Labshake search
Citations for Thermo Fisher :
2551 - 2600 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... RNA Polymerase II 8WG16 (Covance #MM2-126R, 2 µg/ChIP) and Phospho-RNA pol II CTD (Ser5) (Thermo Fisher Scientific #MA1-46093, 2 µg/ChIP). ChIP assays for qPCR quantification were performed using primers shown in Table 2 ...
-
bioRxiv - Plant Biology 2021Quote: ... The primers RE-AMI-F and RE-AMI-R were used to amplify the original amiRNA and inserted it into pDONR221® using BP Clonase II® and then recombined into pFH0032 (Hauser et al.,2013; Hauser et al., 2019) using LR Clonase II® (Invitrogen, Carlsbad,CA, USA). (Supplemental Table 1).
-
bioRxiv - Immunology 2023Quote: ... The supernatant was discarded and the protein pellet was air dried before resuspend in triethyl ammonium bicarbonate (TEAB, Thermo Scientific, USA, # 90114; 100 mM, 200 µL). The total isolated protein from splenic CD4+CD44+ cells was quantified using Bicinchoninic acid (BCA ...
-
bioRxiv - Biophysics 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
-
bioRxiv - Cancer Biology 2021Quote: ... The supernatant was sent to identify differentially expressed proteins by reversed phase nano-LC-MS/MS (Ultimate 3000 coupled to a Q-Exactive HF, Thermo Scientific) at the Rockefeller University Proteomics Resource Center.
-
TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... Phase-contrast images were collected with a 20× objective every 30 s using an EVOS FL Auto (Thermo Scientific, Waltham, MA) and transferred to ImageJ to generate movies.
-
bioRxiv - Cell Biology 2020Quote: ... HPLC analysis was performed employing a C30 reverse-phase column (Thermo Acclaim C30, 2.1 x 250 mm, 3 µm, operated at 40° C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC (Agilent ...
-
bioRxiv - Microbiology 2020Quote: ... Peptide samples were fractionated online using reversed phase chromatography followed by tandem mass spectrometry analysis on a Thermofisher Q-Exactive™ mass spectrometer (ThermoFisher). Data analysis was performed using the IP2 suite of algorithms (Integrated Proteomics Applications) ...
-
bioRxiv - Microbiology 2021Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... nanoflow reverse-phase LC-MS/MS was performed using an Eksigent nanoLC2D system coupled to a LTQ Orbitrap-Velos mass spectrometer (Thermo Fisher). Data acquisition was performed using XcaliburTM (Version 2.1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... All ten vials of samples were combined and approximately 100 μg peptide digests from this mix were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to instructions from manufacturer ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... solid-phase reversible immobilization magnetic bead purification (Clean NGS, LABGENE Scientific SA) was performed on a multidrop automated pipetting station (Thermo Fisher).
-
bioRxiv - Physiology 2022Quote: ... An aliquot of the polar phase was collected and vacuum-dried at 4°C and subsequently derivatized with 2% (w/v) methoxyamine hydrochloride (Thermo Scientific) in pyridine for 60 min following by 30 min sialyation N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA ...
-
bioRxiv - Biochemistry 2022Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
-
bioRxiv - Biochemistry 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
-
bioRxiv - Neuroscience 2021Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 150 μL of the upper aqueous phase was transferred to a new microcentrifuge tube and mixed with 225 μL 100% ethanol (cat. no. BP2818500, Fisher Scientific) by gently pipetting 4X ...
-
bioRxiv - Cell Biology 2022Quote: 1μg-equivalent of peptides from each sample was dissolved in 15uL 0.1% formic acid and injected and separated by a capillary C18 reverse-phase column of the mass-spectrometer-linked Ultimate 3000 HPLC system (Thermo Fisher) using a 90-minute gradient (5-85% acetonitrile in water ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Separation of peptides was performed by C18 reverse-phase chromatography at a flow rate of 300 nL/min using a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
-
bioRxiv - Biochemistry 2020Quote: ... The site of phosphorylation of 32P-labeled peptides was determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer of the peptide coupled to Sequelon-AA membrane (Applied Biosystems) as described previously [40].
-
bioRxiv - Developmental Biology 2020Quote: The samples were separated by reversed phase chromatography (75 um × 250 mm PepMap C18, particle size 5 um, Thermo Fisher Scientific), developing a linear gradient from 2% to 80% acetonitrile in 0.1% formic acid within 60 minutes (RSLC nano ...
-
bioRxiv - Developmental Biology 2020Quote: ... 100 µm × 2 cm) and separated in a C18 reversed phase Easy-spray column (2 µm particle size, 75 µm × 50 cm) (ThermoFisher Scientific) at a flow rate of 300 nl/min ...
-
bioRxiv - Developmental Biology 2020Quote: ... Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1500 m/z were performed at 120K resolution (at 200 m/z ...
-
bioRxiv - Genetics 2021Quote: ... Peptides were prepared according to TMT protocol and AQUA stable isotope polypeptides were added to each sample as internal standard reference.Tryptic peptide was loaded onto the C18 stage tip for desalination prior to reversed-phase chromatography on the Easy NLC-1200 system (Thermo Scientific). Gradient liquid chromatography with acetonitrile for 1 h ...
-
bioRxiv - Immunology 2021Quote: ... with pre-packed 0.75mm x 150mm Acclaim Pepmap C18 reversed phase columns (2μm pore size, Thermo) and analyzed on a Q Exactive Plus (Thermo Fisher Scientific) mass spectrometer ...
-
bioRxiv - Systems Biology 2020Quote: ... Eluted peptides were loaded onto a 50 cm long EASY-spray reverse phase column and analyzed on an EASY-nLC-1000 HPLC system (Thermo Scientific) coupled to a Q-Exactive Plus MS (Thermo Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... Eluted peptides were loaded onto a 50 cm long EASY-spray reverse phase column and analyzed on an EASY- nLC- 1000 HPLC system (Thermo Scientific) coupled to a Q-Exactive Plus MS (Thermo Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... Peptide samples were loaded on a split-triple-phase fused-silica micro-capillary column and placed in-line with a linear ion trap mass spectrometer (LTQ) (Thermo Scientific), coupled with a Quaternary Agilent 1100 Series HPLC system ...
-
bioRxiv - Biochemistry 2020Quote: ... Peptides were separated over a 50 cm EasySpray reversed phase LC column (75 µm inner diameter packed with 2 μm, 100 Å, PepMap C18 particles, ThermoFisher). The mobile phases (A ...
-
bioRxiv - Cancer Biology 2020Quote: ... followed by peptide analysis of the digested gel bands by C18 reversed-phase chromatography using an UltiMate 3000 RSLCnano System (Thermo Scientific) equipped with an Acclaim PepMap C18 column (Thermo Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... samples were loaded onto a 25 cm reversed-phase column (75 mm inner diameter; Acclaim PepMap 100 C18; Thermo Fisher Scientific) and separated with an UltiMate 3000 RSLC system (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... 2 μm Acclaim PepMap reverse phase column (Thermo) at 300 nL/min using an UltiMate 3000 RSLCnano HPLC (Thermo Fisher Scientific). Peptides were eluted into a Thermo Orbitrap Fusion mass spectrometer using a linear gradient from 96% mobile phase A (0.1% formic acid in water ...
-
bioRxiv - Neuroscience 2020Quote: ... Each peptide fraction was separated by reverse phase chromatography on a capillary column and analysed online on a hybrid linear ion trap Orbitrap (LTQ-Orbitrap XL, Thermo Scientific) mass spectrometer for identification and relative quantification of pair peptide sequences ...
-
bioRxiv - Physiology 2021Quote: ... analysis was undertaken using a Dionex Ultimate 3000 nano-ultra reversed-phase HPLC system with on-line coupling to a Q Exactive High Field (HF) mass spectrometer (Thermo Scientific). Samples were separated on an EASY-Spray PepMap RSLC C18 column (500 mm⍰×⍰75 μm ...
-
bioRxiv - Plant Biology 2021Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
-
bioRxiv - Biochemistry 2021Quote: ... peptides were separated on a reversed-phase Easy Spray C18 column (50 cm x 75 μm ID, 2μμm, 100 Å, Thermo Scientific). A 4-76% acetonitrile gradient in 0.1% formic acid (total time 140 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... equipped with a reverse-phase column (EASY-Spray column, 50 cm × 75 µm ID, PepMap RSLC C18, 2 µm, Thermo Scientific). Peptides separation was performed at a constant flow rate of 250 nL/min and using a gradient from 0% to 50% of mobile phase B (mobile phase A ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 μL of the peptide solution were chromatographed on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 0.1% formic acid/ 2% acetonitrile (Buffer A ...
-
bioRxiv - Biochemistry 2020Quote: ... samples were briefly centrifuged at 13,000 g and peptide-containing supernatant was used for further analysis Peptides were fractionated into eight fractions using High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher). Peptide fractions were dried in SpeedVac (Eppendorf ...
-
bioRxiv - Biochemistry 2020Quote: Individual fractions of the tryptic peptides were separated by a capillary C18 reverse phase column of the mass-spectrometer-linked Ultimate 3000 HPLC system (Thermo Fisher) using a 90 min gradient (5%-85% acetonitrile in water ...
-
bioRxiv - Cell Biology 2021Quote: ... The pooled samples were then fractionated into 8 fractions using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific 84868), then dried by speed-vac ...
-
bioRxiv - Cell Biology 2021Quote: Lipids were separated using the Thermo Acclaim C30 reverse-phase column (ID 2.1 x 250 mm, 3 μM pore size) (Thermo Fisher Scientific) connected to a Dionex UltiMate 3000 UHPLC system and a Q-Exactive orbitrap mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2020Quote: Extracted lipids were resuspended in methanol and loaded on a reverse phase column connected to a NanoAcquity UPLC system (Waters) and interfaced with a Velos Orbitrap mass spectrometer (Thermo Fisher) (Dautel et al. ...
-
bioRxiv - Plant Biology 2020Quote: ... and tryptic peptides were loaded on stage tips of C18 for desalting prior to reversed-phase chromatography on one of the nLC-1200 easy systems (Thermo Scientific). Then ...
-
bioRxiv - Plant Biology 2020Quote: Synthetic Arabidopsis RALF1 polypeptide was synthesized by using 9-fluorenylmethyl chloroformate solid-phase chemistry with a peptide synthesizer from Thermo Scientific company and confirmed by MALD-TOF analysis (Applied Biosystems Voyager System 2098 ...
-
bioRxiv - Plant Biology 2020Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap C18, 5 μm, 100 μm × 2 cm, Thermo Fisher Scientific) connected to an analytical column (Acclaim PepMan 100 ...
-
bioRxiv - Microbiology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... of supernatant were diluted with high pressure liquid chromatography mobile phase and injected into a reverse-phase system consisting of a C18 column (HR-80 Catecholamine 80 x 4.6 mm, Thermo Scientific, USA) and a 0.1 M NaH2PO4 mobile phase containing 1-octanesulfonic acid (2.75 mM) ...
-
bioRxiv - Microbiology 2021Quote: Epimastigotes in the exponential growth phase were fixed in 70% ethanol overnight at −20°C and treated with 10 μg/mL RNase A (Thermo Scientific) and 40 μg/mL propidium iodide (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2021Quote: ... the dried organic phase was measured using ultra-performance liquid chromatography coupled to a Q-Exactive mass spectrometer (Thermo Fisher Scientific) in positive mode ...