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Citations for Thermo Fisher :
2501 - 2550 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... was used to measure the m6A level in total RNA by MultiSkan FC microplate reader (Thermo Fisher, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies ...
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bioRxiv - Physiology 2024Quote: ... The isolated cells were plated (0.1 % gelatine-coated T75 flasks, approx. 1.0×107 cells) and cultured in 10 % FCS-containing DMEM (Gibco). Medium was changed every 3 days ...
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bioRxiv - Biophysics 2023Quote: ... Both iPSCs classes were cultured on 0.1% gelatin-coated dishes in FCS/LIF medium (DMEM+Glutamax (61965059, Gibco), 15% fetal calf serum (Pansera ES ...
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bioRxiv - Microbiology 2023Quote: ... Polyclonal goat anti-pig immunoglobulin G (IgG) Fc Secondary Antibody HRP (Invitrogen by Thermo Fisher Scientific, Waltham, USA) was diluted 1:15.000 in blocking buffer and strips were incubated for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... supplemented in media (DMEM with 10% FCS and penicillin, Life Technologies, final conc. 50 U/mL of penicillin) for 2 hours at 37° C ...
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bioRxiv - Developmental Biology 2023Quote: ... Bones were flushed using a 26-gauge needle of a 1-ml syringe with HBSS+2% FCS (Gibco). BM clumps were re-suspended by gentling aspirating with a 19-gauge needle ...
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bioRxiv - Cell Biology 2023Quote: CEM were counted and resuspended at a concentration of 2-3 x 106 cells/mL in ice cold FACS buffer (PBS, 10% FCS, 2mM EDTA) containing 1.25 µL anti-human CD71 (0.625 µg/mL, OKT9, Invitrogen) for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck) ...
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bioRxiv - Bioengineering 2023Quote: ... Both cell lines were maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies) and 2mM GlutaMAX ...
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bioRxiv - Bioengineering 2023Quote: ... Both cell lines were maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies) and 2mM GlutaMAX ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with FC block for 30 minutes and stained with anti-MECA79 (53-6036-82, Invitrogen), anti-GP38 (127423 ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were incubated at 30 °C and OD600 was measured using a Multiskan FC Microplate Photometer (Thermo Fisher). For the evaluation of chemical compounds ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β3 (armenian hamster monoclonal, 2C9.G3, Thermo Fisher Scientific, 13-0611-81; FC 1:200), anti-mouse integrin α5 (rat monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β1 (Armenian hamster monoclonal, Hmb1-1, Thermo Fisher Scientific, 11-0291-82; FC 1:300), anti-mouse integrin β3 (armenian hamster monoclonal ...
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bioRxiv - Immunology 2023Quote: ... The idiotype antibodies and Fc- fusion protein were conjugated in house with Dylight650 antibody labelling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated with both WA2.22 Fc fusion (100 nM) and AT8 (1:200 dilution, Invitrogen) in M ...
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bioRxiv - Bioengineering 2023Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:10,000 ...
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bioRxiv - Immunology 2022Quote: ... S.Tm were washed with PBS and reconstituted in DMEM/F-12 (STEMCELL) supplemented with 3% FCS (Thermo Fisher). For 2D enteroid-derived monolayer infections ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Biowest Riverside) and 1% penicillin/streptomycin (Thermo Fisher Scientific), after incubation with Fc receptor blocking reagent (Miltenyi Biotech) ...
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bioRxiv - Microbiology 2023Quote: Cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... followed by three washes with 1% FCS/PBS and secondary staining using Alexa-546-anti-rabbit (Invitrogen, A11035), Alexa-488-anti-mouse (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), Epcam-BV421 (clone G8.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... were grown in DMEM (Dulbecco’s Modified Eagle’s Medium, Hyclone) supplemented with 10% (v/v) FCS (Fetal Calf Serum, Gibco), 3.7 g/l sodium bicarbonate ...
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bioRxiv - Cancer Biology 2023Quote: Viably frozen samples obtained from patients diagnosed with AML or normal CD34+ hematopoietic progenitor cells were thawed in 37°C water bath as fast as possible and placed immediately in 20% FCS Iscove’s Modified Dulbecco’s Medium (Gibco IMDM ...
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bioRxiv - Cancer Biology 2023Quote: ... suspended in 20μl sterile complete media (MEM-Alpha (Biological Industries)) supplemented with 10% Fetal Calf Serum (FCS) (Gibco) and 1% L-Glutamine/Penicillin/Streptomycin (Biological Industries) ...
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bioRxiv - Immunology 2024Quote: ... and absorbance was measured at 450 nm and 570 nm using the Multiskan FC Microplate Photometer (Thermo Scientific). To correct for optical imperfections ...
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bioRxiv - Molecular Biology 2024Quote: In order to block non-specific Fc-mediated interaction,1 μg of normal Rabbit IgG (ThermoFisher Cat: 31235) is added to the cell sample and incubated for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... and the absorbance at 750 nm was measured using a plate reader (Multiskan FC, ThermoFisher, Waltham, MA, USA). Total polyphenols content was expressed as gallic acid equivalent per gram of watermelon flesh sample.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAX [Gibco] supplemented with 5% fetal bovine calf serum (FCS; [Gibco]) and 50 μg/mL gentamicin [Gibco] ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463 ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel droplets were overlaid with recombinant organoid medium (Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: Clones of pFastBac1 constructs were used to generate recombinant bacmids in DH10Bac following the manufacturers protocol (ThermoFisher). Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus) ...
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bioRxiv - Genomics 2020Quote: ... cells were serum-starved (with 0.3% SCS) for 72 hours prior to induction of gene expression with 0.1µg/mL recombinant EGF (Gibco) or PBS for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: Recombinant PfbA (rPfbA) or BSA was coated onto 0.5-µm-diameter fluorescent beads (FluoroSphere, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit IgG antibodies (1:500 for IF) and recombinant human BMP2 (#PHC7145) were from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with 100 U/mL mouse recombinant IFN-γ (Invitrogen, Carlsbad, CA, USA) for 24 hours and subjected to flow cytometry analysis of PD-L1 expression as described above.
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...