% fetal calf serum (FCS) ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse embryonic fibroblasts (MEF) were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS, GIBCO), 10 U/ml penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... Germany) were cultivated in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FCS, Biochrom or GIBCO), 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech) ...
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bioRxiv - Microbiology 2021Quote: ... J/4320/15) and the absorbance at 450 nm was read on a Multiskan FC Microplate Photometer (Thermo Scientific). Titres were determined by curve fitting data to a 4PL curve in GraphPad Prism 9 and accepting only data which produced an R2 value of >0.95 ...
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bioRxiv - Microbiology 2021Quote: ... 30-2003, ATCC) supplemented with 10% heat-inactivated fetal calf serum (FCS, Hyclone, USA) and 1% pen/strep (Invitrogen) at 37°C in a humidified 5% CO2 incubator (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Developmental Biology 2022Quote: ... These cells were washed with PBS and then resuspended in PBS supplemented with 2% FCS or FBS and 5µM CM-H2DCFDA probe (Invitrogen) and incubated in the dark for 20min at 37°C under 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... with 7 .5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... They were cultured in RPMI 1640 medium supplemented with l-glutamine and 10% FCS (v/v) (Gibco, Massachusetts, USA). Vδ2+ γδ T cell expansion was stimulated using 5 μM zoledronic acid (Actavis ...
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bioRxiv - Cell Biology 2022Quote: ... The absorbance of each well was measured with a microplate reader (Multiskan FC, Thermo Fisher Scientific, Waltham, MA, USA) set to 450 nm ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed 6x 300μl PBST and developed with an anti-human Fc specific secondary antibody (Invitrogen, Carlsbad, CA) and TMB as described above ...
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bioRxiv - Immunology 2019Quote: ... The plate was incubated with detection antibody (mouse anti-human IgG1 Fc conjugated to horseradish peroxidase clone HP6069; ThermoFisher) for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... pH 7.2) resuspended and incubated for 30 min in FACS buffer supplemented with Fc gamma receptor CD16/CD32 antibodies (ThermoFisher). Cells were then incubated with Alexa fluor 488-conjugated antibodies against F4/80 or rat IgG2a kappa isotype control ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were immersed in 2ml dissociation solution (2% FCS-PBS solution with approximate 145U/ml type 4 Gibco collagenase) and incubated at 37°C for 30 minutes ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... filtered through a 70 µm cell strainer, washed with FACS buffer (PBS supplemented with 2% FCS, 2 mM EDTA and 0.01% Sodium Azide (all Gibco)) and maintained at 4°C ...
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bioRxiv - Immunology 2021Quote: ... in R10 media (RPMI 1640 with 10% FCS, 1% penicillin/ streptomycin, and 2 mM L-glutamine, Thermo Fisher Scientific) at 37°C in 5% CO2 to differentiate quiescent MBCs into mASCs ...