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Citations for Thermo Fisher :
2651 - 2700 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hippocampal or cortical neurons were plated at 17,000 cells/well (96-well plate) or 1,000,000 cells/well (6-well plate) in neuron media (Neurobasal medium (ThermoFisher 21103049) supplemented with B27 (ThermoFisher 17504044) ...
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bioRxiv - Neuroscience 2021Quote: ... Retinas were then dissected in DPBS and placed on a culture insert (Millicell) in a 6-well plate (Falcon) containing 1.3ml of equilibrated medium: DMEM (supplemented with glutaMAX; Gibco) with 10% FBS (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 0.6 µg KV3.1b plasmid and 0.1 µg eGPF were transfected into HEK-293 cells using Lipofectamine 2000 (Invitrogen Corporation), and recordings were performed after 3 days of incubation at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: DRGN and retinal cells were washed with PBS and total RNA (n = 6-9 wells/treatment) was extracted using Trizol reagent according to the manufacturer’s instructions (Invitrogen). Appropriate primers were used to validate each gene of interest using the specific primers (Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The nucleofected cells were plated on a well of a 6 well plate coated with Geltrex matrix (Life Technologies) and transitioned to Essential 8 media (Life Technologies)1 ...
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bioRxiv - Molecular Biology 2019Quote: ... After approximately 6 hours the cells were washed with phosphate-buffered saline (PBS) and detached with TrypLE (ThermoFisher Scientific). Then ...
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bioRxiv - Cell Biology 2021Quote: 300,000 cells/well were seeded into duplicate wells of 6-well plates (Thermo Fisher Scientific, Cat# 07-200-83) in 2 mL medium ...
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Cancer Biology 2019Quote: ... whose NBD-amino group is placed at carbon 6 and can therefore not be phosphorylated by HKs34 (Thermo Fisher). The cells were then washed with ice-cold phosphate-buffered saline (PBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Chromosomes were counterstained for 20 minutes with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; ThermoFisher Scientific, D3571) in 2x SSC ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were stably transfected into Fischer Rat Thyroid (FRT) cells in 6-well plates using lipofectamine LTX (Thermo Fisher) and OptiMEM (Thermo Fisher ...
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bioRxiv - Systems Biology 2021Quote: The qPCR was conducted in duplicate using 384-well plates on a QuantStudio™ 6 cycler (Thermo Fisher Scientific). The reactions were performed using unlabeled primers and SYBR Green DNA binding dye (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... The qPCR was performed by QuantStudio™ 6 Flex Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA). Elongation Factor 1α (CqEF1α)(XM_021888526.1 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR amplification was performed on QuantStudio 6 Flex Real-Time PCR Systems (Applied Biosystems, Foster City, CA, USA) with the following conditions ...
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bioRxiv - Plant Biology 2021Quote: ... Ribosome was depleted in 6 µg of RNA from each pool using the Dynabeads mRNA purification kit (Ambion, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Ribosome was depleted in 6 µg of RNA from each pool using the Dynabeads mRNA purification kit (Ambion, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Neuroscience 2020Quote: Mouse midbrains (n = 5-6 per group per age) were collected and RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then washed twice with PBS and stained with 4′,6-diamidino-2-phenylindole (DAPI, Thermo Scientific #D1306) diluted 1:200 in 0.5% BSA in PBS ...
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bioRxiv - Molecular Biology 2021Quote: Transient expression of DNA plasmids was carried out in 6 well plates by reverse transfection using Lipofectamine LTX (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the membrane inserts were placed into 6-well plates containing 1.5 mL maintenance medium consisting of F12/DMEM (Gibco) supplemented with N-2 Supplement (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5 μl of 6 μM samples were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies) or native gels (NativePAGE 4-16% Bis-Tris Gel ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.5 μg pMD2.G were transfected into HEK293T cells in 6-well plates by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The volume of siRNA for a final concentration of 15 nM per well was mixed with 6 μL of HiPerfect in 100 μL of Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA), incubated for 10 min and added to cells in media without antibiotics ...
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bioRxiv - Cell Biology 2020Quote: ... and the lineage-positive cells were depleted using a magnetic particle concentrator according to the manufacturer’s instructions (Dynal MPC-6, Invitrogen). To isolate the LSK/LT-HSC fraction by FACS ...
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bioRxiv - Biophysics 2021Quote: ... 5 mM DTT) and then mixed with 50 mM (final 6 mM) disuccinimidyl dibutyric urea (DSBU) (Thermo Fisher Scientific). Crosslinking reaction was incubated on ice for 2 hours and then was quenched by adding 1M (final 50 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Cell Biology 2020Quote: INS-1 832/13 cells were grown in 35-mm dishes and transfected at 50-60% confluency with FRET-based biosensors (2.5 μg plasmid DNA) using Lipofectamine 2000 (6 μl) (Invitrogen). 24 hours post-transfection cells were plated in μ-slide 8 well glass bottom chamber (Ibidi ...
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bioRxiv - Neuroscience 2021Quote: ... specifically designed primers (appropriate miRCURY LNA miRNA PCR assays) and QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative miRNA expression levels between control and mutant cells were quantified using ΔΔCT Method ...
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bioRxiv - Neuroscience 2022Quote: HEK293T cells (ATCC CRL-3216) were transfected in a 6-well plate with 3μg/well of DNA with Lipofectamine 2000 (ThermoFisher) in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 75 µg lysate and 50 µg media samples were proteolysed and labeled with TMT 6-plex according to the manufacturer’s protocol (ThermoFisher). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...
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bioRxiv - Cell Biology 2022Quote: ... Neurons were transfected with the CryBAR optogenetic system (6 µg plasmid/plate) on DIV5 using Lipofectamine LTX reagent (Invitrogen). On DIV7 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by real-time quantitative PCR analyses of the OXTR on a QuantStudio 6 qPCR platform (Life Technologies, USA) using the appropriate Taqman gene expression array (Hs00168573_m1 ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Neuroscience 2022Quote: Primary cultures of hippocampal neurons were established by isolating E16–17 C57BL/6 embryonic hippocampal tissues in HBSS (Gibco) and dissociating tissues in 0.25% trypsin (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were mounted with the ProLong™ Diamond Antifade Mountant with 4’,6-Diamidino-2-Phenylindole (DAPI) (ThermoFisher Scientific). Ki-67 signals were visualized with Nikon microscopy ...
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bioRxiv - Bioengineering 2022Quote: ... with a total reaction volume of 12.5 μL using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Gene specific primers were used to amplify GDNF (forward sequence 5-GCAGACCCATCGCCTTTGAT-3 and reverse 5-CCACACCTTTTAGCGGAATGC-3).
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bioRxiv - Bioengineering 2022Quote: ... Cells were then treated with 6 mM CHIR99021 (STEMCELL; 72052) in DeSR1: DMEM/Ham’s F12 (Thermo Fisher Scientific; 11039021), 1X MEM-NEAA (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... fused with an N-terminal 6×His-tag and cloned into expression vector pPIC9 (Thermo Fisher Scientific, California, USA). Correctness of the resulting constructs was confirmed by DNA sequencing prior to introduction into Pichia pastoris strain GS115 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... A549 cells were embedded in 6-well culture plates at a density of 20.000 cells/well in 0.8% low melting point agarose (Thermo Scientific) on the top of a layer of low melting point agarose at 1.6% ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were affixed using Prolong Gold Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, Cat. # P36935).
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated at 1 x 10^6 cells per well of a 6 well plate or 5 x 10^6 cells per T25 flask in stage 2 media consisting of 47.5% IMDM (Gibco), 47.5% F12 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... coli cells or 6 10e6 Erwinia oleae cells pre-grown for 3.5 h in DMEM with 25 mM HEPES (Invitrogen) were mixed with EDTA (1 mM ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were plated in 6 well plates and transfected with 5 μl Lipofectamine RNAiMAX transfection reagent and siRNAs (Invitrogen) at 50 nM final concentration according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and axial lymph nodes of naïve un-manipulated WT C57BL/6 mice were isolated and processed in RPMI 1640 (Gibco) as above ...