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Citations for Thermo Fisher :
2851 - 2900 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were stained with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, 1:1000, Invitrogen, Thermo Fisher Scientific, MA) for 10 mins ...
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bioRxiv - Genomics 2023Quote: ... and 2µL of primers at 3µM in a total reaction volume of 10µL on qPCR was realized on a QuantStudio 6 Flex Real-Time PCR system (ThermoFisher). Each sample was tested in duplicate ...
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bioRxiv - Bioengineering 2023Quote: ... and liquid agarose (2.5mL) was used to cover the bottom of a high-performance #1.5 6-well plate (Fisher Scientific). This process was repeated for all five wells ...
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bioRxiv - Pathology 2023Quote: Primary human lung fibroblasts (n = 6 for both control and IPF, all at passage 5) were harvested using 0.25% Trypsin-EDTA (Gibco) and centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with 1µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were stained with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, 1:1000, Invitrogen, Thermo Fisher Scientific, MA) for 10 mins ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was resuspended in 1 ml of DMEM before adding the mixture to a 6-well plate containing 2 ml of DMEM with 4.5 g/L D-Glucose and L-Glutamine (Gibco) supplemented with supplemented 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression was determined by quantitative real-time PCR using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative gene expression was determined using ΔΔCT method ...
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bioRxiv - Neuroscience 2023Quote: ... Organ of Corti explants were isolated from C57BL/6 mice at postnatal day 4 in cold Leibovitz’s L-15 medium (cat. # 21083027, Gibco) and held in place by two glass fibers glued to the bottom of a Petri dish ...
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bioRxiv - Genetics 2023Quote: 3 independent total RNA extractions from 30 ovaries from 3-6-day-old RevI-H2i2 flies using Trizol (Invitrogen) were performed ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded at a concentration of 150,000 cells per well of a Matrigel-coated 6-well plate in E8 medium (ThermoFisher) containing Rock-inhibitor (10 µM ...
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bioRxiv - Biochemistry 2023Quote: ... coverslips were stained for 5 min with 1 μg/ml 300 nM 4′,6-diamidino-2-phenylindole (Life Technologies) to visualize nuclei ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293A cells were initially plated at 750k cells/well in 6-well plates in Dulbecco’s modified eagle media (DMEM) supplemented with 10% fetal bovine serum (FBS) and GlutaMax (Gibco) with 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and 6 µg of PEI were diluted in 0.5 ml of Opti-MEM™ Reduced serum medium (Gibco™) per single well ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were assembled for 20 min at room temperature and separated on 6% DNA retardation gels (Thermo Fisher scientific) at 200 V for 1–2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 1 µg FLAG-AGAL construct and 4.5 µg pOG44 was transfected into HFTs seeded onto a 6-well plate using Lipofectamine 2000 (Invitrogen). After 48 hours the transfected cells were selected with hygromycin (200 µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... 8 days after transfection antibiotic selection was performed using 6 µg mL-1 blasticidin S (Thermo Fisher Scientific, A1113903) to identify cells which had undergone productive editing ...
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bioRxiv - Cell Biology 2023Quote: ... supplied with the primers for ICP27 and β-actin on a Quant Studio 6 Flex qPCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2022Quote: ... and an oxygen-scavenger system (60□mg ml−1 glucose oxidase, 6□mg□ml−1 β-ME (Gibco, 21985023)) were used for all STORM imaging experiments.
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bioRxiv - Developmental Biology 2023Quote: ... Images were collected manually or automatically with 4-6 µs dwell time using Maps 3.11 software (Thermo Fisher Scientific) (Burel et al. ...
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bioRxiv - Bioengineering 2023Quote: ... the retinal sections were mounted with Fluoromount G™ containing 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI, Invitrogen) for nuclei detection ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell pellets were finally resuspended in wash buffer containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) or 7-AAD viability dye (BioLegend ...
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bioRxiv - Developmental Biology 2023Quote: ... resuspended in mounting media containing 4’,6’-diamidino-2-phenylindole (DAPI) (Fluoromount-G™ Mounting Medium, with DAPI, Invitrogen), incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and SEC using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The RT-qPCR was performed using the Promega® 2x Master Mix and the QuantStudio™ 6 (Applied Biosystems) for 40 cycles ...
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bioRxiv - Pathology 2023Quote: ... Cells were spun down at 350g for 6 minutes and were re-suspended with 4mL of 0.05% Trypsin (ThermoFisher) and incubated at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with secondary antibodies for 2 hours at RT in a humid chamber and DNA was counterstained with 0.1 µg/ml of DAPI (DNA intercalator, 4’,6-Diamidino-2-Phenylindole, D1306, Invitrogen). Coverslips were mounted with ProLong Diamond Antifade (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Microbiology 2024Quote: ... The optical density at 600 nm (OD600nm) was measured with a Genesys 6 spectrophotometer (Thermo Fisher Scientific, United States). The preculture was next diluted into a baffled shake flask (250 ml ...
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bioRxiv - Genetics 2024Quote: ... 2 x 105 cells were seeded in 6-well plates and grown overnight in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: Cells were collected from one well of a 6-well plate using 700 µL of TRIzol Reagent (Life Technologies) and RNA was extracted using Direct-zol RNA Miniprep (Zymo Research) ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: MEF cells were grown in a 6 well plate seeded at 50–60% confluency in 2 ml Dulbecco’s modified Eagle medium (DMEM, Gibco) containing 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Total RNA from mating wild-type and EMA2 KO cells were isolated at 6 hpm using TRIzol reagent (Invitrogen). The RNA was treated with Turbo DNase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6) at 40°C with vigorous shaking at 1400rpm for 30 minutes using a thermal mixer (Thermo Scientific). The eluted DNA and previous input samples were then amplified using the PCR Primer Mix 3 for Illumina and the VAHTS HiFi Amplification Mix 2 (ND607 ...
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bioRxiv - Cancer Biology 2023Quote: ... was transduced by lentivirus and successfully transduced cells were selected by 6 μg/ml blasticidin (R21001; Thermo Fisher Scientific) for 1-2 weeks ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with SSCT and incubated with 600 nM 4′,6-Diamidin-2-phenylindol (Dapi, D1306, Thermo Scientific) for 10 minutes before final washing in SSCT ...
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bioRxiv - Physiology 2023Quote: ... the sections were incubated with 4’,6-diamidino-2-phenylindole (DAPI; D1306, Thermo Fisher Scientific; 1:1000 in PBS) for 2min at room temperature to counterstain the nucleus before being washed twice in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and envelope (pMD2.G; 0.5 µg) plasmids using either FuGENE 6 or Lipofectamine 3000 transfection reagent (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 8 and 6 hours were then transferred to MS medium containing zeocin with Actinomycin D (Thermo Fisher, J60148.LB0) or zeocin with DMSO (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: DAPI (4′,6-diamidino-2-phenylindole) solution: Dilute 1 mg/ml DAPI (ThermoFisher, Cat. No. 62248, Rockford, IL, USA) to a final concentration of 0.5 µg/ml in 1x PBST (0.1% Tween-20).
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bioRxiv - Pathology 2024Quote: ... IRB number: VGHUST105-G7-6-1) were cultivated in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was incubated for 15 minutes at room temperature and then separated on a 6% DNA retardation gel in 0.5X TBE running buffer (ThermoFisher). The gel was imaged with a SapphireTM multimode imager in the Keck Biophysics facility at Northwestern University.
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bioRxiv - Cell Biology 2024Quote: ... a 6-well was coated with 1 mL of a 1:200 solution of either Geltrex (Gibco™ A1413202) or Cultrex (Bio-Techne 3445-005-01 ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from samples containing 6 OD600 units of mid-log phase cells using Trizol (Invitrogen #15596026) and chloroform ...
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bioRxiv - Biophysics 2024Quote: ... USA) dissolved in ethanol or 1μL 2.5mg/mL N,N-Dimethyl-6-dodecanoyl-2-naphthylamine (LAURDAN; Thermo Fisher, USA) dissolved in dimethyl sulfoxide to label the EVs ...
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bioRxiv - Cancer Biology 2024Quote: ... typically 1.5 μg of plasmid was reverse transfected into 300,000 293T cells in a well of a 6-well plate using OptiMEM (Gibco) and FuGENE 6 (Promega) ...