Labshake search
Citations for Thermo Fisher :
2501 - 2550 of 4593 citations for 6 Chloronicotinohydrazide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2024Quote: ... Resulting libraries were size selected for 145-160bp fragments using Novex 6% TBE gels (Invitrogen, Cat. No. EC6265BOX) to retain only the small RNA inserts ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with 6 nM siRNA mixed with OptiMEM reduced serum medium (Thermo Fisher Scientific, Cat# 31985062) using Lipofectamine RNAiMAX transfection reagent (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... RNA yield and integrity was verified using a NanoDrop spectrophotometer and a Novex 6% TBE-urea gel (Invitrogen), respectively.
-
bioRxiv - Biochemistry 2024Quote: ... 10 µL of this mix was added into wells of a 12-well 6% DNA retardation gel (Invitrogen) that was pre-running for at least 30 minutes in 0.5X TBE at 150V ...
-
bioRxiv - Microbiology 2024Quote: ... Beads were washed 6 times with 1 mL each of ChIP buffer on a magnetic tube rack (Invitrogen). After removing the final wash ...
-
bioRxiv - Cancer Biology 2023Quote: ... All samples were run in triplicate on a Quant Studio 6 Flex machine (Applied Biosystems, Waltham, MA, USA) and analyzed using QuantStudio Design and Analysis Software (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: ... saquinavir 40 μM or 20 μM) for 6 h and lysed in 0.15% w/v saponin (ThermoFisher Scientific) for 10 min on ice ...
-
bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...
-
bioRxiv - Developmental Biology 2023Quote: ... ATs and TTs were fixed in 4% PFA and stained with 4′,6- diamidino-2-phenylindole (DAPI) (Invitrogen) to identify cell nuclei ...
-
bioRxiv - Genomics 2023Quote: ... Cells were then co-transfected with the two plasmids at a molar ratio of 6:1 (2.5 μg of homology vector, ∼0.7 μg of sgRNA vector) using Lipofectamine2000 (ThermoFisher). The next day ...
-
bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, USA).
-
bioRxiv - Pathology 2023Quote: ... Culture identity was confirmed using species-specific qPCR assays on the Quantstudio 6 platform (Applied Biosystems, MA USA); see Table S1.
-
bioRxiv - Molecular Biology 2022Quote: ... HeLa were prepared in 6-well plates 24 hours before the transfection with 4.5.105 cells/well in RPMI medium (Gibco-Invitrogen) supplemented with 10% fetal calf serum (Gibco-Invitrogen) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected as follows: 2 μg eGFP-SHLD3 constructs and 6 μl Lipofectamine 2000 (Thermo Scientific) were incubated in Opti-MEM for 20 min at room temperature before being added to the cells whose media had been changed to McCoy’s with 10% FBS and no antibiotics ...
-
STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... mounted with 10 µL of ProLong Diamond with DAPI (4’,6-diamidino-2-phenylindole; Molecular Probes, Eugene, OR), and incubated for 24-48 hours prior to analysis ...
-
bioRxiv - Microbiology 2023Quote: ... and counterstained with 4′,6-diamidin-2-phenylindole (DAPI, 4 μg mL-1, Thermo Fisher Scientific, MA, USA). From the 18 biofilms from each year ...
-
bioRxiv - Plant Biology 2023Quote: ... and qPCR was performed using the indicated oligos (Table S4) (QuantStudio 6 Pro PCR system; Thermo Fisher Scientific) using Power SYBR Green Master Mix (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2023Quote: ... droplets were put in 6-well Ultra Low Attachment Plates (Thermo Fisher Scientific P6 : REF 150239 “untreated Nunc”) in Expansion Medium without vitamin A (for 50 mL ...
-
bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed by running the following thermocycler program on a QuantStudio 6 Flex system (Applied Biosystems): 50C for 15 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... Gut orientation was analyzed by treating MZ myo1dpt31cand wildtype larvae with 0.3 μg/ml PED-6 (ThermoFisher #D23739) for 3 hours at 6 days post fertilization as described88 ...
-
bioRxiv - Biophysics 2023Quote: ... Sample were rinsed in PBS and incubated with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Scientific™, USA) for 5 mins and Alexa 594-phalloidin (Invitrogen™ ...
-
bioRxiv - Bioengineering 2023Quote: ... HUVECs were cultured for 6-7 days prior to detachment with 0.25% trypsin-EDTA (Life Technologies, 25200-056) for use in experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no ...
-
bioRxiv - Neuroscience 2023Quote: ... 6-well plate (500x103 cells/well) in Neural Expansion Medium (NEM: 49% Neurobasal, 49% Advanced DMEM (Thermo Scientific), 2% Neural Induction Supplement ...
-
bioRxiv - Neuroscience 2023Quote: ... EBs were seeded on one well of a 6-well plate coated with poly-Ornithine/Laminin (Thermo Scientific). Medium was changed every other day ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were incubated in Cellvis 6 Well glass bottom plates with high performance #1.5 cover glass (Fisher Scientific). For control wells to be pre-treated with cathepsin inhibitors ...
-
bioRxiv - Cell Biology 2023Quote: ... HSPCs were incubated with 20µM NBD C6-Ceramide (6-((N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl)Sphingosine) (Invitrogen) for 30 minutes at 4°C for Golgi staining or Cytopainter (Abcam ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... approximately 10 female and 6 male flies were placed into a flask (Fisher Scientific, Cat. No.:AS-355) of food and maintained at 25°C for one week to allow for mating and egg laying ...
-
bioRxiv - Cancer Biology 2023Quote: ... This insert creates a clean cell-free gap in a 6-or 12-well plate (Thermo Scientific, 150239). The assay was performed according to the manufacturer’s protocol (ibidi) ...
-
bioRxiv - Cancer Biology 2023Quote: ... B6.Cas9 keratinocytes(see above) were plated in 6-well plate (Thermo Scientific Nunclon TM Delta Surface; 140675) at 1.5 × 105 cells per well and infected with 100-300μl of low-titer virus in the presence of infection mix (1/10 dilution of polybrene [10 mg ml−1 Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... U2OS cells were infected (24 h) in viral mixture (1.5 mL DMEM, 1.5 mL viral harvest, 6 µL polybrene, Invitrogen) and DMEM with 7.5 µg/mL blasticidin (Sigma ...
-
bioRxiv - Genomics 2023Quote: ... All nuclei were pooled and stained with DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306). Using a FACSAria III cell sorter (BD Biosciences) ...
-
bioRxiv - Genomics 2023Quote: ... and 6 mg magnetic beads (per sample) were conjugated overnight using the Dynabeads Antibody Coupling Kit (Invitrogen, 14311D). The next day ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were then washed 6× and 50 µl of 1-Step™ 3,3’,5,5’-tetramethylbenzidine (TMB; Thermo Fisher) substrate was added to each well and incubated for 3 min ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Grids were blotted for 4-6 seconds with blotforce of 15 on a Vitrobot Mark IV Vitrobot (ThermoFisher) and directly plunged into liquid-nitrogen-cooled liquid ethane.
-
bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained by incubation with 0.5 – 1 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, D1306, Invitrogen) in PBS for 10 min at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... The resulting pellet was resuspended and differentiated for 6 days in culture media containing: DMEMF12 (Gibco, #11330-032) supplemented with 20% FBS (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... thetaiotaomicron using a Applied Biosystems QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, Foster City, CA, USA), primer sets BT2156-BT2160 ...
-
bioRxiv - Neuroscience 2023Quote: Eight-micron sections of L4-6 DRG were blocked for two hours in Superblock (ThermoFisher; Grand Island, NY), 1.5% Normal Donkey Serum ...
-
bioRxiv - Immunology 2023Quote: ... when floating cells were collected and plated into new 6 cm dishes with Maturation Media (RPMI (Gibco, 61870036), 10% FBS ...
-
bioRxiv - Cell Biology 2023Quote: ... 800,000 cells were seeded into a 6-well plate (NuncMicroWell Plates with Nunclon; ThermoFisher Scientific, Waltham, MA, USA). At day 0 the transfection was done ...
-
bioRxiv - Developmental Biology 2023Quote: ... were isolated from embryos incubated for between 6 (E6.0) and 8 (E8.0) days and maintained in chilled Leibovitz’s L-15 media (GIBCO, Invitrogen). Papillae were dissected as described previously 65 and cultured nerve-side-down on Millicell cell culture inserts (Millipore ®) ...
-
bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
-
bioRxiv - Bioengineering 2023Quote: ... Droplets of a 5 μL cell-Matrigel mixture were manually deposited onto a high-performance #1.5 glass-bottom 6-well plate (0.170±0.005 mm) (Fisher Scientific). The datasets were separated into non-treatment and treatment groups ...
-
bioRxiv - Neuroscience 2023Quote: ... transfections were performed directly after splitting the cells in 6-well plates using Lipofectamine 2000 Reagent (Invitrogen, 11668019) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... qPCR reactions were run in a QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher, Cat. No. 4485691) and Ct values were obtained using built-in software ...
-
bioRxiv - Microbiology 2023Quote: ... All RT-qPCR experiments were performed with a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific).
-
bioRxiv - Biophysics 2023Quote: ... in 3% BSA with a 1:50 ratio and 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) were employed ...
-
bioRxiv - Immunology 2022Quote: ... The resulting monocytes were then differentiated with 50 ng/ml human M-CSF (Miltenyi) for 6 days in Iscove’s modified Dulbecco’s medium (Gibco) containing 5 % fetal calf serum (CAPRICORN ...