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Citations for Thermo Fisher :
2801 - 2850 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-diamidine-2-phenylindole dihydrochloride And coverslipped using ProLong™ Gold Antifade Mountant (Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Slides with 6 μm sections were mounted in ProLong Glass Antifade Mountant (Thermo Fisher Scientific, Sugar Land, TX, USA) and those with 35 μm sections in SlowFade Glass Soft-set Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were allowed to rest in fresh slicing solution at 4 °C for 30-45 minutes before being mounted on individual semiporous membrane inserts (2 slices per insert) placed in 6 well plates with 1.1mL of culture media [50% Minimal Essential Medium (MEM; Gibco), 25% heat-inactivated horse serum (Gibco) ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Quantitative RT– PCR was performed using the QuantStudio-6 and -7 Flex Real-Time PCR Systems (Thermo Fisher Scientific) with SYBR® Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Plant Biology 2023Quote: ... was employed for reporting successful amplification using a QuantStudio 6 Pro real-time PCR system (Applied Biosystems, Waltham MA) with specific primer pairs for each target NbBBL gene ...
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed by a QuantStudio 6 Flex real-time PCR detection system (Applied Biosystems, Foster City, CA) with SYBR Premix Ex TaqTM II (Tli RNaseH Plus ...
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bioRxiv - Microbiology 2022Quote: Vero cells in 6-well plates were transfected with 1.5 μg/well pQF356 using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Then the cells were seeded in 100 mm plates in medium containing 800 μg/mL G418 to allow colonies to develop for two to three weeks ...
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bioRxiv - Neuroscience 2022Quote: 3D tissue viability was checked at 2 and 6 weeks after seeding following the established Calcein AM (Invitrogen, C1430) staining protocol ...
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bioRxiv - Molecular Biology 2022Quote: Membrane order was assessed using the polarity-sensitive membrane probe Laurdan (6-Dodecanoyl-2-Dimethylaminonaphthalene) (Thermo Fisher Scientific, D250)20 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Bioengineering 2022Quote: ... was buffered to a final concentration of 6 mg/mL at pH 7.4 with 250,000 cells/mL using 10X phosphate buffered saline (PBS) (Thermo Fisher) with phenol red ...
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bioRxiv - Developmental Biology 2022Quote: Lungs from experimental C57BL/6 mice were inflated with an enzyme mix consisting of the following: Leibovitz media (Invitrogen) 2mg/mL of collagenase type 1 and 2mg/mL of elastase (Worthington Biochemical) ...
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bioRxiv - Neuroscience 2024Quote: ... and CHIR 99021 (Cat# 72052) in sterile non-tissue culture treated 6-well plates (Thermo Fisher Scientific, Cat# 150239). NSCs were passaged using either accutase (Stem cell technologies ...
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bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: MEF cells were grown in a 6 well plate seeded at 50–60% confluency in 2 ml Dulbecco’s modified Eagle medium (DMEM, Gibco) containing 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... The optical density at 600 nm (OD600nm) was measured with a Genesys 6 spectrophotometer (Thermo Fisher Scientific, United States). The preculture was next diluted into a baffled shake flask (250 ml ...
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bioRxiv - Plant Biology 2024Quote: DAPI (4′,6-diamidino-2-phenylindole) solution: Dilute 1 mg/ml DAPI (ThermoFisher, Cat. No. 62248, Rockford, IL, USA) to a final concentration of 0.5 µg/ml in 1x PBST (0.1% Tween-20).
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bioRxiv - Plant Biology 2024Quote: ... 8 and 6 hours were then transferred to MS medium containing zeocin with Actinomycin D (Thermo Fisher, J60148.LB0) or zeocin with DMSO (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Cells were collected from one well of a 6-well plate using 700 µL of TRIzol Reagent (Life Technologies) and RNA was extracted using Direct-zol RNA Miniprep (Zymo Research) ...
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bioRxiv - Genetics 2024Quote: ... 2 x 105 cells were seeded in 6-well plates and grown overnight in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) plus GlutaMAX (Thermo Fisher Scientific), supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... and 6 µg of psPAX2 plasmids into 293T cells grown in a 10-cm dish using Lipofectamine 2000 (Invitrogen). The cell culture supernatant was collected 48 h after transfection and used to transduce parental HeLa cells in the presence of polybrene (8 µg/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were functionalized with 0.1M sulfo-succinimidyl 6-(4’-azido-2’-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Cat# 22589, Thermo Scientific) dissolved in HEPES before overnight incubation in collagen type-I solution (Catalogue # A1048301 ...
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bioRxiv - Immunology 2023Quote: ... and the reactions were measured using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). The relative fold change in gene expression compared to the 471_IgG1H_28z CAR-T cells is represented in the graph.
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bioRxiv - Immunology 2023Quote: ... and the reactions were measured using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). The relative fold change in gene expression compared to the 471_IgG1H_28z CAR-T cells is represented in the graph.
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bioRxiv - Physiology 2023Quote: ... the sections were incubated with 4’,6-diamidino-2-phenylindole (DAPI; D1306, Thermo Fisher Scientific; 1:1000 in PBS) for 2min at room temperature to counterstain the nucleus before being washed twice in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel stuck to it were transferred to a 6-well plate with 1 ml of digestion solution (0.5 % Triton X100, 1 mM EDTA, pH 8 (AM9260G, Ambion), 50 mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... The transcribed RNA was then separated on a 6% TBE-Urea (7M urea) polyacrylamide gel (Thermo Fisher Scientific, EC6865BOX) which was post stained with GelRed (Biotium ...
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bioRxiv - Genomics 2024Quote: ... 3 µg of each plasmid was mixed with 6 µl of Plus reagent and 7.5 µl of Lipofectamine LTX (Invitrogen) reagent ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC CRL-1573) were seeded in 6-well plates with Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965092) supplemented with 10 % FBS (Sigma–Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 1:2000 LipidTOX was added along with 0.25 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes) for 15 min ...
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bioRxiv - Bioengineering 2024Quote: Bone marrow-derived mesenchymal stromal cells from C57BL/6 mice (Cyagen) were cultured in MEMα supplemented with GlutaMAX (Gibco) in 15% fetal bovine serum (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse Transfection of rescue plasmid was performed in 6-well plates using the Lipofectamine 3000 reagent (Thermo Fisher Scientific) to the proportions of 5 μL of 2 μg plasmid per well ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and nuclei were stained with 4’-6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and mounted with an aqueous mounting medium (Aqua-Poly/Mount ...
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bioRxiv - Cell Biology 2024Quote: ... Around 1.6-3.2μg of digested DNA was used to synthesise mRNA with the T3 mMESSAGE mMACHINE kit (AM1348, ThermoFisher Scientific). The mRNA product was purified with the RNeasy MinElute Cleanup Kit (74204 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the recombinant pcDNA3.1 plasmid (6 μg/flask) containing mouse Wnt10b cDNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and envelope (pMD2.G; 0.5 µg) plasmids using either FuGENE 6 or Lipofectamine 3000 transfection reagent (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... live/dead staining was carried out using 6 μM syto 9 and 30 μM propidium iodide (BacLight, Molecular Probes). Biofilms were imaged using a Zeiss LSM 700 compact confocal laser scanning microscope using the 40x objective and appropriate fluorescence settings (syto 9=488 nm laser ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa were prepared in 6-well plates 24 hours before the transfection with 4.5.105 cells/well in RPMI medium (Gibco-Invitrogen) supplemented with 10% fetal calf serum (Gibco-Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... were seeded in a 6-well plate at a cell density of 200,000/cm2 in Dulbecco’s Modified Eagle Media (DMEM, Thermo Fisher) supplemented with 15% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2023Quote: ... and rested for ∼6 hours in R15 (RPMI 1640 medium supplemented with 15% FBS, 1% antibiotic-antimycotic [Thermo Fisher Scientific] ...
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bioRxiv - Immunology 2022Quote: ... All samples were prepared in triplicate and run on a QuantStudio 6/7 Flex Real-Time PCR System (ThermoFisher). Primer sequences for designated HHV-6 marker (U31 ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was resuspended in 1 ml of DMEM before adding the mixture to a 6-well plate containing 2 ml of DMEM with 4.5 g/L D-Glucose and L-Glutamine (Gibco) supplemented with supplemented 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: Cultured N2A control and Fmr1 CRISPR/Cas9 KO cells were seeded in 6-well plates overnight and then transfected using 7.5 µl of Lipofectamine 3000 (Invitrogen) and 5 µl of P3000 with the 1 µg of pFlareA-Mapt exon4 splicing reporter ...
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bioRxiv - Bioengineering 2023Quote: ... and liquid agarose (2.5mL) was used to cover the bottom of a high-performance #1.5 6-well plate (Fisher Scientific). This process was repeated for all five wells ...
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bioRxiv - Genomics 2023Quote: ... and 2µL of primers at 3µM in a total reaction volume of 10µL on qPCR was realized on a QuantStudio 6 Flex Real-Time PCR system (ThermoFisher). Each sample was tested in duplicate ...