Labshake search
Citations for Thermo Fisher :
2951 - 3000 of 3500 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...
-
bioRxiv - Neuroscience 2023Quote: Four sections from each mouse were stained using primary antibodies calbindin D28K anti-rabbit recombinant polyclonal (1:200; ThermoFisher) and parvalbumin anti-mouse monoclonal (1:200 ...
-
bioRxiv - Biochemistry 2023Quote: ... The coding sequences of the recombinant proteins were then inserted into the BamHI and EcoRI sites of the pcDNA3.1(+) vector (Invitrogen), and all clones were verified through DNA sequencing.
-
bioRxiv - Microbiology 2024Quote: Recombinant Bd37 (rBd37) protein was expressed as thioredoxin fusion protein from the pBAD/Thio-TOPO vector (Thermo Fisher Scientific), which encodes thioredoxin and histidine-tag at N-terminus and C-terminus of multi cloning site ...
-
bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... Recombinant lentivirus used to infect SW620 cells for protein expression was generated by the ViraPower Lentiviral Expression System (Invitrogen) according to the manufacturer’s instruction.
-
bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... GPI-RBD-hGM-CSF recombinant fusion protein and GPI-hIL-12 were affinity purified from CHO-S cells (Invitrogen) stably expressing the proteins using anti-human GM-CSF (clone BVD2-21C11 ...
-
bioRxiv - Microbiology 2024Quote: ... the sequence of the resulting recombinant plasmid was ascertained by Sanger sequencing techniques (Applied Biosystems™ 3130 Genetic Analyser), and the resulting data were decoded by ApE software (v2.0.36 ...
-
bioRxiv - Biochemistry 2020Quote: ... Cytosolic extracts were generated as described above and the His6-tagged proteins purified by metal affinity chromatography and gravity flow using 0.5 ml HisPur™ cobalt superflow agarose (Thermo Fisher Scientific™, Waltham, USA) according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 50μl/well 10% HCl and the absorbance was measured at 492nm (A492) using a Multiskan FC reader (Thermo Fisher Scientific). In every CovIgG-Assay determination two in-house controls ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were washed with PBS and resuspended in 100% FCS and cytospun onto coated slides using a Shandon Cytospin 4 (Thermo Scientific) at 30g for 5 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... Daudi and HL60 cells were cultured in RPMI media supplemented with 10% fetal calf serum (FCS) and 1% pen/strep (all from Thermo Fisher). Phoenix-Ampho cells were cultured in DMEM medium (Thermo Fisher ...
-
bioRxiv - Immunology 2021Quote: ... The reaction was stopped by adding 35 μl of 1 M sulfuric acid (H2SO4) and the optical density was measured at a wavelength of 450 (OD450) (MULTISKAN FC, Thermo scientific).
-
bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein bands were visualized using a LI-COR Odyssey FC Imaging system and Super Signal West Pico Chemiluminescent Substrate (Thermo Fisher). Western blots were quantified using Image Studio Software ...
-
bioRxiv - Microbiology 2019Quote: ... Large scale transient transfections for murine CRD4-7-Fc and CR-Fc were carried out using 100 µg of total plasmid DNA and 100 ml of suspension cultured Expi293F cells (Life Technologies). Supernatants were harvested at day 6 ...
-
bioRxiv - Immunology 2019Quote: ... The remaining pieces of tissues were further digested at 37°C with shaking at 180 rpm for 45 min in 2% FCS in RPMI (Life Technologies), containing 1 mg/ml collagenase Type 1 (Life Technologies) ...
-
bioRxiv - Immunology 2019Quote: ... The idiotype antibodies and Fc-fusion proteins were conjugated in house with Dylight488 and/or 650 antibody labeling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
-
bioRxiv - Microbiology 2019Quote: ... or 0.2 (for RNA sequencing and western blots) and cultured in RPMI-HEPES supplemented with 5% v/v fetal calf serum (FCS) (Invitrogen, Australia) at pH 8.1-8.2 and allowed to differentiate at 37 °C with ambient CO2 49 ...
-
bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
-
bioRxiv - Cell Biology 2021Quote: ... and were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% FCS and 100 μg/mL of hygromycin B (Invitrogen, 10687010).
-
bioRxiv - Neuroscience 2020Quote: ... washed and non-specific antibody binding was minimised by incubation for 30 min at room temperature in PBS containing 10% FCS and 0.05% Triton X-100 (all Thermofisher Scientific, UK). Cells were then incubated with rabbit anti-mouse p67Phox monoclonal antibody (1:500 ...
-
bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown in a single well of a six-well plate in 10% FCS-MEM and transfected with 3 μg of BAC plasmid with Lipofectamine 3,000 (Thermo Fisher, USA). After transfection ...
-
bioRxiv - Immunology 2020Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
-
bioRxiv - Microbiology 2020Quote: HeLa cells (ATCC CCL-2) were incubated in RPMI (Roswell Park Memorial Institute) medium containing 5% FCS (fetal calf serum, Gibco®) in an incubator with 5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... Conditional supernatants from lentivirus-infected CHO cells sorted by FACS were used to purify the FXa-Fc fusion protein using a protein G column (89927, Thermo Fisher). For in vivo testing ...
-
bioRxiv - Microbiology 2021Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (40 ...
-
bioRxiv - Microbiology 2021Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (40 ...
-
bioRxiv - Microbiology 2021Quote: ... supernatants were loaded into a 5 mL SepFast Ø11mm (Quimigen) packed with CaptureSelect™ IgG-Fc (ms) affinity resin (Thermo Scientific) connected to an Äkta Start Chromatograph (Cytiva) ...
-
bioRxiv - Microbiology 2020Quote: ... 125 µl was transferred into a clean 96 well Costar plate to measure the absorbance at 595 nm using a Multiskan FC (Thermo Scientific).
-
bioRxiv - Molecular Biology 2022Quote: ... Sections were soaked in PBS for 10 minutes before incubation in blocking solution (20% fetal calf serum (FCS, Thermo Fisher Scientific), and 20% normal goat serum (NGS ...
-
bioRxiv - Immunology 2022Quote: ~3 mL of serum from CV-A21-immunized or naïve mice was diluted in 97 mL of PBS and ran over a column pre-packed with CaptureSelect IgG-Fc (Multispecies) Affinity Matrix (ThermoFisher Scientific). IgGs were eluted with 0.1 M glycine buffer (pH 3.0 ...
-
bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... were cultured in F-12K Nut Mix medium supplemented with 5% (v/v) artificial FCS and with 0.5% (v/v) Insulin- Transferrin-Selenium (Thermo Fisher Scientific). BEAS-2B (Cat# CRL-9609TM ...
-
bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... HCT116 colorectal adenocarcinoma cells were maintained in McCoy’s medium supplemented with 10% heat-inactivated fetal calf serum (FCS, GIBCO, Cat#16141079), 10 U/ml penicillin/streptomycin ...
-
bioRxiv - Biophysics 2020Quote: ... The amount of neutral red taken up was determined by measuring the absorbance at 540 nm using a Multiskan FC Microplate Photometer (Fisher Scientific). The CC50 values were calculated from best-fit dose response curves with variable slope in Prism 8.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Serum samples at varying dilutions were incubated for 2 h followed by detection by a goat anti-human Fc-HRP secondary antibody (ThermoFisher Scientific). The plasma concentration of AvFc was calculated by interpolating from a standard curve ...
-
bioRxiv - Microbiology 2020Quote: ... was used to detect circulating antibodies for LSDV in serum samples following the manufacturer’s protocol and analysed with the Multiskan FC Microplate photometer (Thermo Scientific™). Infectious virus titrations of PBMC suspension ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Hamster lungs were homogenized and diluted in Hank’s solution with the addition of 2% FCS and antibiotics (streptomycin sulfate and benzylpenicillin sodium salt, 200 U/ml) (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (59 ...
-
bioRxiv - Microbiology 2022Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (59 ...
-
bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
-
bioRxiv - Immunology 2020Quote: ... Activated CD8+ T cells were maintained in DMEM medium (10% FCS) with 25 mM (HG) or 5.6 mM (NG) glucose (Thermo Fisher Scientific). Raji cells were maintained in RPMI 1640 medium (10% FCS ...
-
bioRxiv - Molecular Biology 2021Quote: ... Interactions were visualized by the incubation of tetramethylrhodamine (TRITC) labelled secondary goat anti-mouse IgG antibodies (Fc specific; 1:1000 dilution in binding buffer; Life Technologies) and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Growth of each microbe was assessed by measurement of optical density (OD) readings at 600nm using a Multiskan FC Microplate Photometer (Thermo Scientific). Bacterial density in each well in the parent plate was then normalized individually to an OD600 of 1.0 using sterile filtered M9 buffer ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... iPSCs were cultured in KSR basal medium (GMEM basal medium with 10% KSR, 1% FCS) or N2B27 basal medium (DMEM/F12 and Neurobasal (ThermoFisher Scientific) in a 1:1 ratio ...
-
bioRxiv - Developmental Biology 2021Quote: ... cells were resuspended in buffer (PBS/2% FCS/ 1% penicillin/streptomycin) containing a viability dye from the Sytox series (Life Technologies) or DAPI (Sigma) ...