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Citations for Thermo Fisher :
2751 - 2800 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... qPCR reactions were performed in duplicate on an Applied Biosystems Quantstudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) using 4 ng cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
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bioRxiv - Molecular Biology 2019Quote: ... Coverslips were washed in 0.1% IGEPAL/PBS and mounted using ProLong Gold antifade reagent with 4’-6-Diamidino-2-phenylindole (DAPI) (Invitrogen). Immunofluorescence studies in HeLa cells were carried out as described above ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Cell Biology 2019Quote: ... Mounting medium contained DAPI (4’, 6-diamidino-2-phenylindole) to visualize the nucleus (Molecular Probes Inc., Eugene, OR, USA). Negative controls were performed with either no primary or no secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... incubated 5 minutes with at room temperature with 500 ng/ml 4’,6-Diamidino-2-Phenylindole Dilactate (DAPI; ThermoFisher) in DPBS and mounted in ProLong®Gold (ThermoScientific).
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bioRxiv - Physiology 2021Quote: ... Cells were treated with dexamethasone (1μM) or vehicle (0.01% ethanol) and lysed 6 or 24 hours later by adding 0.5ml TRIzol (Invitrogen, ThermoFisher, UK) following removal of medium ...
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bioRxiv - Physiology 2021Quote: ... Cells were treated with dexamethasone (1μM) or vehicle (0.01% ethanol) and lysed 6 or 24 hours later by adding 0.5ml TRIzol (Invitrogen, ThermoFisher, UK) following removal of medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat-induced antigen retrieval was performed by incubating slides in a solution of 10 mM sodium citrate pH 6 (MilliporeSigma; SX0445-20) with 0.05% (v/v) Tween20 (ThermoFisher) at 98C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... For siRNA experimentsHEK293T cells (1.5×105 cells/ml) were seeded in 6-well plates and transfected with 150nM siRNA using 4μl Lipofectamine 2000 (Invitrogen) and 184μl OptiMEM ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids expressing TSC22D4 alleles and Akt1 were transiently co-transfected to Hepa1-6 cells using Lipofectamine 2000 (Invitrogen, #11668019) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... newly formed clusters were transferred into Low-attachment 6-well plates (Cat. No. 07-200-601, Thermo Fisher Scientific) and cultured with RPMI 1640 plus GlutaMAX + 1% (v/v ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Cell Biology 2021Quote: ... For transfection, 375 ng of synthetic sgRNA (TrueGuide™, CRISPR932782_SGM) along with 6 μL Cas9 mRNA (Invitrogen™, A29378), and 18 μL of the MessengerMAX™ reagent (Invitrogen™ ...
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bioRxiv - Cell Biology 2019Quote: ... at a ratio of 1 μg eYFP-PCNA to 0.1 μg hygromycin marker to 6 μl FuGENE6 according to the manufacturer’s instructions except that we washed cells with Opti-MEM (Invitrogen), transfected cells in Opti-MEM ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were seeded at low and high density in 6-or 12-well plates coated with 0,2% type 1 Collagen (Gibco) and grown in PM for 24 hours ...
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bioRxiv - Genetics 2021Quote: ... and Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) plus GlutaMax (Thermo Fisher), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... NALM-6 cells were cultured in RPMI media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco). MCF7 cells were cultured in DMEM media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (4.4 g/L MS salts mixture, 10 g/L D-sucrose) in 6 well-plates (Nunc) and allowed to acclimate for 48 hours with mild agitation (130 rpm).
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bioRxiv - Microbiology 2021Quote: ... MDCK cells were grown to 90-95% confluence in 6-well tissue culture plates in DMEM (ThermoFisher Scientific/Gibco) containing 10% FBS (ThermoFisher Scientific/Gibco ...
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bioRxiv - Microbiology 2020Quote: 4 μl of purified spores (6×107 spores/ml) were mixed with 40 μl of NucBlue (Invitrogen, catalog #R37605) and incubated at 25°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: Bone marrow derived macrophages (BMDMs) were generated from 6-8 week old BALB/c mice in RPMI-1640 (Gibco) containing 10% foetal calf serum ...
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bioRxiv - Molecular Biology 2021Quote: ... 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 6% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco, 15240062). T cell lines were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Microbiology 2021Quote: ... were used to amplify cellular RNA and viral RNA by QuantStudio 6 Flex Real-Time PCR Systems (Applied Biosystems). The relative expression levels of SARS-CoV-2 N gene was calculated using the standard curve method and normalized to 18S ribosomal RNA as an internal control.
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bioRxiv - Immunology 2020Quote: 250 μL K92 peptide at 1 mg/mL was reduced with 6 μL of 0.5 M Dithiothreitol (Thermo Fisher) for 40 minutes at 37 °C and alkylated with addition of 18 μL of 2-Iodoacetamide (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... Dissociated cells and remaining pieces of tissue were placed in a single well of a 6-well plate containing 2 mL of Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 20% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Genetics 2021Quote: ... The samples were either frozen (n = 6) or stored in the RNAlater reagent (Thermo Fisher Scientific, Waltham, MA, USA) (n = 4).
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with 0.2 μM of 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Life Technologies, Carlsbad, CA) in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with 0.2 μM of 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Life Technologies, Carlsbad, CA) in PBS pH 7.4 ...
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bioRxiv - Genetics 2019Quote: Beginning at week 2 post cloning and continuing until week 6 the Phusion Blood Direct PCR Kit (Thermo Scientific) was utilized to identify positive clones ...
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bioRxiv - Genomics 2020Quote: ... standards (3 x 10μl) and samples (6 x ∼18μl) were loaded on a 4-12% Bis-Tris Nu-PAGE (Life Technologies) gel for electrophoresis ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Immunology 2022Quote: ... The presence of SARS-CoV-2 RNA in these samples was evaluated by one-step RT-qPCR in a final volume of 12.5LμL per reaction in 384-wells PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: HeLa cells were grown to 80% confluency in 6-well plates and transfected with 300 ng pcDNA3.1-FLAG-RBM39 constructs using Lipofectamine2000 (Invitrogen). On the following day ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa RAD54KO cells were transfected with 3μg of RAD54-GFP or eGFP plasmid per well in a 6-well plate using Lipofectamine LTX PLUS (Invitrogen). Stable cell lines were generated by selecting with neomycin (800μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.5×105 4T1 cells were co-cultured in 6 wells plates using Transwell plates (0.4 μm, Nunc, ThermoFisher Scientific) with Gr1+CD11b+ sorted cells (top ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.5×105 4T1 cells were co-cultured in 6 wells plates using Transwell plates (0.4 μm, Nunc, ThermoFisher Scientific) with Gr1+CD11b+ sorted cells (top ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were incubated at 37°C for 30 min and loaded onto 6% DNA retardation gels (Thermo Fisher Scientific). DNA was detected by staining the gel with GelRed (VWR) ...
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bioRxiv - Cancer Biology 2022Quote: Total cellular RNA was extracted post-treatment in HT-29 cells (1.5×105/well) plated in 6-well plates (Nunc) at indicated time-points ...
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bioRxiv - Biophysics 2022Quote: ... thoracic and lumbar DRGs were harvested from 4 to 6 week old MrprD-GFP mice and transferred to Hank’s buffered saline solution (HBSS) (Invitrogen). Ganglia were treated with collagenase (2 mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: Modified hESCs were cultured and propagated on Matrigel-coated 6-well culture dishes in Stem Flex medium (Thermo Scientific) supplemented with 100 U/ml Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSC at day 6-7 after passaging were treated with a 1:1 mixture of TrypLE Select (Life Technologies) and 0.5 mM EDTA/phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Genetics 2022Quote: ... Coverslips were mounted with Prolong gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI, P36931, Thermo Fisher Scientific) and analyzed under a Zeiss LSM 510 Meta Confocal microscope (Carl Zeiss ...
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bioRxiv - Microbiology 2022Quote: ... 60 °C for 1 min) X 49 cycles on the QuantStudio 6 flex Real-time PCR system (Applied Biosystems), using the TaqMan RNA-to-CT 1-step kit (Applied Biosystems).
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bioRxiv - Microbiology 2022Quote: ... or 6 µL of H2O (negative control) and run in a StepOne™ Real- Time PCR System (ThermoFisher scientific) with the following program ...
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bioRxiv - Molecular Biology 2022Quote: ... 6 replicates of each condition were treated with DMSO or 100 nM dTAG13 (Tocris Bioscience, Fisher Scientific 66-055) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... adult WT C57BL/6J mouse testes were dissected and incubated at 37 °C for 6 h in either Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12, Thermo Scientific) or compound 5 (100 μM ...
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bioRxiv - Neuroscience 2022Quote: ... of 0.5% low-melting agarose (FMC) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-diamidine-2-phenylindole dihydrochloride And coverslipped using ProLong™ Gold Antifade Mountant (Invitrogen by Thermo Fisher Scientific).