Labshake search
Citations for Thermo Fisher :
2651 - 2700 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... FACS-sorted cells were suspended at 0.5×106 cells/mL in RPMI 1640 medium supplemented with 10% HI-FCS and 1% PenStrep (10378016; Gibco). CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well ...
-
bioRxiv - Immunology 2021Quote: ... The hACE-2-Fc content in the supernatant was quantified by ELISA using 96-Well MaxiSorp Immuno plates (ThermoFisher) coated overnight with 200 ng/well of RBD219-WT protein ...
-
bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
-
bioRxiv - Immunology 2022Quote: ... as per the manufacturer’s instructions.(Heideveld, Hampton-O’Neil et al. 2018) CD14+ cells were stored frozen in 50% foetal calf serum (FCS, Gibco), 40% PBS with 10% DMSO (Sigma ...
-
bioRxiv - Developmental Biology 2022Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
-
bioRxiv - Cell Biology 2022Quote: ... for 10 min at 37°C followed by trituration in DMEM supplemented with 10% FCS and penicillin/streptomycin (Gibco). Dissociated cells from one embryonic heart were plated on one glass coverslips (diameter 12 mm ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Idiotype antibodies and Fc-proteins were fluorophore-conjugated using DyLight 650 or DyLight 488 Microscale Antibody Labeling Kits (Invitrogen). ROR1 scFvs also contained a VSV-g tag that were detected by anti-VSV-g polyclonal antibody (FITC ...
-
bioRxiv - Cancer Biology 2024Quote: ... BE(2)-C cells and SK-N-AS cells were grown in 75 cm2 cell culture flasks in Dulbecco’s Modified Eagle’s Medium (DMEM) + 10% fetal calf serum (FCS) (Life Technologies), and stable ATP13A3 KD and overexpression SH-SY5Y cells were cultured in DMEM with high glucose supplemented with sodium bicarbonate ...
-
bioRxiv - Physiology 2024Quote: For MPS 4.0×104 NIH-3T3 fibroblasts were seeded onto 24-well plates and cultured in 10 % FCS/ DMEM (Gibco). Cells were treated for 60 min (at 37 °C and 50 rpm ...
-
bioRxiv - Cell Biology 2024Quote: ... The absorbance of wells was subsequently measured at 450 nm using a Multiskan FC microplate reader (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2024Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
-
bioRxiv - Microbiology 2024Quote: Growth was assessed by measuring absorbance at 595 nm over time in a Multiskan FC microplate photometer (Thermo Scientific) using flat-bottom 96-well plates (Bioké ...
-
bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits, mouse IgG2a for anti-EGFR 199.12 quantitation [ThermoFisher Z25108] ...
-
bioRxiv - Cell Biology 2023Quote: Kidneys were harvested and transferred to MEM with Earle’s Salts (with 2.2 g/l NaHCO3, without L-Glutamine) medium (Biochrom) supplemented with 10% FCS (Life Technologies), 1x Non-Essential Amino Acids (NEAA ...
-
bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
-
bioRxiv - Microbiology 2023Quote: HeLa and HEK293T cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) with GlutaMAX and 10% decomplemented-FCS (fetal calf serum) (Gibco, Life Technologies). HeLa WT and HeLa BST2 KO cells were a kind gift of Stéphane Fremont (Institut Pasteur ...
-
bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
-
bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody used was R-Phycoerythrin labelled Goat anti-Human IgG Fc (eBioscienceTM; 12-4998-82, Invitrogen).
-
bioRxiv - Biochemistry 2023Quote: ... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... autoclaved and then supplemented with 0.2 to 1 mL (10% final) of fetal calf serum (FCS, Gibco Thermo Fisher); and 0.2-1 mL (10% final ...
-
bioRxiv - Microbiology 2023Quote: ... autoclaved and then supplemented with 0.2 to 1 mL (10% final) of fetal calf serum (FCS, Gibco Thermo Fisher); and 0.2-1 mL (10% final ...
-
bioRxiv - Microbiology 2023Quote: Human pharyngeal carcinoma Detroit 562 epithelial cells (ATCC_CCL-138) were grown in 10% FCS in alpha MEM media (Gibco). For each set of experiments ...
-
bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... the remaining cells were stained for sorting in PBS containing 0.5% FCS at 4L°C with the following antibodies: Streptavidin-AF488 (1:100, Invitrogen), CD45-BV510(1:50 ...
-
bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... the corresponding cells were grown to near confluency (cultured in DMEM with 10% FCS (Benchmark) and 1% PSG (Gibco)) and harvested using 0.05% Trypsin-EDTA (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... A459 cells were grown in RPMI 1640 with L-glutamine and 10% heat-inactivated fetal calf serum (FCS, Gibco). Penicillin and streptomycin were added for routine culture but were removed the day before and during experiments.
-
bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
-
bioRxiv - Immunology 2024Quote: ... final volume of 200 µl RPMI 1640 containing 10% FCS and penicillin/streptomycin and 55 µM β-mercaptoethanol (Gibco) for the stated time periods in the presence of a range of [4Y]-MBP peptide concentrations ...
-
bioRxiv - Immunology 2024Quote: ... lymph node suspensions at 107 cells per mL in PBS 0.5%FCS were labelled with 5 µM CTV (Invitrogen) for 20 min at 37°C before reaction was quenched and cells washed with cold medium ...
-
bioRxiv - Bioengineering 2024Quote: ... HUVEC samples were treated with an Fc receptor blocking reagent (1 test) (Thermo Fisher Scientific, Cat. # 14-9161-73) and incubated at 4°C for 15 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... with 7.5% heat-inactivated Fetal Calf Serum (FCS; Life Technologies with 1% penicillin/streptomycin PS, 5000U.mL−1 and 5000µg.mL−1 respectively (Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies) ...
-
bioRxiv - Molecular Biology 2024Quote: The media used during the live imaging is the Gibco™ FluoroBrite™ DMEM complemented with 10% FCS (GIBCO) and 1% penicillin-streptomycin (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
-
bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
-
bioRxiv - Developmental Biology 2021Quote: ... RT-PCR was performed to obtain cDNA using Superscript III reverse transcriptase and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). QPCR was further performed using Bio-Rad SsoAdvanced universal SYBR green supermix on the Biorad CFX Connect real-time PCR system ...
-
bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
-
bioRxiv - Cancer Biology 2021Quote: Recombinant STUB1 protein (aa25-aa153) was dialyzed overnight with Slide-A-Lyzer cassette (7K MWCO, Thermo Scientific, #66373) in 1 liter of dialysis buffer (PBS ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
-
bioRxiv - Microbiology 2022Quote: ... The recombinant constructs were transfected into HEK293 cells either individually or together using Lipofectamine® 3000 reagent (Invitrogen) following the instructions provided ...
-
bioRxiv - Immunology 2022Quote: ... Recombinant antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described (Lorin and Mouquet ...
-
bioRxiv - Microbiology 2022Quote: ... supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) plus 2% penicillin-streptomycin (Gibco) at 37°C in 5% CO2 ...
-
bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
-
bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
-
bioRxiv - Biophysics 2022Quote: ... The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al., 2017) (Thermo Fisher Scientific). The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...