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Citations for Thermo Fisher :
2601 - 2650 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... snap-cooled and separated by capillary electrophoresis (CE) at 12kV for 25min using a POP-6 polymer (Thermo Scientific). “One-pot” U-insertion/U-deletion reactions were assembled by annealing of the U-insertion and U-deletion pre-mRNA/gRNA-hybrid RNAs in separate reaction vials ...
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bioRxiv - Cell Biology 2020Quote: ... cells seeded in a 6-well format (0.3×106 cells/2ml/well) were switched to a galactose medium (glucose-free DMEM (Gibco) supplemented with 10 mM D-galactose (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Gene expression was analyzed by real-time qPCR using QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) or Biomark microfluidic system using a 48.48 Dynamic Array IFC for gene expression (Fluidigm ...
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bioRxiv - Cell Biology 2020Quote: ... ovaries from randomly cycling female mice (FVB/N, 6 weeks old) were collected and incubated in 0.25% Trypsin/PBS (Invitrogen) (37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... coated 6-well plates or glass coverslips (for smRNA FISH purposes) in Essential 8 Flex medium (Thermo Fisher Scientific) with E8 supplement (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... then loaded with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethylester (BCECF-AM, 1.6 μM, Life Technologies) for 30 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 2020) and reactions were carried out using a QuantStudio 6 and 7 Flex Real-Time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were mounted on ProLong Gold with 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Delta-Vision II microscope system with an Olympus IX-71 inverted microscope using a 100x objective A ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were incubated in a 10 mM sodium citrate buffer with 0.05 % Tween 20 (pH of 6) (Fisher Scientific) for 15 minutes at 99□ prior to fixation ...
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bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: Drosophila S2 cells from the Drosophila Genomics Resource Center (DGRC - stock#6) were cultured in Schneider’s medium (Thermo Scientific) supplemented with 10% Heat-Inactivated Fetal Bovine Serum (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: One hundred and fifty tergal gland segments (Segment 7) and control segments (Segment 6) of Dalotia males were dissected in EBSS (Earle’s Balanced Salt Solution; ThermoFisher) and transferred to into 150 µl ice-cold Schneider’s Drosophila medium and fetal bovine serum (SDM+FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Frozen sections were cut (6-10 μm) and spotted onto positively-charged slides (Superfrost Plus slides-Thermo Fisher Scientific) and stored at -20°C prior to use ...
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bioRxiv - Cell Biology 2021Quote: ... Wild type and Ex6KO cell lines were plated on a 6-well plastic plate (Nunclon Delta Surface,ThermoFisher Scientific). When confluent ...
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bioRxiv - Molecular Biology 2021Quote: ... Datasets were collected on an Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific). The cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted in ProLong™ Gold Antifade Mountant containing 4’,6’-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific). Images were acquired at 100x magnification using a Nikon Eclipse Ti fluorescence microscope fitted with a Hamamatsu C11440 digital camera ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 ng of end-labeled pNG1175 DNA was mixed with 1 µL streptavidin-functionalized paramagnetic beads (M-270 Dynabeads, diluted 1:6 in 1X PBS with 0.1% BSA; Invitrogen) in 10 µL PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA loading buffer was added and 10 mL of the reactions were electrophoresed on 6% DNA Retardation gels (Invitrogen) at 130 V for 60 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Vero-6 cells (ATCC CRL-1586) were maintained in DMEM medium (Gibco, 4.5 g/L glucose, w/o pyruvate) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Genomics 2020Quote: ... 2 µl cDNA (diluted 1:20) was used in a 6 µl Fast SYBR Green qPCR reaction (ThermoFisher Scientific). No-template controls for each gene were run in all qPCR plates ...
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bioRxiv - Immunology 2021Quote: ... Targeted qPCR was performed using the QuantStudio 6 Real-Time PCR system using the following Taqman Primers (Applied Biosystems): BCL6 (Hs00153368_m1) ...
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bioRxiv - Molecular Biology 2021Quote: ... ESCs were transfected in a single well of a 6-well plate with 0.5μg Cas9 guide plasmid and 2μg targeting construct plasmid using Lipofectamine 3000 (Thermo Fisher Scientific) according to manufacturer’s guidelines ...
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bioRxiv - Zoology 2020Quote: Xela DS2 (passage 74) and Xela VS2 (passage 75) cells were seeded in 6-well plates (BioLite, Thermo Scientific) at cell densities of 300,000 cells/well in 1 mL of complete medium and allowed to adhere overnight at 26°C ...
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bioRxiv - Developmental Biology 2019Quote: HEK cells were grown in 6 well plates and transfected with 4µg of each DNA with lipofectamine 2000 (Invitrogen) for 6hrs according to manufacturers instructions.
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bioRxiv - Biochemistry 2019Quote: ... fluorescent probes Prodan (6-propionyl-2[dimethylamino]-naphthalene) and ANS (1-anilinonaphthalene-8-sulfonic acid) were also from Invitrogen; PMB ...
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bioRxiv - Microbiology 2019Quote: 293T cells in 6-well tissue culture plates were transfected using Lipofectamine2000 according to manufacturer’s instruction (Thermo Fisher Scientific). For scaleup transient transfection of 293S GnTI- cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 6-12 µg anti-HA antibodies (i.e., 12CA5, University of Chicago Monoclonal Antibody Facility) or 6 µg anti-V5 antibodies (R960-25, Invitrogen) per reaction were first conjugated to protein A sepharose beads in a 50% slurry that was first washed in IPP150 ...
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bioRxiv - Cell Biology 2019Quote: ... and oligonucleotides targeting genes of interest (IDT) in triplicate and run on QuantStudio 6 Flex (Applied Biosystems Cat# 4485694) using protocols according to the mastermix manufacturer’s specifications ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were run on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, part of Life Technologies Corporation, CA) according to the manufacturer’s recommendations using a melt protocol in 0.05-degree/sec increments from 25°C to 95 °C ...
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bioRxiv - Genetics 2019Quote: ... 2.4×104 cells were seeded per well of a 6-well cell culture plate containing 4ml of DMEM (Gibco) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670, 0.001% Brij35 ThermoFisher, #28316 ...
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bioRxiv - Genetics 2019Quote: ... about 6 males and 10 female flies were flipped onto a new flask (Fisher Scientific, Cat. No.:AS-355) of food and allowed to mate at 25°C and lay eggs ...
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bioRxiv - Molecular Biology 2019Quote: ... or Tg(EF1α:JPXE1-E3) plasmids per well of a 6-well plate according to the Lipofectamine® 2000 (Invitrogen) manufacturer’s instructions while still in the stem cell state ...
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bioRxiv - Molecular Biology 2019Quote: ... or Tg(EF1α:JPXE1-E3) plasmids per well of a 6-well plate according to the Lipofectamine® 2000 (Invitrogen) manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The cells were then seeded onto Matrigel-coated 6-well plates containing Essential 8™ Medium (ThermoFisher #A15169-01) with added Y-27632 for recovery following nucleofection ...
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bioRxiv - Microbiology 2020Quote: ... Finally samples were counter stained for nucleus with 1 μM DAPI (4′, 6-diamidino-2′-phenylindoldihydrochloride; Thermo Fisher Scientific). Zeiss inverted Axio Observer fluorescent microscope (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: ... dsRNA for knocking-down Par-6 or aPKC was synthesized with MEGAscript T7 Transcription Kit (Ambion, Thermo Fisher Scientific) according to the manufacurer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... dsRNA for knocking-down Par-6 or aPKC was synthesized with MEGAscript T7 Transcription Kit (Ambion, Thermo Fisher Scientific) according to the manufacurer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... 100×103 cells / well cultured in 6 well plates were transfected with 0.8 μg each plasmid DNA using Lipofectamin2000 following the manufacturer’s protocol (ThermoFisher Scientific) and analysed 48 hours later.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... insect cells (cell density at 5 x 105 cells/mL) using 6 μL of CellFectin reagent and 100μL of SFM II media with antibiotics (penstrep, 0.5X) (Gibco™) and allowed to grow for 1 hour at 28°C ...
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bioRxiv - Neuroscience 2020Quote: ... L4-6 ganglia were isolated from adult male Sprague Dawley rats (175-250 g) and dissociated with collagenase (Invitrogen). Cells were washed and then plated with DMEM/F12 (Cellgro ...
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bioRxiv - Cancer Biology 2020Quote: ... Proton transport was measured using ATP-dependent quenching of 9-amino-6-chloro-2-methoxy-acridine (Acridine Orange, ThermoFisher) fluorescence quenching for isolated vacuoles as previously described21
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bioRxiv - Immunology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole and were embedded with ProLong Gold mounting medium (Life Technologies). ImageJ/Fiji software (National Institutes of Health ...
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bioRxiv - Immunology 2021Quote: ... kidneys were wax-embedded and sliced into 6 μm sections onto SuperFrost Plus™ adhesion slides (Thermo Fisher Scientific). Slides were air-dried O/N at RT and ...
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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bioRxiv - Cell Biology 2019Quote: ... hippocampi were dissected from E18 Sprague-Dawley rat embryos or E17 C57BL/6 mice embryos and treated with 0.25% trypsin in HBSS (Invitrogen) for 25 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Cells were reverse-transfected (transfected while plating) in 6-well plates (3.5 -5 × 105 cells per well) using Lipofectamine RNAimax transfection reagent (Invitrogen), following standard protocols ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were mounted in ProLong Gold antifade reagent supplemented with 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes). Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402 ...
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bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and diluted 1:6 with DNAse/RNAse-free water (Thermo Fisher, Waltham, MA, USA). qRT-PCR was performed with SYBR-Green Master Mix (Thermo Fisher Scientific ...