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Citations for Thermo Fisher :
2851 - 2900 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood as described for the PBMCs from the patients and cryopreserved at -150°C in fetal calf serum (FCS, Gibco) with 10% dimethyl sulfoxide (DMSO ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... were cultured for 24h in 100 μL of RPMI medium containing 10% FCS in the presence of 5 μL of anti-CD3/CD28 beads (ThermoFisher). After centrifugation (450g ...
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bioRxiv - Microbiology 2023Quote: HeLa and HEK293T cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) with GlutaMAX and 10% decomplemented-FCS (fetal calf serum) (Gibco, Life Technologies). HeLa WT and HeLa BST2 KO cells were a kind gift of Stéphane Fremont (Institut Pasteur ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then washed and incubated with 1 μg/ml DAPI/20% FCS/0.3% TritonX-100/PBS and either anti-rabbit Alexa Fluor 488 (Invitrogen, A11070) and anti-mouse Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Freshly FACS-sorted cells were plated into 96 wells at 400,000 cells per well for each cell type and cultured for 24-48 hours in 200 ul of RPMI medium with 10% FCS (Gibco), 2 mM glutamine (ThermoFisher) ...
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bioRxiv - Physiology 2023Quote: ... and grown in DMEM/F12 with 10% FCS and penicillin–streptomycin (Life Technologies, final conc., 50 U/mL of penicillin). Differentiation was achieved by culturing the cells in differentiation media (DMEM/F12 with 2% horse serum and penicillin–streptomycin) ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL 1:5000 Goat anti-Human IgG Fc Secondary Antibody with HRP (Invitrogen) for one hour at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed 5x and incubated with 100 µl/well with an HRP-conjugated anti-human IgG Fc antibody (1:10000 dilution, Invitrogen) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 1:7500 goat anti-rabbit IgG A680nm and 1:7500 goat anti-mouse IgG A790nm for 1 hour at room temperature before being visualised on LiCor Odyssey Fc (Invitrogen).
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bioRxiv - Immunology 2023Quote: Surface antibody staining was conducted in PBS containing 0.1% FCS and 2mM EDTA for 30 minutes at 4°C to cells that had been pre-incubated with Fc block and LIVE/DEAD Blue (InVitrogen) staining ...
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bioRxiv - Immunology 2023Quote: ... and B16 were cultured inside tissue culture treated flasks with DMEM with 10% FCS (Hyclone) and 1X penicillin-streptomycin (100 units/ml, Gibco) at 37C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... All FCS measurements were carried out at ∼20 nM fluorescently labeled protein at 20°C in Nunc chambered coverslips (ThermoFisher). The chambers are pre-incubated with poly-lysine conjugated polyethylene glycol (PLL-PEG ...
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bioRxiv - Genetics 2023Quote: ... Cells were grown in DMEM with 10% FCS and penicillin–streptomycin (Life Technologies, final conc., 50 U/mL of penicillin). Differentiation into mature brown fat adipocytes was achieved by adding an adipogenic cocktail (0.5 μg/mL insulin ...
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bioRxiv - Developmental Biology 2023Quote: siRNA knockdown was performed as previously reported.20 9-12 week placental explants were placed into a 48-well plate with IMDM supplemented with 10% (v/v) FCS and gentamicin (50 μg/mL; Gibco). siRNA sequences targeting PRKCI (final concentration 0.2 μM ...
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bioRxiv - Immunology 2023Quote: Non-specific Fc binding was blocked for 10 minutes on ice with unconjugated anti-CD16/CD32 (93; Thermo-Fisher Scientific). Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were washed with PBS and resuspended in 200 µl buffer (PBS supplemented with 2% FCS) and analyzed via Attune NxT Flow Cytometer (ThermoFisher). For LIVE/DEAD analysis all cells were gated and viable cells were quantified as indicated in Figure 4 via FlowJo (Version 10.9.0 ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were then incubated with a 1:10,000 dilution of goat anti-human Fc HRP secondary antibody (Invitrogen, A18817) in PBST for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... cells in each well were firstly washed with flow cytometry staining buffer: 3% FCS with 2 mM EDTA (15575020; Invitrogen) in PBS− ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with anti-CD16/32 (FC receptor block) at 1:100 and LIVE/DEAD Fixable (1:400-1:800; cat no., 15519340; Invitrogen) in 20 μl PBS for 10 min ...
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bioRxiv - Immunology 2023Quote: ... to block non-specific binding of fluorochrome-conjugated anti-human monoclonal antibodies to Fc receptors before being labeled with a 27-color panel of markers including the viability dye Live/Dead Blue (Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 4mM EDTA and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), CD4-AF488 (clone GK1.5 ...
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bioRxiv - Genetics 2023Quote: ... resuspended in 800 μL of PBS supplemented with 2 % FCS and analyzed on Attune NxT Flow Cytometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were targeted directly using an anti-ALFA nanobody carrying a 5xR1 docking strand (5-TCCTCCTCCTCCTCCTCCT-3’) (Massive-TAG-Q Anti-ALFA Custom, Massive Photonics, GmbH) which was diluted in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies rabbit anti-HA (1:500) was subsequently added in permeabilization/blocking buffer (10% fetal calf serum (FCS) (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... E14 mouse embryonic stem cells were cultured in plates coated with 1% gelatine in GMEM supplemented with 10% foetal calf serum (FCS) batch-tested for ES cell culture (Gibco), 0.1 mM non-essential amino acids ...
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bioRxiv - Physiology 2023Quote: ... nearly 106 cells were first incubated on ice for 30 minutes in a solution consisting of a 1:10 Fc Receptor Binding Inhibitor (eBiosciences) and 1:500 Live/Dead Aqua stain (Invitrogen) diluted in PBS ...
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bioRxiv - Immunology 2024Quote: ... and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS) and 100 U/ml of rhIL-2 (Gibco). 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS; Capricorn Scientific, Ebsdorfergrund, Germany) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Dreireich, Germany) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagen plugs were set at 37°C/5%CO2 for 10 minutes, and then fibroblast growth medium was added (DMEM medium supplemented with 10% FCS, 2 mM L-glutamine (ThermoFisher) and 100U/mL penicillin/streptomycin (ThermoFisher)) ...
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bioRxiv - Microbiology 2024Quote: ... and HKU1 NTD Fc + pA-LS at 50 μg/mL were incubated with StrepMAB-Classic (IBA) and goat αnti-mouse IgG HRP (Invitrogen) at a 4:2:1 molar ratio for 30 min on ice ...
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bioRxiv - Microbiology 2024Quote: The binding of KIRs-Fc fusion proteins to iRBCs was assessed by flow cytometric analysis with Attune NxT (ThermoFisher Scientific). Prior to the assay ...
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bioRxiv - Immunology 2024Quote: ... BHK21 cells were purchased from ECACC (85011433) and cultured in DMEM (supplemented with 10 % FCS, 10 mM HEPES (Gibco, 15630056), 1 mM Na-Pyruvate (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... washed once with PBS and mixed with a total of 10 μg recombinant DNA in electroporation buffer R (Invitrogen). Cells were singularly electroshocked at 1600mV for 20 msec by placing the electroporation tip into the column filled by E2 buffer (following manufacturer specifications) ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2019Quote: ... PBMCs were cultured for 9 to 12 days in an erythroblast enrichment medium [38] to expand the erythroblast population and 2.5×105 cells were transduced using recombinant Sendai viral vectors (Cytotune-iPSC 2.0TM, Thermofisher scientific), expressing the four reprogramming factors Oct4 ...
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bioRxiv - Microbiology 2019Quote: The PfCHC-2xFKBP-GFP soluble extract in buffer B was incubated with 4μl recombinant anti-GFP nanobodies covalently coupled to surface-activated Epoxy magnetic beads (Dynabeads M270 Epoxy, ThermoFisher) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... in OT-1 culture medium supplemented with 100 U/ml mouse recombinant IL-2 (Thermo Fisher Scientific, catalog 14802164) in a 37 °C/5% CO2 incubator ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in B8 media with varying concentrations if iMatrix laminin and/or truncated recombinant human vitronectin (ThermoFisher #A14700). Cells were seeded at 5,000 cells/cm2 ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Immunology 2020Quote: ... The vector was linearized with BglII and transformation of yeast (Pichia pastoris) and induction of recombinant protein expression was carried out according to the manufacturer’s instructions (Invitrogen). Recombinant HELMCC protein was purified from yeast culture supernatants by ionexchange chromatography using Ni2+ affinity chromatography (Amersham Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were purchased from Kerafast and grown in keratinocyte serum-free media supplemented with bovine pituitary extract and recombinant epidermal growth factor (Gibco, #37010022) with 1% Penicillin-Streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant lentiviral and retroviral vectors produced from previous steps were amplified using the GeneJET Plasmid Maxiprep Kit (Thermo Scientific). The 3rd generation lentiviral (pRSV.REV ...
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bioRxiv - Neuroscience 2020Quote: ... Some mice received a mixture of muscimol and Cholera Toxin Subunit B (Recombinant) Alexa Fluor (647 conjugate, Thermo Scientific) to localize site of infusion ...
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bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
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bioRxiv - Cell Biology 2020Quote: ... containing the two recombinant Gateway sites attB1.1 and attbB.1 and subcloned into a pDONR/Zeo Gateway Donor vector (Invitrogen) as the entry clones ...